Abstract: Disclosed are methods of treating diseases or disorders associated with the expression of Ubiquitin Specific Peptidase 22 (USP22). The disclosed methods may be utilized to treat diseases or disorders associated with cell proliferation, including cancer. Also disclosed are inhibitors of USP22 that specifically inhibit the EC:3.4.19.12 activity, or the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal glycine of ubiquitin. The disclosed compounds may also be used in pharmaceutical compositions and methods for treatment of cell proliferative diseases or disorders associated with USP22 activity.

Abstract: The provided is an ergonomic magnetic thermal garment. An elastic adjustment piece is arranged at a middle opening of a jacket to adapt to different body shapes; both the jacket and a pair of pants are provided with magnetic thermal devices. The magnetic thermal devices include a magnetic control mechanism, an electric heating mechanism, and a control mechanism. The electric heating mechanism is arranged on an inner lining of the jacket and the pants and is connected to the control mechanism. The magnetic control mechanism is arranged on an outer lining of the jacket and the pants and is connected to the control mechanism. The control mechanism is also connected to an external power supply mechanism for providing a power supply. A magnetic thermal garment using a magnetic thermal treatment method is designed, so as to provide magnetic thermal intervention of varying durations and intensities to damaged tissue.

Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.

Abstract: Systems and methods are provided for increasing a quality of computed tomography (CT) images. In one embodiment, a computed tomography (CT) detector system comprises a layer of energy integrating detectors (EID) arranged below a layer of photon counting (PC) sensors with respect to an incoming x-ray, where a number of the PC sensors exceeds a number of the EID detectors; and an image processing unit configured to correct PC data using EID data, and denoise and increase a resolution of an image reconstructed from EID data and PC data using a deep learning convolutional neural network (CNN) trained on pairs of images, each pair of images including a target image reconstructed from a first signal from the layer of PC sensors, and an input image reconstructed from a second signal from the layer of EID detectors, the EID data and PC data acquired concurrently from a same patient ray path.

Abstract: Provided are a verification code processing method and apparatus, and a device and a storage medium. The method comprises: upon receiving a trigger operation for verification code display, firstly acquiring a verification code image and verification code input prompt information; and then displaying the verification code image and the verification code input prompt information for verification code input. In the embodiments of the present disclosure, since a verification code image comprises one or more verification codes, at least one verification code is divided into a plurality of areas, and at least two areas are different in color, the overall structure of the verification code is destroyed, thereby improving the OCR resistance performance of the verification code, and achieving the purpose of effectively intercepting malicious behavior.

Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

Abstract: The present disclosure relates to circular, non-viral DNA vectors, compositions including one or more of the disclosed vectors, and methods for delivering and/or expressing one or more therapeutic genes (e.g., proteins) in mammals, e.g., human patients. In some embodiments, the present disclosure is directed to circular, non-viral DNA vectors, such as circular non-viral DNA vectors including at least two inverted repeat sequences, where the at least two inverted repeat sequences are separated by a non-repeated nucleotide sequence which is not part of the at least two inverted repeat sequences. In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element.” In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element,” but include at least a portion of a bacterial origin of replication.

Abstract: The present disclosure relates to circular, non-viral DNA vectors, compositions including one or more of the disclosed vectors, and methods for delivering and/or expressing one or more therapeutic genes (e.g., proteins) in mammals, e.g., human patients. In some embodiments, the present disclosure is directed to circular, non-viral DNA vectors, such as circular non-viral DNA vectors including at least two inverted repeat sequences, where the at least two inverted repeat sequences are separated by a non-repeated nucleotide sequence which is not part of the at least two inverted repeat sequences. In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element.” In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element,” but include at least a portion of a bacterial origin of replication.

Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

Abstract: LLMs use probabilistic methods to create coherent responses, sometimes going beyond the training data. This can result in “LLM hallucination.” Asset metadata is used in constructing prompts for the LLM, enhancing the LLM's understanding of available assets. Including a limited set of candidate assets in an LLM prompt template as part of a chain-of-thought prompt can solve the hallucination issue. Multiple rounds of interaction with the LLM may be used, allowing for more dynamic and responsive user engagement. The LLM-based recommender system for data catalogs may comprise asset metadata, user data, and a recommender model. The output of the LLM-based recommender system is a recommended asset list. The prompts may explicitly instruct the LLM to limit elements of the list to the candidate set of assets. The LLM's advanced context understanding and reasoning abilities enable it to deliver accurate and interpretable personalized recommendations.

Abstract: System, method, and various embodiments for data tagging and prompt generation are described herein. An embodiment operates by receiving input data, identifying metadata, generating one or more statistics based on the input data, calculating a sample size for the input data based on the one or more statistics and extracting a sample of the input data of the sample size. A prompt is generated based on a prompt template, and the prompt is provided to a language model configured to tag the input in accordance with the prompt. The output including tagged input data is received, and a query is executed against the tagged input data.

Abstract: Systems and methods are provided for increasing a quality of computed tomography (CT) images. In one embodiment, a computed tomography (CT) detector system comprises a layer of energy integrating detectors (EID) arranged below a layer of photon counting (PC) sensors with respect to an incoming x-ray, where a number of the PC sensors exceeds a number of the EID detectors; and an image processing unit configured to correct PC data using EID data, and denoise and increase a resolution of an image reconstructed from EID data and PC data using a deep learning convolutional neural network (CNN) trained on pairs of images, each pair of images including a target image reconstructed from a first signal from the layer of PC sensors, and an input image reconstructed from a second signal from the layer of EID detectors, the EID data and PC data acquired concurrently from a same patient ray path.

Abstract: This disclosure provides compounds of Formula (I) (e.g., Formula (I-aa) (e.g., Formula (I-aa-1), (I-aa-2), (I-aa-3), (I-aa-4), (I-aa-5), or (I-aa-6)), Formula (I-a) (e.g., Formula (I-a-1), (I-a-2), (I-a-3), (I-a-4), (I-a-5), or (I-a-6)), Formula (I-bb) (e.g., Formula (I-bb-1) or (I-bb-2)), or Formula (I-b) (e.g., Formula (I-b-1) or (I-b-2))) or Formula (II), or a pharmaceutically acceptable salt thereof, that induce degradation of a BCL6 protein. These compounds are useful, for example, for treating a cancer in a subject (e.g., a human). This disclosure also provides compositions containing the compounds provided herein as well as methods of using and making the same.

Abstract: This disclosure provides compounds of Formula (I) (e.g., Formula (I-aa) (e.g., Formula (I-aa-1), (I-aa-2), (I-aa-3), or (I-aa-4)), Formula (I-a) (e.g., Formula (I-a-1), (I-a-2), (I-a-3), or (I-a-4)), Formula (I-bb) (e.g., Formula (I-bb-1) or (I-bb-2)), or Formula (I-b) (e.g., Formula (I-b-1) or (I-b-2))) or Formula (II), or a pharmaceutically acceptable salt thereof, that induce degradation of a BCL6 protein. These compounds are useful, for example, for treating a cancer in a subject (e.g., a human). This disclosure also provides compositions containing the same as well as methods of using and making the same.

Abstract: An automated robotic pipettor and reagent cocktail mixture maker includes a pipette rack to hold pipette tips; reagent racks to hold reagent containers; a buffer rack to hold buffer containers; one or more rotatable test tube mixers to hold one or more mixture test tubes; and an automated robotic arm having an automated syringe pump with an end effector to receive a disposable pipette tip. The automated robotic arm moves pipette tips while the automated syringe pump draws measures of liquid from the various containers into the pipette tip and expels it therefrom into a mixture test tube. The rotatable test tube mixer rotates the mixing tube to mix reagents and buffers together. A cooling system cools the buffers, the reagents, and the reagent mix. But for small openings to receive the pipette tip, a hinged cover covers over the buffers, reagents, and reagent mix to deter cross contamination.

Abstract: A laser velocimetry method and system are provided. In the method, an ultrashort pulse laser is subjected to temporal broadening, beam splitting and spectrum broadening in sequence to from a three-dimensional measurement space. When an object moves in the measurement space, a first signal light s_1, a second signal light s_2, a third signal light s_3 are generated, based on which velocity components vy, vx, and vx of the target object can be obtained, respectively, so as to obtain the velocity of the object in accordance with a formula of v=vx·i+vy·j+vz·k.

Abstract: In accordance with one embodiment, a method comprises exciting differing fluorochromes to fluorescence that mark a plurality of moving cells; collecting a broadband light signal having a broadband wavelength range from the fluorescence of the differing fluorochromes; splitting out wavelength ranges in the broadband light signal into smaller wavelength ranges in light signal paths; separately detecting, with differing photo detectors, the light signals in each of the smaller wavelength ranges in the light signal paths; generating digital signals, with analog to digital converters, from the detected light signals in the smaller wavelength ranges in the light signal paths; aligning the digital signals over the broadband wavelength range; and combining the aligned digital signals together into a full spectral response based on the broadband light signal having the broadband wavelength range.

Abstract: The present disclosure relates to circular, non-viral DNA vectors, compositions including one or more of the disclosed vectors, and methods for delivering and/or expressing one or more therapeutic genes (e.g., proteins) in mammals, e.g., human patients. In some embodiments, the present disclosure is directed to circular, non-viral DNA vectors, such as circular non-viral DNA vectors including at least two inverted repeat sequences, where the at least two inverted repeat sequences are separated by a non-repeated nucleotide sequence which is not part of the at least two inverted repeat sequences. In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element.” In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element,” but include at least a portion of a bacterial origin of replication.

Abstract: The present disclosure relates to circular, non-viral DNA vectors, compositions including one or more of the disclosed vectors, and methods for delivering and/or expressing one or more therapeutic genes (e.g., proteins) in mammals, e.g., human patients. In some embodiments, the present disclosure is directed to circular, non-viral DNA vectors, such as circular non-viral DNA vectors including at least two inverted repeat sequences, where the at least two inverted repeat sequences are separated by a non-repeated nucleotide sequence which is not part of the at least two inverted repeat sequences. In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element.” In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element,” but include at least a portion of a bacterial origin of replication.

Abstract: A flow cytometer or cell sorting system includes a linear array detector having a plurality of detectors; a plurality of low noise gain amplifiers respectively coupled to the plurality of detectors in the linear array detector; a plurality of analog to digital converters respectively coupled to the plurality of detectors of the linear array detector; and image reconstruction logic coupled to the plurality of analog to digital converters. The linear array detector receives the brightfield image of each cell of the plurality of biological cells and transduces the light into analog signals representative of pixels in a plurality of brightfield image lines of each cell. The plurality of analog to digital converters transduce the analog signals into digital numeric signals for each pixel. The image reconstruction logic reconstructs the plurality of brightfield image lines of each cell over time periods into a single overall brightfield image of each cell.