Patents by Inventor Jon A. Wolff

Jon A. Wolff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Process for generating multilayered particles
    Publication number: 20040067587
    Abstract: We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
    Type: Application
    Filed: October 4, 2002
    Publication date: April 8, 2004
    Inventors: Vladimir S. Trubetskoy, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, So Chun Wong, Jason Klein
  • Compositions and methods for drug delivery using pH sensitive molecules
    Publication number: 20040058446
    Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
    Type: Application
    Filed: July 15, 2003
    Publication date: March 25, 2004
    Inventors: Jon A. Wolff, James E. Hagstrom, David B. Rozema, Sean D. Monahan, Vladimir G. Budker
  • Process of making a compound by forming a polymer from a template drug
    Patent number: 6706922
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Grant
    Filed: November 16, 2001
    Date of Patent: March 16, 2004
    Assignee: Mirus Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy, Paul M. Slattum, Lisa J. Hanson
  • Expression of exogenous polynucleotide sequences in a vertebrate
    Patent number: 6706694
    Abstract: The present invention provides a method for delivering a pharmaceutical polypeptide to the interior of a cardiac cell of a vertebrate in vivo, comprising the step of introducing a preparation comprising a pharmaceutically acceptable injectable carrier and naked polynucleotide operatively coding for the polypeptide into the interstitial space of the heart, whereby the naked polynucleotide is taken up into the interior of the cell and has a pharmacological effect on the vertebrate. In a preferred embodiment wherein the polynucleotide encodes polypeptide immunologically foreign to the vertebrate, the delivery method preferably comprises delivering an immunosuppressive agent to the vertebrate to limit immune responses directed to the polypeptide.
    Type: Grant
    Filed: June 6, 2000
    Date of Patent: March 16, 2004
    Assignees: Vical Incorporated, Wisconsin Alumni Research Foundation
    Inventors: Jon A. Wolff, David J. Duke, Philip L. Felgner
  • Compounds for targeting hepatocytes in vivo
    Publication number: 20040029826
    Abstract: We describe compounds that bind to and are internalized by hepatocytes. Association of these compounds to other molecules or complexes can be used to target the molecules or complexes to hepatocytes in vivo or in vitro.
    Type: Application
    Filed: August 4, 2003
    Publication date: February 12, 2004
    Inventors: Alexander V. Sokoloff, So Wong, Jon A. Wolff, Sean D. Monahan, James Ludtke, Lori Higgs, Darren Wakefield, Magdolna G. Sebestyen
  • Delivery of molecules and complexes to mammalian cells in vivo
    Publication number: 20040023850
    Abstract: Disclosed is a system for providing in vivo delivery of molecules or complexes to extravascular mammalian cells using an intravascular administration route. The molecules or complexes are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate transiently increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the molecule to the cell.
    Type: Application
    Filed: July 28, 2003
    Publication date: February 5, 2004
    Inventors: Jon A. Wolff, Vladimir G. Budker, Hans Herweijer, James E. Hagstrom, Sean D. Monahan, Julia Hegge, Vladimir Subbotin
  • Micellar systems
    Publication number: 20040023393
    Abstract: A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle.
    Type: Application
    Filed: July 25, 2003
    Publication date: February 5, 2004
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20040019007
    Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
    Type: Application
    Filed: June 20, 2003
    Publication date: January 29, 2004
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Compositions and processes using siRNA, amphipathic compounds and polycations
    Publication number: 20040019008
    Abstract: Described is a composition with low toxicity comprising an amphipathic compound and a polycation, useful for delivering siRNA to a cell. The composition may be used in the process of delivering a siRNA to an animal cell, or more particularly a mammal cell, in a multi-well format.
    Type: Application
    Filed: July 17, 2003
    Publication date: January 29, 2004
    Inventors: David L. Lewis, James E. Hagstrom, Hans Herweijer, Aaron G. Loomis, Sean D. Monahan, Jon A. Wolff, Vladimir Trubetskoy
  • Micellar systems
    Patent number: 6673612
    Abstract: A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery.
    Type: Grant
    Filed: February 21, 2002
    Date of Patent: January 6, 2004
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker
  • Novel methods for the delivery of polynucleotides to cells
    Publication number: 20030235916
    Abstract: Process are described for the delivery of a polynucleotide to a cell. The process comprises forming a salt stable complex between the polynucleotide and a cationic surfactant. Ternary complexes are also made by associating an amphipathic compound with the binary complex. The resultant complexes are suitable for delivery of the polynucleotide to cells in vitro and in vivo.
    Type: Application
    Filed: June 16, 2003
    Publication date: December 25, 2003
    Inventors: Sean D. Monahan, Lisa Nader, Jon A. Wolff, Vladimir G. Budker, James E. Hagstrom
  • Charge reversal of polyion complexes and treatment of peripheral occlusive disease
    Publication number: 20030236214
    Abstract: A process is described for the delivery of a therapeutic polynucleotide to a tissue suffering from or potentially suffering from ischemia. An ionic polymer is utilized in “recharging” (another layer having a different charge) a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The resulting recharged complex can be formed with an appropriate amount of positive or negative charge such that the resulting complex has the desired net charge.
    Type: Application
    Filed: April 15, 2003
    Publication date: December 25, 2003
    Inventors: Jon A. Wolff, Vladimir G. Budker, Julia Hegge, James E. Hagstrom, Vladimir Trubetskoy
  • Processes for inhibiting gene expression using polynucleotides
    Publication number: 20030228691
    Abstract: A process is provided for inhibition of specific gene expression in an animal cell by delivering a combination of RNA function inhibitors. Using a combination of inhibitors results in improved efficacy of gene expression inhibition. The process can be used to reduce gene expression in cells in vitro and in vivo.
    Type: Application
    Filed: May 19, 2003
    Publication date: December 11, 2003
    Inventors: David L. Lewis, David B. Rozema, Jon A. Wolff, James E. Hagstrom, Hans Herweijer
  • Compositions and processes for inhibiting gene expression using polynucleotides
    Publication number: 20030224055
    Abstract: Compositions are provided for delivery of polynucleotides to cells for the purpose of inhibiting gene expression. Antisense polynucleotide-containing complexes are described. The salt and serum stability and small size of the complexes permits delivery to cells in vitro and in vivo.
    Type: Application
    Filed: May 28, 2003
    Publication date: December 4, 2003
    Inventors: David L. Lewis, David B. Rozema, Darren Wakefield, Hans Herweijer, Jon A. Wolff, James E. Hagstrom
  • Reversible modification of membrane interaction
    Publication number: 20030220264
    Abstract: An process for the reversible modification of membrane interaction of a compound is described. Modification of membrane interaction can be used to facilitate delivery of molecules to cells in vitro and in vivo. The described modifiers, which are used to reversibly inactivate the membrane active compounds, can also be utilized as cross-linkers or to reverse the charge of a molecule.
    Type: Application
    Filed: May 23, 2003
    Publication date: November 27, 2003
    Inventors: David B. Rozema, Darren Wakefield, Jon A. Wolff, Kirk Ekena, James E. Hagstrom
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20030216347
    Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
    Type: Application
    Filed: June 20, 2003
    Publication date: November 20, 2003
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Compositions and methods for drug delivery using pH sensitive molecules
    Publication number: 20030199090
    Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
    Type: Application
    Filed: February 26, 2002
    Publication date: October 23, 2003
    Inventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Compositions and methods for drug delivery using pH sensitive molecules
    Patent number: 6630351
    Abstract: A system relating to the delivery of desired compounds (e.g., drugs and nucleic acids) into cells using pH-sensitive delivery systems. The system provides compositions and methods for the delivery and release of a compound to a cell.
    Type: Grant
    Filed: June 7, 2000
    Date of Patent: October 7, 2003
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Expression of exogenous polynucleotide sequences in a vertebrate
    Publication number: 20030186913
    Abstract: The present invention provides a method for delivering a pharmaceutical polypeptide to the interior of a cardiac cell of a vertebrate in vivo, comprising the step of introducing a preparation comprising a pharmaceutically acceptable injectable carrier and naked polynucleotide operatively coding for the polypeptide into the interstitial space of the heart, whereby the naked polynucleotide is taken up into the interior of the cell and has a pharmacological effect on the vertebrate. In a preferred embodiment wherein the polynucleotide encodes polypeptide immunologically foreign to the vertebrate, the delivery method preferably comprises delivering an immunosuppressive agent to the vertebrate to limit immune responses directed to the polypeptide.
    Type: Application
    Filed: February 10, 2003
    Publication date: October 2, 2003
    Applicants: Vical Incorporated, Wisconsin Alumni Research Foundation
    Inventors: Jon A. Wolff, David J. Duke, Philip L. Felgner
  • Intravascular delivery of non-viral nucleic acid
    Patent number: 6627616
    Abstract: Disclosed is a process for transfecting genetic material into a mammalian cell to alter endogenous properties of the cell. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as a tail vein or artery. Prior to insertion, subsequent to insertion, or concurrent with insertion the permeability of the vessel is increased thereby the genetic material is delivered to the parenchymal cell altering endogenous properties of the cell.
    Type: Grant
    Filed: November 23, 1999
    Date of Patent: September 30, 2003
    Assignee: Mirus Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema