Abstract: Describe is a method for screening mutant prokaryotic cells to identify producers of a cytotoxic agent active against a target cell, the method comprising the steps of: (a) providing cells of a producer prokaryotic species; (b) generating a pool of mutant producer cells by transposon mutagenesis of the cells of step (a) with an activating transposon (TnA), wherein the TnA comprises an outward-facing promoter (TnAP) capable of increasing transcription of a gene at or near its insertion site in the DNA of said producer cells; (c) co-encapsulating individual members of the pool of step (b) with one or more target cells in microdroplets, the microdroplets comprising a volume of aqueous growth media suspended in an immiscible carrier liquid, thereby generating a library of microdroplets each comprising a single mutant producer cell and one or more target cell(s); (d) incubating the microdroplet library of step (c) under conditions suitable for co-culture of the single mutant producer cell and target cell(s) to pro

Abstract: Provided are methods for identifying differentially represented genes, the method comprising the steps of generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter such that transposon insertion into bacterial DNA increases the transcription of a gene at or near the insertion site; growing bacteria from the mutant pool in the presence of different amounts of said antibiotic to produce two or more test cultures; and comparing the distribution of TnA insertions between test cultures.

Abstract: Disclosed is a method for characterizing the effect of an antibiotic on a bacterium, the method comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis of a culture of said bacterium with an activating transposon (TnA) which comprises an outward-facing promoter (TnAP) capable of increasing transcription of a gene at or near its insertion site in the DNA of said bacterium; (b) growing bacteria from the mutant pool in the presence of different amounts of said antibiotic to produce two or more test cultures; (c) sequencing mRNA transcripts produced by TnAP in each of said test cultures to produce an mRNA transcript profile for each of the test cultures; and (d) comparing the mRNA transcript profiles of the test cultures.

Abstract: Disclosed is a process for producing a mutant bacterium which exhibits improved survival and/or growth under a selected growth condition, the process comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises an outward-facing promoter (TnAP) capable of increasing transcription of a gene at or near its insertion site in the DNA of said bacterium; (b) growing bacteria from the mutant pool under the selected growth condition and under one or more reference conditions to produce two or more test cultures; and (c) sequencing mRNA transcripts produced by TnAP in each of said test cultures to produce an mRNA transcript profile for each of the test cultures; and (d) comparing the mRNA transcript profiles of the test cultures to identify a first class of genes which are disadvantageous for growth and/or survival under the selected growth condition and a second class of genes which are advantageous for growth and/or surviva

Abstract: Methods, systems, apparatuses, and computer-readable mediums are described for techniques for supporting telematics-enhanced emergency calls from mobile phones. In some aspects, a method for wireless communication may include establishing, by a user equipment (UE), a personal telematics-enhanced emergency call to a public safety answering point (PSAP), wherein the UE is capable of transmitting telematics data. The method may also include transmitting information that distinguishes the personal telematics-enhanced emergency call from other types of emergency calls.

Abstract: Some embodiments described herein relate to a method that can include receiving, at a first time, first data for routing to a group. The first data can be sent to the group via a first multicast address that is based, at least in part, on a first key associated with a first time period and derived from a first shared secret. At a second time, second data for routing to the group can be received. The second data can be sent to the group via a second multicast address that is different from the first multicast address. The second multicast address can be based, at least in part, on a second key associated with a second time period and derived from a second shared secret.

Abstract: Methods, systems, and devices are described for communicating telematics data and metadata. A first device transmits a first signaling message to a second device over a communication session signaling protocol. The first signaling message includes at least a first set of session information related to a communication session between the first device and the second device and a first set of telematics data for the first device. The first device receives a second signaling message from the second device over the communication session signaling protocol. The second signaling message includes metadata based on a content of the first set of telematics data transmitted in the first signaling message.

Abstract: Provided herein are methods, and related compositions for identifying a putative essential gene which serves as an antibiotic target in a bacterium, the method, in some embodiments, comprising (a) generating an antibiotic resistant mutant of the bacterium by a method comprising the step of selecting for growth in the presence of an antibiotic to produce an antibiotic resistant mutant clone (AbR mutant); (b) transforming the AbR mutant with one or more essential genes of the bacterium and a transposon which insertionally inactivates bacterial DNA to produce a pool of transposon mutants which are merodiploid for the one or more essential genes; (c) growing bacteria from the merodiploid pool in the presence of different amounts of the antibiotic to produce two or more test cultures; and (d) comparing the distribution of transposon insertions between the test cultures to identify a putative essential gene serving as a target of the antibiotic in the bacterium.

Abstract: A modular system for building underwater robotic vehicles (URVs), including a pressure vessel system, modular chassis elements, a propulsion system and compatible buoyancy modules. The pressure vessel system uses standardized, interchangeable modules to allow for ease of modification of the URV and accommodation of different internal and external components such as sensors 4800 and computer systems. The system also includes standard, reconfigurable connections of the pressure vessel to the modular chassis system. A standardized, modular propulsion system includes a magnetic clutch, and a magnetic sleeve used to power the URV on or off.

Abstract: A modular system for building underwater robotic vehicles (URVs), including a pressure vessel system, modular chassis elements, a propulsion system and compatible buoyancy modules. The pressure vessel system uses standardized, interchangeable modules to allow for ease of modification of the URV and accommodation of different internal and external components such as sensors and computer systems. The system also includes standard, reconfigurable connections of the pressure vessel to the modular chassis system. A standardized, modular propulsion system includes a magnetic clutch, and a magnetic sleeve used to power the URV on or off.

Abstract: Methods, systems, apparatuses, and computer-readable mediums are described for techniques for supporting telematics-enhanced emergency calls from mobile phones. In some aspects, a method for wireless communication may include establishing, by a user equipment (UE), a personal telematics-enhanced emergency call to a public safety answering point (PSAP), wherein the UE is capable of transmitting telematics data. The method may also include transmitting information that distinguishes the personal telematics-enhanced emergency call from other types of emergency calls.

Abstract: A system for conducting an assay comprises a power source (16), a controller (13) for controlling the assay and a plurality of assay units (14) operatively connected to one another such that the controller can communicate with the assay units and the system is capable of conducting the assay. An assay device comprises a substantially circular body (24) having a plurality of chambers in fluid connection such that fluid can pass between said chambers and a central hub (200) having a sample inlet (202) disposed therein for receiving a sample.

Abstract: Described is a process for producing a mutant bacterium which exhibits improved survival and/or growth under a selected growth condition, the process comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter capable of increasing transcription of a gene at or near its insertion site; (b) growing bacteria from the mutant pool under the selected growth condition and under one or more reference conditions to produce two or more test cultures; and (c) comparing the distribution of TnA insertions between test cultures to identify a first class of genes which are disadvantageous for growth and/or survival under the selected growth condition and a second class of genes which are advantageous for growth and/or survival under the selected growth condition.

Abstract: A system for a vehicle includes a misfire indication module, a valve control module, and a fault indication module. The misfire indication module selectively indicates that misfire occurred within a cylinder of an engine. The valve control module controls lifting of a valve of a cylinder of the engine and, in response to the misfire indication module indicating that misfire occurred within the cylinder, transitions lifting of the valve from one of a low lift state and a high lift state to the other one of the low lift state and the high lift state. The fault indication module selectively indicates that a fault is present in a variable valve lift (VVL) mechanism of the cylinder based on whether the misfire indication module indicates that misfire occurred within the cylinder after the transition to the other one of the low lift state and the high lift state.

Abstract: A system for a vehicle includes a knock indication module, a valve control module, and a fault indication module. The knock indication module selectively indicates that knock occurred within a cylinder of an engine. The valve control module controls lifting of a valve of a cylinder of the engine and, in response to the knock indication module indicating that knock occurred within the cylinder, transitions lifting of the valve from one of a low lift state and a high lift state to the other one of the low lift state and the high lift state. The fault indication module selectively indicates that a fault is present in a variable valve lift (VVL) mechanism of the cylinder based on whether the knock indication module indicates that knock occurred within the cylinder after the transition to the other one of the low lift state and the high lift state.

Abstract: A method, an apparatus, and a computer program product for wireless communication are provided in connection with improving QoE in RAN congestion. In one example, a communications device is equipped to indicate a quality control indicator (QCI) for each of a plurality of applications that communicate with a RAN over a bearer, receive information regarding modification of the bearer or additional bearers based on the QCIs, and modify the bearer or additional bearers according to the information to achieve a desired QoE for at least one of the plurality of applications. In another example, a RAN is equipped to receive a QCI for each of a plurality of applications related to a bearer from a UE, and modify the bearer or adding additional bearers for communicating with the UE based on the QCI for each of the plurality of applications to improve QoE at the UE.

Abstract: A modular system for building underwater robotic vehicles (URVs), including a pressure vessel system, modular chassis elements, a propulsion system and compatible buoyancy modules. The pressure vessel system uses standardized, interchangeable modules to allow for ease of modification of the URV and accommodation of different internal and external components such as sensors and computer systems. The system also includes standard, reconfigurable connections of the pressure vessel to the modular chassis system. A standardized, modular propulsion system includes a magnetic clutch, and a magnetic sleeve used to power the URV on or off.

Abstract: A Home Public Land Mobile Network (HPLMN) or home network immediately accepts a registration requests from “eCall-only” subscribers attempting to register with any Visitor Public Land Mobile Networks (VPLMNs) or roaming network. This avoids unnecessary delays in the establishment of an eCall (emergency call) that might be otherwise introduced by the home network's roaming registration management policy (RRMP) that otherwise would only permit registrations (and subsequent call servicing) by preferred roaming VPLMN partners and automatically reject registrations from non-preferred roaming networks (which may hinder or prevent timely completion of an eCall).

Abstract: Systems, methods, and computer-readable media for determining the Internet search popularity of an entity are provided. Embodiments of the present invention include receiving a group of Internet search records and assigning a popularity ranking based on the number of times an entity descriptor associated with an entity occurs within the group of Internet search records created over a designated time period. An entity descriptor is one or more terms commonly used to identify an entity. The trend in an entity's popularity rank may also be calculated. An entity's popularity rank and trend in popularity rank may be presented in a graph or in a list.

Abstract: Disclosed is a method for identifying an essential gene which serves as an antibiotic target in a bacterium, the method comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter such that transposon insertion into bacterial DNA increases the transcription of a gene at or near the insertion site; (b) growing bacteria from the mutant pool in the presence of different amounts of said antibiotic to produce two or more test cultures; and (c) comparing the distribution of TnA insertions between test cultures to identify a putative essential gene serving as a target of said antibiotic in said bacterium.