Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: Systems and methods for partially upgrading a produced heavy hydrocarbon resource at the production site (at the pad or a central processing facility) using indirect hydrogen addition, comprising a blender for blending the hydrocarbon with a hydrogen donator and a reactor for elevating the mixture to a temperature sufficient to hydrogenate the hydrocarbon to an API level required for pipeline specifications. The partially upgraded hydrocarbon can then be transported by pipeline to a refinery for further processing. The presence of catalysts and on-site hydrogen production equipment conventionally required for upgrading is eliminated. Further, the need for diluent which is conventionally used to enable pipeline ability of produced heavy hydrocarbon is reduced or eliminated, thus reducing the diluent freight cost and the required pipeline volume.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: A method for recognizing a voice and a device for recognizing a voice are provided. The method includes: collecting voice information input by a user; extracting characteristics from the voice information to obtain characteristic information; decoding the characteristic information according to an acoustic model and a language model obtained in advance to obtain recognized voice information, wherein the acoustic model is obtained by data compression in advance.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: A collimation system for a medical apparatus includes a collimation component having a laser emitting device, at least one photographing device, and a computing device. The laser emitting device is configured to the emit a laser beam to irradiate on a landmark on a body surface of a patient, the at least one photographing device is configured to take a photograph of a laser irradiation spot, and the computing device is configured to obtain a position of the laser irradiation spot by performing computation on images obtained by the at least one photographing device. The system further includes a moving component provided on the medical apparatus and configured to move to a target position for performing diagnosis or treatment. The medical apparatus automatically is configured to locate the moving component based on the position of the irradiation spot obtained from the computation.

Abstract: A system of medical imaging including a marking module, a classifying module, a region-of-interest determining module, a curve acquiring module and a delay calculating module. The marking module is used for marking one or more feature regions from pre-scanned images; the classifying module is used for classifying the feature regions by using a classification algorithm model; the region-of-interest determining module is used for selecting a feature region of the same type as a specific diagnostic tissue as a region of interest; the curve acquiring module is used for acquiring a curve of CT values of the region of interest in function of time, based on a relationship between the CT values and scanning time points; the delay calculating module is used for detecting a peak value of the curve and calculating a scan delay time based on a time point corresponding to the peak value.

Abstract: Disclosed is a voiceprint login method based on artificial intelligence. The method includes: S1: receiving a login request from a user and acquiring user information of the user; S2: generating a login string and replacing at least one character of the login string according to character replacement reference information corresponding to the user information; S3: providing the login string after replacement to the user and receiving speech information of the user reading the login string; and S4: performing a login authentication on the user according to speech information of the user reading the login string. With the method, by using the voiceprint authentication method combining the voiceprint and the character replacement reference information set by the user, the safety of the voiceprint password is increased, and some characters are hidden according the user's preferences, which satisfies the user's psychological demand of not wishing the password to be displayed explicitly.

Abstract: Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3? hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3? hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3? hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids.

Abstract: A method for recognizing a voice and a device for recognizing a voice are provided. The method includes: collecting voice information input by a user; extracting characteristics from the voice information to obtain characteristic information; decoding the characteristic information according to an acoustic model and a language model obtained in advance to obtain recognized voice information, wherein the acoustic model is obtained by data compression in advance.

Abstract: Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3? hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3? hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3? hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids.

Abstract: Single chain antibody polypeptides with engineered peptide bond crossovers in the light chain and/or heavy chain variable domains, compositions comprising the same, and methods of making and using the same are provided. The antibody polypeptides can be intercalated (crossover) single chain variable fragments (scFvs) or any antibody frameworks which comprise such scFvs, such as diabodies, bispecific antibodies or bssFvs. The single chain antibody polypeptides may or may not contain a linker. The single chain antibody polypeptides are useful in applications where standard (conventional) scFvs are useful, such as in the development of scFv libraries for screening, as therapeutic antibodies, or in in vitro and in vivo targeting applications.

Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.

Abstract: Systems and methods for partially upgrading a produced heavy hydrocarbon resource at the production site (at the pad or a central processing facility) using indirect hydrogen addition, comprising a blender for blending the hydrocarbon with a hydrogen donator and a reactor for elevating the mixture to a temperature sufficient to hydrogenate the hydrocarbon to an API level required for pipeline specifications. The partially upgraded hydrocarbon can then be transported by pipeline to a refinery for further processing. The presence of catalysts and on-site hydrogen production equipment conventionally required for upgrading is eliminated. Further, the need for diluent which is conventionally used to enable pipeline ability of produced heavy hydrocarbon is reduced or eliminated, thus reducing the diluent freight cost and the required pipeline volume.

Abstract: A corresponding grammar description file and a word category description file are defined based on a logical grammar by manifest language LGML according to a common sentence expression of a semantic meaning, in the grammar description file, the description for a common sentence is composed by operators, word categories, and functions, the word category description file is used to describe specific values for the word categories; the grammar description file and the word category description file are generated, with a reduction method according to a defined sequence, into a grammar tree for the grammar description file and word category trees for the word category description file; the word category trees are grafted at the positions of corresponding word categories on the grammar tree, forming a grammar tree for the semantic meaning, in this way, grammar compiling is accomplished.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Abstract: A method and system for producing steam for use in heavy hydrocarbon recovery operations. Water, containing impurities, produced from the well is separated from hydrocarbons and other materials, and subsequently directly passed to a produced water boiler with online pigging. The boiler is indirectly heated by means of a hot flue gas, such as a gas turbine exhaust, which produces electricity (for powering a central processing facility, or alternatively a burner to heat the boiler) and exhaust heat for heating the boiler.

Abstract: A method and system for producing steam for use in heavy hydrocarbon recovery operations. In an arrangement with or without a central processing facility and/or a plurality of well pads in communication with the central processing facility, each well pad is provided with equipment for separation of materials produced from its respective wells, and steam generation equipment for that well pad, thus allowing for simplified piping transport.

Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.