Patents by Inventor James Eberwine

James Eberwine has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Compositions, Methods and Kits for Real-Time Nucleic Acid Analysis in Live Cells
    Publication number: 20160168558
    Abstract: The present invention includes compositions, methods and kits for the real-time detection of transcription and translation in live cells, tissues and organisms. The present invention further provides method for the rapid sequencing of nucleic acids without using conventional sequencing techniques or reactions.
    Type: Application
    Filed: December 31, 2015
    Publication date: June 16, 2016
    Inventors: James Eberwine, Ulo Langel, Emelia Eiriksdottir, Tiina Peritz [nee Pajunen], Jai-Yoon Sul, Philip G. Haydon, Junhyong Kim
  • Transcriptome Transfer Produces Cellular Phenotype Conversion
    Publication number: 20160046958
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Application
    Filed: October 12, 2015
    Publication date: February 18, 2016
    Inventors: James Eberwine, Jai-Yoon Sul, Tae Kyung Kim, Vickas Patel
  • Transcriptome transfer produces cellular phenotype conversion
    Patent number: 9157066
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Grant
    Filed: July 19, 2011
    Date of Patent: October 13, 2015
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Jai-Yoon Sul, Tae Kyung Kim, Vickas Patel
  • Transcriptome Transfer Produces Cellular Phenotype Conversion
    Publication number: 20140206059
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Application
    Filed: March 31, 2014
    Publication date: July 24, 2014
    Applicant: The Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Jai-Yoon Sul, Chia-Wen Wu, Fanyi Zeng, Junhyong Kim
  • Methods and compositions for identifying RNA-binding proteins
    Patent number: 8632972
    Abstract: The present invention includes compositions, methods and kits for the identification of a polypeptide that binds to a predetermined RNA sequence. The invention comprises, in part, a photoreactive moiety to aid in identification of such a polypeptide.
    Type: Grant
    Filed: June 20, 2006
    Date of Patent: January 21, 2014
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Ulo Langel, Kalle Kilk, Jennifer Zielinski
  • Transcriptome In Vivo Analysis
    Publication number: 20130273537
    Abstract: The invention provides compositions and methods that permit a hybrid nucleic acid molecule to enter a cell and when specifically activated within the cell, the molecule anneals to endogenous cellular RNA and permits the isolation of the RNA.
    Type: Application
    Filed: March 15, 2013
    Publication date: October 17, 2013
    Inventors: James Eberwine, Ditte Lovatt, Julia Richards, Ivan Dmochowski, Brittani Ruble
  • TRANSCRIPTOME IN VIVO ANALYSIS
    Publication number: 20130267678
    Abstract: Provided are compositions and methods that permit a hybrid nucleic acid-peptide molecule to enter a cell and when specifically activated within the cell, the molecule anneals to endogenous cellular RNA and permits the isolation of the RNA.
    Type: Application
    Filed: June 7, 2011
    Publication date: October 10, 2013
    Applicant: The Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Ditte Lovatt, Julia Richards, Ivan Dmochowski, Brittani Ruble
  • Cytoplasmic BKchannel intron-containing mRNAs contribute to the intrinsic excitability of hippocampal neurons
    Patent number: 8536146
    Abstract: The invention relates to a method of modulating neuronal function by modulating the cytoplasmic level in a neuron of an intron-containing mRNA. The methods are useful in diagnostic, research and therapeutic applications.
    Type: Grant
    Filed: April 25, 2008
    Date of Patent: September 17, 2013
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Jai-Yoon Sul, Thomas J. Bell, Kevin Miyashiro
  • Methods and Compositions for In-Vivo Enzyme Capture
    Publication number: 20130040309
    Abstract: The invention includes compositions, methods and kits for the in vivo identification of an enzyme that binds to a substrate. The invention comprises, in part, a photoreactive moiety to aid in identification of such an enzyme.
    Type: Application
    Filed: September 22, 2010
    Publication date: February 14, 2013
    Inventors: James Eberwine, Ülo Langel, Emelia Eiriksdóttir, Anup Sharma
  • Extranuclear RNA splicing in neuronal dendrites
    Patent number: 8367402
    Abstract: The present invention relates to methods of synaptic network remodeling by means of extranuclear RNA splicing. The present invention also provides methods of extranuclear RNA splicing, and methods of protein translation based on extranuclear RNA splicing.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: February 5, 2013
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Kevin Miyashiro, Jason Glanzer
  • Rapid, Sensitive and Quantitative Methods for Tissue and Cell-Based Proteomics Via Consecutive Additional Quantifiable Extenders
    Publication number: 20120329062
    Abstract: Methods, systems and kits are provided for detecting and quantifying multiple immunogens in a sample via consecutive addition of quantifiable extenders to immunogen bound complexes of immunogen binding agent attached to a DNA containing an RNA polymerase promoter.
    Type: Application
    Filed: August 14, 2012
    Publication date: December 27, 2012
    Applicant: Trustees of the University of Pennsylvania
    Inventor: James Eberwine
  • Rapid, sensitive and quantitative methods for tissue and cell-based proteomics via consecutive addition of quantifiable extenders
    Patent number: 8241858
    Abstract: Methods, systems and kits are provided for detecting and quantifying multiple immunogens in a sample via consecutive addition of quantifiable extenders to immunogen bound complexes of immunogen binding agent attached to a DNA containing an RNA polymerase promoter.
    Type: Grant
    Filed: May 11, 2011
    Date of Patent: August 14, 2012
    Assignee: The Trustees of the University of Pennsylvania
    Inventor: James Eberwine
  • Methods for Transfecting Nucleic Acid Into Live Cells
    Publication number: 20120178167
    Abstract: The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell.
    Type: Application
    Filed: March 21, 2012
    Publication date: July 12, 2012
    Inventors: James Eberwine, Philip G. Haydon, Jai-Yoon Sul, Hajime Takano, Chia-Wen Kitty Wu, Fanyi Zeng
  • Methods for Phototransfecting Nucleic Acids Into Live Cells
    Publication number: 20120135493
    Abstract: The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell.
    Type: Application
    Filed: December 12, 2006
    Publication date: May 31, 2012
    Inventors: James Eberwine, Philip G. Haydon, Jai-Yoon Sul, Hajime Takano, Chia-Wen Kitty Wu, Fanyi Zeng
  • Transcriptome Transfer Produces Cellular Phenotype Conversion
    Publication number: 20120129261
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Application
    Filed: July 19, 2011
    Publication date: May 24, 2012
    Inventors: James Eberwine, Jai-Yoon Sul, Tai Kyung Kim, Vickas Patel
  • Rapid, Sensitive and Quantitative Methods for Tissue and Cell-Based Proteomics Via Consecutive Addition of Quantifiable Extenders
    Publication number: 20110245467
    Abstract: Methods, systems and kits are provided for detecting and quantifying multiple immunogens in a sample via consecutive addition of quantifiable extenders to immunogen bound complexes of immunogen binding agent attached to a DNA containing an RNA polymerase promoter.
    Type: Application
    Filed: May 11, 2011
    Publication date: October 6, 2011
    Applicant: Trustees of the University of Pennsylvania
    Inventor: James Eberwine
  • Rapid, sensitive and quantitative methods for tissue and cell-based proteomics via consecutive addition of quantifiable extenders
    Patent number: 7943321
    Abstract: Methods, systems and kits are provided for detecting and quantifying multiple immunogens in a sample via consecutive addition of quantifiable extenders to immunogen bound complexes of immunogen binding agent attached to a DNA containing an RNA polymerase promoter.
    Type: Grant
    Filed: June 1, 2009
    Date of Patent: May 17, 2011
    Assignee: Trustees of the University of Pennsylvania
    Inventor: James Eberwine
  • Transcriptome Transfer Produces Cellular Phenotype Conversion
    Publication number: 20110033934
    Abstract: The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
    Type: Application
    Filed: April 6, 2010
    Publication date: February 10, 2011
    Inventors: James Eberwine, Jai-Yoon Sul, Chia-Wen Wu, Fanyi Zeng, Junhyong Kim
  • Extranuclear RNA Splicing in Neuronal Dendrites
    Publication number: 20100159525
    Abstract: The present invention relates to methods of synaptic network remodeling by means of extranuclear RNA splicing. The present invention also provides methods of extranuclear RNA splicing, and methods of protein translation based on extranuclear RNA splicing.
    Type: Application
    Filed: December 1, 2009
    Publication date: June 24, 2010
    Inventors: James Eberwine, Kevin Miyashiro, Jason Glanzer
  • Rapid, Sensitive and Quantitative Methods for Tissue and Cell-Based Proteomics via Consecutive Addition of Quantifiable Extenders
    Publication number: 20090325174
    Abstract: Methods, systems and kits are provided for detecting and quantifying multiple immunogens in a sample via consecutive addition of quantifiable extenders to immunogen bound complexes of immunogen binding agent attached to a DNA containing an RNA polymerase promoter.
    Type: Application
    Filed: June 1, 2009
    Publication date: December 31, 2009
    Inventor: James Eberwine