Patents by Inventor Michael Previte

Michael Previte has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230065693
    Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting nucleic acid sequencing workflows, where the workflows include library preparation, immobilization and amplification of the library molecules to form immobilized template molecules, and sequencing the template molecules. In some embodiments, the compositions comprise reagents used to conduct nucleic acid sequencing workflows.
    Type: Application
    Filed: September 19, 2022
    Publication date: March 2, 2023
    Inventors: Sinan ARSLAN, Molly HE, Michael PREVITE, Ramreddy TIPPANA, Hua YU, William LIGHT, Junhua ZHAO
  • Publication number: 20230038526
    Abstract: Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one organic solvent having a dielectric constant in the range of no greater than 115; and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.
    Type: Application
    Filed: March 15, 2022
    Publication date: February 9, 2023
    Inventors: Sinan ARSLAN, Molly HE, Michael PREVITE
  • Patent number: 11560552
    Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.
    Type: Grant
    Filed: August 9, 2021
    Date of Patent: January 24, 2023
    Assignees: Illumina, Inc., Illumina Singapore Pte. Ltd.
    Inventors: Misha Golynskiy, Seth McDonald, Saurabh Nirantar, Matthew Kellinger, Michael Previte, Sergio Peisajovich, Molly He
  • Patent number: 11535892
    Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g.
    Type: Grant
    Filed: July 15, 2021
    Date of Patent: December 27, 2022
    Assignee: Element Biosciences, Inc.
    Inventors: Sinan Arslan, Junhua Zhao, Molly He, Samantha Snow, William Light, Matthew Kellinger, Michael Previte, Michael Kim, Hua Yu, Yu-Hsien Hwang-Fu, Marco Tjioe, Andrew Boddicker
  • Publication number: 20220403445
    Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g.
    Type: Application
    Filed: November 8, 2021
    Publication date: December 22, 2022
    Inventors: Sinan ARSLAN, Junhua ZHAO, Molly HE, Samantha SNOW, William LIGHT, Matthew KELLINGER, Michael PREVITE, Michael KIM, Hua YU, Yu-Hsien HWANG-FU, Marco TJIOE, Andrew BODDICKER
  • Publication number: 20220403463
    Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g.
    Type: Application
    Filed: July 15, 2021
    Publication date: December 22, 2022
    Inventors: Sinan ARSLAN, Junhua ZHAO, Molly HE, Samantha SNOW, William LIGHT, Matthew KELLINGER, Michael PREVITE, Michael KIM, Hua YU, Yu-Hsien HWANG-FU, Marco TJIOE, Andrew BODDICKER
  • Publication number: 20220333178
    Abstract: A method for seeding and amplifying target nucleic acids derived from a sample in a cluster at a site on a surface of a substrate includes retaining at least a portion of the target nucleic acids in an inactive form that cannot seed to provide a relatively low concentration of active form target nucleic acids available for seeding. As the active form target nucleic acids seed on the surface of the substrate, they may be amplified. Because the concentration of active form target nucleic acids is low, the likelihood is low that a second active form target nucleic acid will seed at the same site within the same cluster before the first active form target nucleic acid is sufficiently amplified to dominate. Accordingly, the likelihood that the cluster will pass filters is increased relative to traditional seeding and amplification methods employing a higher concentration of active form target nucleic acids.
    Type: Application
    Filed: March 21, 2022
    Publication date: October 20, 2022
    Inventors: Gary Mark Skinner, Geraint Evans, Niall Gormley, Jonathan Boutell, Matthew W. Kellinger, Michael Previte, Molly He
  • Patent number: 11459608
    Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.
    Type: Grant
    Filed: July 9, 2021
    Date of Patent: October 4, 2022
    Assignee: ELEMENT BIOSCIENCES, INC.
    Inventors: Steve Xiangling Chen, Minghao Guo, Michael Previte, Chunhong Zhou, Derek Fuller
  • Patent number: 11447756
    Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.
    Type: Grant
    Filed: March 20, 2020
    Date of Patent: September 20, 2022
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Peter Vander Horn, Cheng-Yao Chen, Guobin Luo, Michael Previte, Jamshid Temirov, Theo Nikiforov, Zhaohui Zhou, Hongye Sun, Yufang Wang, Stefanie Yukiko Nishimura, Hongyi Wang, Marian Peris, Barnett Rosenblum, Michael Phelan
  • Patent number: 11427855
    Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g.
    Type: Grant
    Filed: July 15, 2021
    Date of Patent: August 30, 2022
    Assignee: Element Biosciences, Inc.
    Inventors: Sinan Arslan, Junhua Zhao, Molly He, Samantha Snow, William Light, Matthew Kellinger, Michael Previte, Michael Kim, Hua Yu, Yu-Hsien Hwang-Fu, Marco Tjioe, Andrew Boddicker
  • Patent number: 11426732
    Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.
    Type: Grant
    Filed: October 28, 2020
    Date of Patent: August 30, 2022
    Assignee: ELEMENT BIOSCIENCES, INC.
    Inventors: Minghao Guo, Leon Zilun Zhang, Chunhong Zhou, Matthew Kellinger, Michael Previte
  • Publication number: 20220267842
    Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.
    Type: Application
    Filed: May 13, 2022
    Publication date: August 25, 2022
    Inventors: Steve Xiangling CHEN, Minghao GUO, Michael PREVITE, Chunhong ZHOU, Derek FULLER
  • Publication number: 20220251644
    Abstract: Imaging systems and methods comprising imaging a first interior surface and a second interior surface of a flow cell are described. In some embodiments, the imaging systems may comprise: a) an objective lens; b) at least one image sensor; and c) at least one tube lens disposed in an optical path between the objective lens and the at least one image sensor; wherein the at least one tube lens is configured to correct imaging performance such that images of the first interior surface of the flow cell and the second interior surface of the flow cell have substantially the same optical resolution.
    Type: Application
    Filed: April 18, 2022
    Publication date: August 11, 2022
    Inventors: Steve Xiangling CHEN, Minghao GUO, Michael PREVITE, Chunhong ZHOU, Derek FULLER
  • Publication number: 20220251643
    Abstract: Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm2; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution.
    Type: Application
    Filed: April 18, 2022
    Publication date: August 11, 2022
    Inventors: Steve Xiangling CHEN, Minghao GUO, Michael PREVITE, Chunhong ZHOU, Derek FULLER
  • Patent number: 11408032
    Abstract: Imaging systems and methods comprising imaging a first interior surface and a second interior surface of a flow cell are described. In some embodiments, the imaging systems may comprise: a) an objective lens; b) at least one image sensor; and c) at least one tube lens disposed in an optical path between the objective lens and the at least one image sensor; wherein the at least one tube lens is configured to correct imaging performance such that images of the first interior surface of the flow cell and the second interior surface of the flow cell have substantially the same optical resolution.
    Type: Grant
    Filed: September 9, 2020
    Date of Patent: August 9, 2022
    Assignee: ELEMENT BIOSCIENCES, INC.
    Inventors: Steve Xiangling Chen, Minghao Guo, Michael Previte, Chunhong Zhou, Derek Fuller
  • Patent number: 11365444
    Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.
    Type: Grant
    Filed: July 9, 2021
    Date of Patent: June 21, 2022
    Assignee: ELEMENT BIOSCIENCES, INC.
    Inventors: Steve Xiangling Chen, Minghao Guo, Michael Previte, Chunhong Zhou, Derek Fuller
  • Publication number: 20220186310
    Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.
    Type: Application
    Filed: October 26, 2021
    Publication date: June 16, 2022
    Inventors: Sinan Arslan, Molly Min HE, Matthew KELLINGER, Jake LEVIEUX, Michael PREVITE, Junhua ZHAO, Su ZHANG, Tyler LOPEZ
  • Publication number: 20220170919
    Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.
    Type: Application
    Filed: February 18, 2022
    Publication date: June 2, 2022
    Inventors: Michael PREVITE, Molly HE, Junhua ZHAO, Hui Zhen MAH, Chunhong ZHOU, Sinan ARSLAN, Matthew KELLINGER, Lorenzo BERTI, Steve Xiangling CHEN
  • Patent number: 11339433
    Abstract: Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm2; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution.
    Type: Grant
    Filed: October 7, 2021
    Date of Patent: May 24, 2022
    Assignee: ELEMENT BIOSCIENCES, INC.
    Inventors: Steve Xiangling Chen, Minghao Guo, Michael Previte, Chunhong Zhou, Derek Fuller
  • Publication number: 20220136047
    Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.
    Type: Application
    Filed: January 12, 2022
    Publication date: May 5, 2022
    Inventors: Steve Xiangling CHEN, Minghao GUO, Michael PREVITE, Chunhong ZHOU, Derek FULLER