Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: A pressure relief valve is provided that includes a housing and a flap. The housing includes a plurality of receptacle members with openings therebetween, and the flap is configured to be accepted by the housing and to contact the receptacle members in a closed position. Further, the flap covers the openings and inhibits airflow through the openings in a closed position, and the flap is biasable to allow airflow through the openings in an open position.

Abstract: A pressure relief valve flap includes an attachment portion, a main portion, and a hinge portion. The attachment portion is configured to be attached to the housing. The main portion has a lip and at least one damping feature. The lip extends around at least a portion of a periphery of the main portion. The hinge portion is disposed between and connects the main portion and the attachment portion. When the attachment portion is attached to the housing, the main portion is configured to move between a closed position and an opened position. When the main portion moves from the opened position to the closed position, the at least one damping feature is configured to engage the housing prior to the lip of the main portion and thereby dampen noise created when the lip of the main portion engages the housing.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: In an example, a circuit for controlling at least two electronic switches in a parallel configuration between a power supply and a load. The circuit includes a control circuit to generate first and second control signals to control first and second electronic switches of the at least two electronic switches, and establish a conduction sequence of the first and second electronic switches using the first and second control signals. The circuit includes a detection circuit configured to detect a current flowing through a control terminal of the first electronic switch during a transition portion, wherein the circuit is configured to adjust the first control signal and establish the second portion of the conduction sequence in response to the detected current.

Abstract: Systems, and methods are provided for the electronic purchase of gaming or amusement credits using a transaction device at a patron's location to apply funds from a financial account to the purchase of credits for use with an amusement or gaming device, system, or point of other wagering activity. Such credits are provided at the patron's location in the form of a printed ticket or other physical indicia of equivalent value, credits appearing in the credit meter and on the display of a gaming device, credit issued to a player card confirmed at the patron's location, credit issued to a debit card confirmed at the patron's location, and/or cash or other monetary-equivalents. In addition, physical confirmation of the completed electronic transaction from the patron's financial account may be concurrently provided to the patron in the form of a receipt.

Abstract: The present invention is directed to virus-like particles (VLPs) which are engineered to present epitopes from Staphylococcus aureus (SA), preferably autoinducing peptides (AIPs) which regulate quorum-sensing dependent virulence in this pathogen, or epitopes from SA toxins and leukocidins. These VLPs may be used to provide immunogenic compositions and efficacious vaccines. In a mouse model of SA dermonecrosis, vaccination with AIP-containing VLPs or SA toxin-containing VLPs induces protective immunity to limit the pathogenesis of SA infection and promote bacterial clearance.

Abstract: A delta-sigma modulator circuit comprising: an integrator circuit configured to produce an integrator output signal that represents an integration of an analog input signal, a comparator output signal and a periodic signal; a comparator circuit configured to produce the comparator output signal in response to a comparison of the integrator output signal with a first reference signal; and a periodic signal generation circuit configured to produce the periodic signal.

Abstract: The present invention relates generally to constructs and their use in gene expression or gene regulation assays. More particularly, the present invention provides expression vectors and/or reporter vectors providing kinetics of protein expression with improved temporal correlation to promoter activity. Even more particularly, the invention provides expression vectors comprising a transcribable polynucleotide which comprises a sequence of nucleotides encoding a RNA element that modulates the stability of a transcript corresponding to the transcribable polynucleotide. The present invention provides, inter alia, novel vectors, useful for identifying and analysing cis- and trans-acting regulatory sequences/factors as well as vectors and genetically modified cell lines or organisms that are particularly useful for drug screening and drug discovery.

Abstract: A power converter circuit can be operated in a constant-current mode when a monitored output current exceeds a specified over-current threshold. A value representing an output voltage of the power converter circuit can be compared to a soft-start voltage reference value. The soft-start voltage reference value can be set (or reset) to a value representing the output voltage of the power converter when the difference between the soft-start voltage reference value and the value representing the output voltage of the power converter is greater than a specified threshold. The output voltage of the power converter can be regulated using the soft-start voltage reference value as a target voltage including ramping the soft-start voltage reference value at specified rate to maintain the soft-start voltage reference value within a specified range of a specified regulated output voltage value, such as to constrain a slew rate of the regulated output voltage.

Abstract: A control circuit eliminates the Zeno phenomenon in a power supply controller while transferring control from a primary side controller to a secondary side controller. The primary side controller generates a feedback voltage (e.g., threshold voltage) that is input to a comparator. An output node voltage of the control circuit is fed back to the linear amplifier to be compared with the threshold voltage. While the output node voltage is less than the threshold voltage, a charge pump is coupled to the output node of the control circuit. After the output node voltage has toggled around the threshold voltage a number of times, the comparator output node is coupled to the output node of the control circuit and the charge pump is decoupled from the output node. When the output node voltage has discharged to be equal to the threshold voltage, control is handed off from the primary side controller to the secondary side controller.

Abstract: A client device, a system and a method for enabling financial transactions for an electronic gaming machine is described. The client device includes an electronic gaming machine processor, a printer, a printer sharing module and a controller. The printer sharing module is electrically coupled to the printer and the electronic gaming machine processor. The controller is electrically coupled to the printer sharing module. The controller reroutes the communications between the electronic gaming machine processor and the printer and allows the controller to communicate with the printer so that the controller instructs the printer to print a voucher. The system also includes a wireless communications module that enables communications with at least one other wireless communication module over short distances using point to point or broadcast packets that allow for bi-directional data transmission between each client device located on a casino gaming floor.

Abstract: A soft start-up method is provided comprising: producing an initialization signal on a primary winding side of a transformer; using a comparison of the initialization signal with a signal having a value indicative of a current in the primary winding to control a switch operatively disposed between a voltage source and the primary side; transmitting the initialization signal over a galvanically isolating communication medium from the primary side to a secondary winding side of a transformer; at the secondary winding side, comparing a reference voltage signal with a secondary winding output voltage signal; in response to a match between the reference signal and the secondary winding output voltage signal, transmitting a comparison voltage signal over the galvanically isolating communication medium from the secondary side to the primary side; at the primary side, comparing the initialization signal with the transmitted comparison voltage signal; and in response to a match between the initialization signal the an

Abstract: In an example, a circuit for controlling at least two electronic switches in a parallel configuration between a power supply and a load. The circuit includes a control circuit to generate first and second control signals to control first and second electronic switches of the at least two electronic switches, and establish a conduction sequence of the first and second electronic switches using the first and second control signals. The circuit includes a detection circuit configured to detect a current flowing through a control terminal of the first electronic switch during a transition portion, wherein the circuit is configured to adjust the first control signal and establish the second portion of the conduction sequence in response to the detected current.

Abstract: A client device, a system and a method for enabling financial transactions for an electronic gaming machine is described. The client device includes an electronic gaming machine processor, a printer, a printer sharing module and a controller. The printer sharing module is electrically coupled to the printer and the electronic gaming machine processor. The controller is electrically coupled to the printer sharing module. The controller reroutes the communications between the electronic gaming machine processor and the printer and allows the controller to communicate with the printer so that the controller instructs the printer to print a voucher. The system also includes a wireless communications module that enables communications with at least one other wireless communication module over short distances using point to point or broadcast packets that allow for bi-directional data transmission between each client device located on a casino gaming floor.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: A method and program product comprise communicating with a computing system. The computing system being configured to present a sports game and accept wagers on events. A selection of the sports game is communicated to the computing system. A play of the sports game is received. An overlay is received. The overlay comprises a grid structure. The grid structure comprises a plurality of locations arranged in at least a two-dimensional space. The space at least overlaying a playing area of the sports game, wherein a single position on the overlaid playing area is identified by a one of the plurality of locations. A user's wager is communicated to the computing system. The wager at least comprises an event and a one of the plurality of locations for occurrence of the event. An outcome of the wager is received from the computing system.

Abstract: The present invention is directed to the production, breeding and use of transgenic non-human animals such as mice in which specific genes, such as serum albumin, or portions of genes have been replaced by homologs from another animal, such as a human, to make the physiology of the animals so modified more like that of the other animal with respect to drug pharmacokinetics and metabolism. The invention also extends to the use of the genetically modified non-human animals of the invention for pharmacological and/or toxicological studies.

Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.