Abstract: The present invention provides a cell cultivation device that is characterized by comprising: a polymer porous film; a cell cultivation part that has the polymer porous film; a shaft that passes through the cell cultivation part; and a cultivation tank in which at least one portion of the cell cultivation part is immersed, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in communication with the macrovoids, the cel

Abstract: The present invention relates to a method to suppress stem cell differentiation, said method including: (1) a step in which the stem cells are applied to a polymer porous film; and (2) a step in which the stem cells are cultivated and allowed to multiply, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, and the holes in the surface layers A and B are in communication with the macrovoids.

Abstract: The present invention relates to a method to suppress the dedifferentiation of cells that readily dedifferentiate, said method including: (1) a step in which the cells are applied to a polymer porous film; and (2) a step in which the cells are cultivated and allowed to multiply, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, and the holes in the surface layers A and B are in communication with the macrovoids.

Abstract: Provided are a cell cultivation device comprising a polymer porous film, and a cell cultivation method using said cell cultivation device. The cell cultivation device comprises: two or more levels of parallel or antiparallel flat flow paths; a polymer porous film that is positioned in the flat flow paths; a culture medium supply means that is positioned at one end of the flat flow paths; a culture medium discharge means that is positioned at the other end of the flat flow paths; a head tank that is positioned at an upper section of the flat flow path of the uppermost level; a culture medium distribution means that distributes a culture broth from the head tank to the flat flow paths; and a culture medium recovery means that recovers the culture broth that is discharged from the culture medium discharge means.

Abstract: The present invention pertains to an implant for treatment of a bone lesion site, the implant including a polymer porous film and a layer containing a biocompatible material, wherein the polymer porous film is a polymer porous membrane of a three-layer structure having a porous surface layer A, a porous surface layer B, and a macrovoid layer interposed between the surface layer A and the surface layer B; the average pore diameter of the pores in surface layer A is smaller than the average pore diameter of the pores in surface layer B; the macrovoid layer has a partition joined to the surface layers A and B and a plurality of macrovoids enclosed by the partition and the surface layers A and B; and the pores in the surface layers A and B communicate with the macrovoids.

Abstract: The present invention provides a cell cultivation device comprising a polymer porous film, and a cell cultivation method using said cell cultivation device. Provided is a cell cultivation device that has a siphon structure or a shishi-odoshi structure, said cell cultivation device comprising: a polymer porous film; a cell cultivation part that accommodates the polymer porous film; a liquid reservoir part that has the cell cultivation part stored in an interior thereof; a culture medium supply means that is positioned at an upper section of the liquid reservoir part; a culture medium discharge means that is positioned at a lower section of the liquid reservoir part; and a culture medium recovery means that recovers the culture medium that is collected in the liquid reservoir part and is intermittently expelled.

Abstract: The present invention provides a cell cultivation method that includes: (1) a step in which a first culture medium including suspended cells is applied to a cell cultivation module; (2) a step in which a temperature at which cell cultivation is possible is maintained, and the cells are made to adsorb to the cell cultivation module; and (3) a step in which the cell cultivation module to which the cells are adsorbed is cultivated within a culture vessel with a second culture medium, wherein a polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that

Abstract: The present invention relates to a cell preparation method that includes a step in which cells are applied to a polyimide porous film and cultivated, wherein the polyimide porous film is a polyimide porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, and the polyimide porous film is produced by a method including the following steps: (1) a step in which a poly(amic acid) solution comprising poly(amic acid) and an organic polar solvent is flow cast in a film shape and the result is immersed in or brought into contact with a coagulation medium to create a porous film of poly(amic acid); and (2) a step in which the porous film of poly(amic acid) obtained in step (1) is heat-treated and imidized.

Abstract: The present invention provides a cell cultivation device comprising: a polymer porous film; a cell cultivation part that accommodates the polymer porous film; a culture medium supply means that is positioned at an upper section of the cell cultivation part; and a culture medium recovery means that is positioned at a lower section of the cell cultivation part, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, the holes in the surface layers A and B are in commu

Abstract: The purpose of the present invention is to provide a highly safe and highly effective pharmaceutical composition and the like for the prevention and treatment of respiratory diseases. The present invention provides a pharmaceutical composition with which it is possible to effectively repair damaged lung tissue by including a cell culture supernatant fluid derived from lung tissue. The present invention also provides a method and a kit for using said culture supernatant fluid to induce differentiation into lung surfactant protein positive cells.

Abstract: The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site.

Abstract: The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film.

Abstract: The present invention pertains to a method for the mass cultivation of cells, and a cell cultivation device and kit. The present invention further pertains to a continuous cell cultivation method and a continuous cell cultivation device in which a carrier is used.

Abstract: The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film.

Abstract: The present invention relates to a method for culturing bone marrow cells, in which bone marrow cells are applied to a porous polyimide film and cultured. Moreover, the present invention relates to a porous polyimide film for healing a bone injury site.

Abstract: The purpose of the present invention is to provide a method for isolating, removing and analyzing cells. Provided is a method for isolating, removing and analyzing cells by filtering a liquid specimen containing cells through a porous polyimide film, and subjecting the cells captured by the film which did not pass through the porous polyimide film, or the cells in the liquid specimen which did pass through the porous polyimide film, to an examination of one or more cell properties selected from a group consisting of cell number or type, internal or external cell structure, type or amount of cell surface antigen, type or amount of material secreted from cells, cell adhesion, and cell survival rate.

Abstract: The present invention pertains to a method for the mass cultivation of cells, and a cell cultivation device and kit. The present invention further pertains to a continuous cell cultivation method and a continuous cell cultivation device in which a carrier is used.

Abstract: The present invention relates to a method of using cells to produce a substance, and involves applying the cells to a polyimide porous film, culturing the cells and producing the substance by means of the cells.

Abstract: The present invention relates to a cell culturing method and cell culturing apparatus and kit for use therewith. The method includes applying cells on a porous polyimide film and culturing. One embodiment includes a process for sowing cells on the surface of the film. Another embodiment includes a process for placing a cell suspension on the dried surface of the film and leaving the film undisturbed, moving the film to promote liquid efflux, or stimulating a portion of the surface to entangle the cell suspension into the film, and then retaining the cells in suspension inside the film while allowing moisture to flow out. Another embodiment includes a process for moistening one or both surfaces of the film with a cell culture solution or sterilized liquid, loading a cell suspension on the moistened film, retaining the cells in suspension inside the film, and allowing moisture to flow out.

Abstract: The present invention relates to a cell culturing method and kit. More specifically, it relates to a cell culturing method and kit using a support that is exposed to the air. It further relates to a method of culturing cells by allowing them to migrate onto a porous polyimide film.