Abstract: This invention relates to a lubricating oil composition comprising or resulting from the admixing of: a) 50 to 99 mass % of one or more base oils; b) 0.1 to 15 mass % of one or more functionalized olefin copolymer having an Mn of from 10,000 to 35,000 g/mol; c) 0.1 to 20 mass % of one or more detergents wherein the detergent comprises at least one calcium detergent having a TBN of more than 100 mg KOH/g; at least one calcium detergent having a TBN of 100 mg KOH/g or less; and at least one magnesium detergent, where the difference in the TBN's for the Ca detergents is at least 100 mg KOH/g; d) 0.01 to 40 mass % of one or more dispersants wherein the dispersants comprise at least one borated dispersant and at least one non borated dispersant; e) 0.01 to 20 of mass % sulfurized fatty acid ester; f) 0.01 to 5 mass % of one or more amine or phenol based antioxidants; and g) 0.001 to 15 mass % of molybdenum containing compound, where the lubricating oil composition has: 1) a sulfated ash content of 0.

Abstract: The disclosure provides a system and method for a programmable medical wire that can be preprogrammed, and controlled and reshaped upon command. The system can include a power supply, a controller, and a multilayered wire assembly. The wire assembly includes a core conductor, actuator conductors coupled to the core conductor, selective conductors formed adjacent the core conductor and the actuator conductors, and a protective biocompatible shield around the layers. The selective conductors can be energized to activate the actuator conductors and cause the actuator conductors to bend or twist in a preprogrammed manner. By selectively controlling the direction of movement of the actuator conductors, the wire assembly can be remotely guided through body passageways to the target. Auxiliary equipment such as sensors, micro cameras, detectors, cutters, and other equipment can also be coupled to the wire assembly, and controlled and communicated with through one or more of the selective conductors.

Abstract: Provided herein are reagents, compositions, and methods for sequencing nucleic acids. Among the reagents disclosed are nucleotides labeled with cleavable linkers. Certain linkers are configured to undergo immolation reactions following cleavage, which can generate scars with enhanced properties for nucleic acid sequencing and synthesis. The present disclosure also provides reagents, compositions, and methods for capping scars generated through linker cleavage, which can alter scar properties, and in some cases can increase the rate and accuracy of nucleotide incorporations during polymerization.

Abstract: The present disclosure is generally directed to an apparatus and method for minting NFTs from user specific events. Minting NFTs from user specific events may include receiving user data and classifying the user data to a plurality of user specific events. Further, minting may include selecting a user specific event of the plurality of user specific events. Moreover, minting may include recording the selected user specific event of the plurality of user specific events to an immutable sequential listing, where recording the selected user specific event comprises minting the selected user specific event into an NFT for storage on the immutable sequential listing.

Abstract: A treatment process for remediating contaminants in a mixture of contaminated fluids, including at least one liquid fluid and at least one gaseous fluid, includes the steps of: preparing a liquid treatment composition containing at least 80 volume % of an aqueous solution containing at least one hydroxide compound at a collective concentration of 35-55 weight percent, and at least one of fulvic acid and humic acid at a collective concentration of 0.1-5 wt % of the treatment composition; adding a dosage of the treatment composition to a mixture of contaminated fluids including a liquid portion and a gaseous portion; and allowing the treatment composition to react with the mixture of contaminated fluids for at least 10 minutes, wherein a pH of the treatment composition is at least 13.0 and the aqueous solution contains at least one of NaOH and KOH.

Abstract: Energy transfer dye pairs including a donor dye covalently attached to an acceptor dye through a linker, uses of the energy transfer dye pairs, for example, in conjugates of an energy transfer dye pair covalently attached to a quencher and an analyte (e.g., an oligonucleotide), for biological applications including, for example, amplification assays such as quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR).

Abstract: An apparatus and method for temporarily sharing a non-fungible token (NFT), the apparatus includes at least a processor, a memory communicatively connected to the at least processor, wherein the memory containing instructions configuring the at least processor to receive a data collection, wherein the data collection comprises a plurality of data objects associated with at least one NFT, verify the at least one NFT as a function of the data collection, identify an in-force time element as a function of the at least one NFT, determine at least one designated utility of the at least one NFT, and generate a self-executing action protocol for interests to the at least one NFT as a function of the in-force time element and the at least one designated utility of the at least one NFT.

Abstract: An apparatus and method for generating NFTs from user-specific products and data, the apparatus including at least a processor, a memory communicatively connected to the at least processor, wherein the memory containing instructions configuring the at least processor to receive a data collection from a user, wherein the data collection comprising a plurality of user-specific data objects, assess a plurality of user categories as a function of the data collection, identify a value function as a function of the plurality of user-specific data objects and the plurality of user categories, optimize the value function to generate a ranked plurality of user-specific data objects, generate a recommendation for the NFT as a function of the ranked plurality of user-specific data objects, and generate the NFT as a function of the recommendation.

Abstract: An apparatus and method for executing a transaction protocol for rights to a non-fungible token (NFT), the apparatus includes at least a processor, a memory communicatively connected to the at least processor, wherein the memory containing instructions configuring the at least processor to receive a data collection, wherein the data collection comprises a plurality of data objects associated with at least one NFT, verify the at least one NFT as a function of the data collection, determine at least one designated utility of the at least one NFT, and generate a self-executing transaction protocol for rights to the at least one NFT as a function of the at least one designated utility of the at least one NFT.

Abstract: The present disclosure provides methods and systems for processing a nucleotide mixture. A nucleotide mixture can be purified. A nucleotide mixture can be processed for use in nucleic acid synthesis. A nucleotide mixture can be processed for use in nucleic acid sequencing.

Abstract: The present disclosure provides methods and systems for determining a nucleic acid sequence of a target nucleic acid molecule. A method for sequencing a target nucleic acid molecule comprises subjecting a plurality of nucleic acid molecules exhibiting sequence homology to the target nucleic acid molecule to at most 4000 cycles a nucleic acid extension reaction while measuring detectable signals from the plurality of nucleic acid molecules. The detectable signals may correspond to individual nucleotides or nucleotide analogs incorporated into the plurality of nucleic acid molecules during the nucleic acid extension reaction. The detectable signals may be used to generate a sequence of the target nucleic acid molecule at a length of at least about 500 bases and an accuracy of at least about 97%.

Abstract: A system (1000) comprising first and second excitation sources (101a, 101b) with respective excitation wavelengths for exciting first second and third dyes in a sample (110) and further comprising a detector (115), first and second emission spectral elements (121a, 121b) for transmitting respective first and second emission wavelengths as well as a processor (130) for automatically operating the elements of the system. The first dye comprises a first absorption spectrum comprising a first maximum absorption wavelength and the second dye comprises a second absorption spectrum comprising a second maximum absorption wavelength that is equal to or substantially equal to the first maximum absorption wavelength. The second dye comprises a second emission spectrum comprising a second maximum emission wavelength and the third dye comprises a third emission spectrum comprising a third maximum emission wavelength that is equal to or substantially equal to the second maximum emission wavelength.

Abstract: An apparatus and method for vetting a user using a computing device, wherein the apparatus includes at least a processor, a memory communicatively connected to the at least a processor, wherein the memory containing instructions configuring the at least a processor to receive a data collection from a user, wherein the data collection includes a plurality of data objects, construct a decision tree as a function of the data collection, wherein the decision tree includes a plurality of nodes, generate a trust datum as a function of the decision tree, and verify the user as a function of the trust datum, and a display communicatively connected to the at least a processor configured to present a visual interface.

Abstract: The present disclosure provides methods and systems for processing a nucleotide mixture. A nucleotide mixture can be purified. A nucleotide mixture can be processed for use in nucleic acid synthesis. A nucleotide mixture can be processed for use in nucleic acid sequencing.

Abstract: The present disclosure provides methods and systems for nucleic acid sequencing. Such systems and methods may achieve context-independent incorporation, have reduced context-dependence or have context-dependence that is amenable to calibration and modeling. Such systems and methods may also reduce misincorporation.

Abstract: A method for forming discrete volumes of aqueous fluid may comprise flowing aqueous fluid into a first conduit from a supply of aqueous fluid and flowing into the first conduit a spacing liquid supplied from a second conduit, the spacing liquid being immiscible with the aqueous fluid. The flowing of the aqueous fluid and the spacing liquid into the first conduit forms discrete volumes of the aqueous fluid, with consecutive discrete volumes of the aqueous fluid separated by the spacing liquid. The method may further comprise transferring the discrete volumes of the aqueous fluid and spacing liquid from the first conduit to a third conduit for processing.

Abstract: The disclosure provides methods for sequencing nucleic acids using, including with nucleotide analogs and subsequently appended labels.

Abstract: The present disclosure provides methods for nucleic acid sequence identification. The methods may comprise bringing a plurality of nucleic acid molecules in contact with a reaction mixture including a concentration of nucleotides that results in fractional labeling of the nucleic acid molecules. The methods may comprise starting a next reversibly-terminated, sequencing cycle prior to completion of unblocking of reversible terminators in a previous sequencing cycle.

Abstract: The present disclosure provides methods and systems for nucleic acid sequencing. Such systems and methods may achieve context-independent incorporation, have reduced context-dependence or have context-dependence that is amenable to calibration and modeling. Such systems and methods may also reduce misincorporation.

Abstract: The disclosure provides methods for sequencing nucleic acids using, including with nucleotide analogs and subsequently appended labels.