Abstract: The present disclosure relates to an improved methodology for phenotyping and molecular characterisation of single cells using high-throughput and multiplexed targeted long-read single cell sequencing. In one particular example, the present disclosure relates to a methodology which combines targeted long-read sequencing with short-read based transcriptome profiling of barcoded single cell libraries generated by droplet-based partitioning for high throughput deep single cell profiling.

Abstract: The invention relates to compositions, methods, devices, systems, and kits for detecting and characterizing molecules using a nanopore device.

Abstract: A system comprises a first workstation that locally instantiates a design tool configured to allow a designer to specify a design for a web page. A data store is locally available to the first workstation, and receives and stores the design as a saved design. A second workstation provides access to a code editor to allow a programmer to specify a modification to an encoding of the design. A server means instantiates the code editor for the second workstation, saves the modification as a saved modification, and serves the encoding of the design in accordance with the modification by combining the saved modification with the saved design. The server means seamlessly combines the saved design and the saved modification such that the saved modification and the saved design can each be independently altered and recombined to produce a modified encoding of the design.

Abstract: A method for generating and using multi-view masters involves selecting a master in a design environment. A widget is added to the master. A first view is selected for the master. A first widget characterization of the widget is received. The first widget characterization is associated with the first view of the master. A second view of the master is selected. A second widget characterization of the widget is received. The second widget characterization is associated with the second view of the master. An instance of the master is placed in a containing context. A first instance view selection is received, the first instance view selecting the first view of the master for the first instance of the master. The first instance of the master is displayed within the containing context, the widget being displayed in accordance with the first widget characterization associated with the first view of the master.

Abstract: Various methods and systems for documenting interactive graphical design include an exemplary graphical design environment stored on a non-transitory computer-readable medium that comprises a documentation element in an interactive graphical design. The design environment also comprises a design element that displays a plurality of states in a rendering of the interactive graphical design in an external player. The documentation element: (i) is enabled to receive a selection from the user of a state in the plurality of states; and (ii) displays a representation of the design element in the state in response to receiving the selection from the user. The documentation element and design element are both instantiated by a processor operating in combination with the non-transitory computer-readable medium.

Abstract: A focused specification for an interactive graphical design is generated by receiving a first user input from a user, the first user input identifying a first design element of the interactive graphical design. A set of selectable data elements associated with the first design element is identified and a specification template that includes the set of selectable data elements is generated. A second user input is received from the user, the second user input identifying a set of selected data elements using the generated specification template. The set of selected data elements includes one or more of the selectable data elements. The focused specification is generated using the set of selected data elements, the focused specification including the set of selected data elements and excluding selectable data elements that were not selected.

Abstract: Computer-implemented methods for allowing users to specify interactive graphical designs are provided. The graphical designs can comprise multiple dimension versions—such as a tablet dimension version or a phone dimension version. Some of the methods involve an inheritance structure that defines a first dimension version of the design as a child of a second dimension version of the design. Specifications for properties of widgets in the graphical design are applied to the design in accordance with the inheritance specification. Some of the methods involve an inheritance characterization that determines how properties of a widget are affected by the inheritance structure across different dimension versions. Some of the methods involve an existence property for the widgets.

Abstract: Computer-implemented methods for allowing users to specify interactive graphical designs are provided. The graphical designs can comprise multiple dimension versions—such as a tablet dimension version or a phone dimension version. Some of the methods involve an inheritance structure that defines a first dimension version of the design as a child of a second dimension version of the design. Specifications for properties of widgets in the graphical design are applied to the design in accordance with the inheritance specification. Some of the methods involve an inheritance characterization that determines how properties of a widget are affected by the inheritance structure across different dimension versions. Some of the methods involve an existence property for the widgets.

Abstract: An apparatus and method for generating a damaged vehicle estimate for a damaged vehicle. A mobile device is used to capture a photo image of the damaged vehicle and receive vehicle information from a user. The mobile device displays a graphical image of the damaged vehicle. A user can provide input on the graphical image that indicates a location of damage on the damaged vehicle. The photo image, marked graphical image and the vehicle information are transferred from the mobile device to a computer where a damaged vehicle estimate is generated. The damaged vehicle estimate is transferred to the mobile device and displayed to a user.

Abstract: A method for generating and using multi-view masters involves selecting a master in a design environment. A widget is added to the master. A first view is selected for the master. A first widget characterization of the widget is received. The first widget characterization is associated with the first view of the master. A second view of the master is selected. A second widget characterization of the widget is received. The second widget characterization is associated with the second view of the master. An instance of the master is placed in a containing context. A first instance view selection is received, the first instance view selecting the first view of the master for the first instance of the master. The first instance of the master is displayed within the containing context, the widget being displayed in accordance with the first widget characterization associated with the first view of the master.

Abstract: A sectional radiator seal arrangement including a sectional radiator having a core and a bonnet, a nozzle defined by a cylindrical sidewall extending from and in fluid communication with the bonnet and the core, the nozzle configured for creating a seal with a radiator tank and a sleeve formed from a corrosion resistant material fitted about a portion of the cylindrical sidewall of the nozzle. A retaining compound can be provided between the sleeve and the radiator tank for preventing an ingress of coolant at the seal and into contact with either the nozzle or the bonnet. A method of reducing corrosion of the radiator seal also is provided.

Abstract: This disclosure relates to methods and systems for processing of sequencing data streams. The system receives sequences from a sequencer and stores them as data records on a database. The sequences are associated with a counter indicative of a number of times the associated sequence has been sequenced. The system progressively receives a further sequence as streaming data from the sequence. While receiving the further sequence, the system matches the streaming data against the stored sequences to determine a matching score. Upon the matching score exceeding a matching threshold for one of the multiple sequences in the database, the system selects the one of the sequences in the database based on the matching score and stores the further sequence on non-volatile memory where the counter value associated with the selected sequence is below a saturation threshold. The system also terminates the receiving where the counter value is above the saturation threshold.

Abstract: A method for generating and using multi-view masters involves selecting a master in a design environment. A widget is added to the master. A first view is selected for the master. A first widget characterization of the widget is received. The first widget characterization is associated with the first view of the master. A second view of the master is selected. A second widget characterization of the widget is received. The second widget characterization is associated with the second view of the master. An instance of the master is placed in a containing context. A first instance view selection is received, the first instance view selecting the first view of the master for the first instance of the master. The first instance of the master is displayed within the containing context, the widget being displayed in accordance with the first widget characterization associated with the first view of the master.

Abstract: According to some embodiments, system and methods are provided, comprising at least one asset; a computer programmed with a data share module for the asset, the data share module for controlling data flow in the asset; the computer including a data share processor and a memory in communication with the data share processor, the memory storing the data share module and additional program instructions, wherein the data share processor is operative with the data share module and additional program instructions to perform functions as follows: receiving a message from a source at the data share module; determining, via the data share module, whether the source is one of a non-real time domain of the asset and the real-time domain of the asset; determining, via the data share module, when a destination is able to respond to the message, wherein the destination is one of the non-real time domain and the real-time domain, and wherein the destination is different from the source; transmitting, via the data share modu

Abstract: A method for generating and using multi-view masters involves selecting a master in a design environment. A widget is added to the master. A first view is selected for the master. A first widget characterization of the widget is received. The first widget characterization is associated with the first view of the master. A second view of the master is selected. A second widget characterization of the widget is received. The second widget characterization is associated with the second view of the master. An instance of the master is placed in a containing context. A first instance view selection is received, the first instance view selecting the first view of the master for the first instance of the master. The first instance of the master is displayed within the containing context, the widget being displayed in accordance with the first widget characterization associated with the first view of the master.

Abstract: A method is provided for making footwear utilizing a spray-on material. The method can include spraying a spray-on fabric onto surfaces of a three dimensional last. The last can be produced using additive manufacturing techniques, such as 3D printing, based on data corresponding to dimensions and contours of a consumer's foot, and/or preselected aesthetic footwear designs. The spray-on fabric is sprayed onto the surfaces of the last and allowed to cure to form a flexible, non-woven fabric upper. The upper can be removed from the last and turned inside out. With the upper turned inside out, a sole can be joined to the fabric upper to form the footwear.

Abstract: The present invention provides a sensor system for measuring a parameter (e.g. volume, temperature or pressure) of a target, the system comprising a diaphragm, a sensor for measuring the axial spacing between the sensor and the diaphragm, and an axially adjustable mount. The mount has a first axial end for mounting the diaphragm which is axially movable relative to the sensor and an opposing, second axial end which is axially fixed relative to the sensor. The diaphragm and mount define a chamber for receiving the target or for being received within the target. In use, the axial spacing between the first axial end and the second axial end of the mount and thus the axial spacing between the diaphragm and sensor varies as a result of a change in the parameter differential across the diaphragm.

Abstract: A system comprises a first workstation that locally instantiates a design tool configured to allow a designer to specify a design for a web page. A data store is locally available to the first workstation, and receives and stores the design as a saved design. A second workstation provides access to a code editor to allow a programmer to specify a modification to an encoding of the design. A server means instantiates the code editor for the second workstation, saves the modification as a saved modification, and serves the encoding of the design in accordance with the modification by combining the saved modification with the saved design. The server means seamlessly combines the saved design and the saved modification such that the saved modification and the saved design can each be independently altered and recombined to produce a modified encoding of the design.

Abstract: Various methods and systems for documenting interactive graphical design are provided. An exemplary graphical design environment stored on a non-transitory computer-readable medium comprises a documentation element in an interactive graphical design. The design environment also comprises a design element that displays a plurality of states in a rendering of the interactive graphical design in an external player. The documentation element: (i) is enabled to receive a selection from the user of a state in the plurality of states; and (ii) displays a representation of the design element in the state in response to receiving the selection from the user. The documentation element and design element are both instantiated by a processor operating in combination with the non-transitory computer-readable medium.

Abstract: The Multi-dimensional Situational Awareness and Risk Mitigation Apparatuses, Methods and Systems (“MdSARM”) transforms survey, situational atmospheric (e.g., gps, camera, audio, time, etc.) inputs via MdSARM components into situational risk awareness notices, situational prevention warnings, augmented reality situational risk awareness overlays outputs. MdSARM includes a situational awareness risk assessment and mitigation apparatus, comprising a memory, a component collection in the memory, including, a processor disposed in communication with the memory, and configured to issue a plurality of processing instructions from the component collection stored in the memory. Instructions include to obtain a survey datastructure to quantify the component variables of situational awareness, and correlate survey result data multi-variate vector to known risk matrix database.