Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.

Abstract: A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.

Abstract: A method for verifying a set of policy instructions to be used by a policy decision point (PDP) in adjudicating access requests to protected resources. The policy instructions are in the form of Horn clauses or conditional tag-expressions that are validated against a known test policy or desired outcome. The policy instructions are then compiled into aggregate form. When a plurality of policy instructions creates a conflict, the policy instructions are hierarchically organized to resolve said conflict.

Abstract: An apparatus includes a fluidic coupler including an opening. A first port is in fluid communication with the opening and is to interface with an inlet of a flow cell of a sensor device. A second port is to interface with an outlet of the flow cell of the sensor device. A third port is in fluidic communication with the second port. The apparatus further includes a mechanical assembly moveable between a first position and a second position. The fluidic coupler is secured to the flow cell of the sensor device in the first position. The fluidic coupler is disengaged from the flow cell of the sensor device in the second position.

Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.

Abstract: A sensor device includes a substrate having a die attached to the substrate. The die includes an array of sensors and an array of wells cooperatively disposed over the array of sensors. The array of wells is exposed by the substrate. The sensor device further includes a flow cell secured to the substrate and defining a flow space disposed over the die and accessible to the array of wells. The flow cell defines a plurality of separate volumes. Each separate volume of the plurality of separate volumes has an inlet and an outlet. The plurality of separate volumes are separated by dividers. The dividers cover a set of wells of the array of wells.

Abstract: An apparatus for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The electric field is applied to at least one electrode having a plurality of concentric rings or concentric arcs extending radially outwards from a center point, electrically connected to a voltage source such that when voltage is applied to the at least one electrode, the concentric rings or concentric arcs alternate in voltage potential.

Abstract: An apparatus for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The electric field is applied to at least one electrode having a plurality of concentric rings or concentric arcs extending radially outwards from a center point, electrically connected to a voltage source such that when voltage is applied to the at least one electrode, the concentric rings or concentric arcs alternate in voltage potential.

Abstract: A system for controlling file access on a mobile computing device. Policy conditions are held at a policy decision point (PDP) and can be dynamically modified at run-time. Access requests to a file or set of files are intercepted by an agent that subsequently brokers the adjudication of said request via a secure, encrypted and hidden back-channel where the requestor is never allowed access to or knowledge of either the adjudication process or the parameters associated with adjudication. The PDP then returns either an access approval or denial based on said policy conditions.

Abstract: A drinking cup having a receptacle with an open end and a lid assembly configured for releasable engagement with the receptacle over the open end. A lid assembly wall has an inner surface and an inner ring extending radially inward therefrom. The inner ring defines a plurality of fluid passages. A seal assembly is configured to be retained by the inner ring, with the seal assembly being resiliently deformable between a sealed configuration and an unsealed configuration. In the sealed configuration, the upper lip sealingly engages the inner surface and fluid is prevented from flowing through a lid assembly volume defined between the lid assembly and the seal assembly. In the open configuration fluid is permitted to flow from the receptacle, through the plurality of fluid passages, and between a gap defined by a portion of the seal lip and a corresponding portion of the sealing surface.

Abstract: A container includes a lower portion and a lid to connect with the lower portion. The lid includes a fluidic interface, a swing bar, and a counter structure disposed on an opposite side of the fluidic interface relative to the swing bar. The fluidic interface includes a first liquid port, a second liquid port, a gas port, and a seal ring disposed around the first liquid port, the second liquid port, and the gas port, the first liquid port disposed along a center axis of the container.

Abstract: A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.

Abstract: A system and methods for context-aware and situation-aware secure, policy-based access control for computing devices. The invention enhances the previously disclosed policy-based control system by adding contextual information to the set of resources by which a policy decision point can adjudicate a query to execute a transaction or to access a secure resource. Policy information points are able to store information collected over time related to resources under the control of the system. The system can further include an analytical processing engine capable of inferring new information from existing information that also can be used by the decision points. The policy information points provide context to the decision. They are also able to consider and include information that is external to the system or detected outside the system itself.

Abstract: An apparatus for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The electric field is applied to at least one electrode having a plurality of concentric rings or concentric arcs extending radially outwards from a center point, electrically connected to a voltage source such that when voltage is applied to the at least one electrode, the concentric rings or concentric arcs alternate in voltage potential.

Abstract: In an example, a method for preparing a nucleotide solution includes flowing an aqueous solution from an initial solution storage of a sequencing instrument continuously through a container fluidically coupled to the sequencing instrument, the container comprising a nucleotide concentrate; and collecting the aqueous solution with nucleotide in a storage container.

Abstract: The present invention relates to uniform nanostructure biosensors and methods of calibrating the response of nanostructure biosensors. The invention overcomes device to device variability that has made quantitative detection difficult. The described biosensors have uniform characteristics that allow for more reliable comparison across devices. The methods of the invention comprise normalizing the initial current rate, as measured by the nanostructure biosensor following the addition of an analyte, to device characteristics of the biosensor. The device characteristics of the biosensor which can be used to normalize the response include baseline current and transconductance, Calibration of responses allows for the generation of calibration curves for use in all devices to quantitatively detect an analyte, without the need for individual device calibration.

Abstract: A fan containment system for fitment around an array of radially extending fan blades mounted on a hub in an axial gas turbine engine. The fan containment system comprises a fan case having an annular casing element for encircling an array of fan blades and a hook projecting in a generally radially inward direction from the annular casing element. An annular fan track liner is provided comprising a first fan track liner panel positioned circumferentially adjacent a second fan track liner panel. A coupling connects the first fan track liner panel to the second fan track liner panel and the first fan track liner panel and the second fan track liner panel each comprise a groove along an axial face thereof and a portion of the coupling is received in each of said grooves.

Abstract: An elbow prosthesis can include a humeral component having a yoke, an ulnar component having a head, a humeral bearing positionable in a base of the humeral component, an ulnar bearing assembly configured to engage with the head, and a pin extendable through the bearing assembly and the head. The pin can be extendable into first and second ears of the yoke to enable pivotable movement of the ulnar component relative to the humeral component. The elbow prosthesis can include a first fastener insertable through the first ear of the yoke and configured to engage with the pin, and a second fastener insertable through the second ear of the yoke and configured to engage with the pin.

Abstract: Systems and methods for secure, policy-based, access control and management of mobile computing devices, including policy decision enforcement mechanisms, device and private network presence testing, aspects of file system controls, policy set sanity checking algorithms, performance optimizations.

Abstract: A drinking cup having a receptacle with an open end and a lid assembly configured for releasable engagement with the receptacle over the open end. A lid assembly wall has an inner surface and an inner ring extending radially inward therefrom. The inner ring defines a plurality of fluid passages. A seal assembly is configured to be retained by the inner ring, with the seal assembly being resiliently deformable between a sealed configuration and an unsealed configuration. In the sealed configuration, the upper lip sealingly engages the inner surface and fluid is prevented from flowing through a lid assembly volume defined between the lid assembly and the seal assembly. In the open configuration fluid is permitted to flow from the receptacle, through the plurality of fluid passages, and between a gap defined by a portion of the seal lip and a corresponding portion of the sealing surface.