Abstract: A manufacturing method of silatrane with thiol group and a preservation method thereof are disclosed. (3-Mercaptopropyl)trimethoxysilane and triethanolamine are reacted for a pre-determined time at a pre-determined temperature under nitrogen atmosphere in presence or absence of catalyst, and then a recrystallization process is performed with a solvent to obtain silatrane with thiol group of formula (1). Silatrane with thiol group is dissolved in an organic solvent to preserve the silatrane with thiol group.

Abstract: A reflection-based tubular waveguide particle plasmon resonance sensing system and a sensing device thereof are provided. The sensing device includes a hollow tubular waveguide element having wall, a reflection layer disposed on one end of the wall (distal end), and a noble metal nanoparticle layer distributed on the surface of the wall. An incident light enters the wall through another end of the tubular waveguide element (proximal end) and being total internal reflected many times along the wall, then is reflected by the reflection layer and being total internal reflected many times along the wall again, and finally, the incident light exits the proximal end. Wherein, when the sample contacts the noble metal nanoparticle layer of the tubular waveguide element, the particle plasmon resonance condition is altered and hence the signal intensity of the light exiting the tubular waveguide element changes.

Abstract: A method for fixing metal onto a surface of the substrate. The present method includes steps of: providing a substrate and a mercaptoalkylsilatrane compound; dissolving the mercaptoalkylsilatrane compound in a solvent; performing a condensation reaction of the substrate with and the dissolved mercaptoalkylsilatrane compound to complete the surface modification of the substrate; and performing a covalent bonding process to metal with the mercaptoalkylsilatrane compound already modified onto the surface of the substrate to fix the metal onto the surface of the substrate.

Abstract: A self-referencing localized plasmon resonance sensing device and a system thereof are disclosed. The reference optical waveguide element is modified with a noble metal nanoparticle layer. The sensing optical waveguide element is modified with a noble metal nanoparticle layer, which is further modified with a recognition unit. The incident light is guided into the reference and the sensing optical waveguide elements to respectively generate localized plasmon resonance sensor signals. The reference and the sensing optical waveguide elements respectively have a calibration slope. The processor utilizes the calibration slopes to regulate the second difference generated by detecting with the sensing optical waveguide element. The processor utilizes a difference between the first difference, which is generated by detecting with the reference optical waveguide element, and the regulated second difference to obtain a sensor response.

Abstract: Disclosed is a microfluidic biosensing system including a processor, in which a Raman barcode database corresponding to at least one Raman spectrum signal is stored, a plurality of Raman barcode beads mixed with a target fluid and coupled to at least one target bioparticle in the target fluid, a microfluidic channel disposed to make the target fluid mixed with the Raman barcode beads flow therethrough, a light source disposed on the microfluidic channel, and a spectral detection device connected to the processor and disposed to correspond to the light source. The spectral detection device receives the Raman spectrum signal generated when the target bioparticle coupled with the Raman barcode bead is irradiated, and transfers the received Raman spectrum signal to the processor. The processor determines a type of the bioparticle(s) and calculates the number of bioparticle(s) by matching the Raman spectrum signal(s) to the Raman barcode database.

Abstract: Disclosed is a double-sided grating waveguide biosensor. The double-sided grating waveguide biosensor is used to sense the properties of a sample solution. The double-sided grating waveguide biosensor comprises a sequential stack of a plastic grating having a grating part, a waveguide layer having a double-sided grating structure, and a channel chip. Furthermore, the sample solution is guided into the channel chip; the light beam is coupled into the waveguide layer via the double-sided grating structure, propagates in the waveguide layer, and penetrates outward. The double-sided waveguide biosensor detects the properties of the sample solution via a variation of a light beam intensity of the outgoing light beam.

Abstract: A mercaptoalkylsilatrane derivative having protecting group and a method of manufacturing the same. The mercaptoalkylsilatrane derivative includes: mercaptoalkylsilatrane compound having a mercapto group; a protecting group bonding to sulfur of the mercapto group, wherein the protecting group is used to avoid the chemical reaction of the mercapto group with reactive chemical species, e.g., oxygen, ketone, and aldehyde, etc. Besides, the manufacturing method thereof includes the steps of: providing silane compound having the mercapto group; bonding the protecting group to the mercapto group of the silane compound; performing the chemical reaction of triethanolamine with the silane compound having the protecting group for manufacturing the mercaptoalkylsilatrane derivative having the protecting group.

Abstract: A multiplex fiber optic biosensor including an optical fiber, a plurality of noble metal nanoparticle layers, a plurality of light sources and a light source function generator is disclosed. The optical fiber includes a plurality of sensing regions which are unclad regions of the optical fiber so that the fiber core is exposed, wherein the noble metal nanoparticle layers are set in each sensing regions. The light sources emit light with different wavelengths, and the noble metal nanoparticle layers absorb the lights with different wavelengths, respectively. The light sources emit the lights in different timing sequences or different carrier frequencies, wherein when the lights propagate along the optical fiber in accordance with the different timing sequences or the different carrier frequencies, a detection unit detects particle plasmon resonance signals produced by interactions between the different noble metal nanoparticle layers and the corresponding analytes.

Abstract: The method for processing a sensor chip in accordance with the present invention has: 1) providing an acoustic wave operation system and a biochemical sensor chip, wherein the acoustic wave operation system has a piezoelectric transducer generating at least one cycle of longitudinal acoustic waves by a driving voltage and wherein a probe is immobilized on a surface of the biochemical sensor chip; 2) arranging the piezoelectric transducer at a distance from the biochemical sensor chip and filling therebetween with a medium for transmitting longitudinal acoustic waves; and 3) applying longitudinal acoustic waves to the biochemical sensor chip to remove an adsorbate bound to the probe.

Abstract: The present invention relates to a biochip with a piezoelectric element for ultrasonic standing wave generation. The biochip comprises an upper module, a lower module, and a chemical sensor. The piezoelectric element is integrated within the upper module of the biochip. The piezoelectric element can generate ultrasonic standing waves (USW) in the reaction chamber of the biochip by manipulating the operation frequency so the particles being detected can effectively move toward the QCM sensing surface. Hence, the biochip significantly increases the sensitivity and reduces the time required to reach equilibrium when undergoing USW excitation. The biochip of the present invention can be broadly applied to the bio-detection in medical and pharmaceutical fields.

Abstract: A mercaptoalkylsilatrane derivative having protecting group and a method of manufacturing the same. The mercaptoalkylsilatrane derivative includes: mercaptoalkylsilatrane compound having a mercapto group; a protecting group bonding to sulfur of the mercapto group, wherein the protecting group is used to avoid the chemical reaction of the mercapto group with reactive chemical species, e.g., oxygen, ketone, and aldehyde, etc. Besides, the manufacturing method thereof includes the steps of: providing silane compound having the mercapto group; bonding the protecting group to the mercapto group of the silane compound; performing the chemical reaction of triethanolamine with the silane compound having the protecting group for manufacturing the mercaptoalkylsilatrane derivative having the protecting group.

Abstract: A method for fixing metal onto a surface of the substrate. The present method includes steps of: providing a substrate and a mercaptoalkylsilatrane compound; dissolving the mercaptoalkylsilatrane compound in a solvent; performing a condensation reaction of the substrate with and the dissolved mercaptoalkylsilatrane compound to complete the surface modification of the substrate; and performing a covalent bonding process to metal with the mercaptoalkylsilatrane compound already modified onto the surface of the substrate to fix the metal onto the surface of the substrate.

Abstract: Disclosed is a double-sided grating waveguide biosensor. The double-sided grating waveguide biosensor is used to sense the properties of a sample solution. The double-sided grating waveguide biosensor comprises a sequential stack of a plastic grating having a grating part, a waveguide layer having a double-sided grating structure, and a channel chip. Furthermore, the sample solution is guided into the channel chip; the light beam is coupled into the waveguide layer via the double-sided grating structure, propagates in the waveguide layer, and penetrates outward. The double-sided waveguide biosensor detects the properties of the sample solution via a variation of a light beam intensity of the outgoing light beam.

Abstract: A method for obtaining the binding kinetic rate constants using fiber optic particle plasmon resonance (FOPPR) sensor, suitable for a test solution with two or more concentrations, which employs the following major steps: providing one FOPPR sensor instrument system, obtaining optical time-resolved signal intensities starting at the initial time to the steady state of the two or more regions, substituting the measured signal intensity values into the formula which is derived by using the pseudo-first order rate equation model. In addition, this method measures the temporal signal intensity evolution under static conditions as the samples are quickly loaded. As a result, unlike the conventional device where the sample is continuously infused, the method is able to measure the association and dissociation rate constants of which the upper bounds are not limited by the sample flow rate.

Abstract: A method for estimating binding kinetic rate constants by using a fiber optic particle plasmon resonance (FOPPR) sensor mainly employs the steps of: providing a FOPPR sensor instrument system, obtaining optical signal intensities at an initial time and steady state signal intensities of first and second regions in an intensity versus time graph separately, substituting the measured signal intensity values into a formula derived by using a pseudo-first order rate equation model. According to this method, no fluorophore labeling is required. In addition, this method measures a temporal signal intensity evolution under static conditions as the samples are quickly loaded. As a result, unlike the conventional device where the sample is continuously infused, the method is able to measure binding and decomposition rate constants whose upper limit is not limited by a sample flow rate.

Abstract: The method for processing a sensor chip in accordance with the present invention has: 1) providing an acoustic wave operation system and a biochemical sensor chip, wherein the acoustic wave operation system has a piezoelectric transducer generating at least one cycle of longitudinal acoustic waves by a driving voltage and wherein a probe is immobilized on a surface of the biochemical sensor chip; 2) arranging the piezoelectric transducer at a distance from the biochemical sensor chip and filling therebetween with a medium for transmitting longitudinal acoustic waves; and 3) applying longitudinal acoustic waves to the biochemical sensor chip to remove an adsorbate bound to the probe.

Abstract: A microfluidic device with microstructure includes a channel for accommodating an electrolytic solution therein and at least one microstructure formed in the channel. When an alternating-current signal is input to the microfluidic device so that a surface of the microstructure is polarized by a generated electric field, ions having polarity reverse to that of an electrolytic solution will migrate to the surface of the microstructure to form a field-induced electrical double layer to result in electro-osmotic flows at the corners at two sides of the microstructure, which causes formation of relatively fierce circular vortices in the solution. A sensing system and a sensing method using the microfluidic device with microstructure are also disclosed.

Abstract: The present invention relates to a biochip with a piezoelectric element for ultrasonic standing wave generation. The biochip comprises an upper module, a lower module, and a chemical sensor. The piezoelectric element is integrated within the upper module of the biochip. The piezoelectric element can generate ultrasonic standing waves (USW) in the reaction chamber of the biochip by manipulating the operation frequency so the particles being detected can effectively move toward the QCM sensing surface. Hence, the biochip significantly increases the sensitivity and reduces the time required to reach equilibrium when undergoing USW excitation. The biochip of the present invention can be broadly applied to the bio-detection in medical and pharmaceutical fields.

Abstract: A sensing system comprises a light source, an optical fiber, a plurality of noble metal nano-particles, a micro-fluidic module and a photo detector. The optical fiber couples an incident light. The plurality of noble metal nano-particles are disposed on a surface of the optical fiber to form a noble metal nano-particle submonolayer, the noble metal nano-particles are substantially separated from each adjacent noble metal nano-particles such that the conductivity of the noble metal nano-particle submonolayer is smaller than that of a metal film. The micro-fluidic module accommodates the optical fiber and a sample, and the sample is driven to contact with the noble metal nano-particles. The photo detector detects an emergent light from the optical fiber. When the incident light interacts with the noble metal nano-particles, a signal derived from localized surface plasmon resonance in form of attenuated light or elastic scattered light is outputted through the photo detector.

Abstract: A plasmon resonance sensing system includes a light source, a waveguide component and a photon detector. The light source provides an incident light. The waveguide component has a tubular internal wall and a noble metal nanoparticle layer disposed on the tubular internal wall and contacted with a desired testing matter. The waveguide component is made of a light transmitting material for guiding the incident light to have an interaction with the noble metal nanoparticle layer. The photon detector detects an emergent light exiting the waveguide component after the interaction of the noble metal nanoparticle layer with the desired testing matter. The system further includes a first optical fiber installed between the light source and the waveguide component for transmitting the incident light to the waveguide component, a lens and a second optical fiber. The lens collects and transmits the emergent light to the photon detector through the second optical fiber.