Patents by Inventor James Eberwine

James Eberwine has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of detecting protein by immuno RNA
    Patent number: 5922553
    Abstract: A method for the detection of a selected protein is provided wherein mRNA levels are correlated with the presence of protein using the technique referred to herein as immuno-aRNA.
    Type: Grant
    Filed: November 17, 1997
    Date of Patent: July 13, 1999
    Assignee: Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Lori Rodgers
  • Methods for profiling mRNA expression in neurites
    Patent number: 5723290
    Abstract: A method of identifying neurite cDNA clones by determining and comparing mRNA expression in selected neurites is provided. cDNA clones identified by this method are also provided. In addition, methods of profiling mRNA expression and diagnosing and treating conditions associated with a pattern of mRNA expression by determining an mRNA expression profile are provided.
    Type: Grant
    Filed: November 3, 1994
    Date of Patent: March 3, 1998
    Assignee: Trustees of the University of Pennsylvania
    Inventors: James Eberwine, Marc Dichter, Kevin Miyashiro
  • Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics
    Patent number: 5514545
    Abstract: A method is provided for characterizing cells at the molecular level by amplifying RNA from selected single cells by microinjecting primer, nucleotides and enzyme into acutely dissociated cells to produce amplified antisense RNA; reamplifying the amplified antisense RNA (aRNA) produced by using random hexanucleotides to prime cDNA synthesis from aRNA, and then detecting messages in the amplified RNA. The invention is useful for characterization of cell identity or physiological state.
    Type: Grant
    Filed: July 26, 1993
    Date of Patent: May 7, 1996
    Assignee: Trustees of the University of Pennsylvania
    Inventor: James Eberwine
  • In situ transcription in cells and tissues
    Patent number: 5168038
    Abstract: In situ transcription is provided by hybridizing cells or tissue with a primer, and extending the primer bound to any template mRNA or DNA with transverse transcriptase or DNA polymerase in the presence of labeled nucleotides, which allows for detection of cells containing the template. The resulting cDNA may be eluted and used in a polymerase chain reaction for isolation of the DNA, and/or the cDNA electrophoresed, which may provide for information concerning the sequence, or the like.
    Type: Grant
    Filed: March 18, 1991
    Date of Patent: December 1, 1992
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Laurence Tecott, Jack D. Barchas, James Eberwine
  • In situ transcription in cells and tissues
    Patent number: 5021335
    Abstract: In situ transcription is provided by hybridizing cells or tissue with a primer, and extending the primer bound to any template mRNA or DNA with reverse transcriptase or DNA polymerase in the presence of labeled nucleotides, which allows for detection of cells containing the template. The resulting cDNA may be eluted and used in a polymerase chain reaction for isolation of the DNA, and/or the cDNA electrophoresed, which may provide for information concerning the sequence, or the like.
    Type: Grant
    Filed: June 17, 1988
    Date of Patent: June 4, 1991
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Laurence Tecott, Jack D. Barchas, James Eberwine