Abstract: A method facilitates assembling a gas turbine engine with a flameholder. The method comprises coupling at least one turning vane between a radially outer casing and a radially inner casing to form a flameholder, forming at least two slots that extend substantially radially through the outer and inner casings, coupling at least one fuel injector to the flameholder, and coupling the flameholder within the augmenter.

Abstract: A fuel shield is configured for use in the afterburner of a turbofan aircraft engine. The shield includes wings obliquely joined together at a nose to conform with the leading edge region of a flameholder vane. A hood is joined to the wings and extends obliquely therefrom to conform with a supporting outer shell of the flameholder.

Abstract: A method for pre-emptively reducing the production of water in an oil and gas well is provided. In one embodiment, a drill-in fluid having a relative permeability modifier (RPM) capable of impeding the production of water is introduced into the wellbore during drilling operations. In a second embodiment, a completion fluid having a relative permeability modifier (RPM) capable of impeding the production of water is introduced into the wellbore during completion operations. The drill-in fluid or completion fluid may further include an organosilicon compound to increase flow resistance and extend the effective RPM permeability application range.

Abstract: A method is disclosed for preparing carrier wafers for semiconductor device manufacture. The method includes the steps of sorting a plurality of standard carrier wafer blanks into batches by thickness to define a batch of starting carrier wafers that are within a predetermined tolerance of one another, reducing the thickness of the sorted carrier wafers to within 10 microns of a final target thickness, and polishing the sorted carrier wafers to the final target thickness. The polished carrier wafers are mounted to device precursor wafers having at least one semiconductor epitaxial layer on a substrate by joining one surface of a carrier wafer to the epitaxial layer on a substrate. The thickness of the device precursor wafer is then reduced by removing material from the device precursor substrate opposite the joined epitaxial layer.

Abstract: A method facilitates assembling a gas turbine engine with a flameholder. The method comprises coupling at least one turning vane between a radially outer casing and a radially inner casing to form a flameholder, forming at least two slots that extend substantially radially through the outer and inner casings, coupling at least one fuel injector to the flameholder, and coupling the flameholder within the augmenter.

Abstract: The invention provides for polynucleotides and vectors comprising at least two tag sequences. In particular, preferred vectors are viral vectors. The invention also provides for polynucleotides and vectors comprising a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for polynucleotides and vectors wherein a gene of interest is fused in frame to at least two tag sequences, for example, a streptavidin binding peptide sequence and a calmodulin binding peptide sequence. The invention also provides for methods of using the polynucleotides and vectors of the invention for detecting and/isolating protein complexes or identifying a binding partner for a protein of interest.

Abstract: The invention provides improved methods of introducing site-directed mutations into circular DNA molecules of interest by means of mutagenic primer pairs. The mutagenic primer pairs are also selected so as to be either completely complementary or partially complementary to each other, wherein the mutation site (or sites) is located within the region of complementarity. A mutagenic primer pair is annealed to opposite strands of a circular DNA molecule containing the DNA sequence to be mutagenized. After annealing, first and second mutagenized DNA strands, each incorporating a member of the mutagenic oligonucleotide primer pair is synthesized by a linear cyclic amplification reaction. After the linear cyclic amplification mediated synthesis step is completed, the reaction mixture is treated with a selection enzyme that digests the parental template strands. After the digesting step, a double-stranded circular DNA intermediate is formed.

Abstract: The invention provides improved methods of introducing site-directed mutations into circular DNA molecules of interest by means of mutagenic primer pairs. The mutagenic primer pairs are also selected so as to be either completely complementary or partially complementary to each other, wherein the mutation site (or sites) is located within the region of complementarity. A mutagenic primer pair is annealed to opposite strands of a circular DNA molecule containing the DNA sequence to be mutagenized. After annealing, first and second mutagenized DNA strands, each incorporating a member of the mutagenic oligonucleotide primer pair is synthesized by a linear cyclic amplification reaction. After the linear cyclic amplification mediated synthesis step is completed, the reaction mixture is treated with a selection enzyme that digests the parental template strands. After the digesting step, a double-stranded circular DNA intermediate is formed.

Abstract: The invention relates to acyclic chain terminator nucleotide analogs. More particularly, the invention relates to phosphonomethoxyethyl nucleotide analogs and detectably labeled versions thereof, especially fluorescently labeled versions thereof. The invention further relates to the use of chain terminating phosphonomethoxyethyl nucleotide analogs in methods of synthesizing a polynucleotide, labeling a polynucleotide, determining polynucleotide sequence information, and kits therefor.

Abstract: The invention provides improved methods of introducing site-directed mutations into circular DNA molecules of interest by means of mutagenic primer pairs. The mutagenic primer pairs are also selected so as to be either completely complementary or partially complementary to each other, wherein the mutation site (or sites) is located within the region of complementarity. A mutagenic primer pair is annealed to opposite strands of a circular DNA molecule containing the DNA sequence to be mutagenized. After annealing, first and second mutagenized DNA strands, each incorporating a member of the mutagenic oligonucleotide primer pair is synthesized by a linear cyclic amplification reaction. After the linear cyclic amplification mediated synthesis step is completed, the reaction mixture is treated with a selection enzyme that digests the parental template strands. After the digesting step, a double-stranded circular DNA intermediate is formed.

Abstract: An improved poult box for transportation of newly hatched turkey poults. The box has an open top, a bottom joined by opposing sidewalls and at least one box divider. Positioned on the box divider is a removable poult feed tray which rests over the divider and presents feed at approximately eye level of turkey poults.

Abstract: The invention provides improved methods of introducing site-directed mutations into circular DNA molecules of interest by means of mutagenic primer pairs. The mutagenic primer pairs are also selected so as to be either completely complementary or partially complementary to each other, wherein the mutation site (or sites) is located within the region of complementarity. A mutagenic primer pair is annealed to opposite strands of a circular DNA molecule containing the DNA sequence to be mutagenized. After annealing, first and second mutagenized DNA strands, each incorporating a member of the mutagenic oligonucleotide primer pair is synthesized by a linear cyclic amplification reaction. After the linear cyclic amplification mediated synthesis step is completed, the reaction mixture is treated with a selection enzyme that digests the parental template strands. After the digesting step, a double-stranded circular DNA intermediate is formed.

Abstract: The invention relates to fluorescent dyes. More particularly, the invention relates to fluorescent cyanine dyes, and especially to water soluble fluorescent cyanine dyes that contain additional sites for attachment to biomolecules. The invention provides a group of novel, water soluble fluorescent cyanine dyes that have distinct fluorescence characteristics that permit their use in any assay or method suited to water soluble fluorescent dyes, and especially to assays requiring a plurality of distinguishable fluorescent markers. The invention further relates to nucleotides, nucleosides, polynucleotides and polypeptides labeled with novel fluorescent cyanine dyes according to the invention, and methods of using them.

Abstract: The present invention relates to a dual-labeled nucleotide comprising a fluorescent label and a quencher of that fluorescent label, wherein the quencher is attached to a phosphate moiety that is cleaved off when the nucleotide is enzymatically incorporated into a polynucleotide. The invention further relates to methods of labeling a polynucleotide molecule, as well as methods for identifying one or more residues of a polynucleotide using the dual-labeled nucleotide analogs of the present invention and kits comprising dual-labeled nucleotide analogs according to the invention.

Abstract: An improved poult box for transportation of newly hatched turkey poults. The box has an open top, a bottom joined by opposing sidewalls and at least one box divider. Positioned on the box divider is a removable poult feed tray which rests over the divider and presents feed at approximately eye level of turkey poults.

Abstract: An assembly, composed of long foamed tubing, with a soft inner surface and a smooth outer surface, so configured that it covers and minimizes the problem of snare points on equipment in contact with a protective covering tarpaulin. Protective tarpaulin are generally made of fabric or synthetic sheets. They can be made waterproof by impregnating them with various water proofing agents as well as being coated with a variety of natural or synthetic coatings such as acrylic or synthetic rubberized latices. Synthetic tarpaulins can be reinforced with a fiber matrics, if needed and used for example to cover pleasure boats, construction equipment, flatbed trucks, construction sites, airplanes, cars and trucks, where one wishes to protect from environmental exposure. Yet in spite of these intentions, these sheets exhibit significant defects in that they can be readily ripped and torn on the sharp and protruding edges of the equipment that they are intended to protect.

Abstract: A self-adhesive prosthetic make-up and a method of using the same is disclosed. Prosthetic make-up is applied to a person's body to create an illusion or for enhancing a costume. The disclosed prosthetic make-up comprises a matrix of thermosetting polymer. The top surface of the matrix may be ornamented by way of features including structure and color to represent an anomaly on or embedded in the skin of the user. Among other anomalies, the disclosed prosthetic make-up may be used to create the illusion of a laceration, a sutured wound, a bullet wound, an eye, bite marks, or a circuit board implanted into the skin.

Abstract: The invention relates to fluorescent dyes. More particularly, the invention relates to fluorescent cyanine dyes, and especially to water soluble fluorescent cyanine dyes that contain additional sites for attachment to biomolecules. The invention provides a group of novel, water soluble fluorescent cyanine dyes that have distinct fluorescence characteristics that permit their use in any assay or method suited to water soluble fluorescent dyes, and especially to assays requiring a plurality of distinguishable fluorescent markers. The invention further relates to nucleotides, nucleosides, polynucleotides and polypeptides labeled with novel fluorescent cyanine dyes according to the invention, and methods of using them.

Abstract: The invention provides improved methods of introducing site-directed mutations into circular DNA molecules of interest by means of mutagenic primer pairs. The mutagenic primer pairs are also selected so as to be either completely complementary or partially complementary to each other, wherein the mutation site (or sites) is located within the region of complementarity. A mutagenic primer pair is annealed to opposite strands of a circular DNA molecule containing the DNA sequence to be mutagenized. After annealing, first, and second mutagenized DNA strands, each incorporating a member of the mutagenic oligonucleotide primer pair is synthesized by a linear cyclic amplification reaction. After the linear cyclic amplification mediated synthesis step is completed, the reaction mixture is treated with a selection enzyme that digests the parental template strands. After the digesting step, a double-stranded circular DNA intermediate is formed.