Patents by Inventor Jon A. Wolff

Jon A. Wolff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Gene expression with covalently modified polynucleotides
    Patent number: 7049142
    Abstract: A process and compound wherein nucleic acids can be modified with a host of molecules and maintain their ability to be expressed. A modifying chemical attachment of polyions to polynucleotides can be used to facilitate the change of tertiary structure of the nucleic acid and in some cases condensation of nucleic acids into smaller, charged particles useful in delivering the nucleic acid to a cell.
    Type: Grant
    Filed: August 2, 2000
    Date of Patent: May 23, 2006
    Assignee: Mirus Corporation
    Inventors: Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker
  • Process of making a compound by forming a polymer from a template drug
    Patent number: 7049144
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Grant
    Filed: December 5, 2001
    Date of Patent: May 23, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson
  • Process for generating multilayered particles
    Patent number: 7045356
    Abstract: We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
    Type: Grant
    Filed: March 8, 2004
    Date of Patent: May 16, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Vladimir Trubetskoy, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, So Chun Wong, Jason Klein
  • Intravascular delivery of nucleic acid
    Publication number: 20060093584
    Abstract: Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
    Type: Application
    Filed: November 7, 2005
    Publication date: May 4, 2006
    Inventors: Jon Wolff, James Hagstrom, Hans Herweijer, Vladimir Budker, Julia Hegge
  • pH-titratable polyampholytes for delivering polyions to a cell
    Patent number: 7033607
    Abstract: A polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: March 11, 2002
    Date of Patent: April 25, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Vladimir S. Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jon A. Wolff, David B. Rozema, Sean D. Monahan
  • Process of making a compound by forming a polymer from a template drug
    Patent number: 7022525
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Grant
    Filed: January 12, 2004
    Date of Patent: April 4, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Vladimir Trubetskoy, Jon A. Wolff, Paul M. Slattum, Lisa Hanson, Vladimir G. Budker, James E. Hagstrom
  • Reversible modification of membrane interaction
    Patent number: 7019113
    Abstract: An process for the reversible modification of membrane interaction of a compound is described. Modification of membrane interaction can be used to facilitate delivery of molecules to cells in vitro and in vivo. The described modifiers, which are used to reversibly inactivate the membrane active compounds, can also be utilized as cross-linkers or to reverse the charge of a molecule.
    Type: Grant
    Filed: May 23, 2003
    Date of Patent: March 28, 2006
    Assignee: Mirus Bio Corporation
    Inventors: David B. Rozema, Darren Wakefield, Jon A. Wolff, Kirk Ekena, James E. Hagstrom
  • In vivo inhibition of hepatitis B virus
    Publication number: 20060063731
    Abstract: A process is provided to deliver polynucleotide-based gene expression inhibitors to cells in a mammal in vivo for the purpose of inhibiting gene expression in the cells. Inhibition is sequence-specific and relies on sequence similarity to of the polynucleotide-based gene expression inhibitor and the target nucleic acid molecule. Delivery of the polynucleotide-based gene expression inhibitor can enhance the efficacy of co-delivered small molecule drugs.
    Type: Application
    Filed: September 26, 2005
    Publication date: March 23, 2006
    Inventors: David Lewis, Jon Wolff, Hans Herweijer, James Hagstrom, Aaron Loomis
  • Formation of polyampholytes in the presence of a polyion
    Publication number: 20060063257
    Abstract: Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
    Type: Application
    Filed: October 21, 2005
    Publication date: March 23, 2006
    Inventors: Darren Wakefield, David Rozema, Jon Wolff, Vladimir Trubetskoy, James Hagstrom, Vladimir Budker, Jason Klein, So Wong
  • Intravascular delivery of nucleic acid
    Patent number: 7015040
    Abstract: Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
    Type: Grant
    Filed: December 10, 2004
    Date of Patent: March 21, 2006
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, Vladimir G. Budker, James E. Hagstrom, Julia Hegge, Hans Herweijer
  • Process of making a compound by forming a polymer from a template drug
    Publication number: 20060024828
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Application
    Filed: September 26, 2005
    Publication date: February 2, 2006
    Inventors: Vladimir Trubetskoy, Jon Wolff, Paul Slattum, Lisa Hanson, Vladimir Budker, James Hagstrom
  • Gene expression with covalently modified polynucleotides
    Publication number: 20050272154
    Abstract: A process and compound wherein nucleic acids can be modified with a host of molecules and maintain their ability to be expressed. A modifying chemical attachment of polyions to polynucleotides can be used to facilitate the change of tertiary structure of the nucleic acid and in some cases condensation of nucleic acids into smaller, charged particles useful in delivering the nucleic acid to a cell.
    Type: Application
    Filed: July 26, 2005
    Publication date: December 8, 2005
    Inventors: Jon Wolff, Vladimir Budker, James Hagstrom, Paul Slattum
  • Polymerized formamides for use in delivery of compounds to cells
    Publication number: 20050265957
    Abstract: The invention provides for polycations for condensation and delivery of polynucleotides to cells. Processes for forming the polycations by the polymerization of formamide monomers is also described.
    Type: Application
    Filed: April 8, 2005
    Publication date: December 1, 2005
    Inventors: Sean Monahan, Lisa Nader, Vladimir Budker, James Hagstrom, Paul Slattum, Jon Wolff
  • Inhibition of gene expression by delivery of small interfering RNA to post-embryonic animal cells in vivo
    Publication number: 20050260270
    Abstract: A process is provided to deliver small interfering RNA to cells in vivo for the purpose of inhibiting gene expression in that cell. The small interfering RNA is less than 50 base-pairs in length. This process is practiced on post-embryonic animals. Inhibition is sequence-specific and relies on sequence identity of the small interfering RNA and the target nucleic acid molecule.
    Type: Application
    Filed: April 25, 2005
    Publication date: November 24, 2005
    Inventors: David Lewis, Hans Herweijer, Jon Wolff, James Hagstrom, Aaron Loomis
  • Reversible modification of membrane interaction
    Publication number: 20050250683
    Abstract: An process for the reversible modification of membrane interaction of a compound is described. Modification of membrane interaction can be used to facilitate delivery of molecules to cells in vitro and in vivo. The described modifiers, which are used to reversibly inactivate the membrane active compounds, can also be utilized as cross-linkers or to reverse the charge of a molecule.
    Type: Application
    Filed: May 23, 2003
    Publication date: November 10, 2005
    Inventors: David Rozema, Darren Wakefield, Jon Wolff, Kirk Ekena, James Hagstrom
  • Process of making a compound by forming a polymer from a template drug
    Patent number: 6960690
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Grant
    Filed: December 5, 2001
    Date of Patent: November 1, 2005
    Assignee: Mirns Bio Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson
  • Process for delivering nucleic acids to cardiac tissue
    Publication number: 20050222075
    Abstract: A process for delivering a nucleic acid to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a nucleic acid to a blood vessel, which subsequently delivers the nucleic acid to the cardiac tissue cell. The nucleic acid can be DNA or RNA or plasmid DNA or viral. This process is for purposes of gene therapy, and research.
    Type: Application
    Filed: May 25, 2005
    Publication date: October 6, 2005
    Inventors: Hans Herweijer, Jon Wolff, Matthew Wolff, Larry Whitesell
  • DNA cassette for cellular expression of small RNA
    Publication number: 20050196862
    Abstract: We describe expression cassettes and processes for preparing expression cassettes that can be delivered to animal cells in vivo or in vitro. Delivery of the cassettes results in expression of small RNA transcripts such as siRNA in the cell. The cassettes can by used to inhibit gene expression in the cell and to screen siRNA and other RNA sequences for functional efficacy.
    Type: Application
    Filed: August 29, 2003
    Publication date: September 8, 2005
    Inventors: Christine Wooddell, Hans Herweijer, Jon Wolff
  • Therapeutic fusion protein transgenes
    Publication number: 20050192242
    Abstract: Chimeric antitumor compounds for the treatment of cancer are disclosed. The chimeric compounds may be encoded by a nucleic acid. Delivery of the nucleic acid to cells in vivo provides for in vivo production of the antitumor compounds.
    Type: Application
    Filed: March 31, 2005
    Publication date: September 1, 2005
    Inventors: Zane Neal, Hans Herweijer, Jon Wolff
  • Compound containing a labile disulfide bond
    Patent number: 6936729
    Abstract: A labile disulfide-containing compound under physiological conditions, comprising: the disulfide-containing compound having a labile disulfide bond that is either a disulfide bond that is cleaved more rapidly than oxidized glutathione or a disulfide bond constructed from thiols in which one of the constituent thiols has a lower pKa than glutathione or a disulfide bond that is activated by intramolecular attack from a free thiol.
    Type: Grant
    Filed: February 28, 2001
    Date of Patent: August 30, 2005
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, Sean D. Monahan, Vladimir G. Budker, Paul M. Slattum, David B. Rozema