Patents by Inventor Louis-Philippe Vezina
Louis-Philippe Vezina has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10272148Abstract: A method for synthesizing chimeric influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of chimeric influenza HA in a plant or a portion of a plant. The invention is also directed towards a VLP comprising chimeric influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding chimeric influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: GrantFiled: June 25, 2010Date of Patent: April 30, 2019Assignee: MEDICAGO INC.Inventors: Manon Couture, Michele Dargis, Pierre-Olivier Lavoie, Louis-Philippe Vezina, Marc-Andre D'Aoust
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Publication number: 20190112341Abstract: A method of producing a picornavirus-like particle (PVLP) in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, portion of the plant, or a plant cell. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding a polyprotein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more protease. The plant, portion of the plant, or plant cell is incubated under conditions that permit the expression of the nucleic acids, thereby producing the PVLP. A PVLP comprising the polyprotein is also provided.Type: ApplicationFiled: December 20, 2018Publication date: April 18, 2019Inventors: MARC-ANDRE D'AOUST, PIERRE-OLIVIER LAVOIE, MANON COUTURE, LUCIE POULIN, LOUIS-PHILIPPE VEZINA
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Patent number: 10202423Abstract: A method of producing a picornavirus-like particle (PVLP) in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, portion of the plant, or a plant cell. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding a polyprotein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more protease. The plant, portion of the plant, or plant cell is incubated under conditions that permit the expression of the nucleic acids, thereby producing the PVLP. A PVLP comprising the polyprotein is also provided.Type: GrantFiled: August 29, 2013Date of Patent: February 12, 2019Assignee: Medicago Inc. of Quebec, CanadaInventors: Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Manon Couture, Lucie Poulin, Louis-Philippe Vezina
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Patent number: 10190132Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plant lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: GrantFiled: September 2, 2016Date of Patent: January 29, 2019Assignee: MEDICAGO INC.Inventors: Marc-Andre D'Aoust, Manon Couture, Frederic Ors, Sonia Trepanier, Pierre-Olivier Lavoie, Michele Dargis, Louis-Philippe Vezina, Nathalie Landry
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Patent number: 10058039Abstract: It is provided a plant infiltration device for infiltrating plants with an inoculum and method of using same, the device comprising a frame having one or more tray racks adapted for receiving at least one tray of plants to be infiltrated; an automated manipulation mechanism mounted to the frame and operable to displace the plant trays within the device; a plurality of inoculum reservoirs mounted to the frame and containing one or more inoculum fluids; an infiltration tank mounted to the frame and within which the plant trays are received when disposed in the infiltration position; and a vacuum generating device in communication with the infiltration tank to apply a negative pressure within the infiltration tank, thereby opening pores of the plant leaves immersed in the inoculum within the infiltration tank and causing the inoculum fluid to infiltrate into the plant leaves.Type: GrantFiled: February 15, 2013Date of Patent: August 28, 2018Assignee: MEDICAGO INC.Inventors: Marc-Andre D'Aoust, Nicole Bechtold, Luc Laurin, Louis-Philippe Vezina, Normand Dubuc
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Patent number: 9815873Abstract: Methods of recovering plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, loosening the cell wall to produce a plant or plant matter having a loosened cell wall, thus allowing the release of apoplastic content through the cell wall to produce an apoplastic content fraction from the plant or plant matter, and recovering the apoplastic content fraction. The apoplastic content fraction comprises plant-derived proteins or suprastructure proteins.Type: GrantFiled: March 23, 2012Date of Patent: November 14, 2017Assignee: MEDICAGO INC.Inventors: Manon Couture, Dany Paquet, Louis-Philippe Vezina
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Publication number: 20170247714Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.Type: ApplicationFiled: March 30, 2017Publication date: August 31, 2017Applicants: Medicago, Inc., Centre National de la Recherche Scientifique, Universite De RouenInventors: Thomas PACCALET, Muriel BARDOR, Christophe RIHOUEY, Véronique GOMORD, Loïc FAYE, Patrice LEROUGE, Stéphanie AQUIN, Louis-Philippe VEZINA, Marc-André D'AOUST
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Patent number: 9657305Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.Type: GrantFiled: November 4, 2015Date of Patent: May 23, 2017Assignees: Medicago Inc., Centre National de la Recherche Scientifique, Universite De RouenInventors: Thomas Paccalet, Muriel Bardor, Christophe Rihouey, Véronique Gomord, Loïc Faye, Patrice Lerouge, Stéphanie Aquin, Louis-Philippe Vezina, Marc-André D'Aoust
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Publication number: 20170088848Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plant lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: ApplicationFiled: September 2, 2016Publication date: March 30, 2017Applicant: Medicago Inc.Inventors: Marc-Andre D'AOUST, Manon COUTURE, Frederic ORS, Sonia TREPANIER, Pierre-Olivier LAVOIE, Michele DARGIS, Louis-Philippe VEZINA, Nathalie LANDRY
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Patent number: 9546375Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of a novel influenza HA protein in plants and its purification The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding improved influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: GrantFiled: July 15, 2009Date of Patent: January 17, 2017Assignee: MEDICAGO INC.Inventors: Manon Couture, Louis-Philippe Vezina, Nathalie Landry
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Patent number: 9492528Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA of type A/California/04/09 in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein of type A/California/04/09 and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA of type A/California/04/09 as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: GrantFiled: July 2, 2009Date of Patent: November 15, 2016Assignee: MEDICAGO INC.Inventors: Marc-Andre D'Aoust, Manon Couture, Frédéric Ors, Sonia Trepanier, Pierre-Olivier Lavoie, Michèle Dargis, Louis-Philippe Vezina, Nathalie Landry
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Patent number: 9458470Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plant lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: GrantFiled: January 23, 2013Date of Patent: October 4, 2016Assignee: MEDICAGO INC.Inventors: Marc-Andre D'Aoust, Manon Couture, Frederic Ors, Sonia Trepanier, Pierre-Olivier Lavoie, Michele Dargis, Louis-Philippe Vezina, Nathalie Landry
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Patent number: 9452210Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plant lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: GrantFiled: January 4, 2013Date of Patent: September 27, 2016Assignee: MEDICAGO INC.Inventors: Marc-Andre D'Aoust, Manon Couture, Frederic Ors, Sonia Trépanier, Pierre-Olivier Lavoie, Michele Dargis, Louis-Philippe Vézina, Nathalie Landry
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Publication number: 20160145664Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.Type: ApplicationFiled: November 4, 2015Publication date: May 26, 2016Applicants: Medicago Inc., Centre National de la Recherche Scientifique, Universite De RouenInventors: Thomas PACCALET, Muriel BARDOR, Christophe RIHOUEY, Véronique GOMORD, Loïc FAYE, Patrice LEROUGE, Stéphanie AQUIN, Louis-Philippe VEZINA, Marc-André D'AOUST
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Publication number: 20160122777Abstract: A method of producing a virus like particle (VLP) in a plant comprising modified hemagglutinin is provided. The method comprises introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a modified influenza hemagglutinin (HA) protein into the plant, or portion of the plant, the modified HA protein comprises a modified proteolytic loop. Followed by incubating the plant or portion of the plant under conditions that permit the expression of the nucleic acids, thereby producing the VLP. The modified proteolytic loop may comprise one or more protease cleavage sites exhibiting reduced or abolished cleavage by a protease. The nucleotide sequence encoding the HA may be selected from the group consisting of B HA, C, H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15, and H16. Also described is a virus like particle (VLP) produced by the method, and plants expressing the VLP.Type: ApplicationFiled: March 28, 2014Publication date: May 5, 2016Inventors: Manon COUTURE, Marc-Andre D'AOUST, Louis-Philippe VEZINA
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Patent number: 9212372Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.Type: GrantFiled: February 9, 2007Date of Patent: December 15, 2015Assignees: Medicago Inc., Centre National de la Recherche Scientifique, Université De RouenInventors: Thomas Paccalet, Muriel Bardor, Christophe Rihouey, Véronique Gomord, Loïc Faye, Patrice Lerouge, Stéphanie Aquin, Louis-Philippe Vezina, Marc-André D'Aoust
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Publication number: 20150351326Abstract: It is provided a plant infiltration device for infiltrating plants with an inoculum and method of using same, the device comprising a frame having one or more tray racks adapted for receiving at least one tray of plants to be infiltrated; an automated manipulation mechanism mounted to the frame and operable to displace the plant trays within the device; a plurality of inoculum reservoirs mounted to the frame and containing one or more inoculum fluids; an infiltration tank mounted to the frame and within which the plant trays are received when disposed in the infiltration position; and a vacuum generating device in communication with the infiltration tank to apply a negative pressure within the infiltration tank, thereby opening pores of the plant leaves immersed in the inoculum within the infiltration tank and causing the inoculum fluid to infiltrate into the plant leaves.Type: ApplicationFiled: February 15, 2013Publication date: December 10, 2015Inventors: Marc-Andre D'AOUST, Nicole BECHTOLD, Luc LAURIN, Louis-Philippe VEZINA, Normand DUBUC
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Publication number: 20150225462Abstract: A method of producing a picornavirus-like particle (PVLP) in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, portion of the plant, or a plant cell. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding a polyprotein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more protease. The plant, portion of the plant, or plant cell is incubated under conditions that permit the expression of the nucleic acids, thereby producing the PVLP. A PVLP comprising the polyprotein is also provided.Type: ApplicationFiled: August 29, 2013Publication date: August 13, 2015Inventors: Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Manon Couture, Lucie Poulin, Louis-Philippe Vezina
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Publication number: 20150218579Abstract: A method of increasing the yield, stability, or both of an acid sensitive protein in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, or portion of the plant. The first nucleic acid comprises a first regulatory region active in the plant and operatively linked to a nucleotide sequence encoding the acid sensitive protein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a channel protein, for example but not limited to a proton channel protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby increasing the yield of the acid sensitive protein when compared to the yield of the acid sensitive protein produced in the plant or portion of the plant produced under the same conditions, and in the absence of the proton channel protein.Type: ApplicationFiled: January 26, 2015Publication date: August 6, 2015Inventors: Marc-Andre D'AOUST, Manon COUTURE, Louis-Philippe VEZINA
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Publication number: 20150181812Abstract: A plant tray for receiving and retaining one or more plant pots includes a plant-receiving tray for use in a high-throughput plant infiltration process used to produce recombinant proteins. The plant tray described has a base retaining tray with an upper surface defining cavities for receiving the plant pots, and a top plate displaceably mounted on the base retaining tray and having apertures, the apertures defining a channel with the corresponding cavities of the bottom tray when aligned. The top plate is displaceable between a locked operative position wherein the apertures and the corresponding cavities are partially misaligned, thereby locking the plant pots in between the top plate and the base retaining tray.Type: ApplicationFiled: May 28, 2013Publication date: July 2, 2015Applicant: MEDICAGO INC.Inventors: Louis-Philippe Vezina, Nicole Bechtold, Luc Laurin