Publication number: 20190367966
Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including characterization of major histocompatibility complex (MHC) molecules. The compositions, methods, systems, and devices disclosed herein generally describe peptides and barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing MHC molecules from one or more cells.
Type:
Application
Filed:
April 4, 2019
Publication date:
December 5, 2019
Inventors:
Kamila Belhocine, Jason Bell, Zachary Bent, Rajiv Bharadwaj, Christopher Hindson, Mohammad Rahimi Lenji, Bill Kengli Lin, Anthony Makarewicz, Geoffrey McDermott, Elliott Meer, Francesca Meschi, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Michael Schnall-Levin, Sarah Taylor, Jessica Michele Terry, Tobias Daniel Wheeler, Yifeng Yin, Xinying Zheng, Solongo Batjargal Ziraldo, Eswar Prasad Ramachandran Iyer, Luigi Jhon Alvarado Martinez
Publication number: 20190361010
Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from one or more cells. Such polynucleotide processing may be useful for a variety of applications, including generation of labeled macromolecules, including major mistocompatability complex (MHC) molecules, dextramers, etc. Labeled macromolecules may be generated using an in vitro transcription reaction. Labeled macromolecules may be generated in one or more partitions.
Type:
Application
Filed:
April 4, 2019
Publication date:
November 28, 2019
Inventors:
Kamila Belhocine, Jason Bell, Zachary Bent, Rajiv Bharadwaj, Christopher Hindson, Mohammad Rahimi Lenji, Bill Kengli Lin, Anthony Makarewicz, Geoffrey McDermott, Elliott Meer, Francesca Meschi, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Michael Schnall-Levin, Sarah Taylor, Jessica Michele Terry, Tobias Daniel Wheeler, Yifeng Yin, Xinying Zheng, Solongo Batjargal Ziraldo, Eswar Prasad Ramachandran Iyer, Luigi Jhon Alvarado Martinez