Abstract: When transfer a method from a measurement system of a liquid chromatograph to a measurement system under a condition of a high pressure and a high velocity, an influence due to analysis condition cannot be neglected. Further, since a transfer value obtained by calculation is a logical value, an improper result can be obtained in an actual measurement. Further, since conventional methods are optimized by such as know-how based on previous experiences, how to carry on these know-how to a new method is remained to be solved. One of the features of the present invention is to use a value of dwell volume so as to minimize a deviation of measurement result due to methods before and after transfer. Another is, when a deviation of the measurement result is caused, to calculate a correction value for correcting the deviation from analysis of measurement data and to automatically reflect the correction value for the method transfer.

Abstract: Provided is a liquid chromatograph pump system which sucks eluent from an eluent reserving container, and which feeds pressurized high pressure eluent into a column, wherein a plunger reciprocating in a cylinder is formed on its outer peripheral surface with a stepped part along the sliding direction of the plunger in the cylinder so as to define a working chamber between the stepper part and the inner wall surface of the cylinder, the flow rate of the liquid chromatograph pump being determined by the volume of the working chamber and the traveling speed of the plunger, thereby it is possible to stably feed a liquid at an extremely low flow rate, and to satisfactorily expel air bubbles upon a start of the liquid chromatograph pump system.

Abstract: The present invention provides a separation column including a monolithic rod without a resin coating material. The separation column can prevent the monolithic rod from destroying, and a liquid can be delivered at a high pressure into the separation column. The separation column includes the monolithic rod (monolithic silica rod) in a tapered-columnar shape, and a clad with the interior formed in a tapered-columnar shape. The monolithic rod is built into the clad.

Abstract: There is provided a user interface which enables all users to input a purpose of use of a chromatography data system, recognize a series of operation flow of the analysis of chromatograph data according to the input purpose, improve an operability and prevent an operation error regardless of the user's knowledge on analyzing the chromatograph data or the user's proficiency in the chromatography data system. In a chromatograph data processing, the present invention relates to recognizing chromatograph data analyzing means on a picture and displaying the chromatograph data analyzing means on the picture in accordance with purpose of use. According to the present invention, the chromatography data system can be easily operated regardless of the knowledge on the analysis of chromatograph data or proficiency in the chromatography data system.

Abstract: An object of the present invention is to improve the accuracy in analysis repeatability of a liquid chromatography apparatus that feeds different eluent by use of a plurality of pumps and achieves the mixture of eluent. Each of the pumps includes means for notifying an automatic sampler and a higher-level control unit that the specified timing of a liquid feeding cycle is reached. A pump whose liquid feeding cycle is the slowest transmits own information so that analysis is synchronized with a liquid feeding cycle. Moreover, a cycle position of a liquid feeding cycle at the end of analysis is expected, and the analysis time is automatically adjusted so that the wait time until the start of the next analysis becomes the shortest.

Abstract: A method for analyzing a plurality of amino acids in a fluid sample by a user is provided comprising the steps of introducing the sample into a buffer solution, passing the sample in the buffer solution through a separation column and setting a lithium ion concentration in the buffer to no more than 0.3 mols/L up to a time before ?-aminoisobutyric acid (?-AiBA) is eluted.

Abstract: Both a column having high-pressure resistance and capable of performing high-speed separation and analysis even with a small flow rate and a liquid chromatograph apparatus using thereof are to be provided. A separation column according to the present invention has a monolithic rod being arranged in the center, being made of a porous material, and having a circular cross section, a filler layer arranged such that it encircles a circumferential surface of the monolithic rod, and a cylindrical support medium arranged outside the filler layer. The filler layer is formed by filling a tube-like gap between the monolithic rod and the support medium with particles or beads.

Abstract: This liquid feeding system has the first and second pumps each of which is provided with a plunger. Liquid sucked through each of the pump suction ports is discharged out of the discharging port through the first pump and/or second pump. Under the starting operation mode, the second pump is stopped and only the first pump is operated. When the discharging pressure at the discharging port reaches up to a predetermined value, the starting operation mode is changed over to the normal operation mode. Under the normal operation mode, the first pump is stopped and only the second pump is operated.

Abstract: Disclosed herein is a separation column comprising a porous block covered with a coating wherein space-free adhesion is attainable therebetween by low temperature process without releasing octadecylsilyl bases and others from the porous block. A monolith rod 1 is covered with a metal or resin coating 2. Then, the monolith rod 1 covered by the coating 2 is further covered with a rod retainer 3 made of a low melting point metal (melting point 100-200 degrees C.) to form a column body 19. Since the rod retainer 3 is formed by pressure molding at low temperature (100-200 degrees C.). It is therefore possible to absorb the surface irregularities of the monolith rod 1 by the coating 2 while octadecylsilyl bases and others from are released when the retainer 3 is formed. This allows the potential separation ability of the porous block to be brought out maximally.

Abstract: A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. The present invention is characterized by a chromatographic data processor for executing data processing of a chromatogram obtained by separating a sample to be measured using a column and detecting the separated sample, wherein fitting processing is executed to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front side of the time region or from the back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another. Thus, the plurality of overlapping peaks, particularly three or more overlapping peaks in the chromatogram can be easily separated from one another only by defining some setting conditions.

Abstract: A method for analyzing a plurality of amino acids in a fluid sample by a user is provided comprising the steps of introducing the sample into a buffer solution, passing the sample in the buffer solution through a separation column and setting a lithium ion concentration in the buffer to no more than 0.3 mols/L up to a time before ?-aminoisobutyric acid (?-AiBA) is eluted.

Abstract: The present invention provides a separation column and a liquid chromatograph using the separation column, which are capable of carrying out a high-speed analysis under high pressure and without a reduction in separation performance of the separation column. In the separation column, a sample and a mobile phase are introduced into a packing material. The chromatograph has a covering member for covering an outer surface of the packing material and a supporting member having the packing material covered with the covering member inserted therein, wherein the level of pressure generated within a gap located between the covering member and the supporting member on the outflow side of the gap, where the fluid is introduced, is lower than that of pressure generated within the gap on the inflow side of the gap, where the fluid flows out of the gap.

Abstract: A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. The present invention is characterized by a chromatographic data processor for executing data processing of a chromatogram obtained by separating a sample to be measured using a column and detecting the separated sample, wherein fitting processing is executed to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front side of the time region or from the back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another. Thus, the plurality of overlapping peaks, particularly three or more overlapping peaks in the chromatogram can be easily separated from one another only by defining some setting conditions.

Abstract: A method for analyzing a multi-channel chromatogram is realized for accurately resolving overlapping peaks on a multi-channel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.

Abstract: The flow rate of a solvent is reduced as a separation column and the separation performance is improved even under high-pressure conditions. There is provided a separation column including a monolith rod into which a sample and a mobile phase flow, the separation column comprising: a coating material coated on the outer circumference of a monolith rod; a support member into which the monolith rod coated with the coating material is inserted, and a rod fixing material fitted into or filled a gap between the coating material and the support member; wherein the upper end face of the rod fixing material is sealed, the upper end face being the inflow-side end when the separation column is assembled.

Abstract: A method for analyzing a multi-channel chromatogram is realized for accurately resolving overlapping peaks on a multi-channel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.

Abstract: This liquid feeding system has the first and second pumps each of which is provided with a plunger. Liquid sucked through each of the pump suction ports is discharged out of the discharging port through the first pump and/or second pump. Under the starting operation mode, the second pump is stopped and only the first pump is operated. When the discharging pressure at the discharging port reaches up to a predetermined value, the starting operation mode is changed over to the normal operation mode. Under the normal operation mode, the first pump is stopped and only the second pump is operated.

Abstract: There is provided a chromatograph analyzing device, in which results of a quantitative calculation process on an unseparated peak have less dispersion. The chromatograph analyzing device according to the present invention automatically executes a base line setting process on the unseparated peak using base line setting conditions set in advance.

Abstract: Compounds of the formula (I), wherein Z represents C—R7 or N, and R represents alkyl that may be optionally substituted, alkenyl, alkynyl, cycloalkyl or alkoxycarbonylamino, are useful as agricultural and horticultural fungicides.

Abstract: In a liquid chromatography apparatus, a separation column of intermediate stage is additionally connected between a separation column of first stage and a separation column of second stage. Preferably, a switching unit and a liquid feed unit for mixing and feeding a plurality of solutions are added to improve a separation capability. A three-dimensional liquid chromatography apparatus capable of avoiding the “solution interference” can be realized. Even a complex sample containing a hydrophilic component and a hydrophobic component in a mixed state can be separated and analyzed satisfactorily on-line.