Abstract: The present invention provides a cell cultivation module comprising a polymer porous film and a casing that has two or more culture medium inflow/outflow ports and accommodates the polymer porous film, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, and the polymer porous film is accommodated within the casing.

Abstract: The present invention relates to a cell preparation method that includes a step in which cells are applied to a polyimide porous film and cultivated, wherein the polyimide porous film is a polyimide porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, and the polyimide porous film is produced by a method including the following steps: (1) a step in which a poly(amic acid) solution comprising poly(amic acid) and an organic polar solvent is flow cast in a film shape and the result is immersed in or brought into contact with a coagulation medium to create a porous film of poly(amic acid); and (2) a step in which the porous film of poly(amic acid) obtained in step (1) is heat-treated and imidized.

Abstract: The present invention provides a method for producing an exosome, comprising a step of applying a cell to a cell culture module to culture the cell and a step of producing an exosome by the cell, wherein the cell culture module is provided with a polymer porous film and a casing having at least two culture medium in-flow/out-flow ports and having the polymer porous film placed therein.

Abstract: The purpose of the present invention is to provide a cell culturing method using a small-piece polymer porous membrane. The present invention provides a cell culturing method which does not require stirring and comprises applying cells to a small-piece polymer porous membrane having an area of a surface layer A or B of at most 4 mm2, and culturing the cells. The small-piece polymer porous membrane has a characteristic three-dimensional structure, and a medium can be more easily supplied and recovered by utilizing the property that small-piece polymer porous membranes are dispersed in a cell culture solution and/or small-piece polymer porous membranes are accumulated in multiple layers, and thus the small-piece polymer porous membranes are dispersed and/or laminated on a bottom part of a cell culture vessel. Consequently, long-term culture and mass culture are achieved, and thus the stable long-term production of exosomes is possible.

Abstract: The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film.

Abstract: The present invention provides a cell culture device characterized by comprising: a culture container; a rotating polymeric porous film housing part which is housed in the culture container, and has one or more culture medium inlet/outlet openings; and a polymeric porous film which is housed in the rotating polymeric porous film housing part, wherein the polymeric porous film is a modular polymeric porous film, and the rotating polymeric porous film housing part is rotated independently of the culture container.

Abstract: Provided is a means that applies uniform culture conditions to a plurality of porous polymer membranes and makes it possible to stably culture a large quantity of cells. A cell culture module that comprises: a top part; a bottom part; a side part that has a culture solution in/outflow port; a plurality of partition parts that partition a space that is formed by the top part, the bottom part, and the side part and have a culture solution in/outflow port; and porous polymer membranes that are respectively fixed in at least two interstitial spaces selected from the interstitial space between the top part and the adjacent partition part, the interstitial space between the bottom part and the adjacent partition part, and the plurality of interstitial spaces between adjacent partition parts.

Abstract: The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film.

Abstract: The present invention pertains to a method for the mass cultivation of cells, and a cell cultivation device and kit. The present invention further pertains to a continuous cell cultivation method and a continuous cell cultivation device in which a carrier is used.

Abstract: The purpose of the present invention is to provide a method for isolating, removing and analyzing cells. Provided is a method for isolating, removing and analyzing cells by filtering a liquid specimen containing cells through a porous polyimide film, and subjecting the cells captured by the film which did not pass through the porous polyimide film, or the cells in the liquid specimen which did pass through the porous polyimide film, to an examination of one or more cell properties selected from a group consisting of cell number or type, internal or external cell structure, type or amount of cell surface antigen, type or amount of material secreted from cells, cell adhesion, and cell survival rate.

Abstract: The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film.

Abstract: The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site.

Abstract: The present invention relates to: a method for inhibiting the differentiation of a neural stem cell by culturing the neural stem cell on a polymer porous membrane; a method for preparing a neural stem cell; and a method for differentiating and inducing a neural stem cell.

Abstract: The present invention relates to a method of using cells to produce a substance, and involves applying the cells to a polyimide porous film, culturing the cells and producing the substance by means of the cells.

Abstract: The present invention provides: a method for producing a mixed sulfonic acid anhydride of the general formula (1) by reacting a sulfonic acid compound of the general formula (2) with a perfluoroalkyl sulfonic acid anhydride or a perfluoroalkyl sulfonic acid halide; and a method for producing a sulfonamide compound of the general formula (4) obtained by reacting an amine compound of the general formula (3) with a mixed sulfonic acid anhydride of the general formula (1).

Abstract: The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site.

Abstract: The present invention pertains to a method for the mass cultivation of cells, and a cell cultivation device and kit. The present invention further pertains to a continuous cell cultivation method and a continuous cell cultivation device in which a carrier is used.

Abstract: The present invention provides a cell cultivation module comprising a polymer porous film and a casing that has two or more culture medium inflow/outflow ports and accommodates the polymer porous film, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, and the polymer porous film is accommodated within the casing.

Abstract: The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film.

Abstract: The present invention relates to a cell preparation method that includes applying cells to a polyether sulfone porous film and cultivating the cells.