Abstract: The invention provides a method and a kit for combined virus inactivation and capture/purification of a feed containing a protein of interest. The method comprises the steps of: contacting a feed comprising a protein of interest with negatively charged particles or media, conditioning the particles or media such that the pH is acidic thereby inactivating said virus, and eluting said protein of interest.

Abstract: The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.

Abstract: The current disclosure describes a method for purifying a protein, wherein said protein is present in a feed, and wherein said method comprises a multimodal chromatography step, wherein said feed is contacted with a multimodal ion exchanger comprising a ligand with a hydrophobic moiety and a charged moiety and wherein binding of said protein of interest to said exchanger occurs under high conductivity conditions.

Abstract: A sampler for use with a bioreactor for growing a cell culture is disclosed. In one embodiment, the bioreactor includes a structured fixed bed including a removable sample portion for recovering a sample of cells from the cell culture. Related apparatuses and methods are also disclosed.

Abstract: The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.

Abstract: The current disclosure concerns a system for producing biomolecules comprising a bioreactor including a chamber (1) suitable for receiving a liquid comprising cells and viral particles; and a concentrator (2), wherein said concentrator is equipped with a retentate conduit (300, 303) suitable for collecting said retentate and facilitating recirculating of the retentate to an input of said bioreactor or to an input of an intermediate vessel (4) positioned between said concentrator and said bioreactor. In a second and third aspect the disclosure concerns a method for producing biomolecules and the use of the disclosed system for the production of biomolecules.

Abstract: A disposable material processing apparatus, useable as a bioreactor or fermenter, includes a hollow tank (101) and a mixing paddle (110) disposed within the interior of the tank and adapted to mix contents therein. The paddle may be isolated within a flexible sleeve (140). Various functional elements, such as a sparger, sensor, material extraction conduit, material addition conduit, and/or heat exchange element may be provided, and optionally arranged to travel with the paddle within the tank interior. Baffles may protrude into a mixing tank to enhance mixing. A tank and/or sleeve may comprise polymeric film materials.

Abstract: A method and a kit for cell culture clarification, as well as a system or apparatus for clarifying a cell culture and purifying biomolecules of interest, are described. The cell culture comprises at least one biomolecule of interest. The method comprises adding one or more compounds to the cell culture that allow the formation of floccules of solutes and/or particulates in said cell culture harvest, adding an amount of diatomaceous earth (DE) to said cell culture harvest, agitating the obtained solution, and transferring said solution to a filtration vessel comprising a support filter having a surface, whereby the diatomaceous earth in said solution forms a layer on said surface and filtering said harvest solution through said layer and support filter.

Abstract: Disclosed systems and methods relate to determining an intensity level of an exercise using a photoplethysmogram (PPG) sensor. A method of determining an intensity level of an exercise for a user according to one embodiment of the present disclosure includes detecting, by a device, body signals from the user using a PPG sensor. The method includes determining, by the device, a heart rate of the user based on the body signals. The method includes determining, by the device, an error in the heart rate. The method also includes determining, by the device, the intensity level of the exercise for the user based at least on the error.

Abstract: An automated method for the production of cells and/or biomolecules such as protein or peptides includes culturing cells in at least one high cell density bioreactor, thereby fluidly connecting said bioreactor with a culture medium supply and a gas or gaseous mixture; fluidly connecting said bioreactor with a downstream unit; and growing cells to a density at least 50 million cells per ml. The total volume of the bioreactor is at least 10 liters. A system suitable for implementation of the automated method above is a small-scale and cupboard-sized system, which can be placed in a portable clean room.

Abstract: A lock ring spreader is capable of being mounted to a lock ring having a first end and a second end. The lock ring spreader comprises a first mount that is mountable to the lock ring near the first end of the lock ring and a second mount that is mountable to the lock ring near the second end of the lock ring. A crank arm is pivotally coupled to the first mount and extends radially outward from the first mount relative to the lock ring. A link arm is pivotally coupled to the second mount and the crank arm, and extends radially outward from the second mount relative to the lock ring. Pivoting the crank arm radially inward relative to the lock ring pivots the link arm relative to the crank arm and expands the distance between the first mount and the second mount.

Abstract: The invention provides a method and a system for the production of viruses or virus derived products. The system comprises a containment enclosure provided with a single entry means and a single exit means which are movable between a closed position and an open position. The containment enclosure comprises at least one production unit comprising at least one bioreactor for culturing the virus, said bioreactor is provided with at least one inlet and at least one outlet; at least one purification unit fluidly connected to the production unit and comprising at least one purification means having at least one inlet and at least one outlet. The system further comprises at least one inactivation and/or at least one gas decontamination unit. The containment enclosure comprises at least one process control device connected to the exit means. The movement of the exit means from the closed position to the open position is controllable by said process control device.

Abstract: This relates to systems and processes for operating an automated assistant to process messages. In one example process, an electronic device receives a communication including a text string and determines whether a portion of the text string is associated with a data type of a plurality of data types. The data type is associated with at least one task. In accordance with a determination that the portion of the text string is associated with the data type, the electronic device receives a user input indicative of a task of the at least one task, and in response, causes the task to be performed based on the portion of the text string. In accordance with a determination that the portion of the text string is not associated with the data type, the electronic device foregoes causing the task to be performed based on the portion of the text string.

Abstract: The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.

Abstract: The present invention relates to a combination therapy comprising the use of riboflavin and oxaloacetate. Specifically, it is directed to new compositions, pharmaceutical compositions, kits of parts and methods for treating ischemic stroke and/or other excitotoxicity-associated diseases. It further relates to riboflavin for use in the therapeutic treatment of acute ischemic stroke by intravascular administration within the first twelve hours of symptom onset.

Abstract: A lighting device for a motor vehicle, comprising a housing in which are arranged at least light-emitting means and optical-deflection means able to deflect the rays of light emitted toward an output face of the device, the device further comprising a main orifice formed in the housing for extracting the light-emitting means from the housing along a first axis. Fastening means are provided for fastening the emitting means in the device and the housing comprises an additional axis, distinct from the main orifice, for accessing the fastening means. The invention also relates to a motor vehicle headlamp equipped with at least one device of this type, and to a method of repositioning replacement means in a lighting device.

Abstract: A motor vehicle lighting and/or signaling assembly including: a lighting module and an adapter that can be placed and retained in position in a housing of support means of an optical collector, and separate means for pressing the adapter against the support means of the module in a first direction and for adjusting the adapter in the housing of the support means in a second direction other than the first direction, these pressing and adjustment means being carried by the module and/or by the adapter. The invention further concerns the associated adapter and the associated module in this lighting and/or signaling assembly.

Abstract: Disclosed systems and methods relate to determining an intensity level of an exercise using a photoplethysmogram (PPG) sensor. A method of determining an intensity level of an exercise for a user according to one embodiment of the present disclosure includes detecting, by a device, body signals from the user using a PPG sensor. The method includes determining, by the device, a heart rate of the user based on the body signals. The method includes determining, by the device, an error in the heart rate. The method also includes determining, by the device, the intensity level of the exercise for the user based at least on the error.

Abstract: The present invention provides a system and a method for the production of cells and/or cell products. The system comprises at least one cell culture unit comprising at least one bioreactor for culturing cells, at least one technical control unit for controlling a cell growth parameters, said technical control unit is at least fluidly connected to the cell culture unit, and at least one air treatment unit for treating ambient air, said air treatment unit is fluidly connected to the cell culture unit. The system is characterized in that the system is autonomous and the bioreactor total volume is at most 1000 L.

Abstract: This relates to systems and processes for operating an automated assistant to process messages. In one example process, an electronic device receives a communication including a text string and determines whether a portion of the text string is associated with a data type of a plurality of data types. The data type is associated with at least one task. In accordance with a determination that the portion of the text string is associated with the data type, the electronic device receives a user input indicative of a task of the at least one task, and in response, causes the task to be performed based on the portion of the text string. In accordance with a determination that the portion of the text string is not associated with the data type, the electronic device foregoes causing the task to be performed based on the portion of the text string.