Abstract: Provided are a washing machine and a washing drum thereof. The washing drum comprises a large container and a small container. The small container is detachably disposed in the large container, and an opening of the small container and an opening of the large container are positioned opposite to each other. In a washing process, clothes needing special care can be placed in the small container so that water in the large container does not enter the small container, thereby realizing separating washing of the clothes needing special care. If there are no clothes needing special care, the small container can be detached from the large container to perform a normal washing operation.

Abstract: Disclosed is a washing machine. The washing machine comprises an impeller, a decelerating clutch and an impeller connection assembly, wherein the decelerating clutch has a rotary shaft; and the impeller connection assembly is clamped and clamped and fixedly connected to the rotary shaft, so that the impeller is clamped and fixedly connected to the rotary shaft in a detachable way, thereby reducing the difficulties of the assembly and disassembly of the impeller, and prolonging the service life of the washing machine.

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5? phosphate group by removing an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5? phosphate group by removing an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract: The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.

Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.

Abstract: The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or ?-monothiopyrophosphate), and related compounds.

Abstract: A lens system for use with photoacoustic microscopy apparatus has a collimated single mode optical fiber to which a toroidal ultrasound transducer is operatively attached. The transducer is located inside a tank. A lens housing is located inside the tank adjacent the transducer and has flexible optically transmissive entrance and exit ports made of polydimethysiloxane. The lens housing is filled with cinnamaldehyde. The cinnamaldehyde can be introduced into the lens housing and withdrawn from it so as to flex its entrance and exit ports, and the tank is filled with a mixture of glycerol and water.

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or ?-monothiopyrophosphate), and related compounds.

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5? phosphate group by removing an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5? phosphate group by removing an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.

Abstract: A lens system for use with photoacoustic microscopy apparatus has a collimated single mode optical fiber to which a toroidal ultrasound transducer is operatively attached. The transducer is located inside a tank. A lens housing is located inside the tank adjacent the transducer and has flexible optically transmissive entrance and exit ports made of polydimethysiloxane. The lens housing is filled with cinnamaldehyde. The cinnamaldehyde can be introduced into the lens housing and withdrawn from it so as to flex its entrance and exit ports, and the tank is filled with a mixture of glycerol and water.

Abstract: In certain embodiments, the present invention provides a way of “digitally” marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons into genomic DNA before further analysis. According to this method, each allele becomes marked with a unique pattern of transposon barcodes. Because each unique pattern of transposon barcodes identifies a particular allele, the method facilitates determinations of ploidy and copy number variation, improves the ability to discriminate among homozygotes, heterozygotes, and patterns arising from sequencing errors, and allows loci separated by uninformative stretches of DNA to be identified as linked loci, thereby facilitating haplotype determinations. Also provided is a novel artificial transposon end that includes a barcode sequence in two or more positions that are not essential for transposition.

Abstract: The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.

Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or ?-monothiopyrophosphate), and related compounds.

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5? phosphate group by removing an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5? phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract: The present invention relates to the generation and construction of libraries for genome-wide antisense oligonucleotide and siRNA.