Patents by Inventor Mehdi Azimi

Mehdi Azimi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20120028842
    Abstract: A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequence, and, an array of probes each having a nucleic acid sequence for hybridization with the target nucleic acid sequence to form a probe-target hybrid, wherein, the array of probes includes a control probe for hybridization with a target sequence known to be always present in the biological sample.
    Type: Application
    Filed: June 1, 2011
    Publication date: February 2, 2012
    Inventors: KIA SILVERBROOK, MEHDI AZIMI
  • Publication number: 20120004145
    Abstract: An oligonucleotide spotting robot for spotting oligonucleotide probes into a silicon wafer on which an array of lab-on-a-chip (LOC) devices are fabricated, each LOC device having a digital memory for data related to the reagents loaded into the LOC device, the oligonucleotide dispensing robot having an array of reservoirs for containing the oligonucleotide probes suspended in a liquid, an array of ejectors, each of the ejectors being configured for fluid communication with a corresponding one of the reservoirs respectively, a mounting surface for detachably mounting the array of LOC devices for movement relative to the ejectors, and, a control processor for operative control of the ejectors and the mounting surface, wherein, the control processor is configured to activate the ejectors, move the ejectors selected for activation into registration with one or more of the LOC devices and download the data specifically relevant to each of the LOC devices into the digital memory of that LOC device.
    Type: Application
    Filed: June 1, 2011
    Publication date: January 5, 2012
    Inventors: Mehdi Azimi, Kia Silverbrook
  • Publication number: 20110312705
    Abstract: A test module for amplifying nucleic acid sequences in a sample fluid, the test module having an outer casing with receptacle for receiving the sample fluid, a polymerase chain reaction (PCR) section with a PCR microchannel for thermally cycling a PCR mixture of the sample fluid, polymerase, dNTP's, primers, and buffer solution to amplify the nucleic acid sequences, wherein, the PCR mixture has a volume less than 400 nanoliters.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Mehdi Azimi, Geoffrey Richard Facer, Kia Silverbrook
  • Publication number: 20110312626
    Abstract: A test module having an outer casing having a receptacle for receiving a biological sample, an excitation source mounted in the outer casing for sequentially illuminating the biological sample with different wavelengths of light, and, a photosensor positioned to detect the different wavelengths of light transmitted through the biological sample, wherein during use, the photosensor output signal is used to generate a spectrogram for analysis of a characteristic of the biological sample.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Mehdi Azimi, Kia Silverbrook, Alireza Moini
  • Publication number: 20110312556
    Abstract: A microfluidic device having a supporting substrate, a hybridization chamber containing a probe having a nucleic acid sequence for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid being configured to generate a fluorescence signal in response to an excitation light, and, CMOS circuitry between the supporting substrate and the hybridization chamber, the CMOS circuitry having a photodiode for generating an output signal in response to the fluorescence signal, and a trigger photodiode for generating an output in response to the excitation light, wherein, the CMOS circuitry is configured to activate the photodiode when the trigger photodiode indicates the excitation light has deactivated.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Alireza Moini, Mehdi Azimi
  • Publication number: 20110312567
    Abstract: A lab-on-a-chip (LOC) device for detecting target nucleic acid sequences in a fluid, the LOC device having electrochemiluminescent (ECL) probes for detecting the target nucleic acid sequences, each of the probes having an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, electrodes for receiving an electrical pulse to excite the ECL luminophores, hybridization chambers containing the probes for detection of the targets, and a pair of the electrodes, and, at least one negative control chamber without the ECL probes.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Alireza Moini, Mehdi Azimi, Kia Silverbrook
  • Publication number: 20110312784
    Abstract: A microfluidic device for detecting target molecules in a sample, the microfluidic device having probes for reaction with the target molecules to form probe-target complexes, the probe-target complexes being configured to emit photons when excited, photodiodes for detecting photons emitted by the probe-target complexes and a calibration photodiode for exposure to a calibration source to generate a calibration signal, wherein during use, output from the photodiodes for detecting photons emitted by the probe-target complexes is calibrated by the calibration signal.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Alireza Moini, Mehdi Azimi, Kia Silverbrook
  • Publication number: 20110312585
    Abstract: A microfluidic device for amplifying DNA and RNA, the microfluidic device having an inlet for receiving a sample containing genetic material including DNA and RNA, a plurality of reagent reservoirs containing reagents for addition to the sample, a first nucleic acid amplification section for amplifying at least some of the genetic material, and, a second nucleic acid amplification section for amplifying at least some of the genetic material in parallel with the first nucleic acid amplification section.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi, Geoffrey Richard Facer
  • Publication number: 20110312790
    Abstract: A microfluidic test module having an outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, a hybridization chamber mounted in the casing, the hybridization chamber containing probes having a nucleic acid sequence for hybridization with the target nucleic acid sequence to form probe-target hybrids, wherein, the hybridization chamber has a volume less than 900,000 cubic microns.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312637
    Abstract: A lab-on-a-chip (LOC) device to separate pathogens from a biological sample for analysis, the LOC device having a supporting substrate, an inlet for receiving the sample including pathogens and other constituents larger than the pathogens, a dialysis section for separating the pathogens into a portion of the sample, and, a microsystems technology (MST) layer for separately analyzing the pathogens, wherein, the inlet, the dialysis section and the MST layer are all supported on the supporting substrate.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312079
    Abstract: A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry with digital memory for storing epidemiological data, and configured to download epidemiological data updates from an external source.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi, Matthew Taylor Worsman
  • Publication number: 20110312734
    Abstract: A test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing having an inlet for receiving the fluid containing the target nucleic acid sequences, electrode pairs for receiving an electrical pulse, electrochemiluminescent (ECL) probes adjacent each of the electrode pairs respectively, each of the ECL probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, such that the electrical pulse to the electrode pair excites the ECL luminophores, wherein, the ECL probes are not anchored to a surface and form a suspension in the fluid containing the target nucleic acid sequences during detection.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312742
    Abstract: A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing a target nucleic acid sequence, a probe with a nucleic acid sequence for hybridization with the target nucleic acid sequence to form a probe-target hybrid and generate a fluorescence signal in response to an excitation light, and, a photosensor for sensing the fluorescence signal, wherein during use, addition of the biological sample to the inlet prevents fluid communication between a fluid subsequently added to the inlet and the probe.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Alireza Moini, Mehdi Azimi
  • Publication number: 20110312715
    Abstract: A microfluidic test module for detecting target nucleic acid sequences in a fluid, the test module having an outer casing having an inlet for receiving the fluid containing the target nucleic acid sequences, electrode pairs for receiving an electrical pulse, electrochemiluminescent (ECL) probe spots in contact with each of the electrode pairs respectively, the ECL probe spots containing ECL probes having an ECL luminophore for emitting photons when in an excited state and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, such that the electrical pulse to the electrode pair excites the ECL luminophores, wherein, the mass of the ECL probes in each of the probe spots is less than 270 picograms.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312558
    Abstract: A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, a first nucleic acid amplification section downstream of the lysis section for amplifying first nucleic acid sequences in the genetic material, and, a second nucleic acid amplification section downstream of the first nucleic acid amplification section for amplifying second nucleic acid sequences in the amplicon from the first nucleic acid amplification section, wherein, the dialysis section, the lysis section, the first nucleic acid amplification section and the second nucleic acid amplification section are all supported on the supporting substrate.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: MEHDI AZIMI, GEOFFREY RICHARD FACER, ALIREZA MOINI, KIA SILVERBROOK
  • Publication number: 20110312564
    Abstract: A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, and, a nucleic acid amplification section downstream of the incubation section for amplifying nucleic acid
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Mehdi Azimi, Geoffrey Richard Facer, Alireza Moini, Kia Silverbrook
  • Publication number: 20110312068
    Abstract: A dialysis device to separate nucleated cells in a biological sample from smaller constituents, the dialysis device having a first channel for receiving the biological sample, a second channel, and, a plurality of apertures, wherein, the second channel is fluidically connected to the first channel via the apertures, the apertures being smaller than the nucleated cells such that the smaller constituents flow from the first channel to the second channel and the nucleated cells in the biological sample remain in the first channel
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Mehdi Azimi, Kia Silverbrook
  • Publication number: 20110312716
    Abstract: A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing buffer solution, dNTPs, primers, and polymerase for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for maintaining the amplification mixture at a predetermined temperature during isothermal amplification of the target nucleic acid sequences.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi, Geoffrey Richard Facer
  • Publication number: 20110312552
    Abstract: A microfluidic device for processing a fluid, the microfluidic device having an inlet for receiving the fluid, functional sections for processing the fluid, a flow-path extending from the inlet into at least some of the functional sections, CMOS circuitry for operative control of the functional sections, and, a conductivity sensor with a first terminal and a second terminal spaced apart along the flow-path, and a first electrode and a second electrode positioned between the first terminal and the second terminal and spaced apart along the flow-path, wherein, the CMOS circuitry is configured to generate a current between the first terminal and the second terminal, and measure a voltage across the first electrode and the second electrode such that conductivity of the fluid in the flow-path is derived from the current and the measured voltage.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Mehdi Azimi, Kia Silverbrook
  • Publication number: 20110312791
    Abstract: A test module for genetic analysis having a casing for hand held portability, an inlet for receiving a liquid containing nucleic acid sequences, an outlet downstream of the inlet, and, a fault-tolerant multiple valve assembly having a plurality of flow-paths extending from the inlet to the outlet and a plurality of valves positioned along each of the flow-paths respectively.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi, Matthew Taylor Worsman