Abstract: The disclosed embodiments concern methods, systems and computer program products for determining sequences of interest using unique molecular indexes (UMIs) that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies or long sequence length. In some implementations, the UMIs include variable-length nonrandom UMIs (vNRUMIs). Methods and systems for making and using sequencing adapters comprising vNRUMIs are also provided.

Abstract: Tissue derived porous matrices for treating wounds are provided, as well as methods for making and using them. The tissue derived porous matrices comprise processed tissue of any of several types, such as dermis, adipose, etc., and have a plurality of interconnected pores which allow fluid flow through the matrices. The tissue derived matrices are biocompatible resorbable matrices which remodel with native tissue and facilitate and enhance cell infiltration and tissue ingrowth into the matrices during the wound healing process, thereby enhancing wound healing and tissue remodeling when implanted into a patient. The tissue derived matrices are useful with reduced or negative pressure wound healing methods and systems, without the need to repeatedly revisit the treatment site and remove previously implanted matrices.

Abstract: A tissue graft container is provided having an interior space and including a vent which allows fluid (liquid and vapor) to escape from the container during drying of the tissue graft. The vent may be one or more apertures, one or more elongated openings, or a combination thereof, through one or more components of the container. A tissue graft packaging system having a retainer including first and second members with respective first and second pluralities of channels, and configured to removeably interconnect the first and second members in a closed configuration. The first and second pluralities of channels create a continuous interior space. The retainer further includes a vent, such as apertures, slits, etc., through one or both of the first and second retainers, for allowing moisture to escape from the retainer during the drying of a tissue graft contained within the continuous interior space of the retainer.

Abstract: Logic may identify feature contributions to erroneous predictions by predictive models. Logic may provide a set of two or more models. Each model may train based on a training dataset and test based on a testing dataset and two or more models may be unique. Logic may test the set during a monitoring period. Logic may perform residual modeling on each model in the set during the monitoring period and may determine a list of input features that contribute to a residual of each model of the set. A residual comprises a difference between a predicted result and an expected result. Logic may generate a combined list of the input features from the set and may rank the input features. Logic may perform a voting process to generate the ranks for the input features. And logic may classify features as exogenous or endogenous based on a threshold and the ranks.

Abstract: Logic may identify feature contributions to erroneous predictions by predictive models. Logic may provide a set of two or more models. Each model may train based on a training dataset and test based on a testing dataset and two or more models may be unique. Logic may test the set during a monitoring period. Logic may perform residual modeling on each model in the set during the monitoring period and may determine a list of input features that contribute to a residual of each model of the set. A residual comprises a difference between a predicted result and an expected result. Logic may generate a combined list of the input features from the set and may rank the input features. Logic may perform a voting process to generate the ranks for the input features. And logic may classify features as exogenous or endogenous based on a threshold and the ranks.

Abstract: A cutting device is provided having multiple evenly-spaced cutting blades and which is useful for producing tissue forms comprising membranous tissue fragments which are relatively uniformly sized and shaped. The present invention also relates to methods for using the cutter device to quickly and efficiently produce such tissue forms which are useful as grafts and can be flowable and easily passable through a luer-slip tip syringe or needle.

Abstract: Methods and compositions for preparing and priming a tissue graft for an accelerated therapeutic effect are provided herein. In one embodiment, the method includes obtaining a tissue containing viable cells from a donor, wherein the viable cells are endogenous to the tissue and remain resident in the tissue; and priming the viable cells with one or more stimuli comprising simulated hypoxia to produce a primed tissue, wherein when grafted to a recipient the primed tissue provides a benefit compared to non-primed tissue.

Abstract: A fireworks kit can have a set of fireworks having multiple fireworks of different types and a plurality of detonators in communication with the fireworks that are configured to launch or detonate a firework attached thereto. A remote controller or mobile device can be in communication with the detonators and operable to provide dynamic or user customizable control of detonation, launching, or ignition of the fireworks.

Abstract: A detonation wire has a firing module connector at one end and a firework connection assembly at another end. The firework connection assembly has a connector housing having a plurality of sections at least partially surrounding and defining an ignition chamber. The firework connection assembly also has one or more firework igniters positioned at least partially within the ignition chamber. A firework igniter is operable to activate an ignition element of a respective firework placed into engagement therewith. Other examples also are described.

Abstract: Methods and compositions for preparing and priming a tissue graft for an accelerated therapeutic effect are provided herein. In one embodiment, the method includes obtaining a tissue containing viable cells from a donor, wherein the viable cells are endogenous to the tissue and remain resident in the tissue; and priming the viable cells with one or more stimuli to produce a primed tissue, wherein when grafted to a recipient the primed tissue provides a benefit compared to non-primed tissue.

Abstract: The disclosed embodiments concern methods, apparatus, systems and computer program products for determining sequences of interest using unique molecular index sequences that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies and long sequence length. In some implementations, the unique molecular index sequences include variable-length nonrandom sequences. In some implementations, the unique molecular index sequences are associated with the individual polynucleotide fragments based on alignment scores indicating similarity between the unique molecular index sequences and subsequences of sequence reads obtained from the individual polynucleotide fragments. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

Abstract: A fireworks kit can have a set of fireworks having multiple fireworks of different types and a plurality of detonators in communication with the fireworks that are configured to launch or detonate a firework attached thereto. A remote controller or mobile device can be in communication with the detonators and operable to provide dynamic or user customizable control of detonation, launching, or ignition of the fireworks.

Abstract: The present invention relates generally to tissue forms useful as grafts or implants and derived from membranous tissue. The tissue forms may contain viable endogenous cells. The tissue forms may be sheets or mini sheets, each having quadrilateral, circular, polygonal, or irregular shapes. The sheet tissue form may have a generally quadrilateral shape, with an average length of about 1 to about 20 centimeters and an average width of about 1 to about 20 centimeters. The mini sheet tissue form may have a generally quadrilateral shape, with an average length of about 0.5 to about 9 millimeters and an average width of about 0.5 to about 9 millimeters. A mixture of the mini sheet tissue form and a liquid, such as cryopreservation fluid, saline or buffer solution, is flowable, which means the mixture will pass through a luer-slip tip syringe, or a gauge needle.

Abstract: The disclosed embodiments concern methods, apparatus, systems and computer program products for determining sequences of interest using unique molecular index sequences that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies and long sequence length. In some implementations, the unique molecular index sequences include variable-length nonrandom sequences. In some implementations, the unique molecular index sequences are associated with the individual polynucleotide fragments based on alignment scores indicating similarity between the unique molecular index sequences and subsequences of sequence reads obtained from the individual polynucleotide fragments. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

Abstract: A tissue graft container is provided having an interior space and including a vent which allows fluid (liquid and vapor) to escape from the container during drying of the tissue graft. The vent may be one or more apertures, one or more elongated openings, or a combination thereof, through one or more components of the container. A tissue graft packaging system having a retainer including first and second members with respective first and second pluralities of channels, and configured to removeably interconnect the first and second members in a closed configuration. The first and second pluralities of channels create a continuous interior space. The retainer further includes a vent, such as apertures, slits, etc., through one or both of the first and second retainers, for allowing moisture to escape from the retainer during the drying of a tissue graft contained within the continuous interior space of the retainer.

Abstract: A fireworks kit can have a set of fireworks having multiple fireworks of different types and a plurality of detonators in communication with the fireworks that are configured to launch or detonate a firework attached thereto. A remote controller or mobile device can be in communication with the detonators and operable to provide dynamic or user customizable control of detonation, launching, or ignition of the fireworks.

Abstract: A tissue graft packaging system having a retainer including first and second members with respective first and second pluralities of channel sidewalls that define first and second pluralities of channels, and first and second engagement means configured to removeably interconnect the first and second members. The first and second pluralities of channel sidewalls are configured to create a continuous interior space between the first and second members. A tissue graft, such as a cryopreserved viable tissue graft, is contained within the continuous interior space of the retainer. Processes for packaging, thawing and rinsing the tissue graft using the tissue graft packaging system and its retainer are also disclosed.

Abstract: Logic may identify feature contributions to erroneous predictions by predictive models. Logic may provide a set of two or more models. Each model may train based on a training dataset and test based on a testing dataset and two or more models may be unique. Logic may test the set during a monitoring period. Logic may perform residual modeling on each model in the set during the monitoring period and may determine a list of input features that contribute to a residual of each model of the set. A residual comprises a difference between a predicted result and an expected result. Logic may generate a combined list of the input features from the set and may rank the input features. Logic may perform a voting process to generate the ranks for the input features. And logic may classify features as exogenous or endogenous based on a threshold and the ranks.