Patents by Inventor Jon Wolff

Jon Wolff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Process for generating multilayered particles
    Publication number: 20040203154
    Abstract: We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
    Type: Application
    Filed: March 8, 2004
    Publication date: October 14, 2004
    Inventors: Vladimir Trubetskoy, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, So Chun Wong, Jason Klein
  • Endosomolytic polymers
    Publication number: 20040198687
    Abstract: We describe pH-sensitive endosomolytic polymers, delivery particles containing pH-sensitive endosomolytic polymers. The described particles are capable of delivering polynucleotides to cells from the peripheral circulation with subsequent release from endosomes. The endosomolytic polymers are inactive outside the cell but disrupt membranes upon exposure to an acidified endosomal compartment.
    Type: Application
    Filed: April 1, 2004
    Publication date: October 7, 2004
    Inventors: David B. Rozema, Darren Wakefield, Jon A. Wolff, Vladimir Trubetskoy, Vladimir G. Budker, James E. Hagstrom, Aaron G. Loomis, Sean D. Monahan, Paul M. Slattum
  • Chelating systems for use in the delivery of compounds to cells
    Publication number: 20040197318
    Abstract: Chelator containing compounds are utilized in the delivery of molecules, polymers, nucleic acids and genes to animal cells. At least one chelator such as crown ether is attached to a polymer and then associated with another polymer such as DNA. An ion is then added to the mixture thereby forming condensed DNA. In condensed form and in complex with the chelator, DNA can be delivered to a cell.
    Type: Application
    Filed: April 22, 2004
    Publication date: October 7, 2004
    Inventors: Jon A. Wolff, Sean D. Monahan, James E. Hagstrom, Paul M. Slattum, Vladimir G. Budker, Vladimir Trubetskoy
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20040198678
    Abstract: Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
    Type: Application
    Filed: June 7, 2001
    Publication date: October 7, 2004
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Polyampholytes for delivering polyions to a cell
    Patent number: 6794189
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: May 10, 2002
    Date of Patent: September 21, 2004
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy
  • Covalent modification of RNA for in vitro and in vivo delivery
    Publication number: 20040167090
    Abstract: The post-synthetic modification of RNA for the delivery of the RNA to a mammalian cell is described. The modifications enhance resistant of the RNA to nuclease digestion and delivery of the RNA to the cell whether the RNA is delivered alone or in combination with a transfection agent. Activity of the RNA in the cell is maintained.
    Type: Application
    Filed: February 19, 2004
    Publication date: August 26, 2004
    Inventors: Sean D. Monahan, Vladimir G. Budker, Lisa Nader, Vladimir Subbotin, Jon A. Wolff
  • Process of making a compound by forming a polymer from a template drug
    Publication number: 20040161463
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Application
    Filed: January 12, 2004
    Publication date: August 19, 2004
    Inventors: Vladimir Trubetskoy, Jon A. Wolff, Paul M. Slattum, Lisa Hanson, Vladimir G. Budker, James E. Hagstrom
  • Delivery of siRNA to cells using polyampholytes
    Publication number: 20040162235
    Abstract: A polyampholyte is utilized in a complex with siRNA for purposes of siRNA delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered a cell in vivo or in vitro.
    Type: Application
    Filed: February 18, 2003
    Publication date: August 19, 2004
    Inventors: Vladimir S. Trubetskoy, David B. Rozema, Sean D. Monahan, Vladimir G. Budker, James E. Hagstrom, Jon A. Wolff
  • Process for delivering sirna to cardiac muscle tissue
    Publication number: 20040157790
    Abstract: A process for delivering a polynucleotide to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a polynucleotide into a blood vessel and increasing permeability of the blood vessel to the polynucleotide. The polynucleotide can be a small interfering RNA or microRNA and inhibit gene expression in the cell.
    Type: Application
    Filed: February 3, 2004
    Publication date: August 12, 2004
    Inventors: Hans Herweijer, Julia Hegge, Jon A. Wolff, David L. Lewis
  • Compounds and processes for single-pot attachment of a label to nucleic acid
    Publication number: 20040152084
    Abstract: Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Application
    Filed: January 31, 2003
    Publication date: August 5, 2004
    Inventors: Paul M. Slattum, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker
  • Compounds and processes for single-pot attachment of a label to siRNA
    Publication number: 20040146867
    Abstract: Compounds and methods are provided for a single-pot covalent attachment of a label to an siRNA comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Application
    Filed: January 24, 2003
    Publication date: July 29, 2004
    Inventors: Paul M. Slattum, Vladimir G. Budker, James E. Hagstrom, Jon A. Wolff
  • Compositions and methods for drug delivery using amphiphile binding molecules
    Publication number: 20040137625
    Abstract: The present invention relates to the delivery of desired compounds (e.g., nucleic acids) into cells using noncovalent delivery systems which include complexing nucleic acids, amphipathic binding agents, and amphiphiles.
    Type: Application
    Filed: January 12, 2004
    Publication date: July 15, 2004
    Inventors: Jon A. Wolff, James E. Hagstrom, Sean D. Monahan, Vladimir Budker, David B. Rozema, Paul M. Slattum
  • Devices and processes for distribution of genetic material to mammalian limb
    Publication number: 20040136960
    Abstract: Disclosed is a process for functionally distributing genetic material to mammalian limb muscle cells comprising inserting genetic material into a limb vessel. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the genetic material with the use of an externally applied cuff to increase vascular permeability.
    Type: Application
    Filed: January 10, 2003
    Publication date: July 15, 2004
    Inventors: Jon A. Wolff, Vladimir Budker
  • Compositions and processes using siRNA, amphipathic compounds and polycations
    Publication number: 20040137064
    Abstract: Described is a composition with low toxicity comprising an amphipathic compound and a polycation, useful for delivering siRNA to a cell. The composition may be used in the process of delivering a siRNA to an animal cell, or more particularly a mammal cell, in a multi-well format.
    Type: Application
    Filed: January 15, 2003
    Publication date: July 15, 2004
    Inventors: David L. Lewis, Hans Herweijer, Sean D. Monahan, Jon A. Wolff, James E. Hagstrom, Aaron G. Loomis
  • Enhancing intermolecular integration of nucleic acids using integrator complexes
    Publication number: 20040126887
    Abstract: We disclose compositions and processes for enhancing transposon mediated integration of a nucleic acid molecule into another target nucleic acid molecule. Integration by an integrator complex is enhanced by cationic reagents.
    Type: Application
    Filed: November 8, 2002
    Publication date: July 1, 2004
    Inventors: Christine Wooddell, Hans Herweijer, Jon A. Wolff
  • Nucleic acid injected into hapatic vein lumen and delivered to primate liver
    Publication number: 20040110697
    Abstract: Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
    Type: Application
    Filed: December 5, 2002
    Publication date: June 10, 2004
    Inventors: Jon A. Wolff, Julia Hegge, James E. Hagstrom, Vladimir G. Budker
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20040106567
    Abstract: The process comprises designing a polynucleotide, such as an siRNA, for transfection. The polynucleotide is inserted into a mammalian vessel such as an artery. Prior to insertion, subsequent to insertion, or concurrent with insertion volume in the vessel is increased allowing the polynucleotide delivery to the parenchymal cell.
    Type: Application
    Filed: June 30, 2003
    Publication date: June 3, 2004
    Inventors: James E. Hagstrom, Jon A. Wolff, Sean D. Monahan, David B. Rozema, Vladimir G. Budker, Paul M. Slattum, David L. Lewis
  • Compositions and methods for drug delivery using amphiphile binding molecules
    Patent number: 6740643
    Abstract: The present invention relates to the delivery of desired compounds (e.g., nucleic acids) into cells using noncovalent delivery systems which include complexing nucleic acids, amphipathic binding agents, and amphiphiles.
    Type: Grant
    Filed: November 29, 2000
    Date of Patent: May 25, 2004
    Assignee: Mirus Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Sean D. Monahan, Vladimir Budker, David B. Rozema, Paul M. Slattum
  • Process for generating multilayered particles
    Patent number: 6740336
    Abstract: We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
    Type: Grant
    Filed: October 4, 2002
    Date of Patent: May 25, 2004
    Assignee: Mirus Corporation
    Inventors: Vladimir S. Trubetskoy, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, So Chun Wong, Jason Klein
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20040072785
    Abstract: A process is described for the delivery of a therapeutic polynucleotide to a tissue suffering from or potentially suffering from ischemia. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as an artery or a vein. Prior to insertion, subsequent to insertion, or concurrent with insertion the volume of the tissue is increased such that the genetic material is delivered to the parenchymal cell.
    Type: Application
    Filed: July 28, 2003
    Publication date: April 15, 2004
    Inventors: Jon A. Wolff, Sean D. Monahan, James E. Hagstrom, David B. Rozema, Vladimir G. Budker, Paul M. Slattum