Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: An electronic gaming machine, including a player interface, a game display, and a game controller. The player interface is configured to receive player selections from a player. The game controller is coupled to the player interface and the game display. The game controller is configured to control the game display to present the player an option to participate in a wagering game tournament, to receive, from the player interface according to the player selections, a first selection to participate in the wagering game tournament, to control the game display to present the player an option to select from among a plurality of games for the wagering game tournament, to receive, from the player interface according to the player selections, a second selection of a tournament game from the plurality of games, and conduct the tournament game via the player interface and the game display.

Abstract: Provided are pro-substrates useful in assays of living cells. The pro-substrates can be used to detect the presence or absence of enzymes, such as luciferase, in living cells. The pro-substrates can be coelenterazine derivatives or analogs.

Abstract: Provided herein are palladium (Pd) catalysts with improved performance in biological environments. In particular, formulations, methods of preparations, and storage conditions are provided that provide improved performance of Pd catalysts under protein-rich conditions.

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: A system for opening a door of an aircraft includes a connection assembly adapted to connect the door to a fuselage section of the aircraft. The connection assembly includes a main hinge pivotally connected to the fuselage section and a link assembly connecting the door to the main hinge and pivotally connected to the fuselage section, the link assembly including one member slidingly and pivotally connected to the door. The system also includes a latch mechanism between the door and the main hinge, the latch mechanism being adapted to be actuated. The system is adapted to move the door upwardly and inwardly along a predetermined path without moving the main hinge through movement of the latch mechanism and, subsequently, to pivot the door around a virtual pivot point located outside of the aircraft by moving the main hinge once the door has reached an end of the predetermined path.

Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).

Abstract: Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Abstract: Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex).

Abstract: Provided are pro-substrates useful in assays of living cells. The pro-substrates can be used to detect the presence or absence of enzymes, such as luciferase, in living cells. The pro-substrates can be coelenterazine derivatives or analogues.

Abstract: The engineering of a pathway converting CH4 to acetate and eventually to liquid fuels is disclosed. The engineered pathway involves an engineered reversal of the natural pathway for acetate conversion to CH4 by microbes and coupling the engineered pathway to existing and future technologies for microbial or chemical conversion of acetate to liquid fuels. In one aspect, methods for producing modified pathways and/or microbes are provided. In another aspect engineered microbes, including Methanosarcina acetivorans, that incorporate the engineered pathway are provided, which can mediate conversion of CH4 to acetate for ultimate conversion to liquid fuels. In a further aspect, specific modifications to the components of the pathway are provided.

Abstract: Disclosed is an improved approach for implementing a system, method, and computer program product for integrating with a social media site, which can be used to create and configure a custom audience on the social media site.

Abstract: The present application presents an improved approach for tracking consumer digital body language. A campaign is assigned to social posts by specifying one or more campaign tags and a tracking URL template. Posts associated with a campaign are created by entering post details and parameters and automatically retrieving the campaign tags. The parameters and template may be used to form a tracking URL to be published with the post. When a consumer clicks on a URL associated with a published post, the tracking URL is read and parsed, so that the tracking information may be read and stored for analysis. In addition, digital body language may be stored for the consumer. The consumer will then be redirected to the destination URL.

Abstract: A gaming system (10) includes gaming machine groups (GR 1-GR 16) arranged to receive input data and to generate output progressive jackpot data relating to two or more progressive jackpots. A central database (26) stores the input data relating to the progressive jackpots. A monitoring computer (40) includes a local database (46). The computer (40) obtains and temporarily stores the progressive jackpot output data.