Patents by Inventor Lei Xi

Lei Xi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods of Using Improved Polymerases
    Publication number: 20140220577
    Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.
    Type: Application
    Filed: June 21, 2013
    Publication date: August 7, 2014
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: YAN WANG, PETER VANDER HORN, LEI XI
  • Method for diagnosing acute ischemic events in human subjects
    Patent number: 8609360
    Abstract: Chemiluminescent of metabolic by-products of inosine and hypoxanthine is used to diagnose acute ischemic events in human subjects.
    Type: Grant
    Filed: November 29, 2012
    Date of Patent: December 17, 2013
    Assignee: Virginia Commonwealth University
    Inventors: Don Farthing, Lei Xi, H. Thomas Karnes, Domenic Sica, Todd Gehr, Lynne Gehr, Martin Unverdorben
  • METHOD OF MAKING A PAIRED TAG LIBRARY FOR NUCLEIC ACID SEQUENCING
    Publication number: 20130252851
    Abstract: The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.
    Type: Application
    Filed: May 14, 2013
    Publication date: September 26, 2013
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Bin LI, Lei XI, Swati RANADE, Yangzhou WANG
  • Method of making a paired tag library for nucleic acid sequencing
    Patent number: 8530197
    Abstract: The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.
    Type: Grant
    Filed: January 8, 2009
    Date of Patent: September 10, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Bin Li, Lei Xi, Swati S. Ranade, Yangzhou Wang
  • Methods of using improved polymerases
    Patent number: 8476045
    Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.
    Type: Grant
    Filed: July 27, 2012
    Date of Patent: July 2, 2013
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yan Wang, Peter Vander Horn, Lei Xi
  • Quantitative amplification with a labeled probe and 3? to 5? exonuclease activity
    Patent number: 8426132
    Abstract: This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3? to 5? exonuclease activity that cleaves the 3? oligonucleotide of the probe.
    Type: Grant
    Filed: November 3, 2008
    Date of Patent: April 23, 2013
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Bin Li, Lei Xi, Yan Wang, Peter B. Vander Horn
  • Method for diagnosing acute cardiac ischemia
    Patent number: 8343731
    Abstract: Chemiluminescent detection of metabolic by-products of inosine and hypoxanthine is used to diagnose ischemic events such as early acute cardiac ischemia.
    Type: Grant
    Filed: August 1, 2008
    Date of Patent: January 1, 2013
    Assignee: Virginia Commonwealth University
    Inventors: Don Farthing, Lei Xi, H. Thomas Karnes, Domenic Sica, Todd Gehr, Lynne Gehr, Martin Unverdorben
  • METHODS OF USING IMPROVED POLYMERASES
    Publication number: 20120295270
    Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.
    Type: Application
    Filed: July 27, 2012
    Publication date: November 22, 2012
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Yan Wang, Peter Vander Horn, Lei Xi
  • Methods of using improved polymerases
    Patent number: 8232078
    Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.
    Type: Grant
    Filed: November 27, 2002
    Date of Patent: July 31, 2012
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yan Wang, Peter Vander Horn, Lei Xi
  • Polymerase Assay with a FRET Substrate
    Publication number: 20120070838
    Abstract: This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.
    Type: Application
    Filed: August 19, 2011
    Publication date: March 22, 2012
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Lei Xi, Keith P. Bjornson, Stephen P. Hendricks
  • METHOD FOR DIAGNOSING ACUTE CARDIAC ISCHEMIA
    Publication number: 20110171671
    Abstract: Chemiluminescent detection of metabolic by-products of inosine and hypoxanthine is used to diagnose ischemic events such as early acute cardiac ischemia.
    Type: Application
    Filed: August 1, 2008
    Publication date: July 14, 2011
    Inventors: Don Farthing, Lei Xi, Thomas H. Karnes, Domenic Sica, Todd Gehr
  • QUANTITATIVE AMPLIFICATION WITH A LABELED PROBE AND 3' TO 5' EXONUCLEASE ACTIVITY
    Publication number: 20100159447
    Abstract: This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3? to 5? exonuclease activity that cleaves the 3? oligonucleotide of the probe.
    Type: Application
    Filed: November 3, 2008
    Publication date: June 24, 2010
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Bin Li, Lei Xi, Yan Wang, Peter B. Vander Horn
  • Nucleic acid modifying enzymes
    Patent number: 7670808
    Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
    Type: Grant
    Filed: April 9, 2004
    Date of Patent: March 2, 2010
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yan Wang, Lei Xi, Dennis E. Prosen
  • Methods and Compositions for PCR
    Publication number: 20090263869
    Abstract: A modified thermostable Pol B DNA polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable Pol B DNA polymerase and a modifier reagent of Formula I wherein the reaction results in a thermally reversible inactivation of the thermostable Pol B DNA polymerase activity and the 3?-5? exonuclease activity, which polymerase is suitable for hot-start PCR. Also disclosed are the method for the modification, a polynucleic acid amplification method and PCR reaction mixture and kit comprising the modified thermostable Pol B DNA polymerase.
    Type: Application
    Filed: August 27, 2008
    Publication date: October 22, 2009
    Inventors: Lei Xi, Dennis E. Prosen, Alexander A. Novikov
  • METHOD OF MAKING A PAIRED TAG LIBRARY FOR NUCLEIC ACID SEQUENCING
    Publication number: 20090181861
    Abstract: The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.
    Type: Application
    Filed: January 8, 2009
    Publication date: July 16, 2009
    Inventors: Bin Li, Lei Xi, Swati S. Ranade, Yangzhou Wang
  • Nucleic acid modifying enzymes
    Patent number: 7541170
    Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
    Type: Grant
    Filed: May 30, 2001
    Date of Patent: June 2, 2009
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yan Wang, Lei Xi, Dennis E. Prosen
  • Quantitative amplification with a labeled probe and 3? to 5? exonuclease activity
    Patent number: 7445898
    Abstract: This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3? to 5? exonuclease activity that cleaves the 3? oligonucleotide of the probe.
    Type: Grant
    Filed: March 31, 2005
    Date of Patent: November 4, 2008
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Bin Li, Lei Xi, Yan Wang, Peter B. Vander Horn
  • Polymorphism and haplotype scoring by differential amplification of polymorphisms
    Publication number: 20060051749
    Abstract: The current invention provides a method of performing DNA polymorphism assays. The assay combines allele-specific PCR with technology used for quantitative PCR. The method can be used to score the presence of absence of particular polymorphisms in a DNA sample. In a further aspect of the invention, the method is used to score the presence or absence of particular haplotypes in a DNA sample.
    Type: Application
    Filed: November 27, 2002
    Publication date: March 9, 2006
    Applicant: MJ BIOWORKS INCORPORATED
    Inventors: Yan Wang, Lei Xi, Michael Finney, Fan Chen
  • Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
    Publication number: 20060024695
    Abstract: This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3? to 5? exonuclease activity that cleaves the 3? oligonucleotide of the probe.
    Type: Application
    Filed: March 31, 2005
    Publication date: February 2, 2006
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: Bin Li, Lei Xi, Yan Wang, Peter Vander Horn
  • Nucleic acid modifying enzymes
    Publication number: 20040191825
    Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.
    Type: Application
    Filed: April 9, 2004
    Publication date: September 30, 2004
    Applicant: MJ Bioworks, Inc.
    Inventors: Yan Wang, Lei Xi, Dennis E. Prosen