Patents by Inventor Louis-Philippe Vezina
Louis-Philippe Vezina has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20150104480Abstract: A method of producing a virus like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, or portion of the plant. The first nucleic acid comprises a first regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a structural virus protein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a channel protein, for example but not limited to a proton channel protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.Type: ApplicationFiled: September 28, 2012Publication date: April 16, 2015Inventors: Marc-Andre D'Aoust, Manon Couture, Louis-Philippe Vezina
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Publication number: 20140227322Abstract: A method of producing a virus like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid into the plant, or portion of the plant. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding a native rabies virus structural protein. The nucleotide sequence may further comprise one or more than one amplification element. Optionally, a second nucleic acid might be introduced into the plant, or portion of the plant. The second nucleic acid comprising a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a matrix protein, for example but not limited to a rabies matrix protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.Type: ApplicationFiled: June 13, 2012Publication date: August 14, 2014Applicant: MEDICAGO INC.Inventors: Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Louis-Philippe Vezina, Manon Couture
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Patent number: 8771703Abstract: The present invention provides a recombinant soluble trimeric hemagglutinin (rHA) protein comprising a hemagglutinin ectodomain and an oligomerization domain. The rHA is produced as a soluble homotrimer, and may further comprises a signal peptide and/or an endoplasmic reticulum (ER) retention signal. The invention is also directed to nucleic acids encoding the rHA of the invention, as well as vectors and chimeric constructs comprising the nucleic acid. Methods of producing the rHA are also provided. The rHA described herein may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: GrantFiled: July 7, 2009Date of Patent: July 8, 2014Assignee: Medicago Inc.Inventors: Manon Couture, Nathalie Landry, Louis-Philippe Vezina, Michéle Dargis
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Publication number: 20140024104Abstract: Methods of recovering plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, loosening the cell wall to produce a plant or plant matter having a loosened cell wall, thus allowing the release of apoplastic content through the cell wall to produce an apoplastic content fraction from the plant or plant matter, and recovering the apoplastic content fraction. The apoplastic content fraction comprises plant-derived proteins or suprastructure proteins.Type: ApplicationFiled: March 23, 2012Publication date: January 23, 2014Applicant: MEDICAGO INC.Inventors: Manon Couture, Dany Paquet, Louis-Philippe Vezina
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Publication number: 20130344100Abstract: A method of producing a virus like particle (VLP) in a plant, and compositions comprising VLPs, are provided. The method involves introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a chimeric nucleotide sequence encoding, in series, an ectodomain from a virus trimeric surface protein or fragment thereof, fused to an influenza transmembrane domain and cytoplasmic tail, into the plant, or portion of the plant, the ectodomain is from a non-influenza virus trimeric surface protein and heterologous with respect to the influenza transmembrane domain, and the cytoplasmic tail. The plant or portion of the plant are incubated under conditions that permit the expression of the nucleic acid, thereby producing the VLP. A VLP produced by this method are also provided.Type: ApplicationFiled: December 22, 2011Publication date: December 26, 2013Applicant: MEDICAGO INC.Inventors: Marc-Andre D'Aoust, Manon Couture, Pierre-Olivier Lavoie, Louis-Philippe Vezina
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Publication number: 20130295609Abstract: A plant expression system and methods for expressing a protein of interest in a plant are provided. The plant expression system comprises a first nucleic acid sequence regulatory region sequence, operatively linked with a one or more than one comovirus enhancer, a nucleotide sequence of interest, one or more than one geminivirus amplification elements, and a second nucleic acid encoding a geminivirus replicase. The method of producing a protein of interest in a plant, involves introducing the plant expression system into a plant, or portion of the plant, and incubating the plant or the portion of the plant under conditions that permit the expression of the nucleotide sequence and producing the protein of interest.Type: ApplicationFiled: November 3, 2011Publication date: November 7, 2013Applicant: MEDICAGO INC.Inventors: Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Louis-Philippe Vezina
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Patent number: 8519113Abstract: This invention comprises a combined transgene/virus vector system for the expression of heterologous proteins in plants. While exemplified with respect to the bipartite RNA plant virus, Cowpect mosaic virus (CPMV), other bipartite viral systems may be used to advantage according to the method, system and composition described in detail herein.Type: GrantFiled: May 22, 2006Date of Patent: August 27, 2013Assignee: Plant Bioscience LimitedInventors: George Lomonossoff, Li Liu, Carmen Canizares, Marc-Andre D'Aoust, Louis-Philippe Vezina
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Publication number: 20130067807Abstract: Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.Type: ApplicationFiled: September 21, 2010Publication date: March 21, 2013Applicant: MEDICAGO INC.Inventors: Louis-Philippe Vezina, Manon Couture, Dany Paquet, Michele Dargis, Marc-Andre D'Aoust
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Publication number: 20120189658Abstract: A method for synthesizing chimeric influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of chimeric influenza HA in a plant or a portion of a plant. The invention is also directed towards a VLP comprising chimeric influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding chimeric influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: ApplicationFiled: June 25, 2010Publication date: July 26, 2012Applicant: Medicago Inc.Inventors: Manon Couture, Michele Dargis, Pierre-Olivier Lavoie, Louis-Philippe Vezina, Marc-Andre D'Aoust
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Publication number: 20120178149Abstract: Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction.Type: ApplicationFiled: September 21, 2010Publication date: July 12, 2012Applicant: MEDICAGO INC.Inventors: Louis-Philippe Vezina, Manon Couture, Dany Paquet, Michele Dargis, Marc-Andre D'Aoust
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Publication number: 20110312095Abstract: The present invention provides methods and constructs for increasing recombinant protein production in plants during dehydration stress. The invention includes nucleic acids, inducible expression systems, and methods for using same for increasing recombinant protein production in plants and plant protection.Type: ApplicationFiled: January 9, 2009Publication date: December 22, 2011Inventors: Serge Laberge, Souad Azelmat, Marc-Andre D'Aoust, Pierre-Olivier Lavoie, Jean Cloutier, Yves Castonguay, Louis-Philippe Vezina
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Publication number: 20110293650Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA of type A/California/04/09 in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein of type A/California/04/09 and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA of type A/California/04/09 as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: ApplicationFiled: July 2, 2009Publication date: December 1, 2011Applicant: MEDICAGO INC.Inventors: Marc-Andre D'Aoust, Manon Couture, Frédéric Ors, Sonia Trepanier, Pierre-Olivier Lavoie, Michèle Dargis, Louis-Philippe Vezina, Nathalie Landry
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Publication number: 20110191915Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of a novel influenza HA protein in plants and its purification The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding improved influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: ApplicationFiled: July 15, 2009Publication date: August 4, 2011Inventors: Manon Couture, Louis-Philippe Vezina, Nathalie Landry
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Publication number: 20110104753Abstract: The present invention provides a recombinant soluble trimeric hemagglutinin (rHA) protein comprising a hemagglutinin ectodomain and an oligomerization domain. The rHA is produced as a soluble homotrimer, and may further comprises a signal peptide and/or an endoplasmic reticulum (ER) retention signal. The invention is also directed to nucleic acids encoding the rHA of the invention, as well as vectors and chimeric constructs comprising the nucleic acid. Methods of producing the rHA are also provided. The rHA described herein may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: ApplicationFiled: July 7, 2009Publication date: May 5, 2011Applicant: MEDICAGO INC.Inventors: Manon Couture, Nathalie Landry, Louis-Philippe Vezina, Michéle Dargis
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Publication number: 20110008837Abstract: A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNT1-GalT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNT1) fused to a catalytic domain of beta-1,4galactosyltransferase (GalT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.Type: ApplicationFiled: June 13, 2008Publication date: January 13, 2011Inventors: Marc-Andre D-Aoust, Louis-Philippe Vezina, Stephanie Aquin, Estelle Marquet-Blouin, Muriel Bardor, Veronique Gomord, Christophe Rihouey, Thomas Paccalet, Carole Burel, Loic Faye, Patrice Lerouge, Christophe Sourrouille
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Publication number: 20100310604Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: ApplicationFiled: January 12, 2009Publication date: December 9, 2010Applicant: MEDICAGO INC.Inventors: Marc-André D'Aoust, Manon Couture, Frédéric Ors, Sonia Trépanier, Pierre-Olivier Lavoie, Michéle Dargis, Louis-Philippe Vézina, Nathalie Landry
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Publication number: 20100251417Abstract: A method for synthesizing a protein of interest within a plant or a portion of a plant is provided. The method involves introducing one or more than one nucleic acid sequence encoding a protein of interest operatively linked with a regulatory region obtained from a photosynthetic gene that is active in the plant, in a transient manner. The plant is then maintained under conditions that permit the nucleic acid sequence encoding the protein of interest to be expressed in the plant or the portion of the plant. The plant may be pruned prior to the introducing one or more than one nucleic acid sequence.Type: ApplicationFiled: June 13, 2008Publication date: September 30, 2010Inventors: Marc-Andre D'Aoust, Julie Belles-Isles, Nicole Bechtold, Michele Martel, Pierre-Olivier Lavoie, Louis-Philippe Vezina
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Publication number: 20100239610Abstract: A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.Type: ApplicationFiled: July 11, 2008Publication date: September 23, 2010Applicant: MEDICAGO INC.Inventors: Marc-André D'Aoust, Manon Couture, Frédéric Ors, Sonia Trépanier, Pierre-Olivier Lavoie, Michéle Dargis, Louis-Philippe Vézina, Nathalie Landry
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Publication number: 20090318377Abstract: A cholesterol-lowering preparation comprising medicagenic acid saponin is disclosed. The amount of medicagenic acid saponin in the preparation is greater than 50% by weight to produce the cholesterol-lowering effect in an animal. A method of purifying a preparation of at least 30% medicagenic acid saponin is also disclosed. The preparation may be purified from alfalfa plants and used as a treatment for lowering cholesterol and triglycerides in an animal, in particular a human.Type: ApplicationFiled: July 13, 2007Publication date: December 24, 2009Applicant: MEDICAGO INC.Inventors: Louis-Philippe Vezina, Nathalie Landry, Joelle Pelletier, Sylvain Savard
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Publication number: 20090312240Abstract: A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated a protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5 Ac synthase, CMP-Neu5Ac transporter and a sialyltransferase.Type: ApplicationFiled: February 9, 2007Publication date: December 17, 2009Inventors: Thomas Paccalet, Muriel Bardor, Chirstophe Rihouey, Véronique Gomord, Loïc Faye, Patrice Lerouge, Stéphanie Aquin, Louis-Philippe Vezina, Marc-André D'Aoust