Abstract: Both a column having high-pressure resistance and capable of performing high-speed separation and analysis even with a small flow rate and a liquid chromatograph apparatus using thereof are to be provided. A separation column according to the present invention has a monolithic rod being arranged in the center, being made of a porous material, and having a circular cross section, a filler layer arranged such that it encircles a circumferential surface of the monolithic rod, and a cylindrical support medium arranged outside the filler layer. The filler layer is formed by filling a tube-like gap between the monolithic rod and the support medium with particles or beads.

Abstract: This liquid feeding system has the first and second pumps each of which is provided with a plunger. Liquid sucked through each of the pump suction ports is discharged out of the discharging port through the first pump and/or second pump. Under the starting operation mode, the second pump is stopped and only the first pump is operated. When the discharging pressure at the discharging port reaches up to a predetermined value, the starting operation mode is changed over to the normal operation mode. Under the normal operation mode, the first pump is stopped and only the second pump is operated.

Abstract: Disclosed herein is a separation column comprising a porous block covered with a coating wherein space-free adhesion is attainable therebetween by low temperature process without releasing octadecylsilyl bases and others from the porous block. A monolith rod 1 is covered with a metal or resin coating 2. Then, the monolith rod 1 covered by the coating 2 is further covered with a rod retainer 3 made of a low melting point metal (melting point 100-200 degrees C.) to form a column body 19. Since the rod retainer 3 is formed by pressure molding at low temperature (100-200 degrees C.). It is therefore possible to absorb the surface irregularities of the monolith rod 1 by the coating 2 while octadecylsilyl bases and others from are released when the retainer 3 is formed. This allows the potential separation ability of the porous block to be brought out maximally.

Abstract: A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. The present invention is characterized by a chromatographic data processor for executing data processing of a chromatogram obtained by separating a sample to be measured using a column and detecting the separated sample, wherein fitting processing is executed to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front side of the time region or from the back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another. Thus, the plurality of overlapping peaks, particularly three or more overlapping peaks in the chromatogram can be easily separated from one another only by defining some setting conditions.

Abstract: A method for analyzing a plurality of amino acids in a fluid sample by a user is provided comprising the steps of introducing the sample into a buffer solution, passing the sample in the buffer solution through a separation column and setting a lithium ion concentration in the buffer to no more than 0.3 mols/L up to a time before ?-aminoisobutyric acid (?-AiBA) is eluted.

Abstract: The present invention provides a separation column and a liquid chromatograph using the separation column, which are capable of carrying out a high-speed analysis under high pressure and without a reduction in separation performance of the separation column. In the separation column, a sample and a mobile phase are introduced into a packing material. The chromatograph has a covering member for covering an outer surface of the packing material and a supporting member having the packing material covered with the covering member inserted therein, wherein the level of pressure generated within a gap located between the covering member and the supporting member on the outflow side of the gap, where the fluid is introduced, is lower than that of pressure generated within the gap on the inflow side of the gap, where the fluid flows out of the gap.

Abstract: A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. The present invention is characterized by a chromatographic data processor for executing data processing of a chromatogram obtained by separating a sample to be measured using a column and detecting the separated sample, wherein fitting processing is executed to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front side of the time region or from the back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another. Thus, the plurality of overlapping peaks, particularly three or more overlapping peaks in the chromatogram can be easily separated from one another only by defining some setting conditions.

Abstract: A method for analyzing a multi-channel chromatogram is realized for accurately resolving overlapping peaks on a multi-channel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.

Abstract: The flow rate of a solvent is reduced as a separation column and the separation performance is improved even under high-pressure conditions. There is provided a separation column including a monolith rod into which a sample and a mobile phase flow, the separation column comprising: a coating material coated on the outer circumference of a monolith rod; a support member into which the monolith rod coated with the coating material is inserted, and a rod fixing material fitted into or filled a gap between the coating material and the support member; wherein the upper end face of the rod fixing material is sealed, the upper end face being the inflow-side end when the separation column is assembled.

Abstract: A method for analyzing a multi-channel chromatogram is realized for accurately resolving overlapping peaks on a multi-channel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.

Abstract: This liquid feeding system has the first and second pumps each of which is provided with a plunger. Liquid sucked through each of the pump suction ports is discharged out of the discharging port through the first pump and/or second pump. Under the starting operation mode, the second pump is stopped and only the first pump is operated. When the discharging pressure at the discharging port reaches up to a predetermined value, the starting operation mode is changed over to the normal operation mode. Under the normal operation mode, the first pump is stopped and only the second pump is operated.

Abstract: There is provided a chromatograph analyzing device, in which results of a quantitative calculation process on an unseparated peak have less dispersion. The chromatograph analyzing device according to the present invention automatically executes a base line setting process on the unseparated peak using base line setting conditions set in advance.

Abstract: Compounds of the formula (I), wherein Z represents C—R7 or N, and R represents alkyl that may be optionally substituted, alkenyl, alkynyl, cycloalkyl or alkoxycarbonylamino, are useful as agricultural and horticultural fungicides.

Abstract: In a liquid chromatography apparatus, a separation column of intermediate stage is additionally connected between a separation column of first stage and a separation column of second stage. Preferably, a switching unit and a liquid feed unit for mixing and feeding a plurality of solutions are added to improve a separation capability. A three-dimensional liquid chromatography apparatus capable of avoiding the “solution interference” can be realized. Even a complex sample containing a hydrophilic component and a hydrophobic component in a mixed state can be separated and analyzed satisfactorily on-line.

Abstract: A chromatographic analyzer is provided for facilitating curve fitting by means of the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. The present invention is characterized by a chromatographic data processor for executing data processing of a chromatogram obtained by separating a sample to be measured using a column and detecting the separated sample, wherein fitting processing is executed to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front side of the time region or from the back side of the time region, and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another. Thus, the plurality of overlapping peaks, particularly three or more overlapping peaks in the chromatogram can be easily separated from one another only by defining some setting conditions.

Abstract: A pump device is realized, which accurately detects a flow rate of a pump, performs real-time control of the flow rate, and thus can suppress a flow rate error caused by occurrence of pulsation. Control of a pump rotation speed by a pressure sensor has low responsiveness in a low pressure region, and a correlation between a pressure and a flow rate is incomplete in a high flow rate region. With this taken into consideration, a flow rate sensor 10 is disposed at an outlet port 9 of a second pump 4. A position detection sensor 14 detects a point where a first pump 3 is shifted from a suction mode to a discharge mode. Moreover, the flow rate sensor 10 detects that there is no discharge from the pump 3, and that there is discharge from the pump 4 alone. Accordingly, a controller 15 sets a rotation speed of a motor 12 to a speed double the previous speed (double the normal rotation speed).

Abstract: A chromatographic analyzer is provided for facilitating curve filling by the linear least-square method for a chromatogram that contains a plurality of overlapping peaks. A chromatographic data processor executes fitting processing to each peak in an arbitrary time region having the plurality of peaks of the chromatogram starting from the front or back side of time region and the processed peaks are subtracted from the chromatogram in the time region so that the plurality of peaks in the chromatogram can be separated from one another.

Abstract: The invention provides a pump for liquid chromatography excellent in feeding liquid stably at an extremely low flow rate and in discharging bubbles at startup. In a pump for liquid chromatography including a cylinder and a plunger that reciprocates in the cylinder to suck and discharge fluid, the pump further includes a large-flow-rate pump that feeds liquid by the plunger, a small-flow-rate pump that feeds the liquid by the plunger, motion conversion means that converts the rotational motion of a motor to a reciprocating motion, an actuator that directly drives the plunger, and a drive part that drives the actuator, and selectively switches means for driving the plunger.

Abstract: There is provided a user interface which enables all users to input purpose of use of a chromatography data system, recognize a series of operation flow of the analysis of chromatograph data according to the input purpose, improve an operability and prevent an operation error regardless of the user's knowledge on analyzing the chromatograph data or the user's proficiency in the chromatography data system. In a chromatograph data processing, the present invention relates to recognizing chromatograph data analyzing means on a picture and displaying the chromatograph data analyzing means on the picture in accordance with purpose of use. According to the present invention, the chromatography data system can be easily operated regardless of the knowledge on the analysis of chromatograph data or proficiency in the chromatography data system.

Abstract: A separation analyzer for transferring solutions, while changing mixing ratios of the solutions, wherein a flow passage from a first pump, a flow passage from a second pump, and a passage for sample introduction section, the flow passages being connected, which further comprises a flow switching means for temporarily storing the solutions, whereby the flow switching means alternatively makes a first state for pushing out the solutions in a second sampling loop to the sample introduction section by the second pump, while transferring the solutions to the first sampling loop; and a second state for pushing out the solutions in the first sampling loop by the second pump, while transferring the solutions from the first pump to the second sampling loop.