Abstract: A dust removal-sterilization-deodorization integrated intelligent lightweight device for livestock and poultry houses includes: a transparent housing, a photocatalyst slidably connected to an inner wall of the transparent housing, filter sleeves rotatably mounted on the inner wall of the transparent housing, a flow guide cavity defined in the transparent housing, a fan fixedly connected to a left sidewall of the flow guide cavity, and a flow baffle fixedly connected to an air inlet end of the flow guide cavity. The filter sleeve includes a low-resistance melt-blown fabric, a rutile manganese-zinc layer is laid on an outer side of the low-resistance melt-blown fabric, a skeleton fabric is attached to an outer wall of the rutile manganese-zinc layer, and an outer wall of the skeleton fabric is rotatably connected to the inner wall of the transparent housing. At least two filter sleeves are transversely arranged, and the filter sleeves are tubular.

Abstract: A dust removal-sterilization-deodorization integrated intelligent lightweight device for livestock and poultry houses includes: a transparent housing, a photocatalyst slidably connected to an inner wall of the transparent housing, filter sleeves rotatably mounted on the inner wall of the transparent housing, a flow guide cavity defined in the transparent housing, a fan fixedly connected to a left sidewall of the flow guide cavity, and a flow baffle fixedly connected to an air inlet end of the flow guide cavity. The filter sleeve includes a low-resistance melt-blown fabric, a rutile manganese-zinc layer is laid on an outer side of the low-resistance melt-blown fabric, a skeleton fabric is attached to an outer wall of the rutile manganese-zinc layer, and an outer wall of the skeleton fabric is rotatably connected to the inner wall of the transparent housing. At least two filter sleeves are transversely arranged, and the filter sleeves are tubular.

Abstract: Single-cell sequencing provides a new level of granularity in studying the heterogeneous nature of cancer cells. For some cancers, this heterogeneity is the result of copy number changes of genes within the cellular genomes. The ability to accurately determine such copy number changes is critical in tracing and understanding tumorigenesis. Current single-cell genome sequencing methodologies infer copy numbers based on statistical approaches followed by rounding decimal numbers to integer values. Such methodologies are sample dependent, have varying calling sensitivities which heavily depend on the sample's ploidy and are sensitive to noise in sequencing data. Described herein are novel methods for reconstructing the genome of a single cell.

Abstract: Provided are a locking mechanism and a washing machine. The washing machine includes a washing drum, the washing drum includes a container body having a via hole. The locking mechanism includes a locking shell connected to the container body and internally provided with an accommodating cavity communicated with the via hole; and a locking member inserted into the accommodating cavity.

Abstract: A method for quantitating a plurality of nucleic acid molecules is provided. The method includes contacting a plurality of detectably-labeled probes and a plurality of extension primers with the plurality of nucleic acid molecules, each detectably-labeled probe including a first labeled nucleic acid domain having a first label; producing an extension product of each of the plurality of target nucleic acid fragments by extending a respective one of the plurality of extension primers with a polymerase; hydrolyzing each of the plurality of detectably-labeled probes hybridized to the respective one of the target nucleic acid fragments during extending the respective one of the plurality of extension primers with the polymerase; detecting a first signal produced as a result of hydrolyzing the plurality of detectably-labeled probes; and calculating a number of the plurality of target nucleic acid fragments based on signals detected upon a single cycle of extension reactions.

Abstract: This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.

Abstract: This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.

Abstract: The preset disclosure provides a washing machine inner cylinder assembly and a drum-type washing machine. The washing machine inner cylinder assembly includes an inner cylinder, a support, a rotation shaft, a cover, and a sealing strip. The support includes a hub portion and a leg portion extending outwards from periphery of hub portion. End of rotation shaft is fixedly embedded in a shaft hole of hub portion. The inner cylinder includes a cylinder body and a cylinder bottom at an end of cylinder body. A groove is defined in outer surface of cylinder bottom for receiving and clamping the leg portion. The cover covers and connects to the support. The sealing strip is arranged between the cover and cylinder bottom. The sealing strip, the cover, and the groove cooperatively define a closed space for receiving the support.

Abstract: Provided are a locking mechanism and a washing machine. The washing machine includes a washing drum, the washing drum includes a container body having a via hole. The locking mechanism includes a locking shell connected to the container body and internally provided with an accommodating cavity communicated with the via hole; and a locking member inserted into the accommodating cavity.

Abstract: Disclosed is a washing machine. The washing machine comprises an impeller, a decelerating clutch and an impeller connection assembly, wherein the decelerating clutch has a rotary shaft; and the impeller connection assembly is clamped and clamped and fixedly connected to the rotary shaft, so that the impeller is clamped and fixedly connected to the rotary shaft in a detachable way, thereby reducing the difficulties of the assembly and disassembly of the impeller, and prolonging the service life of the washing machine.

Abstract: Provided are a washing machine and a washing drum thereof. The washing drum comprises a large container and a small container. The small container is detachably disposed in the large container, and an opening of the small container and an opening of the large container are positioned opposite to each other. In a washing process, clothes needing special care can be placed in the small container so that water in the large container does not enter the small container, thereby realizing separating washing of the clothes needing special care. If there are no clothes needing special care, the small container can be detached from the large container to perform a normal washing operation.

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5? phosphate group by removing an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract: In certain embodiments, the present invention provides a way of “digitally” marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.

Abstract: The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.

Abstract: This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.

Abstract: This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.

Abstract: The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5? phosphate group by removing an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5? phosphate group by removing an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract: The preset disclosure provides a washing machine inner cylinder assembly and a drum-type washing machine. The washing machine inner cylinder assembly includes an inner cylinder, a support, a rotation shaft, a cover, and a sealing strip. The support includes a hub portion and a leg portion extending outwards from periphery of hub portion. End of rotation shaft is fixedly embedded in a shaft hole of hub portion. The inner cylinder includes a cylinder body and a cylinder bottom at an end of cylinder body. A groove is defined in outer surface of cylinder bottom for receiving and clamping the leg portion. The cover covers and connects to the support. The sealing strip is arranged between the cover and cylinder bottom. The sealing strip, the cover, and the groove cooperatively define a closed space for receiving the support.