Patents by Inventor Koji Tsujita

Koji Tsujita has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10962531
    Abstract: A first sample solution including exosomes including first to third detection target substances is mixed with a first buffer solution including first nanoparticles including first binding substances which bind to the first detection target substances. The first detection target substances and the first binding substances are bound together, so as to form first complexes of the exosomes and the first nanoparticles. The first complexes are isolated from a mixed solution of the first sample solution and the first buffer solution. The second detection target substances and the second binding substances are bound together, so as to capture the first complexes on a substrate. The second binding substances are fixed onto the substrate. A second buffer solution including second nanoparticles including third binding substances which bind to the third detection target substances is reacted with the first complexes.
    Type: Grant
    Filed: February 15, 2018
    Date of Patent: March 30, 2021
    Assignee: JVC KENWOOD CORPORATION
    Inventors: Makoto Itonaga, Yuichi Hasegawa, Koji Tsujita, Masayuki Ono, Shigehiko Iwama, Makoto Igarashi
  • Patent number: 10866233
    Abstract: An analysis device includes a turntable, an optical pickup, and a controller. The turntable holds a specimen analysis disc having reaction regions on which nanoparticles binding to substances to be detected are captured. The optical pickup emits laser light to each reaction region, receives a reflected light from each reaction region, and generates a light reception level signal. The controller sequentially generates a plurality of measurement gate signals for counting the number of the nanoparticles captured on each reaction region, counts the number of the nanoparticles of each of the measurement gate signals based on the light reception level signal, specifies a measurement gate section in each reaction region according to a measurement result per measurement gate signal, and adds up the number of the nanoparticles of the respective measurement gate signals in the measurement gate section.
    Type: Grant
    Filed: July 13, 2018
    Date of Patent: December 15, 2020
    Assignee: JVC KENWOOD CORPORATION
    Inventors: Shigehiko Iwama, Makoto Itonaga, Yuichi Hasegawa, Koji Tsujita, Masayuki Ono
  • Publication number: 20200284784
    Abstract: An analysis unit for quantitating detection target substances bound to antibodies includes wells and inclination parts. The wells each have a hole-like shape defined by an opening, an inner circumferential surface, and a bottom. The inclination parts each have an inclined surface connected to the inner circumferential surface and inclined downward such that whose height with respect to the bottom decreases as a distance from an outer circumferential side of the well increases.
    Type: Application
    Filed: February 21, 2020
    Publication date: September 10, 2020
    Inventors: Shigehiko IWAMA, Yuichi HASEGAWA, Koji TSUJITA, Masayuki ONO, Katsue HORIKOSHI, Atsushi SAITO
  • Publication number: 20200217864
    Abstract: The dispensing unit includes a disc, a cartridge, and a dispensing holder. The disc has a disc shape and includes a track region provided with recesses and projections alternately arranged in a radial direction. The cartridge includes a penetration hole, and a well is formed by the penetration hole and the track region in a state in which the cartridge is attached to the disc. The dispensing holder includes a holding part to be inserted into the penetration hole, and a guide hole penetrating the holding part. The guide hole has a truncated cone shape in which a first opening diameter on the holding part side is smaller than a second opening diameter on a side opposite to the holding part, and a center line of the guide hole is located on a line passing through the center of the disc and the center of the well.
    Type: Application
    Filed: March 13, 2020
    Publication date: July 9, 2020
    Inventors: Katsue HORIKOSHI, Makoto ITONAGA, Koji TSUJITA, Masayuki ONO, Yuichi HASEGAWA
  • Publication number: 20200173918
    Abstract: An analysis substrate is irradiated with laser light, and reflected light from reaction region is received to generate a light reception level signal. Particle detection signal having a signal level higher than a predetermined signal level is extracted from the light reception level signal in the reaction region, so as to detect detection target substance in accordance with the extracted particle detection signal. The analysis substrate has the reaction region on which the detection target substance, primary particle provided with antibody for labeling the detection target substance, and secondary particle formed of metal and provided with antigen to be bound to the antibody are captured.
    Type: Application
    Filed: February 4, 2020
    Publication date: June 4, 2020
    Inventors: Makoto ITONAGA, Masayuki ONO, Yuichi HASEGAWA, Koji TSUJITA
  • Patent number: 10629237
    Abstract: A magnet structure includes first and second magnet units. The first magnet unit is provided with a first magnet fixing section that includes a first surface and a first magnet having a first polarity on the first surface's side. The second magnet unit is provided with a second magnet fixing section that includes a second surface and a second magnet having a second polarity on the second surface's side, the second polarity being an opposite polarity to the first polarity. The first and second surfaces are located next to one another on the same plane to form a magnetic attachment surface which is magnetically attached to a magnetically-attached object. The second magnet unit is supported by the first magnet unit so as to move in a predetermined range.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: April 21, 2020
    Assignee: JVC KENWOOD CORPORATION
    Inventors: Shigehiko Iwama, Makoto Itonaga, Yuichi Hasegawa, Koji Tsujita, Masayuki Ono, Katsue Horikoshi
  • Patent number: 10627398
    Abstract: An analysis device optically scans a surface of a substrate to which particles are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the particles based on pulse interval between two pulse waves each having pulse width less than first reference value determined depending on first pulse width when the optical scanning unit scans a plurality of particles adjacent to each other when the two pulse waves are detected consecutively.
    Type: Grant
    Filed: May 15, 2019
    Date of Patent: April 21, 2020
    Assignee: JVC KENWOOD CORPORATION
    Inventors: Masayuki Ono, Shingo Yagyu, Makoto Itonaga, Yuichi Hasegawa, Koji Tsujita
  • Publication number: 20200088626
    Abstract: An analysis device optically scans a surface of a substrate to which analytes and particles for labeling the analytes are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the analytes and determines that the analyte count is one when two pulse waves are detected consecutively each having pulse width less than first reference value determined depending on first pulse width in the detection signal when the optical scanning unit scans a plurality of particles adjacent to each other.
    Type: Application
    Filed: November 25, 2019
    Publication date: March 19, 2020
    Inventors: Masayuki ONO, Shingo YAGYU, Makoto ITONAGA, Yuichi HASEGAWA, Koji TSUJITA
  • Patent number: 10520417
    Abstract: An analysis method irradiates, with laser light, an analysis substrate made of a resin material and having a reaction region on which detection target substances and nanoparticles of a metal compound for labeling the detection target substances are captured. The analysis method extracts, as a substrate signal level, a signal level generated when receiving reflected light from the analysis substrate. The analysis method receives reflected light from the reaction region to generate a light reception level signal. The analysis method extracts a nanoparticle detection signal from the light reception level signal of the reflected light from the reaction region, the nanoparticle detection signal having a higher level than the signal level of the reflected light from the analysis substrate. The analysis method detects the nanoparticles in accordance with the extracted nanoparticle detection signal.
    Type: Grant
    Filed: October 31, 2018
    Date of Patent: December 31, 2019
    Assignee: JVC KENWOOD CORPORATION
    Inventors: Masayuki Ono, Makoto Itonaga, Yuichi Hasegawa, Koji Tsujita, Shigehiko Iwama
  • Publication number: 20190265237
    Abstract: An analysis device optically scans a surface of a substrate to which particles are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the particles based on pulse interval between two pulse waves each having pulse width less than first reference value determined depending on first pulse width when the optical scanning unit scans a plurality of particles adjacent to each other when the two pulse waves are detected consecutively.
    Type: Application
    Filed: May 15, 2019
    Publication date: August 29, 2019
    Inventors: Masayuki ONO, Shingo YAGYU, Makoto ITONAGA, Yuichi HASEGAWA, Koji TSUJITA
  • Publication number: 20190206435
    Abstract: A magnet structure includes first and second magnet units. The first magnet unit is provided with a first magnet fixing section that includes a first surface and a first magnet having a first polarity on the first surface's side. The second magnet unit is provided with a second magnet fixing section that includes a second surface and a second magnet having a second polarity on the second surface's side, the second polarity being an opposite polarity to the first polarity. The first and second surfaces are located next to one another on the same plane to form a magnetic attachment surface which is magnetically attached to a magnetically-attached object. The second magnet unit is supported by the first magnet unit so as to move in a predetermined range.
    Type: Application
    Filed: March 6, 2019
    Publication date: July 4, 2019
    Inventors: Shigehiko IWAMA, Makoto ITONAGA, Yuichi HASEGAWA, Koji TSUJITA, Masayuki ONO, Katsue HORIKOSHI
  • Publication number: 20190064048
    Abstract: An analysis method irradiates, with laser light, an analysis substrate made of a resin material and having a reaction region on which detection target substances and nanoparticles of a metal compound for labeling the detection target substances are captured. The analysis method extracts, as a substrate signal level, a signal level generated when receiving reflected light from the analysis substrate. The analysis method receives reflected light from the reaction region to generate a light reception level signal. The analysis method extracts a nanoparticle detection signal from the light reception level signal of the reflected light from the reaction region, the nanoparticle detection signal having a higher level than the signal level of the reflected light from the analysis substrate. The analysis method detects the nanoparticles in accordance with the extracted nanoparticle detection signal.
    Type: Application
    Filed: October 31, 2018
    Publication date: February 28, 2019
    Inventors: Masayuki ONO, Makoto ITONAGA, Yuichi HASEGAWA, Koji TSUJITA, Shigehiko IWAMA
  • Publication number: 20180321227
    Abstract: An analysis device includes a turntable, an optical pickup, and a controller. The turntable holds a specimen analysis disc having reaction regions on which nanoparticles binding to substances to be detected are captured. The optical pickup emits laser light to each reaction region, receives a reflected light from each reaction region, and generates a light reception level signal. The controller sequentially generates a plurality of measurement gate signals for counting the number of the nanoparticles captured on each reaction region, counts the number of the nanoparticles of each of the measurement gate signals based on the light reception level signal, specifies a measurement gate section in each reaction region according to a measurement result per measurement gate signal, and adds up the number of the nanoparticles of the respective measurement gate signals in the measurement gate section.
    Type: Application
    Filed: July 13, 2018
    Publication date: November 8, 2018
    Inventors: Shigehiko IWAMA, Makoto ITONAGA, Yuichi HASEGAWA, Koji TSUJITA, Masayuki ONO
  • Patent number: 10119962
    Abstract: A recessed portion and a protruding portion arranged periodically are formed on a base portion. In the recessed portion, an antibody that binds to an antigen existing on a surface of each exosome to be detected is immobilized and then caused to bind to the exosomes. The width of the protruding portion is smaller than the average particle diameter of the exosomes.
    Type: Grant
    Filed: October 5, 2015
    Date of Patent: November 6, 2018
    Assignee: JVC KENWOOD CORPORATION
    Inventors: Makoto Itonaga, Shingo Yagyu, Yuichi Hasegawa, Koji Tsujita, Masayuki Ono
  • Publication number: 20180217175
    Abstract: An analysis device includes a turntable holding a substrate, an optical pickup driven in a direction perpendicular to a rotation axis of the turntable and configured to emit laser light to reaction regions and to receive reflected light from the respective reaction regions, an optical pickup drive circuit, and a controller. The reaction regions are formed at positions different from the center of the substrate. The center of the substrate is located on the rotation axis of the turntable. The optical pickup detects a reception level of the reflected light to generate a light reception level signal. The controller controls a turntable drive circuit to rotate the substrate, controls the optical pickup drive circuit to drive the optical pickup, and specifies the respective reaction regions in accordance with a positional information signal and the light reception level signal.
    Type: Application
    Filed: March 28, 2018
    Publication date: August 2, 2018
    Inventors: Shigehiko IWAMA, Makoto ITONAGA, Yuichi HASEGAWA, Koji TSUJITA, Masayuki ONO, Makoto IGARASHI
  • Publication number: 20180180604
    Abstract: A first sample solution including exosomes including first to third detection target substances is mixed with a first buffer solution including first nanoparticles including first binding substances which bind to the first detection target substances. The first detection target substances and the first binding substances are bound together, so as to form first complexes of the exosomes and the first nanoparticles. The first complexes are isolated from a mixed solution of the first sample solution and the first buffer solution. The second detection target substances and the second binding substances are bound together, so as to capture the first complexes on a substrate. The second binding substances are fixed onto the substrate. A second buffer solution including second nanoparticles including third binding substances which bind to the third detection target substances is reacted with the first complexes.
    Type: Application
    Filed: February 15, 2018
    Publication date: June 28, 2018
    Inventors: Makoto ITONAGA, Yuichi HASEGAWA, Koji TSUJITA, Masayuki ONO, Shigehiko IWAMA, Makoto IGARASHI
  • Publication number: 20170184582
    Abstract: An analysis device includes an optical scanning unit and a pulse waveform analysis unit. The optical scanning unit optically scans a surface of a substrate to which analytes binding to particles are fixed, and obtains a detection signal from the substrate. The pulse waveform analysis unit includes an amplitude determination unit configured to determine whether a peak value of the pulse waveform is within a first range smaller than a first level and greater than a second level having a polarity identical to the first level; and a pulse width determination unit configured to determine whether a pulse width of the pulse waveform is within a second range.
    Type: Application
    Filed: March 16, 2017
    Publication date: June 29, 2017
    Inventors: Masayuki ONO, Shingo YAGYU, Makoto ITONAGA, Yuichi HASEGAWA, Koji TSUJITA
  • Publication number: 20170010260
    Abstract: An analysis device optically scans a surface of a substrate to which particles are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the particles based on pulse interval between two pulse waves each having pulse width less than first reference value determined depending on first pulse width when the optical scanning unit scans a plurality of particles adjacent to each other when the two pulse waves are detected consecutively.
    Type: Application
    Filed: September 21, 2016
    Publication date: January 12, 2017
    Inventors: Masayuki ONO, Shingo YAGYU, Makoto ITONAGA, Yuichi HASEGAWA, Koji TSUJITA
  • Publication number: 20170003213
    Abstract: An analysis device optically scans a surface of a substrate to which analytes and particles for labeling the analytes are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the analytes and determines that the analyte count is one when two pulse waves are detected consecutively each having pulse width less than first reference value determined depending on first pulse width in the detection signal when the optical scanning unit scans a plurality of particles adjacent to each other.
    Type: Application
    Filed: September 19, 2016
    Publication date: January 5, 2017
    Inventors: Masayuki ONO, Shingo YAGYU, Makoto ITONAGA, Yuichi HASEGAWA, Koji TSUJITA
  • Publication number: 20160033486
    Abstract: A recessed portion and a protruding portion arranged periodically are formed on a base portion. In the recessed portion, an antibody that binds to an antigen existing on a surface of each exosome to be detected is immobilized and then caused to bind to the exosomes. The width of the protruding portion is smaller than the average particle diameter of the exosomes.
    Type: Application
    Filed: October 5, 2015
    Publication date: February 4, 2016
    Inventors: Makoto ITONAGA, Shingo YAGYU, Yuichi HASEGAWA, Koji TSUJITA, Masayuki ONO