Abstract: A charging station comprising a charging unit and an electrical-energy storage unit is integrated into a trailer, the fixed body of a truck, the interchangeable system of a truck, the interchangeable container of a truck, a van, or a trailer towed by a truck or a car.

Abstract: A measuring device and method according to the invention for registering an ambient temperature Ta of a medical machine including a measuring housing, which is arranged on an outer wall of the machine and in which at least one temperature sensor remote from the machine and at least one temperature sensor (16, 16?) near to the machine are held, wherein the at least one temperature sensor remote from the machine registers a first temperature T1 and the at least one temperature sensor near to the machine registers a second temperature T2, and an evaluation apparatus configured to establish the ambient temperature Ta using a difference between the first temperature T1 registered by the at least one temperature sensor remote from the machine and the second temperature T2 registered by the at least one temperature sensor near to the machine.

Abstract: The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference.

Abstract: The present invention relates to a method for determining the in vivo localization of double-strand breaks in a host cell, comprising incubating a host cell suspected to comprise DNA double-strand breaks and a linear polynucleotide comprising a known sequence, detecting the in vivo insertion sites of said polynucleotide in the genome of said host cell, and assessing the in vivo localization of double-strand breaks. Further envisaged by the present invention is a method for obtaining an endonuclease with altered in vivo specificity. Finally, the present invention is directed to a kit for determining in vivo specificity of an endonuclease.

Abstract: An isocyanate-terminated prepolymer C having an NCO content of from ?26% by mass to ?31.0% by mass is obtainable from the reaction of an isocyanate composition A having a viscosity at 25° C. of from ?60 mPas to ?800 mPas, comprising from ?25% by mass to ?60% by mass MDI and from ?40% by mass to ?75% by mass pMDI, wherein the sum of the amounts of those components is ?100% by mass, with a bifunctional polyester ether polyol B having an OH number of ?200 mg(KOH)/g and ?500 mg(KOH)/g, obtained from the reaction of a dicarboxylic acid/dicarboxylic acid derivative with at least one polyol and with an epoxide, wherein the polyester ether polyol B is used in amounts of from ?1.5% by mass to ?6.0% by mass, based on the sum of the masses of A and B. The invention further provides a PUR/PIR polymer produced from the prepolymer C, its use and composite elements based thereon.

Abstract: A resealable and tamper resistant bag includes a front wall having a first resealable feature, and a back wall having a second resealable feature adapted to form a seal with the first resealable feature. The front and back walls are sealingly connected along at least a pair of sides to define at least part of a holding portion of the bag. A sealed tamper resistant strip portion is formed integrally with the holding portion and is disposed adjacent the first and second resealable features. At least one seam is disposed between the holding portion and the strip portion for allowing the strip portion to be selectively removed, at least in part, from the holding portion by a user. At least one tab is defined by the seam to facilitate the user moving the first and second resealable features from a sealed position to an unsealed position.

Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.

Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.

Abstract: The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference.

Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.

Abstract: A measuring device and method according to the invention for registering an ambient temperature Ta of a medical machine including a measuring housing, which is arranged on an outer wall of the machine and in which at least one temperature sensor remote from the machine and at least one temperature sensor (16, 16?) near to the machine are held, wherein the at least one temperature sensor remote from the machine registers a first temperature T1 and the at least one temperature sensor near to the machine registers a second temperature T2, and an evaluation apparatus configured to establish the ambient temperature Ta using a difference between the first temperature T1 registered by the at least one temperature sensor remote from the machine and the second temperature T2 registered by the at least one temperature sensor near to the machine.

Abstract: A method for grit blasting tubes and for blowing swabs through tubes, automatically sensing that the swab has passed through the tube, and logging the event. Also, various methods are used for inserting the swabs into the tubes efficiently.

Abstract: The present invention relates to the technical field of isocyanates. The present invention provides a method for preparing NCO prepolymers by reacting a stoichiometric excess of an organic isocyanate with an isocyanate-reactive component, the reaction components being mixed together and the mixture being introduced directly into a storage or transport vessel, where it reacts to completion.

Abstract: A process for the preparation of polyricinoleic acid esters comprising the step of reaction of ricinoleic acid with an alcohol component which comprises mono- and/or polyfunctional alcohols having a molecular weight of ?32 g/mol to ?40 g/mol, wherein the reaction is carried out at least partly in the presence of a catalyst. The amount of catalyst, based on the total weight of the ricinoleic acid and the alcohol component, is in a range of from ?10 ppm to ?100 ppm. The reaction is ended when the acid number of the reaction product obtained is ?5 mg of KOH/g to ?100 mg of KOH/g. The invention furthermore relates to a polyurethane polymer, in particular a flexible polyurethane foam, which is obtainable using these polyricinoleic acid esters.

Abstract: An isocyanate-terminated prepolymer C having an NCO content of from ?26% by mass to ?31.0% by mass is obtainable from the reaction of an isocyanate composition A having a viscosity at 25° C. of from ?60 mPas to ?800 mPas, comprising from ?25% by mass to ?60% by mass MDI and from ?40% by mass to 75% by mass pMDI, wherein the sum of the amounts of those components is ?100% by mass, with a bifunctional polyester ether polyol B having an OH number of ?200 mg(KOH)/g and ?500 mg(KOH)/g, obtained from the reaction of a dicarboxylic acid/dicarboxylic acid derivative with at least one polyol and with an epoxide, wherein the polyester ether polyol B is used in amounts of from ?1.5% by mass to ?6.0% by mass, based on the sum of the masses of A and B. The invention further provides a PUR/PIR polymer produced from the prepolymer C, its use and composite elements based thereon.

Abstract: The present invention provides an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase. The present invention further provides a method of stabilizing an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase, and a method of preparing a purified aqueous composition comprising the protein with enzymatic activity of alpha-galactosidase.