Patents by Inventor Jon Wolff

Jon Wolff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Intravascular delivery of nucleic acid
    Publication number: 20050182013
    Abstract: Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
    Type: Application
    Filed: December 10, 2004
    Publication date: August 18, 2005
    Inventors: Jon Wolff, Vladimir Budker, James Hagstrom, Julia Hegge
  • Formation of polyampholytes in the presence of a polyion
    Publication number: 20050170505
    Abstract: Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
    Type: Application
    Filed: January 28, 2005
    Publication date: August 4, 2005
    Inventors: Darren Wakefield, David Rozema, Jon Wolff, Vladimir Trubetskoy, James Hagstrom, Vladimir Budker, Jason Klein, So Wong
  • Compositions and methods for drug delivery using pH sensitive molecules
    Patent number: 6919091
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: March 11, 2002
    Date of Patent: July 19, 2005
    Assignee: Mirus Corporation
    Inventors: Vladimir S. Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jon A. Wolff, David B. Rozema, Sean D. Monahan
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20050153451
    Abstract: Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
    Type: Application
    Filed: October 28, 2004
    Publication date: July 14, 2005
    Inventors: Jon Wolff, Vladimir Budker, James Hagstrom, Hans Herweijer, Julia Hegge, Paul Slattum, Sean Monahan, David Rozema
  • Compositions and processes using siRNA, amphipathic compounds and polycations
    Publication number: 20050143332
    Abstract: Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.
    Type: Application
    Filed: May 14, 2004
    Publication date: June 30, 2005
    Inventors: Sean Monahan, David Lewis, Hans Herweijer, Jon Wolff, James Hagstrom, Aaron Loomis, Vladimir Trubetskoy, Lori Higgs
  • Process of delivering a virally encapsulated polynucleotide or viral vector to a parenchymal cell via the vascular system
    Publication number: 20050129660
    Abstract: A process for delivering a polynucleotide into a parenchymal cell in a mammal, comprising, transporting the polynucleotide into a vessel communicating with the parenchymal cell in tissue or organ of the mammal such that the polynucleotide is transfected into the parenchymal cell. More specifically, the polynucleotide consists of a viral vector.
    Type: Application
    Filed: December 11, 2003
    Publication date: June 16, 2005
    Inventors: James Hagstrom, Jon Wolff, Vladimir Budker, Julia Hegge, Hans Herweijer
  • Compositions and methods for drug delivery using pH sensitive molecules
    Publication number: 20050123600
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Application
    Filed: January 28, 2005
    Publication date: June 9, 2005
    Inventors: Vladimir Trubetskoy, James Hagstrom, Vladimir Budker, Jon Wolff, David Rozema, Sean Monahan
  • Polynucleotide complex delivery
    Patent number: 6897068
    Abstract: Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
    Type: Grant
    Filed: February 27, 2002
    Date of Patent: May 24, 2005
    Assignee: Mirus Bio Corporation
    Inventors: Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, DAvid B. Rozema, Paul M. Slattum
  • Formation of polyampholytes in the presence of a polyion
    Patent number: 6881576
    Abstract: A polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: March 11, 2002
    Date of Patent: April 19, 2005
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy
  • In vitro process for selecting phage resistant to blood inactivation
    Patent number: 6872519
    Abstract: The present invention relates to compositions and methods for the selection and use of surface exposed epitopes. The present invention includes in vivo and in vitro phage peptide diplay methods for the identification and selection of peptides and peptide associated factors with desired properties (e.g., targeting specificity, stability, etc.). The present invention further provides methods and compositions for the isolation and identification of peptide-specific antibodies. The present invention also includes methods and compositions employing nuclear localization signals for enhanced nuclear transport and expression of DNA.
    Type: Grant
    Filed: April 27, 2000
    Date of Patent: March 29, 2005
    Assignee: Mirus Bio Corporation
    Inventors: Alexander V. Sokoloff, Jon A. Wolff
  • Process for delivering nucleic acids to cardiac tissue
    Patent number: 6867196
    Abstract: A process for delivering a nucleic acid to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a nucleic acid to a blood vessel, which subsequently delivers the nucleic acid to the cardiac tissue cell. The nucleic acid can be DNA or RNA or plasmid DNA or viral. This process is for purposes of gene therapy, and research.
    Type: Grant
    Filed: June 11, 1999
    Date of Patent: March 15, 2005
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, Hans Herweijer, Larry F. Whitesell, Matthew R. Wolff, Sean D. Monahan, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Inhibition of gene function by delivery of polynucleotide-based gene expression inhibitors to mammalian cells in vivo
    Publication number: 20050037989
    Abstract: A process is provided to deliver polynucleotide-based gene expression inhibitors to cells in a mammal in vivo for the purpose of inhibiting gene expression in the cells. Inhibition is sequence-specific and relies on sequence similaroty of the polynucleotide-based gene expression inhibitor and the target nucleic acid molecule. Delivery of the polynucleotide-based gene expression inhibitor can enhance the efficacy of co-delivered small molecule drugs.
    Type: Application
    Filed: June 23, 2004
    Publication date: February 17, 2005
    Inventors: David Lewis, Jon Wolff, Hans Herweijer, James Hagstrom, Aaron Loomis, Andrew Spencer
  • Polyampholytes for delivering polyions to a cell
    Publication number: 20050037496
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Application
    Filed: July 26, 2004
    Publication date: February 17, 2005
    Inventors: David Rozema, Vladimir Budker, James Hagstrom, Vladimir Trubetskoy, Jon Wolff, Sean Monahan, Paul Slattum
  • Compound containing a labile disulfide bond
    Publication number: 20050020518
    Abstract: A labile disulfide-containing compound under physiological conditions containing a labile disulfide bond and a transduction signal.
    Type: Application
    Filed: February 8, 2001
    Publication date: January 27, 2005
    Inventors: Jon Wolff, Sean Monahan, Vladimir Budker, Paul Slattum, David Rozema
  • Process for utilizing epitopes recognized by natural antibodies
    Publication number: 20040259170
    Abstract: The present invention relates to compositions and methods for the selection and use of surface exposed epitopes. The present invention includes in vivo and in vitro phage peptide diplay methods for the identification and selection of peptides and peptide associated factors with desired properties (e.g., targeting specificity, stability, etc.). The present invention further provides methods and compositions for the isolation and identification of peptide-specific antibodies. The present invention also includes methods and compositions employing nuclear localization signals for enhanced nuclear transport and expression of DNA.
    Type: Application
    Filed: March 8, 2004
    Publication date: December 23, 2004
    Inventors: Alexander V. Sokoloff, Jon A. Wolff
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20040259828
    Abstract: Disclosed is a system for providing nucleic acid expression in a cell. A polynucleotide is inserted into a mammalian vasculature. The vessel permeability is increased and the polynucleotide is delivered to the cell where it enhances the endogenous properties of the cell.
    Type: Application
    Filed: May 4, 2004
    Publication date: December 23, 2004
    Inventors: Jon A. Wolff, Vladimir G. Budker, James E. Hagstrom, Sean D. Monahan, David B. Rozema, Paul M. Slattum
  • Inhibition of gene expression by delivery of small interfering RNA to post-embryonic animal cells in vivo
    Publication number: 20040229358
    Abstract: A process is provided to deliver small interfering RNA to cells in vivo for the purpose of inhibiting gene expression in that cell. The small interfering RNA is less than 50 base-pairs in length. This process is practiced on post-embryonic animals. Inhibition is sequence-specific and relies on sequence identity of the small interfering RNA and the target nucleic acid molecule.
    Type: Application
    Filed: November 7, 2001
    Publication date: November 18, 2004
    Inventors: David L. Lewis, Jon A. Wolff, Aaron G. Loomis, James E, Hagstrom, Hans Herweijer
  • Chelating systems for use in the delivery of compounds to cells
    Patent number: 6818626
    Abstract: Chelator containing compounds are utilized in the delivery of molecules, polymers, nucleic acids and genes to animal cells. At least one chelator such as crown ether is attached to a polymer and then associated with another polymer such as DNA. An ion is then added to the mixture thereby forming condensed DNA. In condensed form and in complex with the chelator, DNA can be delivered to a cell.
    Type: Grant
    Filed: January 21, 1999
    Date of Patent: November 16, 2004
    Assignee: Mirus Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Sean D. Monahan, Vladimir Budker, Vladimir Trubetskoy, Paul M. Slattum
  • Devices and processes for distribution of genetic material to mammalian limb
    Publication number: 20040224879
    Abstract: A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from ischemia. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.
    Type: Application
    Filed: May 5, 2003
    Publication date: November 11, 2004
    Inventors: Jon A. Wolff, Vladimir G. Budker, James E. Hagstrom, Julia Hegge, Vladimir Subbotin
  • Method for single-step attachment of a label to target molecules
    Publication number: 20040210044
    Abstract: Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Application
    Filed: April 15, 2003
    Publication date: October 21, 2004
    Inventors: Paul M. Slattum, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker