Patents by Inventor Masahiko Hagihara

Masahiko Hagihara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11932841
    Abstract: The present invention provides a cell cultivation module comprising a polymer porous film and a casing that has two or more culture medium inflow/outflow ports and accommodates the polymer porous film, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, and the polymer porous film is accommodated within the casing.
    Type: Grant
    Filed: July 25, 2017
    Date of Patent: March 19, 2024
    Assignee: UBE CORPORATION
    Inventors: Masahiko Hagihara, Shinsaku Fuse, Motohisa Shimizu, Yukinori Wada
  • Publication number: 20220340878
    Abstract: The present invention relates to a cell preparation method that includes a step in which cells are applied to a polyimide porous film and cultivated, wherein the polyimide porous film is a polyimide porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, and the polyimide porous film is produced by a method including the following steps: (1) a step in which a poly(amic acid) solution comprising poly(amic acid) and an organic polar solvent is flow cast in a film shape and the result is immersed in or brought into contact with a coagulation medium to create a porous film of poly(amic acid); and (2) a step in which the porous film of poly(amic acid) obtained in step (1) is heat-treated and imidized.
    Type: Application
    Filed: June 27, 2022
    Publication date: October 27, 2022
    Inventors: Masahiko HAGIHARA, Shyusei OHYA
  • Publication number: 20220290082
    Abstract: The present invention provides a method for producing an exosome, comprising a step of applying a cell to a cell culture module to culture the cell and a step of producing an exosome by the cell, wherein the cell culture module is provided with a polymer porous film and a casing having at least two culture medium in-flow/out-flow ports and having the polymer porous film placed therein.
    Type: Application
    Filed: August 11, 2020
    Publication date: September 15, 2022
    Inventors: Masahiko HAGIHARA, Takashi HARADA, Shotaro YAMADA, Akira TAKAMA
  • Publication number: 20220290083
    Abstract: The purpose of the present invention is to provide a cell culturing method using a small-piece polymer porous membrane. The present invention provides a cell culturing method which does not require stirring and comprises applying cells to a small-piece polymer porous membrane having an area of a surface layer A or B of at most 4 mm2, and culturing the cells. The small-piece polymer porous membrane has a characteristic three-dimensional structure, and a medium can be more easily supplied and recovered by utilizing the property that small-piece polymer porous membranes are dispersed in a cell culture solution and/or small-piece polymer porous membranes are accumulated in multiple layers, and thus the small-piece polymer porous membranes are dispersed and/or laminated on a bottom part of a cell culture vessel. Consequently, long-term culture and mass culture are achieved, and thus the stable long-term production of exosomes is possible.
    Type: Application
    Filed: August 11, 2020
    Publication date: September 15, 2022
    Inventors: Masahiko HAGIHARA, Motonobu HIDAKA, Takashi HARADA, Hayato KONOSHIMA
  • Patent number: 10982186
    Abstract: The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film.
    Type: Grant
    Filed: January 25, 2018
    Date of Patent: April 20, 2021
    Assignee: UBE INDUSTRIES, LTD.
    Inventors: Masahiko Hagihara, Motohisa Shimizu, Yukinori Wada
  • Publication number: 20210047595
    Abstract: The present invention provides a cell culture device characterized by comprising: a culture container; a rotating polymeric porous film housing part which is housed in the culture container, and has one or more culture medium inlet/outlet openings; and a polymeric porous film which is housed in the rotating polymeric porous film housing part, wherein the polymeric porous film is a modular polymeric porous film, and the rotating polymeric porous film housing part is rotated independently of the culture container.
    Type: Application
    Filed: January 24, 2019
    Publication date: February 18, 2021
    Inventors: Masahiko HAGIHARA, Takashi HARADA, Akihiro MATSUBAYASHI, Shinsaku FUSE
  • Publication number: 20210040430
    Abstract: Provided is a means that applies uniform culture conditions to a plurality of porous polymer membranes and makes it possible to stably culture a large quantity of cells. A cell culture module that comprises: a top part; a bottom part; a side part that has a culture solution in/outflow port; a plurality of partition parts that partition a space that is formed by the top part, the bottom part, and the side part and have a culture solution in/outflow port; and porous polymer membranes that are respectively fixed in at least two interstitial spaces selected from the interstitial space between the top part and the adjacent partition part, the interstitial space between the bottom part and the adjacent partition part, and the plurality of interstitial spaces between adjacent partition parts.
    Type: Application
    Filed: January 24, 2019
    Publication date: February 11, 2021
    Inventors: Masahiko HAGIHARA, Takashi HARADA, Akihiro MATSUBAYASHI, Shinsaku FUSE
  • Patent number: 10738277
    Abstract: The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: August 11, 2020
    Assignee: UBE INDUSTRIES, LTD.
    Inventors: Masahiko Hagihara, Motohisa Shimizu, Yukinori Wada
  • Patent number: 10696943
    Abstract: The present invention pertains to a method for the mass cultivation of cells, and a cell cultivation device and kit. The present invention further pertains to a continuous cell cultivation method and a continuous cell cultivation device in which a carrier is used.
    Type: Grant
    Filed: January 26, 2018
    Date of Patent: June 30, 2020
    Assignee: UBE INDUSTRIES, LTD.
    Inventors: Masahiko Hagihara, Motohisa Shimizu, Yukinori Wada
  • Patent number: 10647956
    Abstract: The purpose of the present invention is to provide a method for isolating, removing and analyzing cells. Provided is a method for isolating, removing and analyzing cells by filtering a liquid specimen containing cells through a porous polyimide film, and subjecting the cells captured by the film which did not pass through the porous polyimide film, or the cells in the liquid specimen which did pass through the porous polyimide film, to an examination of one or more cell properties selected from a group consisting of cell number or type, internal or external cell structure, type or amount of cell surface antigen, type or amount of material secreted from cells, cell adhesion, and cell survival rate.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: May 12, 2020
    Assignees: UBE INDUSTRIES, LTD., UNIVERSITY OF TSUKUBA
    Inventors: Akiyoshi Fukamizu, Jun-Dal Kim, Masahiko Hagihara, Yukinori Wada
  • Patent number: 10626365
    Abstract: The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: April 21, 2020
    Assignee: UBE INDUSTRIES, LTD.
    Inventors: Masahiko Hagihara, Motohisa Shimizu, Yukinori Wada
  • Patent number: 10590388
    Abstract: The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: March 17, 2020
    Assignees: UBE INDUSTRIES, LTD., KURUME UNIVERSITY
    Inventors: Keisuke Ohta, Shingo Hirashima, Masahiko Hagihara, Motohisa Shimizu
  • Publication number: 20200071665
    Abstract: The present invention relates to: a method for inhibiting the differentiation of a neural stem cell by culturing the neural stem cell on a polymer porous membrane; a method for preparing a neural stem cell; and a method for differentiating and inducing a neural stem cell.
    Type: Application
    Filed: March 22, 2018
    Publication date: March 5, 2020
    Inventors: Yoshitoshi KASUYA, Kento YOSHIOKA, Masahiko HAGIHARA
  • Patent number: 10519112
    Abstract: The present invention provides: a method for producing a mixed sulfonic acid anhydride of the general formula (1) by reacting a sulfonic acid compound of the general formula (2) with a perfluoroalkyl sulfonic acid anhydride or a perfluoroalkyl sulfonic acid halide; and a method for producing a sulfonamide compound of the general formula (4) obtained by reacting an amine compound of the general formula (3) with a mixed sulfonic acid anhydride of the general formula (1).
    Type: Grant
    Filed: June 10, 2015
    Date of Patent: December 31, 2019
    Assignee: UBE INDUSTRIES, LTD.
    Inventors: Yasuhito Yamamoto, Masayoshi Oue, Masahiko Hagihara, Yukinori Wada, Gen Mizuno, Yasunori Tsuzaki, Kenichi Arai
  • Patent number: 10519478
    Abstract: The present invention relates to a method of using cells to produce a substance, and involves applying the cells to a polyimide porous film, culturing the cells and producing the substance by means of the cells.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: December 31, 2019
    Assignee: UBE INDUSTRIES, LTD.
    Inventors: Masahiko Hagihara, Motohisa Shimizu, Yukinori Wada
  • Patent number: 10508264
    Abstract: The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: December 17, 2019
    Assignees: UBE INDUSTRIES, LTD., KURUME UNIVERSITY
    Inventors: Keisuke Ohta, Shingo Hirashima, Masahiko Hagihara, Motohisa Shimizu
  • Patent number: 10479974
    Abstract: The present invention pertains to a method for the mass cultivation of cells, and a cell cultivation device and kit. The present invention further pertains to a continuous cell cultivation method and a continuous cell cultivation device in which a carrier is used.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: November 19, 2019
    Assignee: UBE INDUSTRIES, LTD.
    Inventors: Masahiko Hagihara, Motohisa Shimizu, Yukinori Wada
  • Publication number: 20190330581
    Abstract: The present invention provides a cell cultivation module comprising a polymer porous film and a casing that has two or more culture medium inflow/outflow ports and accommodates the polymer porous film, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, and the polymer porous film is accommodated within the casing.
    Type: Application
    Filed: July 25, 2017
    Publication date: October 31, 2019
    Inventors: Masahiko HAGIHARA, Shinsaku FUSE, Motohisa SHIMIZU, Yukinori WADA
  • Patent number: 10443037
    Abstract: The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film.
    Type: Grant
    Filed: January 25, 2018
    Date of Patent: October 15, 2019
    Assignee: UBE INDUSTRIES, LTD.
    Inventors: Masahiko Hagihara, Motohisa Shimizu, Yukinori Wada
  • Publication number: 20190284525
    Abstract: The present invention relates to a cell preparation method that includes applying cells to a polyether sulfone porous film and cultivating the cells.
    Type: Application
    Filed: July 25, 2017
    Publication date: September 19, 2019
    Inventors: Masahiko HAGIHARA, Shusei OHYA