Abstract: Provided herein are polypeptides comprising a therapeutic targeted for delivery to an organ or tissue, and uses thereof.

Abstract: The present invention relates to a method for generating a result image. The method comprises the steps of: acquiring a STED image of a sample, the STED image comprising pixels; calculating Fourier coefficients of arrival times for the pixels of the image, resulting in real coefficients representing a first image and in imaginary coefficients representing a second image; deriving an intensity image from the STED image; applying a spatial filter to the first image, the second image and the intensity image, resulting in a filtered first image, a filtered second image, and filtered intensity image, respectively; calculating an image G based on the filtered first image and the filtered intensity image; calculating an image S based on the filtered second image and the filtered intensity image; and calculating a result image based on the image G and the image S.

Abstract: A detector device for a microscope includes a multi-element photodetector having a plurality of photodetector elements arranged in a photodetector array. Each photodetector element is configured to output a detector signal upon receiving light. The plurality of photodetector elements is arranged in one or more photodetector groups. Each photodetector group has a signal combiner configured to combine the detector signals of the photodetector elements into a collective output signal of the photodetector group to reduce a dead time thereof. In a case of only one photodetector group, the multi-element photodetector includes an optical distributor configured to distribute the light across the photodetector group; or in a case of more than one photodetector group, the photodetector groups differ from each other with respect to a density at which the photodetector elements are arranged in the respective photodetector group.

Abstract: Systems, apparatus, articles of manufacture, and methods are disclosed for inductors of voltage regulators that are built into and/or around mounting holes of a printed circuit board. An example apparatus includes a printed circuit board that includes a plurality of layers and a mounting hole extending through the plurality of layers, and an inductor at least partially in the mounting hole between two or more of the plurality of layers.

Abstract: A method of modifying a surface of a three-dimensional (3D) article includes immersing at least one part of the 3D article in a buffered solution of functionalized peptides, allowing reaction between the functionalized peptides and reactive groups on the surface of the 3D article; and washing the at least one part of the 3D article to remove unreacted functionalized peptides. The surface-modified 3D article includes a plurality of peptides covalently bonded to the surface of the 3D article via a cysteine bridge. The surface-modified 3D article can be used as a scaffold for the formation of biological tissue or bodily implants.

Abstract: A processor for lifetime-based unmixing in fluorescence microscopy is configured to acquire an image having a plurality of pixels, each pixel providing information on photon count and photon arrival times, generate a phasor plot that is a vector space representation of the image, partition the image into image segments, evaluate the image segments according to total photon counts of the corresponding subsets of pixels, and execute a lifetime classification by selecting an image segment having a largest total photon count, determining a region of interest in the image encompassing the image segment, determining a phasor subset in the phasor plot corresponding to the region of interest, and generating a lifetime class including a set of image segments corresponding to the phasor subset. A plurality of lifetime classes is generated by iteratively executing the lifetime classification. The processor is configured to perform lifetime-based unmixing using the life-time classes.

Abstract: Provided herein are methods which alter the mechanical and biological properties of polymeric materials. Also provided are compositions comprising the polymeric materials having said properties.

Abstract: The present disclosure provides devices comprising a therapeutic agent bound to a printed three-dimensional structure. Ink formulations for three-dimensional printing are also disclosed. Additionally, provided herein are methods for manufacturing devices and uses there e.g., in treating a condition in a subject in need thereof. The devices may be coated with therapeutic agents, such as those that promo bone growth, and/or seeded with cells to generate devices for use in tissue replacement and grafting.

Abstract: Using an interface and contacts database according to the present technology, a user may add a new contact to their contacts database along with information about how the user met the new contact and/or information about other contacts who are known to or associated with the new contact.

Abstract: Provided herein are methods which alter the mechanical and biological properties of polymeric materials. Also provided are compositions comprising the polymeric materials having said properties.

Abstract: An advanced manufactured interpenetrating polymer network (AM-IPN) comprising: a primary polymer network; a secondary polymer network, wherein the secondary polymer network is bonded to the primary polymer network via one or more crosslinks, wherein one or more of the primary polymer network, the secondary polymer network and the one or more crosslinks are printed using a synthetic bioink is disclosed. Methods of making and using are also disclosed.

Abstract: The present disclosure provides devices comprising a therapeutic agent bound to a printed three-dimensional structure. Ink formulations for three-dimensional printing are also disclosed. Additionally, provided herein are methods for manufacturing devices and uses there e.g., in treating a condition in a subject in need thereof. The devices may be coated with therapeutic agents, such as those that promo bone growth, and/or seeded with cells to generate devices for use in tissue replacement and grafting.

Abstract: A bone regeneration material has a cotton-wool like structure formed of a plurality of electrospun fibers that contain bound BMP-2 through ?-TCP binding peptide. The electrospun biodegradable fiber contains 25-65 vol % of ?-TCP particles distributed in the fiber such that a portion of the ?-TCP particles is exposed on a surface of the electrospun fiber and the remaining portion of the ?-TCP particles is buried in the fiber. ?-TCP binding peptides that are fused with BMP-2 are bound to the ?-TCP particles so that BMP-2 is tethered to ?-TCP particles on the surface of the fibers. Upon implantation of the bone regeneration material in a bone defect site of a human body, BMP-2 that are tethered to ?-TCP particles on the surface of the bone regeneration material promotes proliferation and differentiation of cells at the bone defect site.

Abstract: The invention relates to trimeric polypeptide complex comprising three monomer polypeptides wherein each monomer polypeptide comprises an anti-4-1BB specific agonistic single-chain antibody fragment (scFv), a homotrimerization domain and a polypeptide region which is capable of specifically binding to a tumor associated antigen. The invention also relates to said trimeric polypeptide complexes for use in the treatment of cancer.

Abstract: It is provided a method, device and system to facilitate the design or and architecture of a computer project and its subsequent automatic implementation, based on a hand-written/drawn draft (e.g. on paper). This draft directly becomes the tool that will be used, automatically, as the basis for the final computer project implementation.

Abstract: A glass/quartz composite structure may comprise aggregate including glass grit, nano-glass crystals, and/or amorphous silica that may be produced from crystalline quartz. The aggregate may be in an amount greater than any other single material by weight of the composite structure, which may further include quartz powder and/or alumina as well as a binding resin. The glass/quartz composite structure may be devoid of crystalline silica. The structure may be formed into a 1.2-1.5 cm thick slab for countertops using standard cabinet perimeter support. The slab may be made by mixing the aggregate, the quartz powder and/or alumina, and the binding resin, pouring the mixture in a mold, and compacting the mixture in the mold. Specific natural mineral components, decorative chips, and/or wet mixture pieces may be added to the composite structure to provide aesthetics of specific natural stones.

Abstract: An advanced manufactured transwell (AM-transwell), the AM-transwell comprises: a lower chamber; an upper chamber; a membrane disposed between the lower chamber and the upper chamber; and one or more legs. The one or more legs form at least a portion of the lower chamber. One or more of the lower chambers, the upper chamber, the membrane and the one or more legs is printed using a synthetic bioink. Methods for making and using the AM-transwell are also disclosed.

Abstract: Provided herein are polypeptides that include one or more ?-tricalcium phosphate (?TCP)-binding sequence(s) and uses thereof.

Abstract: A controller for an imaging device is configured to control an optical detection unit to receive fluorescence light emitted by first and second fluorophore species excited by first and second excitation light, respectively, to separate the fluorescence light into at least two spectral detection channels corresponding to two wavelength bands, respectively. A first wavelength band includes an emission spectrum of the first fluorophore species. A second wavelength band includes an emission spectrum of the second fluorophore species. The controller is further configured to control the optical detection unit to detect photon arrival times of the fluorescence light relative to light pulses of the second excitation light, to determine a temporal correlation between the light pulses and the photon arrival times, and to determine a first number and a second number of photons received in the first spectral detection channel and/or the second spectral detection channel based on the temporal correlation.

Abstract: Provided herein are polypeptides that include one or more ?-tricalcium phosphate (?TCP)-binding sequence(s) and uses thereof.