Abstract: An observation device includes: a structured illumination section; a phase difference measurement illumination section; a phase contrast lens that has a phase plate for dimming illumination light for phase difference measurement, where the illumination light for phase difference measurement is incident into the lens, and the structured illumination light is incident into the lens from a side opposite to an incidence side of the ring-shaped illumination; a detection section that detects reflected light of the structured illumination light; and an observation section that images the illumination light for phase difference measurement. When Fourier transform is performed on the structured illumination light, a spatial frequency of the structured illumination light is set on a high-frequency side or a low-frequency side with respect to a position of the phase plate on optical Fourier space.

Abstract: At least one of a stage on which a cultivation container 50 that contains a plurality of wells 52 is placed or an imaging optical system that includes an objective lens for forming an image of the observation target in each well 52 is moved to scan an observation position in the stage, to thereby observe the observation target. In a case where the objective lens is moved in an optical axis direction to perform an auto-focus control at each observation position, a start timing of the auto-focus control at each observation position D is changed on the basis of a boundary portion between respective wells 52 that are adjacent to each other in a scanning direction of the observation positions.

Abstract: An observation device includes: a structured illumination section; a phase difference measurement illumination section; a phase contrast lens that has a phase plate for dimming illumination light for phase difference measurement, where the illumination light for phase difference measurement is incident into the lens, and the structured illumination light is incident into the lens from a side opposite to an incidence side of the ring-shaped illumination; a detection section that detects reflected light of the structured illumination light; and an observation section that images the illumination light for phase difference measurement. When Fourier transform is performed on the structured illumination light, a spatial frequency of the structured illumination light is set on a high-frequency side or a low-frequency side with respect to a position of the phase plate on optical Fourier space.

Abstract: An observation device includes: a stage on which a vessel in which a subject is stored is installed; an image-forming optical system that includes an objective lens forming an image of the subject stored in the vessel; a scan control unit that moves the stage with respect to the image-forming optical system to scan each observation position in the vessel by the image-forming optical system; and an acceleration-determination-information acquisition unit that acquires at least one piece of information among information about the subject, information about the vessel, information about an observation method, or information about an observation condition. The scan control unit controls an acceleration of the stage on the basis of the at least one piece of information.

Abstract: A cell image evaluation device includes an image acquisition unit that acquires a captured image of a cell, a cell evaluation unit that evaluates the cell image, and a maturity information acquisition unit that acquires information related to maturity of the cell. The cell evaluation unit determines a method for evaluating the cell image on the basis of the information related to the maturity.

Abstract: Provided are a cell image evaluation device, method, and program which are capable of more accurate and high reliable evaluation even though a captured image of each part to be observed within a container deteriorates. The cell image evaluation device includes an image evaluation unit that evaluates a state of a cell included in a captured image obtained by capturing an inside of the container that contains the cell based on the captured image, and a deterioration determination unit that determines whether or not the captured image deteriorates. The image evaluation unit changes an evaluation method of the captured image according to a determination result of the deterioration determination unit.

Abstract: An observation image capturing and evaluation device includes an imaging unit that captures an image of a cell and acquires an observation image, an evaluation unit that evaluates the observation image, and a maturity information acquisition unit that acquires information related to the maturity of the cell. The imaging unit changes a method for capturing the observation image on the basis of the information related to the maturity.

Abstract: There are provided a microscope apparatus, an observation method, and a microscope apparatus-control program that can more efficiently perform auto-focus control and can shorten an imaging time in a case where a culture vessel is to be scanned by an image forming optical system and the auto-focus control is to be performed at each observation position. Focus information of a culture vessel is detected by a first displacement sensor and a second displacement sensor while a stage is moved to a scanning measurement position from an initial set position, and an auto-focus control unit performs auto-focus control at every observation position on the basis of the focus information in a case where the stage has been moved to the scanning measurement position.

Abstract: Disclosed are a cell evaluation device, a cell evaluation method, and a non-transitory computer readable recording medium recorded with a cell evaluation program capable of evaluating individual cells in a cell image obtained by imaging a cell group with high accuracy. The cell evaluation device includes an image acquisition unit 30 that acquires a cell image obtained by imaging a cell group; a cell evaluation unit 32 that specifies an evaluation target cell and peripheral cells around the evaluation target cell in the cell group and evaluates the evaluation target cell based on evaluation results of the peripheral cells.

Abstract: A cell culture observation device and a cell culture observation method which observe a plurality of culture containers and perform operations for each culture container capture an image without blurring caused by the operations for the culture container and improve the throughput of processing for the plurality of culture containers. The cell culture observation device includes a cell observation unit 30 that observes cells in each of a plurality of culture containers in which the cells are cultured, an operating unit 20 that performs a plurality of operations for each of the culture containers, and an operation content determination unit 41 that determines the content of an operation which can be performed for culture containers other than a culture container to be observed while the culture container to be observed is being observed among the plurality of operations.

Abstract: At least one of a stage on which a cultivation container 50 that contains a plurality of wells 52 is placed or an imaging optical system that includes an objective lens for forming an image of the observation target in each well 52 is moved to scan an observation position in the stage, to thereby observe the observation target. In a case where the objective lens is moved in an optical axis direction to perform an auto-focus control at each observation position, a start timing of the auto-focus control at each observation position D is changed on the basis of a boundary portion between respective wells 52 that are adjacent to each other in a scanning direction of the observation positions.

Abstract: Provided are a captured image evaluation apparatus, a captured image evaluation method, and a captured image evaluation program capable of evaluating a thickness and a density of stacked cultured cells in a short imaging time. The captured image evaluation apparatus includes: an image acquisition section 52 that acquires captured images obtained by imaging a subject under a condition in which a numerical aperture of an objective lens is changed; a thickness estimation section 53 that estimates a thickness of the subject on the basis of a low NA captured image obtained under a condition in which the numerical aperture of the objective lens is relatively small; and a density estimation section 54 that estimates a density of the subject on the basis of a high NA captured image obtained under a condition in which the numerical aperture of the objective lens is relatively large.

Abstract: Disclosed are a cell determination device, a cell determination method, and a non-transitory computer readable recording medium recorded with a cell determination program capable of objectively determining a state of a cell with high accuracy. The cell determination device includes: a cell information acquisition unit 31 that acquires information relating to a proliferation rate of a cell and information relating to a movement distance of the cell per unit time based on plural cell images obtained by imaging the cell in a time series manner; and a determination unit 32 that determines a state of the cell based on the information relating to the proliferation rate and the information relating to the movement distance.

Abstract: A nucleolus detection unit, which detects nucleoli in a plurality of cells in a cell image obtained by imaging the cells, and a cell recognition unit, which acquires information indicating a distance between the nucleoli and recognizes the individual cells based on the information indicating the distance, are provided.

Abstract: Provided is a cell analysis system that can keep only a target cell fresh. A cell analysis system 1 includes: a detection device 10 that detects staining intensity of a stained cell; a collection container 20 that collects a plurality of cells whose staining intensity has been detected by the detection device 10; a control unit 40 that determines priority in acquiring image data on the basis of a detection result of the staining intensity of the stained cell; and an imaging apparatus 30 that acquires the image data of the cells collected into the collection container 20 on the basis of the priority determined by the control unit 40.

Abstract: Provided are a robust and high throughput method for identifying a target cell and a target cell identification device. The method for identifying a target cell includes an image acquisition step of irradiating a specimen with a plurality of light rays having different wavelengths and acquiring a plurality of phase difference images of the specimen, a selection step of selecting a plurality of target cell candidates from the specimen based on a difference in absorption coefficients with respect to the plurality of light rays having different wavelengths, a brightness ratio acquisition step of acquiring brightness ratios between a central part and a peripheral part of each target cell candidate based on the plurality of phase difference images, and a sorting step of sorting a target cell and a non-target cell from the plurality of target cell candidates based on the brightness ratios.

Abstract: The present invention provides a cell evaluation device, a cell evaluation method and a non-transitory computer readable recording medium storing a cell evaluation program capable of evaluating an evaluation target cell. The cell evaluation device includes an image acquisition unit that acquires a cell image obtained by imaging a cell group; a cell evaluation unit that specifies an evaluation target cell and peripheral cells around the evaluation target cell in the cell group, and evaluates the evaluation target cell based on evaluation results of the peripheral cells; and a boundary setting unit that sets a boundary in the cell image based on a state of the cell group, in which when specifying the peripheral cells, the cell evaluation unit specifies only cells that are present in a divided region where the evaluation target cell is present among plural divided regions divided by the boundary, as the peripheral cells.

Abstract: A cell observation apparatus includes an image formation optical system control unit 51 which sets an initial scanning range of the focal position based on information relating to a thickness of a cell, forms an image of the cell at each of a plurality of focal positions within a set initial scanning range, subsequently acquires an image captured by an imaging unit 40 for each of the plurality of focal positions, estimates the thickness of the cell based on the image, updates the initial scanning range of the focal position based on the estimated thickness of the cell, and forms an image of the cell at each of a plurality of focal positions within the updated scanning range.

Abstract: A microscope includes an illumination light emission unit 10 that emits illumination light, a stage 61 on which a culture container 60 is placed, an objective lens 31 on which the illumination light having passed through the culture container 60 and the stage 61 is incident, a focusing light emission unit 70 that emits focusing light, a reflected light detection unit 75 that detects reflected light due to emission of the focusing light, a distance changing unit 34 that changes a distance between the objective lens 31 and the stage 61, an autofocus control unit 51 that performs autofocus control based on the reflected light, and a focus control information acquisition unit 53 that acquires focus control information including information of the culture container 60, the amount of culture solution C, and the magnification of the objective lens 31.

Abstract: An observation image determination device includes an observation image acquisition unit that captures an image of an observation region including a stem cell to be cultured to acquire an observation image and a determination unit that determines whether a living body of a different type from the stem cell is included in the observation region. The determination unit determines whether the different type of living body is included in the observation region, on the basis of at least one of form information of an observation target and information about a change in the observation target over time which are acquired from the observation image.