Abstract: The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.

Abstract: A tipping device for reorienting vertical containers in a horizontal orientation which includes, a lift structured to lift a container, a transfer assembly having two transfer members and a gap there between. The transfer members each have a generally convex portion and an elongated, generally horizontal portion. the generally convex portion is disposed at an elevation that is lower than the generally horizontal portion. The lift slides a container over the generally convex portion onto the generally horizontal portion causing the orientation of the container to change from vertical to generally horizontal.

Abstract: A control system for controlling a plurality of electrical devices (6) the system including a central controller (2) for controlling functions of the devices, a network (4) coupled between the central controller (2) and the devices, the network including a plurality of device drivers (10) for providing drive signals to the electrical devices, the number of drivers being less than the number of devices characterized in that the network includes intelligent devices (8) which enable communication through the network from the central controller (2) to the devices (6).

Abstract: Pharmaceutical compositions in topical or parenteral form containing specific organic mono- or dinitrates, some of them novel compounds, are effective in treating male impotence and erectile dysfunction through topical or intracavernosal administration to the penis. Methods of treatment utilizing the mono- or dinitrate-containing compositions are also disclosed.

Abstract: A method of treating a patient suffering from a condition requiring vasodilator therapy, comprising long term, continuous adminstration of an organic nitrite to the patient in a dosage form capable of delivering a sufficient therapeutic amount of nitrite to the bloodstream of the patient thereby providing effective vasodilator therapy for at least 24 hours without the development of tolerance in the patient. The method of the invention is useful in treating conditions such as angina, particularly chronic, stable angina pectoris, ischemic diseases and congestive heart failure, and for controlling hypertension and/or impotence in male patients.

Abstract: The present invention provides a novel method of vasodilator therapy for treating a patient suffering from a condition, comprising long term, continuous administration of an organic nitrite to a patient suffering from the condition in a dosage form capable of delivering a sufficient therapeutic amount of nitrite to the blood stream of the patent thereby providing effective vasodilator therapy for at least 24 hours without development of tolerance in the patient. The method of the invention is useful in treating conditions such as, for example, angina, particularly chronic, stable angina pectoris, ischemic diseases, congestive heart failure, for controlling hypertension and/or impotence in male patients.Any conventional drug delivery system can be employed in carrying out the method of the invention. The drug delivery system can take virtually as many different forms as there are dosage forms available for delivery of nitrite to a patient.

Abstract: A pad configuration for surface mounted components having contact pads along the periphery thereof includes a plurality of interior pads distributed in a grid with the period of interior pads being three quarters of the period of the peripheral pads multiplied by an integer greater than 1.

Abstract: Novel 1,3-dihydrospiro[isobenzofuran-1,4'-piperidine]s and methods of preparing the same are disclosed. These compounds are useful as analgetic, anticonvulsant and antidepressant agents.

Abstract: Novel 3-anilino-2,4-diazabicyclo[3.2.1]octenes, physiologically tolerable acid addition salts thereof and method of preparing same are described. These compounds are useful as tranquilizers, diuretics and antihypertensives.