Abstract: A nanostructural sensing substrate includes indium tin oxide (ITO) film coated upstanding silicon nanowires (ITO/USNWs). The ITO/USNWs are fabricated by coating an ITO film on USNWs, the density of which has been reduced using a facile Ag-assisted chemical etching method. Furthermore, the bioreceptor modified ITO/USNWs are developed to serve as the sensing substrate of the EGFETs device for label-free diagnosis of biomarker related diseases, such as Alzheimer's disease (AD), acute myocardial infarction, coronary artery disease (CAD), hepatic encephalopathy, lung fibrosis, Cushing's syndrome and cancers. The ITO-coated-USNWs are also used in nano-featured cell based biosensors (CBB) for electrically quantitative evaluation of drug release.

Abstract: Described is a method of calibrating a camera. The method comprises defining an area observed in a field of view (FOV) of the camera as a region of interest (ROI). The method includes selecting a plurality of physical reference points in said ROI and obtaining for each reference point physical position coordinates. The method also includes obtaining from said camera FOV or camera image data pixel locations for each point of reference and pairing each pixel location with the physical position coordinates of its respective reference point. Then, a relationship is derived from the paired pixel locations and physical position coordinates to enable a selected or identified pixel location in the camera FOV or camera image data to be transformed to physical position coordinates for a corresponding physical location in said ROI.

Abstract: Compositions and methods for fixing cells or nuclei or extracellular vesicles with dithiobismaleimidoethane (DTME) and subsequent reverse transcription in the cells or nuclei or extracellular vesicles are provided.

Abstract: Methods and compositions for nucleotide sequencing are provided. In some embodiments, click chemistry is used to link barcoding oligonucleotides to DNA fragments comprising adapters introduced by a transposase.

Abstract: Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.

Abstract: A polymer composition for producing gel extruded articles is described. The polymer composition contains polyethylene particles combined with a plasticizer and one or more strength enhancing additives. Polymer articles made in accordance with the present disclosure have enhanced strength properties. In one embodiment, the polymer composition is used to form a porous membrane for use as a separator in electronic devices.

Abstract: A polymer composition for producing gel extruded articles is described. The polymer composition contains polyethylene particles combined with a plasticizer and one or more strength enhancing additives. Polymer articles made in accordance with the present disclosure have enhanced strength properties. In one embodiment, the polymer composition is used to form a porous membrane for use as a separator in electronic devices.

Abstract: Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.

Abstract: A refrigerant distributor (4) includes: a box body (42); a refrigerant inlet (41) arranged on an upper surface (421) of the box body (42); liquid exit openings (46) evenly arranged on a lower surface (422) of the box body (42); and end plates arranged at both ends of the box body (42) in a length direction and enclosing the box body (42) from the two ends; wherein, in a height direction from the lower surface (422) of the box body (42) to the upper surface (421) and within a predetermined height range starting from the lower surface (422), a width of the box body (42) increases gradually; and a pre-distributor (3) is arranged inside the box body (42).

Abstract: Methods of making and forming partitions in nanovials are provided.

Abstract: Inventions covered include methods, systems, and compositions for sample processing, involving morphology-adjustable (e.g., tunable on-demand) functionalized particles. In some embodiments, a method can include distributing a set of functionalized particles, in a first morphological state, across a set of partitions; transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state; transitioning the set of functionalized particles, at the set of partitions, from the second morphological state to a third morphological state, and inducing interactions between the set of functionalized particles and a set of targets, within the set of partitions and according to a set of operations with a set of process fluids.

Abstract: Described is a method of calibrating a camera. The method comprises obtaining geographical coordinates of a selected physical point location within an image view of the camera and measuring an angle between an x-axis of a real-world coordinate system passing through said selected point location with respect to true north. The method includes using said obtained geographical coordinates, said measured angle, and projection data derived from characteristics of the camera to derive modified projection data for transforming a two-dimensional pixel coordinate system of the camera image view into a three-dimensional geographical coordinate system for point locations within the image view of the camera.

Abstract: Described is a method of calibrating a camera. The method comprises obtaining geographical coordinates of a selected physical point location within an image view of the camera and measuring an angle between an x-axis of a real-world coordinate system passing through said selected point location with respect to true north. The method includes using said obtained geographical coordinates, said measured angle, and projection data derived from characteristics of the camera to derive modified projection data for transforming a two-dimensional pixel coordinate system of the camera image view into a three-dimensional geographical coordinate system for point locations within the image view of the camera.

Abstract: Described is a method of calibrating a camera. The method comprises defining an area observed in a field of view (FOV) of the camera as a region of interest (ROI). The method includes selecting a plurality of physical reference points in said ROI and obtaining for each reference point physical position coordinates. The method also includes obtaining from said camera FOV or camera image data pixel locations for each point of reference and pairing each pixel location with the physical position coordinates of its respective reference point. Then, a relationship is derived from the paired pixel locations and physical position coordinates to enable a selected or identified pixel location in the camera FOV or camera image data to be transformed to physical position coordinates for a corresponding physical location in said ROI.

Abstract: Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.

Abstract: Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.

Abstract: A polymer composition for producing gel extruded articles is described. The polymer composition contains polyethylene particles combined with a plasticizer and one or more strength enhancing additives. Polymer articles made in accordance with the present disclosure have enhanced strength properties. In one embodiment, the polymer composition is used to form a porous membrane for use as a separator in electronic devices.

Abstract: Barcoding composition and methods involving solid supports having sets of different oligonucleotides that can be decoded to the same identification sequence.