Patents by Inventor Igor Nikolaev
Igor Nikolaev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9523087Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.Type: GrantFiled: February 25, 2014Date of Patent: December 20, 2016Assignee: DANISCO US INCInventors: Wolfgang Aehle, Richard R. Bott, Benjamin S. Bower, Jonathan Caspi, David A. Estell, Frits Goedegebuur, Ronaldus Wilhelmus Joannes Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Johannes Franciscus Thomas Van Lieshout, Igor Nikolaev, Sander Van Stigt Thans, Louise Wallace, Gudrun Vogtentanz, Mats Sandgren
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Publication number: 20160345609Abstract: The present inventions relates to a modified GH10 xylanase enzyme or a fragment thereof having xylanase activity wherein said modified GH10 xylanase or fragment thereof has increased thermostability compared with a parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at, at least, two of the following positions 7, 33, 79, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). In particular, the modified xylanase enzyme according to the present invention has the following modifications: N7D; T33V; K79Y, V, F, I, L or M (preferably K79Y, F or V, more preferably Y); A217Q, E, P, D or M (preferably A217Q, E or P, more preferably Q); and T298Y, F or W (preferably Y or F, more preferably Y).Type: ApplicationFiled: January 30, 2015Publication date: December 1, 2016Inventors: Rikke Hoeegh Lorentsen, Susan Arent Lund, Igor Nikolaev, Jan Hendrik A. Van Tuijl, Bert Koops
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Publication number: 20160343077Abstract: The Probabilistic Analysis Trading Platform Apparatuses, Methods and Systems (“PATP”) transforms model training request and security analysis request inputs via PATP components into model parameters data, model training response, order request, and security analysis response outputs. A security analysis request associated with a security may be obtained via security analysis component. Model parameters of a model associated with the security may be retrieved. Modified nodes of the model for which probabilities have been modified by expert input data associated with the security analysis request may be determined. Dependent nodes for each of the modified nodes may be determined. Probabilities associated with the dependent nodes may be recalculated and an output value associated with a result node of the model may be determined based on the recalculated probabilities. A trading action may be facilitated based on the output value.Type: ApplicationFiled: May 18, 2015Publication date: November 24, 2016Inventors: Dmitry Bisikalo, Igor Nikolaev, Tom Mulligan
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Publication number: 20160340664Abstract: The present invention provides hemicellulytic enzyme variants. Specifically, the present invention provides xylanase variants having one or more modifications as compared to a parent xylanase enzyme resulting in at least one improved property. In addition, the present invention provides compositions comprising a xylanase variant of the invention. The present invention also provides methods of degrading hemicellulotic material, including arabino-xylan using compositions comprising a xylanase variant of the invention.Type: ApplicationFiled: January 30, 2015Publication date: November 24, 2016Inventors: Susan Arent Lund, Jacob F. Cramer, Igor Nikolaev, Pim Van Der Kley
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Publication number: 20160272959Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.Type: ApplicationFiled: December 10, 2013Publication date: September 22, 2016Applicant: Danisco US Inc.Inventors: Richard R. BOTT, Maria FOUKARAKI, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, Igor NIKOLAEV, Mats SANDGREN, Johannes Franciscus Thomas VAN LIESHOUT, SANDER VAN STIGT THANS
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Patent number: 9447400Abstract: The present disclosure is generally directed to enzymes and in particular beta-glucosidase variants. Also described are nucleic acids encoding beta-glucosidase variants, compositions comprising beta-glucosidase variants, methods of using beta-glucosidase variants, and methods of identifying additional useful beta-glucosidase variants.Type: GrantFiled: November 19, 2010Date of Patent: September 20, 2016Assignee: DANISCO US INC.Inventors: Richard R. Bott, Thijs Kaper, Bradley Kelemen, Frits Goedegebuur, Ronaldus Wilhelmus Hommes, Slavko Kralj, Paulien Kruithof, Igor Nikolaev, Wilhelmus Antonious Hendricus Van Der Kley, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans, Gudrun Vogtentanz, Mats Sandgren
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Publication number: 20160201104Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Ce17A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.Type: ApplicationFiled: December 10, 2013Publication date: July 14, 2016Applicant: DANISCO US INC.Inventors: Richard R. BOTT, Maria FOUKARAKI, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, Igor NIKOLAEV, Mats SANDGREN, Johannes Franciscus Thomas VAN LIESHOUT, SANDER VAN STIGT THANS
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Publication number: 20160186155Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.Type: ApplicationFiled: July 24, 2014Publication date: June 30, 2016Inventors: Nicholai DOUGLAS, Maria FOUKARAKI, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, SUZANNE E. LANTZ, Jonathan K. LASSILA, Igor NIKOLAEV, Daniel Esteban TORRES-PAZMINO, Wilhelmus VAN DER KLEY, Johannes Franciscus Thomas VAN LIESHOUT, SANDER VAN STIGT THANS
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Publication number: 20160177281Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.Type: ApplicationFiled: July 24, 2014Publication date: June 23, 2016Inventors: Maria FOUKARAKI, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, SUZANNE E. LANTZ, IGOR NIKOLAEV, Wilhelmus VAN DER KLEY, Johan Franciscus Thomas VAN LIESHOUT, SANDER VAN STIGT THANS
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Publication number: 20160168550Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.Type: ApplicationFiled: July 24, 2014Publication date: June 16, 2016Inventors: Maria FOUKARAKI, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, SUZANNE E. LANTZ, IGOR NIKOLAEV, Wilhelmus VAN DER KLEY, Johannes Franciscus Thomas VAN LIESHOUT, SANDER VAN STIGT THANS
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Publication number: 20160122734Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.Type: ApplicationFiled: December 10, 2013Publication date: May 5, 2016Applicant: DANISCO US INC.Inventors: Richard R. BOTT, Maria FOUKARAKI, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, Igor NIKOLAEV, Mats SANDGREN, Johannes Franciscus Thomas VAN LIESHOUT, SANDER VAN STIGT THANS
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Publication number: 20160122735Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.Type: ApplicationFiled: December 10, 2013Publication date: May 5, 2016Applicant: Danisco US Inc.Inventors: Richard R. BOTT, Maria FOUKARAKI, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, Igor NIKOLAEV, Mats SANDGREN, Johannes Franciscus Thomas VAN LIESHOUT, Sander VAN STIGT THANS
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Publication number: 20150315560Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.Type: ApplicationFiled: December 10, 2013Publication date: November 5, 2015Applicant: Danisco US Inc.Inventors: Richard R. BOTT, Maria FOUKARAKI, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, Igor NIKOLAEV, Mats SANDGREN, Johannes Franciscus Thomas VAN LIESHOUT, SANDER VAN STIGT THANS
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Publication number: 20150315558Abstract: The present invention relates to variants having glucoamylase activity with improved properties and to compositions comprising these variants suitable for use for example in the production of a food, beverage (e.g. beer), feed, biochemical, or biofuel. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.Type: ApplicationFiled: August 21, 2013Publication date: November 5, 2015Applicant: DUPONT NUTRITION BIOSCIENCES APSInventors: Jacob Flyvholm Cramer, Peter Edvard Degn, Igor Nikolaev, Viktor Alekseyev, Casper Willem Vroemen, Daniel Torres Pazmino, Neville Marshall Fish
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Patent number: 9113649Abstract: The present invention relates to amidase enzymes and a feed or food additive comprising the amidase enzyme capable of degrading ochratoxin.Type: GrantFiled: September 6, 2011Date of Patent: August 25, 2015Assignee: DuPont Nutrition Biosciences ApSInventors: Shukun Yu, Charlotte Horsmans Poulsen, Soren Dalsgaard, Huaming Wang, Igor Nikolaev
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Publication number: 20140356922Abstract: The present invention relates to polypeptides having glucoamylase activity with improved properties and to compositions comprising these polypeptides suitable for use in the production of a food, beverage (e.g. beer), feed, or biofuel. Also described is an improved and cost-effective process for isolating glucoamylases suitable for large scale protein purification procedures. Furthermore, different methods and uses related to glucoamylases according to the invention are disclosed, such as in a brewing process.Type: ApplicationFiled: December 20, 2012Publication date: December 4, 2014Inventors: Jacob Flyvholm Cramer, Neville Marshall Fish, Peter Edvard Degn, Igor Nikolaev, Paulien Kruithof, Piet Van Solingen, Sander Van Stigt Thans, Suzy Breneman, Jayarama K. Shetty, Sung Ho Lee
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Publication number: 20140302585Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.Type: ApplicationFiled: February 25, 2014Publication date: October 9, 2014Applicant: DANISCO US INC.Inventors: Wolfgang AEHLE, Richard R. BOTT, Benjamin S. BOWER, Jonathan CASPI, David A. ESTELL, Frits GOEDEGEBUUR, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, Johannes Franciscus Thomas VAN LIESHOUT, Igor NIKOLAEV, Sander VAN STIGT THANS, Louise WALLACE, Gudrun VOGTENTANZ, Mats SANDGREN
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Patent number: 8802398Abstract: A method of oxidizing a saccharide, by contacting the saccharide with an alcohol dehydrogenase (ADH) enzyme selected from a quinone redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide (NAD+) redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide phosphate (NADP+) redox cofactor-dependent ADH, and any combination thereof is described. An oxidized saccharide obtainable by the method and products, in particular food products and paper products, containing the oxidized saccharide, are also described.Type: GrantFiled: October 29, 2010Date of Patent: August 12, 2014Assignee: Dupont Nutrition Biosciences ApsInventors: Jorn Dalgaard Mikkelsen, Karsten Matthias Kragh, Rene Mikkelsen, Patrick Maria Franciscus Derkx, Shukun Yu, Harm Mulder, Igor Nikolaev
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Patent number: 8791232Abstract: An amino acid sequence is described. The amino acid may comprise a signal sequence that is SEQ ID NO. 1a (or a variant or homologue or derivative or fragment thereof) that is expressed as a fusion protein that comprises a protein of interest. The signal sequence directs secretion of the protein of interest. The protein of interest may be a heterologous protein. Secretion of the fusion protein aids purification.Type: GrantFiled: October 29, 2010Date of Patent: July 29, 2014Assignee: Dupont Nutrition Biosciences APSInventors: Patrick Maria Franciscus Derkx, Harm Mulder, Igor Nikolaev
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Publication number: 20140142998Abstract: A computer-implemented method is provided for assigning tasks to resources. The method includes receiving one or more constraints corresponding to each of the tasks and one or more constraints corresponding to each of the resources. The method also includes receiving a value from a user defining at least one of a plurality of variables including: (i) a resource variable representing resources available for allocation to the tasks, (ii) a duration variable representing a time frame for completing the tasks, and iii) a scope variable representing one or more of the plurality of tasks requiring execution. The method further includes applying a first optimization scheme to determine a value for each undefined variable to formulate a task delivery plan and applying a second optimization scheme to determine a task for assignment to a resource.Type: ApplicationFiled: November 19, 2012Publication date: May 22, 2014Applicant: FMR LLCInventors: Keith Kroeger, Igor Nikolaev