Patents by Inventor Igor Nikolaev

Igor Nikolaev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • COMPOSITIONS AND METHODS FOR ENHANCED PROTEIN PRODUCTION IN FILAMENTOUS FUNGAL CELLS
    Publication number: 20230174998
    Abstract: The present disclosure is generally related to mutant and genetically modified filamentous fungal cells and methods thereof for use in the production of proteins of interest. More particularly, as described herein, the mutant and/or modified fungal cells (strains) of the disclosure are well-suited for use in industrial scale fermentation processes for the enhanced expression/production of proteins of interest in the absence and/or in the presence of an inducing substrate.
    Type: Application
    Filed: April 9, 2021
    Publication date: June 8, 2023
    Inventors: Yun Luo, Igor Nikolaev, Robert James Pratt, II
  • FUSION POLYPEPTIDES
    Publication number: 20230159937
    Abstract: Provided herein, inter alia, are fusion DNA constructs comprising improved protease recognition sequences for expressing and purifying one or more polypeptides of interest as well as methods for producing one or more polypeptides of interest in a recombinant host cell.
    Type: Application
    Filed: March 9, 2020
    Publication date: May 25, 2023
    Inventors: Jimmy CHAN, David A. ESTELL, Amy Deming LIU, Michael C. MILLER, Igor NIKOLAEV
  • THERMOSTABLE PHYTASE VARIANTS
    Publication number: 20230009832
    Abstract: Provided herein, inter alia, are engineered phytase polypeptides and fragments thereof with improved thermotolerance as well as methods for producing and using the same for enhancing animal performance on one or more metrics.
    Type: Application
    Filed: November 20, 2020
    Publication date: January 12, 2023
    Inventors: Lilia Maria Babe, Trine Christensen, Svend Haaning, Rie Mejldal, Igor Nikolaev, Sina Pricelius, Jens Frisbaek Sørensen, Robin Anton Sorg
  • THERMOSTABLE GLUCOAMYLASE AND METHODS OF USE, THEREOF
    Publication number: 20230002749
    Abstract: Described are polypeptides having glucoamylase activity, compositions comprising such polypeptides, and methods of using such polypeptides and compositions.
    Type: Application
    Filed: November 9, 2017
    Publication date: January 5, 2023
    Inventors: Jacob Flyvholm Cramer, Jing Ge, Marc Anton Bernhard Kolkman, Igor Nikolaev, Jayarama K. Shetty, Zhongmei Tang, Wilhelmus Antonius Van Der Kleij, Zhiyong Xie, Bo Zhang, Zhengzheng Zou
  • ENGINEERED ANTIBODIES
    Publication number: 20220169706
    Abstract: Disclosed herein, inter alia, are cleavage-resistant antibodies and antibody-producing host cells as well as methods for making, using, and improving secretion of the same.
    Type: Application
    Filed: March 30, 2020
    Publication date: June 2, 2022
    Inventors: Jimmy CHAN, David A. ESTELL, Jeffrey Wayne MUNOS, Michael C. MILLER, Igor NIKOLAEV
  • METHOD FOR INCREASING THE PRODUCTION OF ETHANOL FROM CORN FIBER IN A STARCH HYDROLYSIS PROCESS
    Publication number: 20200095614
    Abstract: Described are compositions and methods relating the use of ?-glucosidase to increase the production of ethanol from a corn fiber product in a starch hydrolysis process.
    Type: Application
    Filed: December 15, 2017
    Publication date: March 26, 2020
    Inventors: Jeffrey V. Miller, Bradley Roger Kelemen, Pim Van Der Kley, Igor Nikolaev, Su Yin Marina Chow, Monica Tse, Jing Ge, Zhongmei Tang, Kirstin Yasuko Nose Crotty
  • Polypeptide having xylanase activity
    Patent number: 10550375
    Abstract: The present invention relates to an isolated polypeptide having xylanase activity, selected from the group consisting of: a) a polypeptide comprising an amino acid sequence having at least 87% identity with SEQ ID NO: 1; b) a polypeptide encoded by a polynucleotide having at least 87% identity with SEQ ID NO:2; or c) a fragment of a polypeptide of a) or b) which fragment has xylanase activity.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: February 4, 2020
    Assignee: DuPont Nutrition Biosciences ApS
    Inventors: Rikke Hoeegh Lorentsen, Susan Arent Lund, Svend Haaning, Igor Nikolaev, Sharief Barends, Jan Hendrick A Van Tuijl, Bart Koops
  • Variant enzymes
    Patent number: 10479983
    Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
    Type: Grant
    Filed: July 24, 2014
    Date of Patent: November 19, 2019
    Assignee: DANISCO US INC
    Inventors: Nicholai Douglas, Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Suzanne E. Lantz, Jonathan K. Lassila, Igor Nikolaev, Daniel Esteban Torres-Pazmino, Wilhelmus Van Der Kley, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
  • VARIANT ENZYMES
    Publication number: 20190169587
    Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
    Type: Application
    Filed: November 9, 2018
    Publication date: June 6, 2019
    Inventors: MARIA FOUKARAKI, RONALDUS WILHELMUS JOANNES HOMMES, THIJS KAPER, BRADLEY R. KELEMEN, SLAVKO KRALJ, SUZANNE E. LANTZ, IGOR NIKOLAEV, WILHELMUS ANTONIUS HENDRICUS VAN DER KLEIJ, JOHANNES FRANCISCUS THOMAS VAN LIESHOUT, SANDER VAN STIGT THANS
  • Composition and methods comprising a xylanase enzyme variant
    Patent number: 10253304
    Abstract: The present invention provides hemicellulytic enzyme variants. Specifically, the present invention provides xylanase variants having one or more modifications as compared to a parent xylanase enzyme resulting in at least one improved property. In addition, the present invention provides compositions comprising a xylanase variant of the invention. The present invention also provides methods of degrading hemicellulotic material, including arabino-xylan using compositions comprising a xylanase variant of the invention.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: April 9, 2019
    Assignee: DUPONT NUTRITION BIOSIENCES APS
    Inventors: Rikke Hoeegh Lorentsen, Susan Arent Lund, Jacob F. Cramer, Igor Nikolaev, Wilhelmus Van Der Kleij
  • Variant enzymes
    Patent number: 10167460
    Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
    Type: Grant
    Filed: July 24, 2014
    Date of Patent: January 1, 2019
    Assignee: DANISCO US INC
    Inventors: Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Suzanne E. Lantz, Igor Nikolaev, Wilhelmus Van Der Kley, Johan Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
  • GH10 family xylanase
    Patent number: 10165787
    Abstract: A GH10 xylanase variant is disclosed herein. This GH10 xylanase variant has increased thermostability compared with a parent GH10 xylanase enzyme comprising at least 90% sequence identity with the amino acid sequence of SEQ ID No. 1 wherein the variant has been modified at two or more of the following positions 7, 33, 79, 217 and 298 corresponding to the amino acid numbering of SEQ ID No. 1.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: January 1, 2019
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Rikke Hoeegh Lorentsen, Susan Arent Lund, Igor Nikolaev, Jan Hendrik A. Van Tuijl, Bert Koops
  • Variant Enzymes
    Patent number: 10081802
    Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
    Type: Grant
    Filed: July 24, 2014
    Date of Patent: September 25, 2018
    Assignee: DANISCO US INC.
    Inventors: Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Suzanne E. Lantz, Igor Nikolaev, Wilhelmus Van Der Kley, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
  • CELLULASE COMPOSITIONS AND METHODS OF USING THE SAME FOR IMPROVED CONVERSION OF LIGNOCELLULOSIC BIOMASS INTO FERMENTABLE SUGARS
    Publication number: 20180119125
    Abstract: The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having ?-glucosidase activity, methods for hydrolyzing biomass material, and methods for improving the stability and saccharification efficacy of a composition comprising such ?-glucosidase polypeptides and/or activity.
    Type: Application
    Filed: February 23, 2017
    Publication date: May 3, 2018
    Inventors: THIJS KAPER, IGOR NIKOLAEV, SUZANNE E. LANTZ, Meredith K. Fujdala, Megan Y. Hsi
  • Variants of cellobiohydrolases
    Patent number: 9938551
    Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Ce17A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
    Type: Grant
    Filed: December 10, 2013
    Date of Patent: April 10, 2018
    Assignee: DANISCO US INC
    Inventors: Richard R. Bott, Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Igor Nikolaev, Mats Sandgren, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
  • VARIANTS HAVING GLUCOAMYLASE ACTIVITY
    Publication number: 20170306309
    Abstract: The present invention relates to variants having glucoamylase activity with improved properties and to compositions comprising these variants suitable for use for example in the production of a food, beverage (e.g. beer), feed, biochemical, or biofuel. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.
    Type: Application
    Filed: April 12, 2017
    Publication date: October 26, 2017
    Applicant: DuPont Nutrition Biosciences APS
    Inventors: Jacob Flyvholm CRAMER, Peter Edvard DEGN, Igor NIKOLAEV, Viktor ALEKSEYEV, Casper Willem VROEMEN, Daniel TORRES PAZMINO, Neville Marshall FISH
  • FUNGAL HOST STRAINS , DNA CONSTRUCTS , AND METHODS OF USE
    Publication number: 20170260559
    Abstract: Provided are fungal host strains and recombinant DNA constructs for creation and use thereof, wherein the fungal host strains are particularly stable and useful for expressing proteins, enzymes, variants and other substances of interest in a reliable or less variable fashion.
    Type: Application
    Filed: December 1, 2015
    Publication date: September 14, 2017
    Applicant: DANISCO US INC.
    Inventors: Mark ARENTSHORST, Sharief BARENDS, Paulien KRUITHOF, Igor NIKOLAEV, Jean Paul OUEDRAOGO, Arthur F.J. RAM
  • Polypeptides having glucoamylase activity and method of producing the same
    Patent number: 9677058
    Abstract: The present invention relates to polypeptides having glucoamylase activity with improved properties and to compositions comprising these polypeptides suitable for use in the production of a food, beverage (e.g. beer), feed, or biofuel. Also described is an improved and cost-effective process for isolating glucoamylases suitable for large scale protein purification procedures. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: June 13, 2017
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Jacob Flyvholm Cramer, Neville Marshall Fish, Peter Edvard Degn, Igor Nikolaev, Paulien Kruithof, Piet Van Solingen, Sander Van Stigt Thans, Suzy Breneman, Jayarama K. Shetty, Sung Ho Lee
  • BETA-GLUCOSIDASE I VARIANTS WITH IMPROVED PROPERTIES
    Publication number: 20170145396
    Abstract: The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.
    Type: Application
    Filed: August 29, 2016
    Publication date: May 25, 2017
    Applicant: DANISCO US INC.
    Inventors: Richard R. BOTT, Thijs KAPER, Bradley R. KELEMEN, Frits GOEDEGEBUUR, Ronaldus Wilhelmus Joannes HOMMES, Slavko KRALJ, Paulien KRUITHOF, Igor NIKOLAEV, Wilhelmus Antonious Hendricus VAN DER KLEY, Johannes Franciscus Thomas VAN LIESHOUT, Sander VAN STIGT THANS, Gudrun VOGTENTANZ, Mats SANDGREN
  • PROTEIN
    Publication number: 20170009220
    Abstract: The present invention relates to an isolated polypeptide having xylanase activity, selected from the group consisting of: a) a polypeptide comprising an amino acid sequence having at least 87% identity with SEQ ID NO: 1; b) a polypeptide encoded by a polynucleotide having at least 87% identity with SEQ ID NO:2; or c) a fragment of a polypeptide of a) or b) which fragment has xylanase activity.
    Type: Application
    Filed: January 30, 2015
    Publication date: January 12, 2017
    Applicant: DuPont Nutrition Biosciences ApS
    Inventors: Rikke Hoeegh Lorentsen, Susan Arent Lund, Svend Haaning, Igor Nikolaev, Sharief Barends, Jan Hendrick A Van Tuijl, Bert Koops