Abstract: A fireworks kit can have a set of fireworks having multiple fireworks of different types and a plurality of detonators in communication with the fireworks that are configured to launch or detonate a firework attached thereto. A remote controller or mobile device can be in communication with the detonators and operable to provide dynamic or user customizable control of detonation, launching, or ignition of the fireworks.

Abstract: An acrylamide gel formulation for use in electrophoresis, and a system and method for electrophoretic gel casting. In some embodiments, the system comprises a UV-Vis curing light source, and an acrylamide formulation that allows a resolving gel and a stacking gel to be photopolymerized in a single step. In some embodiments, the resolving solution formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethylbenzoyl-phosphinate (LAP) as a water-soluble photoinitiator, which allows the solution to be photopolymerized using UV radiation within a certain wavelength. In some embodiments, a dilution buffer formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethyl-benzoyl-phosphinate (LAP). The dilution buffer may be added to the resolving solution in a selected amount in order to modify the concentration of the acrylamide to a desired concentration.

Abstract: The disclosed embodiments concern methods, systems and computer program products for determining sequences of interest using unique molecular indexes (UMIs) that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies or long sequence length. In some implementations, the UMIs include variable-length nonrandom UMIs (vNRUMIs). Methods and systems for making and using sequencing adapters comprising vNRUMIs are also provided.

Abstract: Various embodiments of the present disclosure provide techniques for optimally augmenting a training dataset for a machine learning model based on multiple model-focused predictions. The techniques may include generating a datapoint priority matrix that corresponds to a plurality of entity-feature value pairs of a training dataset for a machine learning model, generating a plurality of impact predictions and feature sensitivity predictions for the plurality of entity-feature value pairs, generating a refined datapoint priority matrix by updating the datapoint priority matrix based on the plurality of impact predictions and sensitivity predictions, and providing a datapoint collection output for the training dataset based on the refined datapoint priority matrix and a data augmentation threshold.

Abstract: A fireworks kit can have a set of fireworks having multiple fireworks of different types and a plurality of detonators in communication with the fireworks that are configured to launch or detonate a firework attached thereto. A remote controller or mobile device can be in communication with the detonators and operable to provide dynamic or user customizable control of detonation, launching, or ignition of the fireworks.

Abstract: Methods for screening clonotypes are provided. Data representing a plurality of cells from a single subject is obtained. The data represents a plurality of clonotypes. The data includes a plurality of contigs for each respective clonotype in the plurality of clonotypes. Each respective contig in the plurality of contigs comprises (i) an indication of chain type for the respective contig and (ii) a contig sequence of an mRNA of the respective cell. There is determined, using the data, for each respective clonotype in the plurality of clonotypes, a number of the plurality of cells that represent the respective clonotype. In some instances, more than one cell in the plurality of cells have the same clonotype in the plurality of clonotypes. In some instances, the plurality of clonotypes comprises 25 clonotypes and where the plurality of cells includes at least one cell for each clonotype in the plurality of clonotypes.

Abstract: Utilization data of reserved virtual machine (VM) instances can be obtained from a cloud service provider. A refund recommendation comprising a first quantity of VM instances and a refund credit associated with the first quantity of VM instances can be generated based on the utilization data. A purchase recommendation comprising a second quantity of VM instances and a purchase cost associated with the second quantity of VM instances can be generated. An exchange transaction to return the first quantity of VM instances and purchase the second quantity of VM instances can be generated, where a net total of the exchange transaction is determined based on the refund credit and the purchase cost.

Abstract: The disclosed embodiments concern methods, systems and computer program products for determining sequences of interest using unique molecular indexes (UMIs) that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies or long sequence length. In some implementations, the UMIs include variable-length nonrandom UMIs (vNRUMIs). Methods and systems for making and using sequencing adapters comprising vNRUMIs are also provided.

Abstract: Methods for screening clonotypes are provided. Data representing a plurality of cells from a single subject is obtained. The data represents a plurality of clonotypes. The data includes a plurality of contigs for each respective clonotype in the plurality of clonotypes. Each respective contig in the plurality of contigs comprises (i) an indication of chain type for the respective contig and (ii) a contig sequence of an mRNA of the respective cell. There is determined, using the data, for each respective clonotype in the plurality of clonotypes, a number of the plurality of cells that represent the respective clonotype. In some instances, more than one cell in the plurality of cells have the same clonotype in the plurality of clonotypes. In some instances, the plurality of clonotypes comprises 25 clonotypes and where the plurality of cells includes at least one cell for each clonotype in the plurality of clonotypes.

Abstract: The disclosed embodiments concern methods, apparatus, systems and computer program products for determining sequences of interest using unique molecular index sequences that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies and long sequence length. In some implementations, the unique molecular index sequences include variable-length nonrandom sequences. In some implementations, the unique molecular index sequences are associated with the individual polynucleotide fragments based on alignment scores indicating similarity between the unique molecular index sequences and subsequences of sequence reads obtained from the individual polynucleotide fragments. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

Abstract: Methods and compositions for preparing and priming a tissue graft for an accelerated therapeutic effect are provided herein. In one embodiment, the method includes obtaining a tissue containing viable cells from a donor, wherein the viable cells are endogenous to the tissue and remain resident in the tissue; and priming the viable cells with one or more stimuli comprising simulated hypoxia to produce a primed tissue, wherein when grafted to a recipient the primed tissue provides a benefit compared to non-primed tissue.

Abstract: The disclosed embodiments concern methods, apparatus, systems and computer program products for determining sequences of interest using unique molecular index sequences that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies and long sequence length. In some implementations, the unique molecular index sequences include variable-length nonrandom sequences. In some implementations, the unique molecular index sequences are associated with the individual polynucleotide fragments based on alignment scores indicating similarity between the unique molecular index sequences and subsequences of sequence reads obtained from the individual polynucleotide fragments. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

Abstract: Logic may identify feature contributions to erroneous predictions by predictive models. Logic may provide a set of two or more models. Each model may train based on a training dataset and test based on a testing dataset and two or more models may be unique. Logic may test the set during a monitoring period. Logic may perform residual modeling on each model in the set during the monitoring period and may determine a list of input features that contribute to a residual of each model of the set. A residual comprises a difference between a predicted result and an expected result. Logic may generate a combined list of the input features from the set and may rank the input features. Logic may perform a voting process to generate the ranks for the input features. And logic may classify features as exogenous or endogenous based on a threshold and the ranks.

Abstract: A fireworks kit can have a set of fireworks having multiple fireworks of different types and a plurality of detonators in communication with the fireworks that are configured to launch or detonate a firework attached thereto. A remote controller or mobile device can be in communication with the detonators and operable to provide dynamic or user customizable control of detonation, launching, or ignition of the fireworks.

Abstract: The disclosed embodiments concern methods, systems and computer program products for determining sequences of interest using unique molecular indexes (UMIs) that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies or long sequence length. In some implementations, the UMIs include variable-length nonrandom UMIs (vNRUMIs). Methods and systems for making and using sequencing adapters comprising vNRUMIs are also provided.