Abstract: Provided herein are compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular, a bioluminescent complex is formed upon the interaction of two or more peptide tags (e.g., separately or fused as a dipeptide or tripeptide) and a polypeptide component.

Abstract: A shared session cache can be accessible by multiple load balancers, and can indicate whether client devices are associated with specific backend servers of a server pool. When a client device connects to a load balancer, the load balancer can use the shared session cache to determine if the client device is already associated with a specific backend server. If so, the load balancer can connect the client device to that specific backend server so that it can continue an existing session with the specific backend server. If not, the load balancer can select a new backend server for the client device, connect the client device to the newly selected backend server, and create an entry in the shared session cache indicating that the client device is associated with the newly selected backend server.

Abstract: Provided herein are broad-spectrum G-Protein coupled receptor (GPCR) binding agents, detectable/isolatable compounds comprising such binding agents (e.g., broad-spectrum GPCR binding agents linked to a functional element and/or solid surface), and methods of use thereof for the detection/isolation of GPCRs.

Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: Provided herein are compositions and methods for stabilizing coelenterazine and analogs or derivatives thereof, and for improving the solubility and reconstitution efficiency of coelenterazine and analogs and derivatives thereof.

Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).

Abstract: A single tracking URL collects tracking information for multiple tracking services based on multiple tracking URL templates. A dynamic link generator generates a tracking URL configured to collect tracking information requested by each of a set of enabled tracking URL templates. The tracking information is transmitted to the tracking services associated with the enabled tracking URL templates. Additionally or alternatively, a tracking URL is configured to redirect a consumer to one of a candidate set of destination URLs. A tracking URL includes a base URL, which is resolved to an IP address of a server of a particular tracking service. Based on tracking data associated with the tracking URL and/or digital body language associated with the consumer, the particular tracking service selects one of a candidate set of destination URLs associated with the tracking URL. A destination page corresponding to the selected destination URL is presented to the consumer.

Abstract: Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.

Abstract: A system for opening a door of an aircraft includes a connection assembly adapted to connect the door to a fuselage section of the aircraft. The connection assembly includes a main hinge pivotally connected to the fuselage section and a link assembly connecting the door to the main hinge and pivotally connected to the fuselage section, the link assembly including one member slidingly and pivotally connected to the door. The system also includes a latch mechanism between the door and the main hinge, the latch mechanism being adapted to be actuated. The system is adapted to move the door upwardly and inwardly along a predetermined path without moving the main hinge through movement of the latch mechanism and, subsequently, to pivot the door around a virtual pivot point located outside of the aircraft by moving the main hinge once the door has reached an end of the predetermined path.

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.

Abstract: A system is presented that provides sentiment analysis technology that takes into account the perspective or context of the individual or entity for which the sentiment analysis is being performed. Using multiple points of reference within a hierarchical head noun structure (containing head nouns of root terms and possibly dependent terms), the structure allows the system to return different outcomes depending on a user query specifying one or more head nouns to be taken as reference points for sentiment calculations. As a result, an appropriate context for sentiment analysis is determined which takes into account the perspective/context associated with the individual or entity for which the analysis is performed.

Abstract: The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, or portions, components, or subunits of bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.