Abstract: Disclosed are methods of in situ genomic modification of cells (e.g., stem cells, tissue stem cells, muscle stem cells, Sca-1+ mesenchymal progenitor cells in skeletal muscle, CD140a+ dermal mesenchymal cells) using sequence-targeting nucleases delivered via a virus (e.g., an AAV).

Abstract: The present disclosure provides microRNA-based therapies using a hydrogel delivery system that provides regenerative approach to myocardial infarction by targeting cardiomyocytes. The hydrogel provides for local and sustained cardiac delivery of microRNAs, such miR-302 mimics that can be used to promote cardiomyocyte proliferation. Also provided are compositions suitable for local and sustained release and methods for intramyocardial gel delivery of a miRNA oligonucleotide.

Abstract: The present disclosure provides microRNA-based therapies using a hydrogel delivery system that provides regenerative approach to myocardial infarction by targeting cardiomyocytes. The hydrogel provides for local and sustained cardiac delivery of microRNAs, such miR-302 mimics that can be used to promote cardiomyocyte proliferation. Also provided are compositions suitable for local and sustained release and methods for intramyocardial gel delivery of a miRNA oligonucleotide.

Abstract: Disclosed are methods of in situ genomic modification of cells (e.g., stem cells, tissue stem cells, muscle stem cells, Sca-V mesenchymal progenitor cells in skeletal muscle, CD140a+ dermal mesenchymal cells) using sequence-targeting nucleases delivered via a virus (e.g., an AAV).

Abstract: Known lipid molecules of a matrix are grouped into lipid classes and the lipid classes are further grouped into a pass-through group and a mobility separation group based on isobaric interferences. A separation system separates known lipid molecules from a matrix sample and an ion source ionizes the matrix sample. Two injections are performed. For the first injection a DMS device is put into passive mode, and for the second injection the DMS device is used to resolve isobaric interferences. A tandem mass spectrometer performs MRM scans of the pass-through group for the first injection and MRM scans of the mobility separation group for the second injection. A processor quantitates each lipid molecule in the matrix sample by comparing the MRM intensity values obtained for the first and second injections to MRM intensity and concentration values for known standards of the known lipid molecules of the matrix.

Abstract: Disclosed are methods of modifying the genome of HSPCs in vivo by introducing an AAV into a subject transducing a sequence targeting nuclease. In some aspects, the method can be utilized to ascertain causal links between CHIP mutations and age-associated disease. In other aspects, the method can be utilized to treat Sickle cell disease (SCD) and ?-thalassemia.

Abstract: Systems and methods for collecting weather information for selected airspace regions are provided. In one embodiment, a method for collecting weather information for selected airspace regions comprises: receiving aircraft position information for a plurality of aircraft; forming an aircraft weather group based on flight path attributes derived from the aircraft position information; selecting at least a first representative aircraft from the weather group; and receiving at a weather information ground station, weather data from one or more representative aircraft of the aircraft weather group, wherein only the one or more representative aircraft transmit weather information to the weather information ground station from the aircraft weather group.

Abstract: Systems and methods for collecting weather information for selected airspace regions are provided. In one embodiment, a method for collecting weather information for selected airspace regions comprises: receiving aircraft position information for a plurality of aircraft; forming an aircraft weather group based on flight path attributes derived from the aircraft position information; selecting at least a first representative aircraft from the weather group; and receiving at a weather information ground station, weather data from one or more representative aircraft of the aircraft weather group, wherein only the one or more representative aircraft transmit weather information to the weather information ground station from the aircraft weather group.

Abstract: The present disclosure provides microRNA-based therapies using a hydrogel delivery system that provides regenerative approach to myocardial infarction by targeting cardiomyocytes. The hydrogel provides for local and sustained cardiac delivery of microRNAs, such miR-302 mimics that can be used to promote cardiomyocyte proliferation. Also provided are compositions suitable for local and sustained release and methods for intramyocardial gel delivery of a miRNA oligonucleotide.

Abstract: A self-contained breathing apparatus. The self-contained breathing apparatus comprises a back plate comprising a shoulder strap load portion and a waist belt load portion, wherein the waist belt load portion rotates about an extended axis defined by the junction of the waist belt load portion with the shoulder strap load portion, a shoulder strap coupled to the shoulder strap load portion of the back plate, a waist belt coupled to the waist belt load portion of the back plate, and a bottle of compressed breathable air secured to the shoulder strap load portion of the back plate.

Abstract: Systems and methods for collecting weather information for selected airspace regions are provided. In one embodiment, a method for collecting weather information for selected airspace regions comprises: receiving aircraft position information for a plurality of aircraft; forming an aircraft weather group based on flight path attributes derived from the aircraft position information; selecting at least a first representative aircraft from the weather group; and receiving at a weather information ground station, weather data from one or more representative aircraft of the aircraft weather group, wherein only the one or more representative aircraft transmit weather information to the weather information ground station from the aircraft weather group.

Abstract: Systems and methods for collecting weather information for selected airspace regions are provided. In one embodiment, a method for collecting weather information for selected airspace regions comprises: receiving aircraft position information for a plurality of aircraft; forming an aircraft weather group based on flight path attributes derived from the aircraft position information; selecting at least a first representative aircraft from the weather group; and receiving at a weather information ground station, weather data from one or more representative aircraft of the aircraft weather group, wherein only the one or more representative aircraft transmit weather information to the weather information ground station from the aircraft weather group.

Abstract: Systems and methods for regulating weather information collection are provided. In one embodiment, a method for managing the collection of weather information comprises: determining whether or not a weather data processing system is in a state open to receiving weather information; communicating to a first aircraft when the weather data processing system is in a state open to receiving weather information; and upon receiving weather information at the weather data processing system from the first aircraft, transmitting a signal indicating that the weather data processing system is not in a state open to receiving weather information.

Abstract: Known lipid molecules of a matrix are grouped into lipid classes and the lipid classes are further grouped into a pass-through group and a mobility separation group based on isobaric interferences. A separation system separates known lipid molecules from a matrix sample and an ion source ionizes the matrix sample. Two injections are performed. For the first injection a DMS device is put into passive mode, and for the second injection the DMS device is used to resolve isobaric interferences. A tandem mass spectrometer performs MRM scans of the pass-through group for the first injection and MRM scans of the mobility separation group for the second injection. A processor quantitates each lipid molecule in the matrix sample by comparing the MRM intensity values obtained for the first and second injections to MRM intensity and concentration values for known standards of the known lipid molecules of the matrix.

Abstract: Systems and methods for regulating weather information collection are provided. In one embodiment, a method for managing the collection of weather information comprises: determining whether or not a weather data processing system is in a state open to receiving weather information; communicating to a first aircraft when the weather data processing system is in a state open to receiving weather information; and upon receiving weather information at the weather data processing system from the first aircraft, transmitting a signal indicating that the weather data processing system is not in a state open to receiving weather information.

Abstract: Systems and methods for collecting weather information for selected airspace regions are provided. In one embodiment, a method for collecting weather information for selected airspace regions comprises: receiving aircraft position information for a plurality of aircraft; forming an aircraft weather group based on flight path attributes derived from the aircraft position information; selecting at least a first representative aircraft from the weather group; and receiving at a weather information ground station, weather data from one or more representative aircraft of the aircraft weather group, wherein only the one or more representative aircraft transmit weather information to the weather information ground station from the aircraft weather group.

Abstract: Meeting participants of a videoconference can be enabled to alert the other meeting participants that they are going to be away from or inattentive during a portion of the videoconference. For example, each meeting participant can be presented with a user interface element, such as a button, that the meeting participant can select to alert the other meeting participants that the meeting participant will be away or inattentive during a portion of the videoconference. The meeting participants can also be enabled to alert an away meeting participant to rejoin the videoconference. For example, meeting participants can be presented with a user interface element, such as a button, that can be selected to send an alert to an away meeting participant to rejoin the teleconference. A meeting participant can also select to be alerted when a specified alert trigger occurs, such as when a specified word or phrase is used.

Abstract: A self-contained breathing apparatus. The self-contained breathing apparatus comprises a back plate comprising a shoulder strap load portion and a waist belt load portion, wherein the waist belt load portion rotates about an extended axis defined by the junction of the waist belt load portion with the shoulder strap load portion, a shoulder strap coupled to the shoulder strap load portion of the back plate, a waist belt coupled to the waist belt load portion of the back plate, and a bottle of compressed breathable air secured to the shoulder strap load portion of the back plate.

Abstract: A flash memory cell including a first conductive type substrate, a second conductive type well, a patterned film layer, a second conductive type doped region, a tunneling dielectric layer, a plurality of floating gates, an inter-gate dielectric layer and a plurality of control gates is provided. The floating gates are formed on the first conductive type substrate outside the patterned film layer. The floating gates have a thickness greater than the patterned film layer. Thus, the overlapping area between the floating gates and the control gates and hence the coupling ratio of the flash memory cell is increased.