Patents by Inventor Mario Ancona

Mario Ancona has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210310952
    Abstract: The stiffness and topology of ultra-small circular DNAs and DNA/peptide hybrids are exploited to create a transducer of enzyme activity with low error rates. The modularity and flexibility of the concept are illustrated by demonstrating various transducers that respond to either specific restriction endonucleases or to specific proteases. In all cases the output is a DNA oligo signal that, as we show, can readily be converted directly to an optical readout, or can serve as input for further processing, for example, using DNA logic or amplification. By exploiting the DNA hairpin (or stem-loop) structure and the phenomenon of strand displacement, an enzyme signal is converted into a DNA signal, in the manner of a transducer. This is valuable because a DNA signal can be readily amplified, combined, and processed as information.
    Type: Application
    Filed: June 16, 2021
    Publication date: October 7, 2021
    Inventors: Mario Ancona, Hieu Bui
  • Patent number: 11067508
    Abstract: The stiffness and topology of ultra-small circular DNAs and DNA/peptide hybrids are exploited to create a transducer of enzyme activity with low error rates. The modularity and flexibility of the concept are illustrated by demonstrating various transducers that respond to either specific restriction endonucleases or to specific proteases. In all cases the output is a DNA oligo signal that, as we show, can readily be converted directly to an optical readout, or can serve as input for further processing, for example, using DNA logic or amplification By exploiting the DNA hairpin (or stem-loop) structure and the phenomenon of strand displacement, an enzyme signal is converted into a DNA signal, in the manner of a transducer. This is valuable because a DNA signal can be readily amplified, combined, and processed as information.
    Type: Grant
    Filed: April 14, 2020
    Date of Patent: July 20, 2021
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Mario Ancona, Hieu Bui
  • Publication number: 20200326283
    Abstract: The stiffness and topology of ultra-small circular DNAs and DNA/peptide hybrids are exploited to create a transducer of enzyme activity with low error rates. The modularity and flexibility of the concept are illustrated by demonstrating various transducers that respond to either specific restriction endonucleases or to specific proteases. In all cases the output is a DNA oligo signal that, as we show, can readily be converted directly to an optical readout, or can serve as input for further processing, for example, using DNA logic or amplification By exploiting the DNA hairpin (or stem-loop) structure and the phenomenon of strand displacement, an enzyme signal is converted into a DNA signal, in the manner of a transducer. This is valuable because a DNA signal can be readily amplified, combined, and processed as information.
    Type: Application
    Filed: April 14, 2020
    Publication date: October 15, 2020
    Inventors: Mario Ancona, Hieu Bui
  • Publication number: 20200017905
    Abstract: Time-resolved nucleic acids include a long-lifetime FRET donor with an emission lifetime of at least one millisecond (such as a terbium complex), configured as a donor in a first FRET process, and at least one fluorescent dye with an emission lifetime of less than 100 nanoseconds configured as an acceptor in the FRET process. They can be configured as photonic wires, hybridization probes or beacons, and/or systems for computing logical operations.
    Type: Application
    Filed: September 24, 2019
    Publication date: January 16, 2020
    Inventors: Igor L. Medintz, Mario Ancona, W. Russ Algar, Melissa M. Massey
  • Publication number: 20200010877
    Abstract: Time-resolved nucleic acids include a long-lifetime FRET donor with an emission lifetime of at least one millisecond (such as a terbium complex), configured as a donor in a first FRET process, and at least one fluorescent dye with an emission lifetime of less than 100 nanoseconds configured as an acceptor in the FRET process. They can be configured as photonic wires, hybridization probes or beacons, and/or systems for computing logical operations.
    Type: Application
    Filed: September 24, 2019
    Publication date: January 9, 2020
    Inventors: Igor L. Medintz, Mario Ancona, W. Russ Algar, Melissa M. Massey
  • Patent number: 10466190
    Abstract: An apparatus and method for producing chemielectric point-sensor systems with increased sensitivity and increased selectivity. The chemielectric sensor system includes a sensor/heater assembly, where the sensor is a chemielectric sensor whose resistance or capacitance changes upon exposure to chemical analytes. The heater functionality applies a programmed sequence of one or more thermal pulses to the sensor to quickly raise its temperature. After each thermal pulse ends the change in resistivity of the sensor is measured. Such data as a function of the pulse time and temperature are recorded and analyzed to determine the chemical composition (selectivity) and concentrations in the ambient vapor by comparison to a library dataset. The sensor operation with fast thermal pulses also allows operation at higher frequencies where the noise is lower and hence sensitivity is improved.
    Type: Grant
    Filed: September 24, 2018
    Date of Patent: November 5, 2019
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Mario Ancona, F. Keith Perkins, Arthur W. Snow
  • Patent number: 10465233
    Abstract: Time-resolved nucleic acids include a long-lifetime FRET donor with an emission lifetime of at least one millisecond (such as a terbium complex), configured as a donor in a first FRET process, and at least one fluorescent dye with an emission lifetime of less than 100 nanoseconds configured as an acceptor in the FRET process. They can be configured as photonic wires, hybridization probes or beacons, and/or systems for computing logical operations.
    Type: Grant
    Filed: March 24, 2017
    Date of Patent: November 5, 2019
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Igor L. Medintz, Mario Ancona, W. Russ Algar, Melissa M. Massey
  • Patent number: 10260086
    Abstract: The invention relates to a nanoscale antenna including a nucleic acid scaffold having a structure selected from the group consisting of a Holliday junction, a star, and a dendrimer; and a plurality of fluorophores attached to the scaffold and configured as a FRET cascade comprising at least three different types of fluorophores including at least one quantum dot, arranged with (a) a plurality of initial donor fluorophores fixed in exterior positions on the structure, (b) a terminal acceptor fluorophore fixed in a central position on the structure, and (c) a plurality of intermediate fluorophores fixed in positions on the scaffold between the initial acceptor fluorophores and the terminal acceptor fluorophores.
    Type: Grant
    Filed: April 13, 2018
    Date of Patent: April 16, 2019
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Mario Ancona, Ellen R. Goldman, Susan Buckhout-White, Igor L. Medintz, Joseph S. Melinger
  • Publication number: 20180230522
    Abstract: The invention relates to a nanoscale antenna including a nucleic acid scaffold having a structure selected from the group consisting of a Holliday junction, a star, and a dendrimer; and a plurality of fluorophores attached to the scaffold and configured as a FRET cascade comprising at least three different types of fluorophores including at least one quantum dot, arranged with (a) a plurality of initial donor fluorophores fixed in exterior positions on the structure, (b) a terminal acceptor fluorophore fixed in a central position on the structure, and (c) a plurality of intermediate fluorophores fixed in positions on the scaffold between the initial acceptor fluorophores and the terminal acceptor fluorophores.
    Type: Application
    Filed: April 13, 2018
    Publication date: August 16, 2018
    Inventors: Mario Ancona, Ellen R. Goldman, Susan Buckhout-White, Igor L. Medintz, Joseph S. Melinger
  • Publication number: 20180171325
    Abstract: A nanoparticle (for example, quantum dot) serves as a substrate for immobilizing enzymes involved in consecutive reactions as a cascade. This results in a significant increase in the rate of catalysis as well as final product yield compared to non-immobilized enzymes.
    Type: Application
    Filed: December 13, 2017
    Publication date: June 21, 2018
    Inventors: Igor L. Medintz, James N. Vranish, Mario Ancona, Kimihiro Susumu, Sebastian A. Diaz
  • Patent number: 9970049
    Abstract: The invention relates to a nanoscale antenna including a nucleic acid scaffold having a structure selected from the group consisting of a Holliday junction, a star, and a dendrimer; and a plurality of fluorophores attached to the scaffold and configured as a FRET cascade comprising at least three different types of fluorophores, arranged with (a) a plurality of initial donor fluorophores fixed in exterior positions on the structure, (b) a terminal acceptor fluorophore fixed in a central position on the structure, and (c) a plurality of intermediate fluorophores fixed in positions on the scaffold between the initial acceptor fluorophores and the terminal acceptor fluorophores.
    Type: Grant
    Filed: April 14, 2015
    Date of Patent: May 15, 2018
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Susan Buckhout-White, Mario Ancona, Ellen R. Goldman, Igor L. Medintz, Joseph S. Melinger
  • Publication number: 20170275677
    Abstract: Time-resolved nucleic acids include a long-lifetime FRET donor with an emission lifetime of at least one millisecond (such as a terbium complex), configured as a donor in a first FRET process, and at least one fluorescent dye with an emission lifetime of less than 100 nanoseconds configured as an acceptor in the FRET process. They can be configured as photonic wires, hybridization probes or beacons, and/or systems for computing logical operations.
    Type: Application
    Filed: March 24, 2017
    Publication date: September 28, 2017
    Inventors: Igor L. Medintz, Mario Ancona, W. Russ Algar, Melissa M. Massey
  • Publication number: 20150293022
    Abstract: The invention relates to a nanoscale antenna including a nucleic acid scaffold having a structure selected from the group consisting of a Holliday junction, a star, and a dendrimer; and a plurality of fluorophores attached to the scaffold and configured as a FRET cascade comprising at least three different types of fluorophores, arranged with (a) a plurality of initial donor fluorophores fixed in exterior positions on the structure, (b) a terminal acceptor fluorophore fixed in a central position on the structure, and (c) a plurality of intermediate fluorophores fixed in positions on the scaffold between the initial acceptor fluorophores and the terminal acceptor fluorophores.
    Type: Application
    Filed: April 14, 2015
    Publication date: October 15, 2015
    Applicant: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Susan Buckhout-White, Mario Ancona, Ellen R. Goldman, Igor L. Medintz, Joseph S. Melinger
  • Patent number: 9157842
    Abstract: A molecular concentrator comprising a thermal ratchet for driving molecules from one place to another. A plurality of linear, two-dimensional, and/or three-dimensional arrangements of heater structures are arranged on or suspended above a substrate. Each of the heater structures is configured to strongly sorb a vapor of interest when the heater structure is at room temperature and to rapidly desorb the vapor when the heater structure is at an elevated temperature. The vapor sorption of the individual heater structures is made selective by surface treatments, by monomolecular film depositions or by thicker absorbent polymer depositions. By selectively heating and cooling the heater structures, vapor molecules incident on the heater structures can be directed in a desired manner, e.g., from heater structures closest to a vapor-containing environment to a sensor.
    Type: Grant
    Filed: February 5, 2015
    Date of Patent: October 13, 2015
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Mario Ancona, Arthur W. Snow, F. Keith Perkins
  • Patent number: 8986615
    Abstract: A molecular concentrator comprising a thermal ratchet for driving molecules from one place to another. A plurality of conducting wires are arranged on or suspended above a substrate. Each of the wires is configured to strongly sorb a vapor of interest when the wire is at room temperature and to rapidly desorb the vapor when the wire is at an elevated temperature. By selectively heating and cooling the wires, vapor molecules incident on the wires can be directed in a desired manner, e.g., from the wires closest to the vapor-containing environment to a sensor.
    Type: Grant
    Filed: May 16, 2014
    Date of Patent: March 24, 2015
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Mario Ancona, Arthur W. Snow, F. Keith Perkins
  • Patent number: 8723218
    Abstract: Silicon carbide PiN diodes are presented with reduced temperature coefficient crossover points by limited p type contact area to limit hole injection in the n type drift layer in order to provide a lower current at which the diode shifts from negative temperature coefficient to a positive temperature coefficient of forward voltage for mitigating thermal runaway.
    Type: Grant
    Filed: September 6, 2012
    Date of Patent: May 13, 2014
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Karl D. Hobart, Francis J. Kub, Mario Ancona, Eugene A. Imhoff
  • Patent number: 8652959
    Abstract: A complementary metal oxide semiconductor (CMOS) device in which a single InxGa1-xSb quantum well serves as both an n-channel and a p-channel in the same device and a method for making the same. The InxGa1-xSb layer is part of a heterostructure that includes a Te-delta doped AlyGa1-ySb layer above the InxGa1-xSb layer on a portion of the structure. The portion of the structure without the Te-delta doped AlyGa1-ySb barrier layer can be fabricated into a p-FET by the use of appropriate source, gate, and drain terminals, and the portion of the structure retaining the Te-delta doped AlyGa1-ySb layer can be fabricated into an n-FET so that the structure forms a CMOS device, wherein the single InxGa1-xSb quantum well serves as the transport channel for both the n-FET portion and the p-FET portion of the heterostructure.
    Type: Grant
    Filed: February 1, 2013
    Date of Patent: February 18, 2014
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Brian R. Bennett, John Bradley Boos, Mario Ancona, James G. Champlain, Nicolas A. Papanicolaou
  • Publication number: 20130240905
    Abstract: Silicon carbide PiN diodes are presented with reduced temperature coefficient crossover points by limited p type contact area to limit hole injection in the n type drift layer in order to provide a lower current at which the diode shifts from negative temperature coefficient to a positive temperature coefficient of forward voltage for mitigating thermal runaway.
    Type: Application
    Filed: September 6, 2012
    Publication date: September 19, 2013
    Inventors: Karl D. Hobart, Francis J. Kub, Mario Ancona, Eugene A. Imhoff
  • Publication number: 20130149845
    Abstract: A complementary metal oxide semiconductor (CMOS) device in which a single InxGa1-xSb quantum well serves as both an n-channel and a p-channel in the same device and a method for making the same. The InxGa1-xSb layer is part of a heterostructure that includes a Te-delta doped AlyGa1-ySb layer above the InxGa1-xSb layer on a portion of the structure. The portion of the structure without the Te-delta doped AlyGa1-ySb barrier layer can be fabricated into a p-FET by the use of appropriate source, gate, and drain terminals, and the portion of the structure retaining the Te-delta doped AlyGa1-ySb layer can be fabricated into an n-FET so that the structure forms a CMOS device, wherein the single InxGa1-xSb quantum well serves as the transport channel for both the n-FET portion and the p-FET portion of the heterostructure.
    Type: Application
    Filed: February 1, 2013
    Publication date: June 13, 2013
    Applicant: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Brian R. Bennett, John Bradley Boos, Mario Ancona, James G. Champlain, Nicolas A. Papanicolaou
  • Patent number: 8461664
    Abstract: A complementary metal oxide semiconductor (CMOS) device in which a single InxGa1-xSb quantum well serves as both an n-channel and a p-channel in the same device and a method for making the same. The InxGa1-xSb layer is part of a heterostructure that includes a Te-delta doped AlyGa1-ySb layer above the InxGa1-xSb layer on a portion of the structure. The portion of the structure without the Te-delta doped AlyGa1-ySb barrier layer can be fabricated into a p-FET by the use of appropriate source, gate, and drain terminals, and the portion of the structure retaining the Te-delta doped AlyGa1-ySb layer can be fabricated into an n-FET so that the structure forms a CMOS device, wherein the single InxGa1-xSb quantum well serves as the transport channel for both the n-FET portion and the p-FET portion of the heterostructure.
    Type: Grant
    Filed: May 25, 2011
    Date of Patent: June 11, 2013
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Brian R. Bennett, John Bradley Boos, Mario Ancona, James G. Champlain, Nicolas A Papanicolaou