Patents by Inventor Mehdi Azimi

Mehdi Azimi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20110312568
    Abstract: A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating cells larger than a predetermined threshold in the sample from smaller constituents, whereby the cells larger than a predetermined threshold include target cells containing genetic material for analysis, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the target cells to release the genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the target cells in the lysis section, an incubation section downstream of the lysis section, the incubation section being in fluid communication with one of the reagent reservoirs containing enzymes for enzymatic reaction with the genetic material, a first nucleic acid amplification section downstream of the incubation section for amplifying first nucle
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Alireza Moini, Kia Silverbrook, Mehdi Azimi, Geoffrey Richard Facer
  • Publication number: 20110312703
    Abstract: A microfluidic device having a supporting substrate, a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having a PCR chamber, and, a heater element for heating the nucleic acid sequences within the PCR chamber, wherein during use, the PCR section generates sufficient amplicon for hybridization with an array of probes in less than 10 minutes.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Geoffrey Richard Facer, Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312773
    Abstract: A microfluidic device having an inlet for receiving a liquid flowing through the microfluidic device, an outlet downstream of the inlet, and, a fault-tolerant multiple valve assembly having a plurality of flow-paths extending from the inlet to the outlet and a plurality of valves positioned along each of the flow-paths respectively.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi, Matthew Taylor Worsman
  • Publication number: 20110312748
    Abstract: A microfluidic device having a supporting substrate, an array of probes for hybridization with a target nucleic acid sequence to form a probe-target hybrid and generate a fluorescence signal in response to an excitation light, and, a photosensor positioned adjacent the array of probes, wherein, the photosensor is configured for sensing which probes within the array of probes generated the fluorescence signal.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: ALIREZA MOINI, MEHDI AZIMI, KIA SILVERBROOK
  • Publication number: 20110312677
    Abstract: A lab-on-a-chip (LOC) device for detecting target molecules in a fluid, the LOC device having an array of electrochemiluminescent (ECL) probes for reaction with the target molecules to form probe-target complexes, and, electrodes for receiving an electrical pulse, wherein, the probes each have an ECL luminophore that emits a photon when excited by current between the electrodes, and a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Alireza Moini, Mehdi Azimi, Kia Silverbrook
  • Publication number: 20110312834
    Abstract: A lab-on-a-chip (LOC) device for detecting a target nucleic acid sequence in a sample, the LOC device having a supporting substrate, probes with a nucleic acid sequence complementary to the target nucleic acid sequence for forming probe-target hybrids, and a ruthenium organic complex, electrodes for generating an excited state in the ruthenium organic complex in which the ruthenium organic complex emits photons of light, and, CMOS circuitry for applying a voltage across the electrodes.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi, Geoffrey Richard Facer, Alireza Moini
  • Publication number: 20110312679
    Abstract: A microfluidic device for removing cell debris from a biological sample, the microfluidic device having a dialysis section with a large constituent channel, a small constituent channel and a plurality of apertures for fluid communication between the large constituent channel and the small constituent channel, the large constituent channel having an upstream end for receiving the biological sample, the biological sample being a liquid carrying a mixture of cell debris and target molecules, the small constituent channel having a downstream end for connection to a hybridization section with an array of probes for reaction with the target molecules to form probe-target complexes, wherein, the apertures are spaced along the large constituent channel at a spacing between 1 micron and 10 microns and each of the apertures is sized to allow the target molecules to flow into the small constituent channel but retain the cell debris larger than a threshold size in the large constituent channel.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Mehdi Azimi, Kia Silverbrook
  • Publication number: 20110312633
    Abstract: A microfluidic device for dialysis of biological material, the microfluidic device having a sample inlet for receiving a sample of biological material that has constituents of different sizes, a dialysis section for separating smaller constituents from larger constituents, the smaller constituents being smaller than a predetermined size threshold, and the larger constituents being larger than the predetermined size threshold, and, a microsystems technology (MST) layer for separately processing the smaller constituents and/or the larger constituents.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312800
    Abstract: A test module for analyzing genetic material in a biological sample, the test module having an outer casing with a receptacle for receiving the biological sample, functional sections for processing and analyzing the biological sample, a controller for operating the functional sections, and, a flow-path from the receptacle to the functional sections, wherein, the flow-path is configured to draw the sample to be analyzed from the receptacle to the functional sections by capillary action regardless of module orientation relative to gravity.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Alireza Moini, Mehdi Azimi
  • Publication number: 20110312652
    Abstract: A microfluidic device for detecting target nucleic acid sequences in a sample, the microfluidic device having an array of hybridization chambers, each of the hybridization chambers containing electrode pairs for receiving an electrical pulse and electrochemiluminescent (ECL) probes for hybridization with the target nucleic acid sequences, the ECL probes being configured to emit a photon of light when hybridized with one of the nucleic acid targets and excited by current between the electrodes, wherein, the mass of the probes in each of the hybridization chambers is less than 270 picograms.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312635
    Abstract: A microfluidic device for processing a fluid sample, the microfluidic device having a first channel configured to fill with the sample by capillary action, a second channel configured to fill with the sample by capillary action, a plurality of fluid connections between the first channel and the second channel, each of the fluid connections being configured to pin a meniscus of the sample that arrests capillary flow between the first channel and the second channel, a bypass channel between the first channel and the second channel, the bypass channel joining the second channel upstream of the plurality of fluid connections and is configured for uninterrupted capillary driven flow from the first channel to the second channel, wherein during use, flow from the bypass channel reaches the meniscus pinned at each of the fluid connections after the meniscus has formed such that the flow sequentially removes each of the menisci and sample flow from the first channel to the second channel is via the plurality of fluid
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312544
    Abstract: A microfluidic device having a supporting substrate, an inlet for receiving a biological sample containing target nucleic acid sequences, hybridization chambers containing probes that each have a nucleic acid sequence for hybridization with the target nucleic acid sequences to form probe-target hybrids, a fluorophore and a quencher configured such that the fluorophore emits a fluorescence signal in response to an excitation light and the quencher quenches the fluorescence signal when the probe is not hybridized, but fails to quench the fluorescence signal from the probe-target hybrid, and, a calibration chamber with no fluorophore.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312682
    Abstract: A lab-on-a-chip (LOC) device for amplifying and detecting target nucleic acid sequences, the LOC device having electrochemiluminescent (ECL), resonant energy transfer, stem-and-loop probes for hybridization with the target nucleic acid sequences, each of the probes having a loop portion containing a sequence complementary to the target nucleic acid sequence, an ECL luminophore for emitting photons when in an excited state, a functional moiety for quenching photon emission from the ECL luminophore by resonant energy transfer, and a covalently attached primer for extension along a complementary sequence denatured from the target nucleic acid sequence to replicate the target nucleic acid sequence, heaters for thermally cycling the target nucleic acid sequences through a polymerase chain reaction (PCR), in which the covalently attached primers anneal to oligonucleotides containing the target nucleic acid sequences, and, electrodes for receiving an electrical pulse to excite the ECL luminophores, wherein during us
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Alireza Moini, Mehdi Azimi, Kia Silverbrook
  • Publication number: 20110312690
    Abstract: A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having a microchannel configured to have a plurality of mutually parallel, adjacent channel sections, and a plurality of elongate heaters positioned end to end along each of the channel sections, wherein, each of the heaters are independently operable for two-dimensional control of heat flux density to the PCR section.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi, Geoffrey Richard Facer
  • Publication number: 20110312826
    Abstract: A test module having an outer casing dimensioned for hand-held portability, the outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, probes in the outer casing for hybridization with a target nucleic acid sequence to form probe-target hybrids, the probe-target hybrids being configured to generate a fluorescence signal in response to an excitation light, a laser positioned in the outer casing for generating the excitation light.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312601
    Abstract: A lab-on-a-chip (LOC) device for analyzing a sample fluid, the LOC device having a sample inlet for receiving the sample, a microsystems technology (MST) layer with functional sections for processing and analyzing the sample, and, CMOS circuitry with digital memory for storing data and operational information to operatively control the functional sections during processing and analysis of the sample, wherein, the data is encrypted for secure communication with an external device.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Mehdi Azimi, Kia Silverbrook, Matthew Taylor Worsman
  • Publication number: 20110312602
    Abstract: A lab-on-a-chip (LOC) device for genetic analysis having a supporting substrate, an array of probes for hybridization with target nucleic acid sequences to form probe-target hybrids, a channel for directing a flow of liquid containing the target nucleic acid sequences, the channel having an inlet, an outlet and a meniscus anchor between the inlet and the outlet such that the flow from the inlet towards the outlet stops at the meniscus anchor where the liquid forms a meniscus, and, an actuator valve with a movable member for contacting the liquid, and an actuator for displacing the movable member from a quiescent position to an actuated position where the meniscus is extended into contact with a surface downstream of the meniscus anchor such that the liquid flow towards the outlet resumes.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Mehdi Azimi, Matthew Taylor Worsman, Kia Silverbrook
  • Publication number: 20110312856
    Abstract: An apparatus for dispensing reagents, loading oligonucleotide spotting devices and spotting oligonucleotide probes, the apparatus having a plurality of reagent vials, each with a droplet dispenser, a plurality of oligonucleotide vials, each with a droplet dispenser, a mounting surface for detachably mounting a microfluidic device for movement relative to the reagent vials, and detachably mounting an oligonucleotide spotting device, a chuck for detachably mounting the oligonucleotide spotting device adjacent the mounting surface, and, a control processor for operative control of the reagent and oligonucleotide vials, the oligonucleotide spotting device when mounted in the chuck and movement of the mounting surface relative to the reagent and oligonucleotide vials, and the oligonucleotide spotting device, wherein, the control processor is configured to activate any of the droplet dispensers, move the microfluidic device into registration with the reagent vials and move the oligonucleotide spotting device into r
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Mehdi Azimi
  • Publication number: 20110312762
    Abstract: A lab-on-a-chip (LOC) device having a supporting substrate, a microsystems technologies (MST) layer with a hybridization section that has an array of fluorescence resonance energy transfer (FRET) probes for hybridization with target nucleic acid sequences in a fluid, and, a photosensor for detecting hybridization of probes within the array of probes.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Mehdi Azimi, Kia Silverbrook, Alireza Moini
  • Publication number: 20110312795
    Abstract: A test module for detecting hybridization of probes with target nucleic acid sequences, the test module having a receptacle for receiving a biological sample with target nucleic acid sequences, an array of probes for hybridization with the target nucleic acid sequences, an excitation light emitting diode (LED) for illuminating the array of probes, CMOS circuitry having a photosensor for sensing a fluorescence emission from any of the probes in the array that have hybridized with the target nucleic acid sequences such that the CMOS circuitry is configured to use outputs from the photosensor to generate a signal indicative of the probes that have hybridized, wherein, the array of probes and the CMOS circuitry are integrated on a lab-on-a-chip (LOC) device contained within the test module.
    Type: Application
    Filed: June 1, 2011
    Publication date: December 22, 2011
    Inventors: Kia Silverbrook, Alireza Moini, Mehdi Azimi