Abstract: The present invention provides a non-naturally occurring composition comprising a CRISPR nuclease comprising a sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 3 or a nucleic acid molecule comprising a sequence encoding the CRISPR nuclease.

Abstract: RNA molecules comprising a guide sequence portion having 17-20 nucleotides in the sequence of 17-20 contiguous nucleotides set forth in any one of SEQ ID NOs: 1-3010 and compositions, methods, and uses thereof.

Abstract: The present invention relates to a polyethylene composition comprising a) a polyethylene blend comprising from 95.5% to 99.5 wt % low density polyethylene (LDPE) and from 0.5% to 4.5 wt % high density polyethylene (HDPE), wherein the wt % (% by weight) is based the total amount of low density polyethylene and high density polyethylene in the blend, and b) a mixture of glycerol-mono-stearate and glycerol-mono-palmitate.

Abstract: A computer-implemented method may be provided for analyzing and disseminating medical information. The method may include steps performed by one or more processors including, receiving a plurality of patient medical data; aggregating the plurality of patient medical data, wherein access to patient private health information is restricted; receiving a query for medical information; analyzing the aggregated medical data based on the query; producing a result of the query based on the analyzing of the aggregated medical data; and transmitting the result of the query.

Abstract: The present invention is directed to a polyolefin resin composition comprising polyolefin and a mixture comprising glycerol ester, primary aliphatic amide and secondary aliphatic amide. The invention relates to a polyolefin foam composition obtained by foaming said polyolefin resin composition in a physical foaming process in the presence of a physical blowing agent.

Abstract: A method for analyzing and quantifying a panel of drugs in a clinical sample comprises: trapping a first portion of drug parent compounds and their metabolites on a first chromatographic column; trapping a second portion of the drug parent compounds and their metabolites on a second chromatographic column; separately eluting the first and second portions of the drug parent compounds and their metabolites from the first and second chromatographic columns; detecting concentrations of each of the drug parent compounds and metabolites eluted from each of the first and second chromatographic columns with a detector; and summing the detected concentration of each drug parent compound together with the detected concentrations of all of its respective analytes so as to derive a respective total concentration of each drug in the sample.

Abstract: The invention is directed to foamed film comprising a polyethylene foam obtained by physically foaming of a low density polyethylene copolymer with a density between 910 kg/m3 and 935 kg/m3 (according to ISO 1183) and a melt index between 0.10 and 100 dg/minute (according to ASTM D1133) wherein the low density polyethylene is obtained by a high pressure polymerization process of ethylene in the presence of a di- or higher functional monomer.

Abstract: The invention is directed to foamed caps and closure seals comprising as one of the layers polyethylene foam. The polyethylene foam is obtained by foaming of low density polyethylene copolymer obtained by a high pressure polymerization process in the presence of 1,4-butanediol dimethacrylate.

Abstract: Techniques are described for the creation and distribution of learning programs. Each learning program can include a collection of learning activities, some of which have been grouped into learning sections. The learning system can instantiate a restriction condition on a learning activity or learning section within the learning program. The restriction condition can affect when a learning activity or learning section can be executed. For example, the restriction condition can prevent a user from executing a second learning section within the learning program so long as a first learning section within the learning program is incomplete. The learning system can also credit the user for completing a learning activity even though the user has not consumed the learning activity when the learning activity is similar or the same as another learning activity which has been recently consumed.

Abstract: A computer-implemented method may be provided for analyzing and disseminating medical information. The method may include steps performed by one or more processors including, receiving a plurality of patient medical data; aggregating the plurality of patient medical data, wherein access to patient private health information is restricted; receiving a query for medical information; analyzing the aggregated medical data based on the query; producing a result of the query based on the analyzing of the aggregated medical data; and transmitting the result of the query.

Abstract: The present invention relates to the field of cancer. More specifically, the present invention provides compositions and methods for treating cancer using aqueous 32P. In certain embodiments, the present invention provides a method for treating cancer in a patient comprising the step of intravenously administering a low dose of aqueous 32P monophosphate or 32P pyrophosphate to the patient.

Abstract: A method for analyzing and quantifying a panel of drugs in a clinical sample comprises: trapping a first portion of drug parent compounds and their metabolites on a first chromatographic column; trapping a second portion of the drug parent compounds and their metabolites on a second chromatographic column; separately eluting the first and second portions of the drug parent compounds and their metabolites from the first and second chromatographic columns; detecting concentrations of each of the drug parent compounds and metabolites eluted from each of the first and second chromatographic columns with a detector; and summing the detected concentration of each drug parent compound together with the detected concentrations of all of its respective analytes so as to derive a respective total concentration of each drug in the sample.

Abstract: The present invention relates to an assembly of pipe surrounded by a multilayer oxygen barrier film wherein the multilayer oxygen barrier film comprises, in the following order, a) a layer comprising 60-100 wt % of LLDPE (linear low density polyethylene) and 0-40 wt % of LDPE (low density polyethylene), b) a first tie layer, c) a polar oxygen barrier layer, d) a second tie layer and e) a layer comprising 60-100 wt % of LLDPE and 0-40 wt % of LDPE, preferably wherein the pipe is closer to the layer e) than to the layer a).

Abstract: An apparatus comprises: a first chromatographic column fluidically coupled to a source of sample and a source of a first chromatographic mobile phase solvent; a second chromatographic column fluidically coupled to the first column; a source of a second mobile phase solvent fluidically coupled between the first and second columns; and a detector, the first chromatographic column being configurable to receive, in a trapping step, the first solvent and sample and to retain a first portion of a plurality of analytes therein and to pass a second portion of the plurality of analytes therethrough, the second chromatographic column being configurable, in the trapping step, to receive the second portion of the plurality of analytes and the first and second solvents and to retain the second portion of the plurality of analytes therein, the detector being arranged to receive the second and first portions in respective first and second elution steps.

Abstract: The invention is directed to foamed caps and closure seals comprising as one of the layers polyethylene foam. The polyethylene foam is obtained by foaming of low density polyethylene copolymer obtained by a high pressure polymerisation process in the presence of 1,4-butanediol dimethacrylate.

Abstract: The present invention relates to an assembly of pipe surrounded by a multilayer oxygen barrier film wherein the multilayer oxygen barrier film comprises, in the following order, a) a layer comprising 60-100 wt % of LLDPE (linear low density polyethylene) and 0-40 wt % of LDPE (low density polyethylene), b) a first tie layer, c) a polar oxygen barrier layer, d) a second tie layer and e) a layer comprising 60-100 wt % of LLDPE and 0-40 wt % of LDPE, preferably wherein the pipe is closer to the layer e) than to the layer a).

Abstract: The invention is directed to foamed film comprising a polyethylene foam obtained by physically foaming of a low density polyethylene copolymer with a density between 910 kg/m 3 and 935 kg/m 3 (according to ISO 1183) and a melt index between 0.10 and 100 dg/minute (according to ASTM D1133) wherein the low density polyethylene is obtained by a high pressure polymerisation process of ethylene in the presence of a di- or higher functional monomer.

Abstract: A framework for facilitating the management of, and access to, external learning content within an organization is provided. In various embodiments, a computer system (or group of computer systems) implementing the framework can, e.g.: (1) import, into the organization's learning management system (LMS), metadata for external learning content items provided by an external content provider; (2) enable an organization administrator to assign each of the external learning content items to one or more users or programs/catalogs/curricula in the organization; (3) enable organization users to search for and launch their assigned external learning content items directly from the LMS; (4) automatically log the organization users into the external content provider systems as part of the item launch process; and (5) automatically track completion of the external learning content items.

Abstract: Techniques are described for the creation and distribution of learning programs. Each learning program can include a collection of learning activities, some of which have been grouped into learning sections. The learning system can instantiate a restriction condition on a learning activity or learning section within the learning program. The restriction condition can affect when a learning activity or learning section can be executed. For example, the restriction condition can prevent a user from executing a second learning section within the learning program so long as a first learning section within the learning program is incomplete. The learning system can also credit the user for completing a learning activity even though the user has not consumed the learning activity when the learning activity is similar or the same as another learning activity which has been recently consumed.

Abstract: The invention provides certain 3,4-dihydroisoquinolin-2(1H)-yl compounds, particularly compounds of formula I, and pharmaceutical compositions thereof. The invention further provides methods of using a compound of formula I to treat cognitive impairment associated with Parkinson's disease or schizophrenia.