Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a compact light detection module including a first image array having a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side.

Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.

Abstract: A computed tomography (CT) imaging system includes a polychromatic X-ray source, a photon counting detector configured to detect X-ray photons traversing a subject and one or more radiocontrast agents administered to the subject and bin the detected X-ray photons across a plurality of energy bins based on energies of the detected X-ray photons (where one or more radiocontrast agents include one or more K-edges in the diagnostic imaging energy range), memory including reconstruction bin weights configured to enhance one or more K-edges, suppress one or more K-edges, or enhance one or more K-edges and suppress one or more K-edges, and a reconstructor configured to apply the reconstruction bin weights to the plurality of energy bins, combine the weighted energy bins into a single set of data, and reconstruct the single set of data to generate K-edge enhanced volumetric image data that enhances at least one K-edge.

Abstract: This disclosure provides compounds of Formula (I) (e.g., Formula (I-1), Formula (I-a) (e.g., Formula (I-a-1), (I-a-2), (I-a-3), (I-a-4), (I-a-5), or (I-a-6)), or Formula (I-b) (e.g., Formula (I-b-1) or (I-b-2))), or a pharmaceutically acceptable salt thereof, that induce degradation of a BCL6 protein. These compounds are useful, for example, for treating a cancer in a subject (e.g., a human). This disclosure also provides compositions containing the same as well as methods of using and making the same.

Abstract: Embodiments of a fluorescent non-conjugated polymer tandem dye nanoparticle is disclosed. In some embodiments, the fluorescent non-conjugated polymer tandem dye nanoparticle comprise a non-conjugated polymer backbone coupled to one or more donor monomer, FRET monomer, acceptor dye, and functional group. In some embodiments, the donor, FRET monomer, and functional group are formed into nanoparticles and the acceptor dye is grafted on after formation of the nanoparticle. In other embodiments, the donor and functional group form nanoparticles, the acceptor dye is grafted on after formation of the nanoparticle. Antibodies can be bioconjugated to the fluorescent non-conjugated polymer tandem dye nanoparticle for use in full spectrum flow cytometry.

Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

Abstract: Disclosed are methods of treating diseases or disorders associated with the expression of Ubiquitin Specific Peptidase 22 (USP22). The disclosed methods may be utilized to treat diseases or disorders associated with cell proliferation, including cancer. Also disclosed are inhibitors of USP22 that specifically inhibit the EC:3.4.19.12 activity, or the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal glycine of ubiquitin. The disclosed compounds may also be used in pharmaceutical compositions and methods for treatment of cell proliferative diseases or disorders associated with USP22 activity.

Abstract: The provided is an ergonomic magnetic thermal garment. An elastic adjustment piece is arranged at a middle opening of a jacket to adapt to different body shapes; both the jacket and a pair of pants are provided with magnetic thermal devices. The magnetic thermal devices include a magnetic control mechanism, an electric heating mechanism, and a control mechanism. The electric heating mechanism is arranged on an inner lining of the jacket and the pants and is connected to the control mechanism. The magnetic control mechanism is arranged on an outer lining of the jacket and the pants and is connected to the control mechanism. The control mechanism is also connected to an external power supply mechanism for providing a power supply. A magnetic thermal garment using a magnetic thermal treatment method is designed, so as to provide magnetic thermal intervention of varying durations and intensities to damaged tissue.

Abstract: A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.

Abstract: Systems and methods are provided for increasing a quality of computed tomography (CT) images. In one embodiment, a computed tomography (CT) detector system comprises a layer of energy integrating detectors (EID) arranged below a layer of photon counting (PC) sensors with respect to an incoming x-ray, where a number of the PC sensors exceeds a number of the EID detectors; and an image processing unit configured to correct PC data using EID data, and denoise and increase a resolution of an image reconstructed from EID data and PC data using a deep learning convolutional neural network (CNN) trained on pairs of images, each pair of images including a target image reconstructed from a first signal from the layer of PC sensors, and an input image reconstructed from a second signal from the layer of EID detectors, the EID data and PC data acquired concurrently from a same patient ray path.

Abstract: Provided are a verification code processing method and apparatus, and a device and a storage medium. The method comprises: upon receiving a trigger operation for verification code display, firstly acquiring a verification code image and verification code input prompt information; and then displaying the verification code image and the verification code input prompt information for verification code input. In the embodiments of the present disclosure, since a verification code image comprises one or more verification codes, at least one verification code is divided into a plurality of areas, and at least two areas are different in color, the overall structure of the verification code is destroyed, thereby improving the OCR resistance performance of the verification code, and achieving the purpose of effectively intercepting malicious behavior.

Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

Abstract: The present disclosure relates to circular, non-viral DNA vectors, compositions including one or more of the disclosed vectors, and methods for delivering and/or expressing one or more therapeutic genes (e.g., proteins) in mammals, e.g., human patients. In some embodiments, the present disclosure is directed to circular, non-viral DNA vectors, such as circular non-viral DNA vectors including at least two inverted repeat sequences, where the at least two inverted repeat sequences are separated by a non-repeated nucleotide sequence which is not part of the at least two inverted repeat sequences. In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element.” In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element,” but include at least a portion of a bacterial origin of replication.

Abstract: The present disclosure relates to circular, non-viral DNA vectors, compositions including one or more of the disclosed vectors, and methods for delivering and/or expressing one or more therapeutic genes (e.g., proteins) in mammals, e.g., human patients. In some embodiments, the present disclosure is directed to circular, non-viral DNA vectors, such as circular non-viral DNA vectors including at least two inverted repeat sequences, where the at least two inverted repeat sequences are separated by a non-repeated nucleotide sequence which is not part of the at least two inverted repeat sequences. In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element.” In some embodiments, the disclosed circular, non-viral DNA vectors do not include a “DD element,” but include at least a portion of a bacterial origin of replication.

Abstract: In one embodiment, a method of building an optimized color flow cytometry panel is disclosed using a full spectrum flow cytometer with five excitation lasers and five corresponding detection modules. In another embodiment, a graphical user interface is disclosed generated by a server computer from a fluorochrome database and displayed by a client computer to assist in the selection of a set of fluorochromes for use in an assay to analyze biological samples. The GUI can display spectra graphs to visually show how fluorochromes may overlap and can generate similarity indexes for the paired fluorochrome interference and a complexity index for overall many to many interferences generated by a selected group or set of fluorochromes.

Abstract: LLMs use probabilistic methods to create coherent responses, sometimes going beyond the training data. This can result in “LLM hallucination.” Asset metadata is used in constructing prompts for the LLM, enhancing the LLM's understanding of available assets. Including a limited set of candidate assets in an LLM prompt template as part of a chain-of-thought prompt can solve the hallucination issue. Multiple rounds of interaction with the LLM may be used, allowing for more dynamic and responsive user engagement. The LLM-based recommender system for data catalogs may comprise asset metadata, user data, and a recommender model. The output of the LLM-based recommender system is a recommended asset list. The prompts may explicitly instruct the LLM to limit elements of the list to the candidate set of assets. The LLM's advanced context understanding and reasoning abilities enable it to deliver accurate and interpretable personalized recommendations.

Abstract: System, method, and various embodiments for data tagging and prompt generation are described herein. An embodiment operates by receiving input data, identifying metadata, generating one or more statistics based on the input data, calculating a sample size for the input data based on the one or more statistics and extracting a sample of the input data of the sample size. A prompt is generated based on a prompt template, and the prompt is provided to a language model configured to tag the input in accordance with the prompt. The output including tagged input data is received, and a query is executed against the tagged input data.

Abstract: Systems and methods are provided for increasing a quality of computed tomography (CT) images. In one embodiment, a computed tomography (CT) detector system comprises a layer of energy integrating detectors (EID) arranged below a layer of photon counting (PC) sensors with respect to an incoming x-ray, where a number of the PC sensors exceeds a number of the EID detectors; and an image processing unit configured to correct PC data using EID data, and denoise and increase a resolution of an image reconstructed from EID data and PC data using a deep learning convolutional neural network (CNN) trained on pairs of images, each pair of images including a target image reconstructed from a first signal from the layer of PC sensors, and an input image reconstructed from a second signal from the layer of EID detectors, the EID data and PC data acquired concurrently from a same patient ray path.

Abstract: This disclosure provides compounds of Formula (I) (e.g., Formula (I-aa) (e.g., Formula (I-aa-1), (I-aa-2), (I-aa-3), (I-aa-4), (I-aa-5), or (I-aa-6)), Formula (I-a) (e.g., Formula (I-a-1), (I-a-2), (I-a-3), (I-a-4), (I-a-5), or (I-a-6)), Formula (I-bb) (e.g., Formula (I-bb-1) or (I-bb-2)), or Formula (I-b) (e.g., Formula (I-b-1) or (I-b-2))) or Formula (II), or a pharmaceutically acceptable salt thereof, that induce degradation of a BCL6 protein. These compounds are useful, for example, for treating a cancer in a subject (e.g., a human). This disclosure also provides compositions containing the compounds provided herein as well as methods of using and making the same.