Abstract: The present invention relates to a method for the analysis of methylated cytosines in DNA. In the first step of the invention unmethylated cytosines in the DNA to be analysed are chemically converted into uracil while 5-methylcytosines remain unchanged. In a second step a methylation specific oligonucleotide carrying a non-extendable 3? end is annealed to the converted DNA. Subsequently, the non-extendable 3? terminus of the oligonucleotide is removed in case the oligonucleotide is bound to the DNA with the methylation status to be detected. Finally the unblocked oligonucleotide is extended, and the methylation status is concluded from the absence or presence of an extended oligonucleotide product. The method is preferably used for diagnosis and/or prognosis of adverse events for individuals, for distinguishing cell types and tissues, or for investigating cell differentiation.

Abstract: The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among liver cell proliferative disorders or for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

Abstract: A multimodal biometric method is provided for authenticating at least one user, in which at least one user is assigned to one of at least two classes, in which similarity measures are used that are measures of the similarity of biometric data, determined for the user, to reference data for one of the classes, in which a overall statistical statement on the user's membership of the class is present for each of the at least two classes as a function of the similarity measure, in which the overall statistical statement for each of the at least two classes results from the combination of the statistical statements of at least two individual biometries for the respective class, the statistical statements of the individual biometries being statistical statements on the user's membership of the class as a function of the similarity measures, and in which the user is assigned to one of at least two classes when a specific assignment criterion is fulfilled.

Abstract: The present invention describes a method for the detection of nucleic acid sequences, which is characterized in that the following steps are conducted: a) at least one nucleic acid is bound to a solid phase; b) probe molecules are hybridized to the nucleic acids in a sequence-specific manner, whereby the probe molecules are provided with a cleavable bond and a mass label, which is specific for the probe molecule; c) removal of the unhybridized probe molecules; d) contacting of the hybridized probe molecules with a matrix, which cleaves said cleavable bonds and at the same time serves as the matrix in a MALDI mass spectrometer; e) detection of the mass label at those positions where the nucleic acid was bound.

Abstract: In a method for producing an air restrictor, use is made of at least one liquid or tacky substance to join perforated plates to one another and to seal the air restrictor. The substance can be, for example, an adhesive contained in capsules. The adhesive is liberated by bursting the capsules inserted between the perforated plates.

Abstract: Particular aspects of the present invention provide a method for quantification of two different variations of a DNA sequence. Particularly, the invention relates to a quantification of methylated DNA, and for this purpose, the test DNA is converted so that cytosine is converted to uracil, while 5-methylcytosine remains unchanged. The converted DNA is amplified by means of a real-time PCR, wherein two labeled real-time probe types are utilized: one specific for methylated DNA; and one for unmethylated DNA. Preferably, the degree of methylation of the test DNA is calculated from the ratio of the signal intensities of the probes or from the Ct values. The inventive methods have substantial utility for diagnosis and prognosis of cancer and other disorders associated with altered or characteristic DNA methylation status, as well as having substantial utility for analysis of SNPs, allelic expression, and prediction of drug response, drug interactions, among other uses.

Abstract: A device for picking up a sheet trailing edge from an upstream cylinder and transferring the sheet trailing edge to a gripper system of a downstream drum, as viewed in sheet travel direction, includes a sucker guide mechanism having a movably mounted sucker carrier whereon sucker heads subjectible to an application of vacuum or suction air are disposed. The sucker carrier is constructed as a tubular part having a longitudinal axis and being movable perpendicularly to the longitudinal axis thereof. The tubular part is formed with a vacuum or suction air duct extending in the interior thereof and is formed of carbon fiber-reinforced plastic-material laminate.

Abstract: The invention relates to a composite material, made from a microfilament nonwoven fabric having a mass per unit area of 40 to 200 g/m2, in which a nonwoven fabric, which is made from melt-spun, drawn, multi-component endless filaments having a titer of 1.5 to 5 dtex and directly laid up to form a fibrous web, and in which the multi-component endless filaments, optionally after prebonding, are split at least to 80% to form micro-endless filaments having a titer of 0.1 to 1.2 dtex and bonded, is bonded to a thermoplastic synthetic material using an injection-molding process.

Abstract: The invention relates to a sound absorbing material, made from a microfilament nonwoven fabric having a mass per unit area of 40 to 300 g/m2, in which a nonwoven fabric is made from melt-spun, drawn, multi-component endless filament having a titer of 1.5 to 5 dtex and directly laid down to form a fibrous web; and the multi-component endless filaments, optionally after prebonding, are split at least to 80% to form micro-endless filaments having a titer of 0.1 to 1.2 dtex and bonded.

Abstract: A device for picking up a sheet trailing edge from an upstream cylinder and transferring the sheet trailing edge to a gripper system of a downstream drum, as viewed in sheet travel direction, includes a sucker guide mechanism having a movably mounted sucker carrier whereon sucker heads subjectible to an application of vacuum or suction air are disposed. The sucker carrier is constructed as a tubular part having a longitudinal axis and being movable perpendicularly to the longitudinal axis thereof. The tubular part-is formed with a vacuum or suction air duct extending in the interior thereof and is formed of carbon fiber-reinforced plastic-material laminate.

Abstract: A device for hanging a load on a helicopter has at least one load-bearing cable which has a first cable section, which is off-center in relation to a longitudinal axis of the helicopter, and a second off-center cable section located opposite. The load-bearing cable is connected to the helicopter, on the one hand, and to the load, on the other hand. Ends and of the load-bearing cable are fixedly attached to the helicopter, and the load-bearing cable is connected to the load in a manner in which it can be moved relative to the latter, such that the load can be displaced along the load-bearing cable.

Abstract: The invention relates to a method for scaling similarity measures for a biometric method, the similarity measures being measures of the similarity of biometric data, to be compared in the biometric method, of a user with reference data of users. The similarity measures are scaled with the aid of mean value scaling.

Abstract: A multimodal biometric method is provided for authenticating at least one user, in which at least one user is assigned to one of at least two classes, in which similarity measures are used that are measures of the similarity of biometric data, determined for the user, to reference data for one of the classes, in which a overall statistical statement on the user's membership of the class is present for each of the at least two classes as a function of the similarity measure, in which the overall statistical statement for each of the at least two classes results from the combination of the statistical statements of at least two individual biometries for the respective class, the statistical statements of the individual biometries being statistical statements on the user's membership of the class as a function of the similarity measures, and in which the user is assigned to one of at least two classes when a specific assignment criterion is fulfilled.

Abstract: A device for hanging a load on a helicopter has at least one load-bearing cable which has a first cable section, which is off-center in relation to a longitudinal axis of the helicopter, and a second off-center cable section located opposite. The load-bearing cable is connected to the helicopter, on the one hand, and to the load, on the other hand. Ends and of the load-bearing cable are fixedly attached to the helicopter, and the load-bearing cable is connected to the load in a manner in which it can be moved relative to the latter, such that the load can be displaced along the load-bearing cable.

Abstract: A cartridge filter has a filter element and a frame formed of plates. The plates are installable around the filter element and are interconnectable in the area of the sides of the plates. The plates have plug-in connection members which interact with corresponding plug-in connection members of adjacent plates to thereby affix the plates to one another.

Abstract: A cartridge filter has a filter element and a frame formed of plates. The plates are installable around the filter element and are interconnectable in the area of the sides of the plates. The plates have plug-in connection members which interact with corresponding plug-in connection members of adjacent plates to thereby affix the plates to one another.

Abstract: A sheet-guiding device with a cooled sheet-guiding plate formed with air outlet openings, the sheet-guiding plate having a sheet-guiding surface on one side thereof and being connected, at the other side thereof opposite to the one side, to coolant ducts permitting the air outlet openings to remain freely open, and a cooling-duct plate having deformations forming the coolant ducts, the cooling-duct plate being connected by welding to the sheet-guiding plate and being sealed with respect thereto, includes a sealing element disposed between the other side of the sheet-guiding plate and the cooling-duct plate, the sealing element being in a groove-shaped indentation formed in the cooling-duct plate and surrounding a respective air outlet opening, and welding zones located in the vicinity of the sealing element for forming a connection between the sheet-guiding plate and the cooling-duct plate by weldments.

Abstract: A process for the synthesis of a glycopeptide using a solid phase matrix is disclosed. The matrix is compatible with aqueous and organic solvents and is comprised of a silica-based solid support to which is linked a two-part spacer group having a chain length of about 12 to about 40 methylene groups. The first part of the spacer is covalently bonded to the silica-based support and has a length of about 3 to about 10 methylene groups. The second spacer part is covalently bonded to the first part of the spacer and comprises the distal end of the two part spacer. The second part is soluble as a free molecule in each of water, dimethylformamide and dichloromethane and has a terminal amine or hydroxyl group to which the C-terminal residue of the peptide portion of the glycopeptide chain is bonded.

Abstract: The invention relates to a process for preparing chirally uniform peptides. The aim of the invention is the preparation of chirally uniform peptides with the aid of proteolytic enzymes. The object comprises reacting alkyl esters of N-acylamino acids with proteases with amino acids, amino acid derivatives or peptides. The object is achieved by reacting an ester of an N-acylamino acid with a suitable amino acid derivative or peptide derivative with an unprotected N-terminal alphaamino group in frozen aqueous solution during catalysis with a serine protease or cysteine protease.