Abstract: Disclosed herein is a hinge antibody capable of being selectively activated in a target cell or tissue to treat a condition therein. The hinge antibody includes a functional antibody, two inhibitory domains and four cleavable linkers. The functional antibody is capable of treating the condition in an activated state, and has two light chains and two heavy chains. Each inhibitory domain includes a hinge domain of an immunoglobulin and consists of two peptide arms. Each cleavable linker includes a peptide substrate cleavable by an enzyme specifically or highly expressed in the target cell or tissue, and connects one of the peptide arms of the inhibitory domains to the N-terminal of one of the light chains and heavy chains of the functional antibody. Also disclosed herein are methods for preparing and using this hinge antibody.

Abstract: The present invention provides a method for preparing an isolated eukaryotic cell which presents an anti-polyethylene glycol (PEG) antibody on a cell membrane. The present invention also provides a method for a quantitative analysis of a polyethylene glycol (PEG) by said anti-PEG antibody expressing cell. The cell-based quantitative analysis of the present prevention could sensitively quantify free PEG and PEG-modified macromolecules (proteins, nanoparticles and liposomes) as sensitive as nano-gram level.

Abstract: The invention provides a method of high-throughput sorting of high expression protein-producing cell, which utilizes linking a protein and a transmembrane domain with a self-processing cleavage site and regulating the secretion of the protein or expression of protein on the cell membrane by adding self-processing cleavage enzyme inhibitor. Then, the high expression cell line can be high-throughput sorted by a detection technique. The invention also provides a recombinant nucleotide sequence and a vector used in the method and a cell sorted by the method.

Abstract: Listings and reviews of listings can be processed to identify descriptive attributes for locations associated with the listings. To do this, a corpus of words is generated for various locations based on listings in the locations and reviews of those listings. An expected frequency, and per-location frequency for each word is determined. These numbers are in turn used to determine a number of high frequency listing locations, and a number of below expected frequency listing locations for each word. Based on a comparison of the number of high frequency listing locations and the number of below expected frequency listing locations of a word with an attribute reference number, the word can be identified either as an attribute that is likely descriptive of the location, or not.

Abstract: A configurable wavelength-selective cross-connect (WSXC) device having an array of optical ports in which at least some of the ports are configurable to operate as common ports or as plural ports. The configurable WSXC device includes a MEMS mirror array whose mirrors are tiltable in a manner that enables, e.g., reconfigurations of the configurable WSXC device that result in a change of the number of common ports therein. In one possible configuration, the configurable WSXC device can operate as 1×M1 wavelength-selective switch, where M1+1?P, and P is the total number of optical ports in the array of optical ports. In another possible configuration, the configurable WSXC device can operate as a bank of n 1×M2 wavelength-selective switches, where n is a positive integer greater than one, and n(M2+1)?P.

Abstract: A method of preparing an epitope for a secondary antibody detected protein tag, comprising: constructing a expressing vector, comprising a nucleotide sequence for encoding the secondary antibody detected protein tag comprising at least one epitope selected from at least one primary antibody which is detected by corresponding secondary antibody; expressing the vector in a host cell; and obtaining the secondary antibody detected protein tag which is expressed in the host cell. Further, a method for constructing a protein molecular weight marker ladder, comprising constructing a plurality of recombinant protein tags with different molecular weights, wherein each the recombinant protein tag comprises at least one epitope of primary antibody. Besides, a secondary antibody detected protein tag comprises at least two peptide sequences of epitopes selected from primary antibodies of at least two different species.

Abstract: A configurable wavelength-selective cross-connect (WSXC) device having an array of optical ports in which at least some of the ports are configurable to operate as common ports or as plural ports. The configurable WSXC device includes a MEMS mirror array whose mirrors are tiltable in a manner that enables, e.g., reconfigurations of the configurable WSXC device that result in a change of the number of common ports therein. In one possible configuration, the configurable WSXC device can operate as 1×M1 wavelength-selective switch, where M1+1?P, and P is the total number of optical ports in the array of optical ports. In another possible configuration, the configurable WSXC device can operate as a bank of n 1×M2 wavelength-selective switches, where n is a positive integer greater than one, and n(M2+1)?P.

Abstract: The invention provides a method of high-throughput sorting of high expression protein-producing cell, which utilizes linking a protein and a transmembrane domain with a self-processing cleavage site and regulating the secretion of the protein or expression of protein on the cell membrane by adding self-processing cleavage enzyme inhibitor. Then, the high expression cell line can be high-throughput sorted by a detection technique. The invention also provides a recombinant nucleotide sequence and a vector used in the method and a cell sorted by the method.

Abstract: A semiconductor annealing method and system uses a vacuum pump to produce a vacuum environment in the annealing chamber to thereby remove undesired gas element influences. A control system obtains pressure and temperature measurements from the annealing chamber to control operation of the heating elements and vacuum pump to thereby maintain process integrity.

Abstract: The present invention provides an auto-developing and regularly-weighted protein molecular weight marker kit, comprising: (a) a plurality of recombinant proteins having formula (I), (B)m-A-(C)n??(I), wherein A is a polypeptide of SEQ ID NO: 1, B and C are independently any mutually identical or different polypeptides with the value of molecular weight being a multiple of 5, and m and n is independently 0 or any integer larger than 0; and (b) one or more solvents for stabilizing the recombinant proteins. The present invention also provides a method for preparing the auto-developing and regularly-weighted protein molecular weight marker kit.

Abstract: A loudspeaker, which includes a ferrite core, a voice coil, and a movable membrane. The voice coil is wound around the core and cooperatively defining an exciting device. The movable membrane is disposed in close proximity to the exciting device with a gap formed thereinbetween. The loudspeaker is constructed without permanent magnets.

Abstract: An earphone integrated with a microphone includes a housing, a speaker, a microphone (audio-recording device), and a supporting member. The housing has an audio-output hole formed thereon a receiving space formed therein. The speaker and the microphone are arranged in the receiving space. The supporting member is arranged in the housing. One end of the microphone is mounted on the supporting member for suspending the microphone in the receiving space. A buffering space is formed around the microphone. Thus, the earphone can achieve anti-noise effect in providing improved communication quality.

Abstract: A portable information product comprising: an amplifying circuit; a data processing device having a main circuit module having a microprocessor and a plurality of memories for processing data thereof and forming an output signal from the data, and having a storage device electrically connecting to the main circuit module for storing data of the data processing device, and having an application circuit module including a charging circuit module; a built-in projection device disposed in the data processing device the projection device having a light source and a projector, the projector having a imaging unit and a lens; a display device disposed in the data processing device; a switching circuit module disposed in the data processing device and electrically connecting to the projection device and the display device; a photograph module disposed in the data processing device, and electrically connecting between the lens and the switching circuit module.

Abstract: The present invention provides a method for preparing an isolated eukaryotic cell which presents an anti-polyethylene glycol (PEG) antibody on a cell membrane. The present invention also provides a method for a quantitative analysis of a polyethylene glycol (PEG) by said anti-PEG antibody expressing cell. The cell-based quantitative analysis of the present prevention could sensitively quantify free PEG and PEG-modified macromolecules (proteins, nanoparticles and liposomes) as sensitive as nano-gram level.

Abstract: The present invention provides a method and apparatus for enhancing webpage browsing. The method of the invention includes a predicting step which predicts, for a first block in the webpage, a subsequent second block, based on a browsing history of blocks of webpages by at least one user; and a link generating step of automatically generating a link pointing to the second block based on a result of the predicting step. The apparatus includes: predicting means configured to predict, for a first block in the webpage, a subsequent second block, based on a browsing history of blocks of web pages by at least one user; and link generating means configured to automatically generate a link pointing to the second block based on a result of the predicting means.

Abstract: The present invention provides an auto-developing and regularly-weighted protein molecular weight marker kit, comprising: (a) a plurality of recombinant proteins having formula (I), (B)m-A-(C)n??(I), wherein A is a polypeptide of SEQ ID NO: 1, B and C are independently any mutually identical or different polypeptides with the value of molecular weight being a multiple of 5, and m and n is independently 0 or any integer larger than 0; and (b) one or more solvents for stabilizing the recombinant proteins. The present invention also provides a method for preparing the auto-developing and regularly-weighted protein molecular weight marker kit.

Abstract: The invention provides a recombinant nucleotide sequence, including the sequence of SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 or SEQ ID No. 4, wherein the recombinant nucleotide sequence encodes an anti-polyethylene glycol recombinant single chain membrane antibody.

Abstract: The instant disclosure relates to a loudspeaker, which includes a ferrite core, a voice coil, and a movable membrane. The voice coil is wound around the core and cooperatively defining an exciting device. The movable membrane is disposed in close proximity to the exciting device with a gap formed thereinbetween. The loudspeaker constructed without permanent magnets takes up less space and is more cost effective. Thus, the loudspeaker has a broader application range.

Abstract: A method for diagnosis of diseases adopted on an electronic stethoscope which includes at least two sound receiving portions, a noise control portion, a processing portion, a data portion and an output portion. The method includes: first, the sound receiving portions receive sound signals issued from a patient's lungs included external noises; next, the sound signals are sent to the noise control portion which eliminates the external noise, and the processing portion to be overlapped and intensified; then characteristic values are retrieved from the sound signals to be compared with disease sound signal data in the data portion; finally the output portion outputs a diseases judgment result. Thus the electronic stethoscope can perform automatic interpretation of diseases to reduce human erroneous diagnostic judgment. Users also can get preliminary understanding of their body conditions when doctors are absent.

Abstract: An earphone integrated with a microphone includes a housing, a speaker, a microphone (audio-recording device), and a supporting member. The housing has an audio-output hole formed thereon a receiving space formed therein. The speaker and the microphone are arranged in the receiving space. The supporting member is arranged in the housing. One end of the microphone is mounted on the supporting member for suspending the microphone in the receiving space. A buffering space is formed around the microphone. Thus, the earphone can achieve anti-noise effect in providing improved communication quality.