Abstract: A medical connector can include a body having a fluid path with an inlet port, an outlet port, a luer portion, and a post extending through a fluid path. An arm having a latch member is coupled to the body to engage a ridge or thread on an exterior surface of a reciprocal connector. The arm may also include an extension. When the connector is in an open position, the latch member restricts retraction of the reciprocal connector from the outlet port. A seal is coupled to the body and includes a flap extending across the outlet port or a bellows to compress along an axis. In a closed position, the post restricts flow through the luer portion. In an open position, the post permits flow through the luer portion.

Abstract: In certain embodiments, a system, a computer-implemented method, and computer-readable medium are disclosed for enhanced ophthalmic visualization. A plurality of images corresponding to different portions of the electromagnetic spectrum are obtained and combined, such as by pixel-wise subtraction to obtain a combined image. The images may be weighted with weights selected to enhance visualization of features, such as layers of the retina or features corresponding to pathologies. The combined image may be processed, such as by a machine learning model, to extract features.

Abstract: Implementations described herein are directed to enabling collaborative ranking of interpretations of spoken utterances based on data that is available to an automated assistant and third-party agent(s), respectively. The automated assistant can determine first-party interpretation(s) of a spoken utterance provided by a user, and can cause the third-party agent(s) to determine third-party interpretation(s) of the spoken utterance provided by the user. In some implementations, the automated assistant can select a given interpretation, from the first-party interpretation(s) and the third-party interpretation(s), of the spoken utterance, and can cause a given third-party agent to satisfy the spoken utterance based on the given interpretation.

Abstract: Systems and methods for a powered, robotic exoskeleton, or exosuit, for a user's limbs and body are provided. The exosuit may be equipped with airbag devices mounted at various locations on the suit. The exosuit may include on-board computing equipment that can sense, compute control commands in real-time, and actuate limbs and airbags to restore stability (fall prevention) and minimize injuries due to falls, should they happen (fall protection).

Abstract: A method may include generating, by a camera having a view of an interior portion of a computer-numerically-controlled machine, an image comprising a pattern. The image can be transformed into a set of machine instructions for controlling the computer-numerically-controlled machine to effect a change in a material. The change can correspond to at least a portion of the pattern. At least one machine instruction from the set of machine instructions can be executed to control the computer-numerically-controlled machine to effect at least a portion of the change. The execution can include operating, in accordance with the at least one machine instruction, a tool coupled with the computer-numerically-controlled machine. The tool can be configured to effect the change on the material. Related systems and articles of manufacture, including computer program products, are also provided.

Abstract: Systems and methods for gas lift in the main bore and lateral legs of a multilateral well. The systems and methods can allow for gas lift below the junction of a multilateral well.

Abstract: Compositions and methods for cellular genome engineering that permit simple and efficient targeted knock-in of a CAR and simultaneous knockout of individual genes are described. The compositions and methods are especially applicable to massively parallel engineering, selection, and identification of CAR T cell variants exhibiting a desired phenotype. AAV vectors containing crRNA and CAR expression cassettes and homology arms for targeted genomic integration thereof are provided. Also provided are libraries containing a plurality of AAV vectors and methods of use thereof in screens for identifying desirable CAR T cell variants. Methods of treatment using CAR T cell variants exhibiting improvements in one or more phenotypes are also provided.

Abstract: The present invention includes compositions and methods for identification of membrane targets for enhancement of T cell activity against a disease, disorder or condition, and/or enhancing T cell anti-tumor activity in a subject in need thereof. In some embodiments, the disease is cancer. In further embodiments, the cancer is glioblastoma (GBM) or breast cancer.

Abstract: A method may include generating, by a camera having a view of an interior portion of a computer-numerically-controlled machine, an image comprising a pattern. The image can be transformed into a set of machine instructions for controlling the computer-numerically-controlled machine to effect a change in a material. The change can correspond to at least a portion of the pattern. At least one machine instruction from the set of machine instructions can be executed to control the computer-numerically-controlled machine to effect at least a portion of the change. The execution can include operating, in accordance with the at least one machine instruction, a tool coupled with the computer-numerically-controlled machine. The tool can be configured to effect the change on the material. Related systems and articles of manufacture, including computer program products, are also provided.

Abstract: A method for dithering can include receiving, at a computer numerically controlled machine comprising a laser, a motion plan corresponding to a first image. The output of the laser can be dithered, in accordance with the motion plan, to effect a change in a material within an interior space of the computer numerically controlled machine. The change can substantially reproduce at least a portion of the first image on the material. The dithering can include providing laser energy to the material at a native resolution based at least on a spot size of the laser. The spot size can be determined based at least on one or more parameters of the computer numerically controlled machine and/or one or more properties of the material. The laser energy can be delivered at locations separated by a distance no less than the spot size.

Abstract: A method may include generating, by a camera having a view of an interior portion of a computer-numerically-controlled machine, an image comprising a pattern. The image can be transformed into a set of machine instructions for controlling the computer-numerically-controlled machine to effect a change in a material. The change can correspond to at least a portion of the pattern. At least one machine instruction from the set of machine instructions can be executed to control the computer-numerically-controlled machine to effect at least a portion of the change. The execution can include operating, in accordance with the at least one machine instruction, a tool coupled with the computer-numerically-controlled machine. The tool can configured to effect the change on the material. Related systems and articles of manufacture, including computer program products, are also provided.

Abstract: The present invention includes dead-guide RNA (dgRNA) libraries and methods of use in immunology and immunotherapy thereof. Also provided are engineered primary or chimeric antigen receptor (CAR) T cells that overexpress Prodh2, Ccnblip1, Sreklip1, or Wdr37or a fragment thereof, and methods of use in immunology and immunothereapy thereof.

Abstract: A method for dithering can include receiving, at a computer numerically controlled machine comprising a laser, a motion plan corresponding to a first image. The output of the laser can be dithered, in accordance with the motion plan, to effect a change in a material within an interior space of the computer numerically controlled machine. The change can substantially reproduce at least a portion of the first image on the material. The dithering can include providing laser energy to the material at a native resolution based at least on a spot size of the laser. The spot size can be determined based at least on one or more parameters of the computer numerically controlled machine and/or one or more properties of the material. The laser energy can be delivered at locations separated by a distance no less than the spot size.

Abstract: A method for dithering can include receiving, at a computer numerically controlled machine comprising a laser, a motion plan corresponding to a first image. The output of the laser can be dithered, in accordance with the motion plan, to effect a change in a material within an interior space of the computer numerically controlled machine. The change can substantially reproduce at least a portion of the first image on the material. The dithering can include providing laser energy to the material at a native resolution based at least on a spot size of the laser. The spot size can be determined based at least on one or more parameters of the computer numerically controlled machine and/or one or more properties of the material. The laser energy can be delivered at locations separated by a distance no less than the spot size.

Abstract: A packoff device is disclosed for sealing an annulus within a wellbore, and for bypassing the sealed annulus. The packoff device may include a mandrel having a bore formed axially therethrough and first and second ports extending radially from the bore. A sealing element that extends radially-outwardly from the mandrel may be positioned axially between the first and second ports, and may create a seal within an annulus between the mandrel and a casing, to isolate a portion of the annulus above the sealing element from a portion below the sealing element. A sleeve within the bore may, with the mandrel, form a channel providing fluid communication between the first and second ports. The sleeve may be movable between open and closed states. The first and second ports may be unobstructed by the sleeve in the open state, one or more may be obstructed in the closed state.

Abstract: A base cell of a gate array architecture includes an increased number of transistors that can be interconnected or not interconnected so as to realize similar advantages as a having a library of transistors of different sizes. In one embodiment, each column a base cell contains two PMOS transistors and two NMOS transistors connected so as to share a single polysilicon (“poly”) gate electrode. Such an arrangement of the transistors in metal only programmable base cell architecture provides three different P and N transistor widths per poly gate and may provide nine different combinations transistor widths for P and N for design. The number of gate electrodes is minimized and their arrangement simplified such that the size of the base cell may be same size compared to traditional gate array base cell with four transistors. Moreover, only a single type of base cell need be provided, simplifying layout and design.

Abstract: A packoff device is disclosed for sealing an annulus within a wellbore, and for bypassing the sealed annulus. The packoff device may include a mandrel having a bore formed axially therethrough and first and second ports extending radially from the bore. A sealing element that extends radially-outwardly from the mandrel may be positioned axially between the first and second ports, and may create a seal within an annulus between the mandrel and a casing, to isolate a portion of the annulus above the sealing element from a portion below the sealing element. A sleeve within the bore may, with the mandrel, form a channel providing fluid communication between the first and second ports. The sleeve may be movable between open and closed states. The first and second ports may be unobstructed by the sleeve in the open state, one or more may be obstructed in the closed state.

Abstract: A packoff device is disclosed for sealing an annulus within a wellbore, and for bypassing the sealed annulus. The packoff device may include a mandrel having a bore formed axially therethrough and first and second ports extending radially from the bore. A sealing element that extends radially-outwardly from the mandrel may be positioned axially between the first and second ports, and may create a seal within an annulus between the mandrel and a casing, to isolate a portion of the annulus above the sealing element from a portion below the sealing element. A sleeve within the bore may, with the mandrel, form a channel providing fluid communication between the first and second ports. The sleeve may be movable between open and closed states. The first and second ports may be unobstructed by the sleeve in the open state, one or more may be obstructed in the closed state.

Abstract: A packoff device is disclosed for sealing an annulus within a wellbore, and for bypassing the sealed annulus. The packoff device may include a mandrel having a bore formed axially therethrough and first and second ports extending radially from the bore. A sealing element that extends radially-outwardly from the mandrel may be positioned axially between the first and second ports, and may create a seal within an annulus between the mandrel and a casing, to isolate a portion of the annulus above the sealing element from a portion below the sealing element. A sleeve within the bore may, with the mandrel, form a channel providing fluid communication between the first and second ports. The sleeve may be movable between open and closed states. The first and second ports may be unobstructed by the sleeve in the open state, one or more may be obstructed in the closed state.

Abstract: The present invention relates generally to methods for generating single stranded nucleic acid molecules following enhanced solid phase polynucleotide amplification. The present invention employs an amplification reaction using primers with differential priming properties at particular annealing conditions or an immobilized primer nested between two aqueous phase primers. Thus, by primer design, solid support primer participation is enhanced relative to aqueous phase primers. The subject invention further provides methods for labeling solid matrices with single and double stranded nucleic acid molecules. Kits for generating single stranded nucleic acid molecules and for conducting amplification reactions also form part of the present invention. The present invention further provides amplification systems for the generation of single stranded nucleic acid molecules optionally labelled with a reporter molecule and their use inter alia as labels, primers and probes.