Abstract: The present disclosure provides expression vectors comprising at least two nucleic acid sequences, namely a nucleic acid sequence encoding an anti-HPRT RNAi, and a nucleic acid sequence encoding a gamma globin gene. In some embodiments, the viral vector is a self-inactivating lentiviral vector. In some embodiments, the gamma-globin gene is used to genetically correct sickle cell disease or ?-thalassemia or to reduce symptoms thereof.

Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.

Abstract: The disclosed methods are generally directed to preventing, treating, suppressing, controlling or otherwise mitigating side effects of T-cell therapy, the T-cell therapy designed to accelerate immune reconstitution, induce a graft-versus-malignancy effect, and/or target tumor cells. In some embodiments, the present disclosure provides expression vectors including a first expression control sequence operably linked to a first nucleic acid sequence, the first nucleic acid sequence encoding a shRNA to knockdown hypoxanthine-guanine phosphoribosyl transferase.

Abstract: The present disclosure is directed to polymer nanocapsules including a ribonucleoprotein complex, and methods of their delivery. In some embodiments, the polymer nanocapsule comprises a polymer shell and a ribonucleoprotein complex, wherein the polymer shell comprises at least one positively charged monomer, at least one neutral monomer, and a crosslinker. Also disclosed are conjugates of polymer nanocapsules coupled to one or more targeting moieties and/or one or more stabilizing moieties.

Abstract: The present disclosure discloses a method and apparatus for preparing a femtosecond optical filament interference direct writing volume grating/chirped volume grating. The method is characterized in that optical filaments are formed in glass by using femtosecond pulse laser, and plasma is controlled to quickly scan in the glass and etch a volume grating or chirped volume grating structure by adjusting the focal length of convex lens, laser energy and movement of motor machine. The apparatus includes a femtosecond pulse laser module, a pulse chirp management module, a pulse time domain shaping module, a laser separation and interference module, a glass volume grating processing platform module and a camera online imaging module.

Abstract: The present disclosure discloses a method and apparatus for manufacturing a microfluidic chip with a femtosecond plasma grating. The method is characterized in that two or more beams of femtosecond pulse laser act on quartz glass together at a certain included angle and converge in the quartz glass, and when pulses achieve synchronization in time domain, the two optical pulses interfere; Benefited by constraint of an interference field, only one optical filament is formed in one interference period; and numbers of optical filaments are arranged equidistantly in space to form the plasma grating. The apparatus for manufacturing the microfluidic chip includes a plasma grating optical path, a microchannel processing platform, and a hydrofluoric acid ultrasonic cell.

Abstract: The present disclosure provides expression vectors comprising at least two nucleic acid sequences, namely a nucleic acid sequence encoding an anti-HPRT RNAi, and a nucleic acid sequence encoding a Wiskott-Aldrich Syndrome protein. In some embodiments, the expression vector is a self-inactivating lentiviral vector. In some embodiments, the Wiskott-Aldrich Syndrome protein is used to alleviate the pathologies associated with Wiskott-Aldrich Syndrome.

Abstract: Provided are a method and a system for measuring a transient time width of an ultrashort pulse in real time. The method includes: performing an interaction of a laser pulse to be measured with a linear chirped pulse in a second-order non-linear medium, to generate a sum-frequency beam, wherein an intensity sag occurs in the chirped pulse after the interaction; performing a time spreading by a time stretching system on the chirped pulse with the intensity sag; detecting the spread chirped pulse with the spread intensity sag by a photoelectric detector, and measuring and recording a time width ?? of the spread intensity sag by an oscilloscope; and obtaining the transient time width ? of the laser pulse to be measured according to a formula of ?=??/M, where M is an amplification coefficient of the time stretching system.

Abstract: Techniques for inferring data to improve the accuracy and completeness of information retrieval are disclosed herein. In some embodiments, a data inference system detects a lack of employment type data for a profile of a user on an online service, with the employment type data identifying at least one type of employment in which the user is interested. In some embodiments, based on the detecting of the lack of employment type data for the profile of the user, the data inference system generates the employment type data based on an inference model and inference data, with the inference data comprising at least one of profile data of the user and a history of the user's interactions with the online service, and the data inference system performs a function of the online service using the generated employment type data.

Abstract: Provided is a system for detection and analysis of a fibrous root system architecture of a plant, including a plant pot having at least one slit area extending through a cross section in an axial direction, at least one laser measuring unit, and a data analyzing unit, where each laser measuring unit has a laser transmitter and a receiver disposed corresponding to the slit area in such a manner that a laser beam emitted from the laser transmitter to the receiver goes across the cross section of the plant pot; measurement on all roots in the slit area is realized by a rotating stage, and the laser measuring unit swinging horizontally around the laser transmitter within a predetermined angle range; and the data analyzing unit is configured to perform statistical analysis on the roots of a plant to be measured according to laser measuring results.

Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.

Abstract: A method and structure for compensating tolerances in assembling modules are provided. The method applies to a pressing fixture and a module with a baseline reference plane and an assembly baseline plane. The assembly baseline plane has at least three compensation baseline bumps, having the height greater than the compensation amount. The pressing fixture has a calibration reference plane. The method comprises: adjusting the position of the module or the pressing fixture to make the baseline reference plane parallel to the calibration reference plane; the pressing fixture using the calibration reference plane to press on the compensation baseline bumps, and changing the position of bump top of each compensation baseline bump; removing the pressing fixture from the compensation baseline bumps, and the plurality of the bump tops forming a preliminary baseline plane, and the preliminary baseline plane being parallel to the baseline reference plane.

Abstract: A method and structure for compensating tolerances in assembling modules are provided. The method applies to a pressing fixture and a module with a baseline reference plane and an assembly baseline plane. The assembly baseline plane has at least three compensation baseline bumps, having the height greater than the compensation amount. The pressing fixture has a calibration reference plane. The method comprises: adjusting the position of the module or the pressing fixture to make the baseline reference plane parallel to the calibration reference plane; the pressing fixture using the calibration reference plane to press on the compensation baseline bumps, and changing the position of bump top of each compensation baseline bump; removing the pressing fixture from the compensation baseline bumps, and the plurality of the bump tops forming a preliminary baseline plane, and the preliminary baseline plane being parallel to the baseline reference plane.

Abstract: In one embodiment, a flow cytometer is disclosed having a compact light detection module. The compact light detection module includes an image array with a transparent block, a plurality of micro-mirrors in a row coupled to a first side of the transparent block, and a plurality of filters in a row coupled to a second side of the transparent block opposite the first side. Each of the plurality of filters reflects light to one of the plurality of micro-mirrors and passes light of a differing wavelength range and each of the plurality of micro-mirrors reflects light to one of the plurality of filters, such that incident light into the image array zigzags back and forth between consecutive filters of the plurality of filters and consecutive micro-mirrors of the plurality of micro-mirrors. A radius of curvature of each of the plurality of micro-mirrors images the fiber aperture onto the odd filters and collimates the light beam on the even filters.

Abstract: Various implementations described herein refer to an integrated circuit having a first stage and a second stage. The first stage has a step-down converter coupled to an oscillator between a first voltage supply and a second voltage supply. The second stage is coupled to the first stage, and the second stage has a current bias generator coupled to a diode-connected transistor between the first voltage supply and the second voltage supply. The second stage provides an intermediate voltage to the first stage.

Abstract: Provided is a control method for a parallel MMC unit of a LCC-MMC hybrid cascade converter station. The control strategy includes: 1) numbering all MMC units connected in parallel in a MMC valve manifold; (2) for a MMC unit using a constant direct-current voltage control manner, calculating a direct-current instruction value of the MMC unit according to a direct-current measurement value; (3) for a MMC unit using a constant active power control manner, calculating an active power instruction value of the MMC unit according to the rated capacity of the MMC unit and a direct-current instruction value of a system rectifier station; (4) for the MMC unit using the constant direct-current voltage control manner, correcting a direct-current voltage instruction value of the MMC unit by using the direct-current instruction value and the direct-current measurement value, and controlling the MMC unit according to the corrected direct-current voltage instruction value.

Abstract: The present disclosure provides expression vectors comprising at least two nucleic acid sequences, namely a nucleic acid sequence encoding an anti-HPRT RNAi, and a nucleic acid sequence encoding a gamma globin gene. In some embodiments, the viral vector is a self-inactivating lentiviral vector. In some embodiments, the gamma-globin gene is used to genetically correct sickle cell disease or ?-thalassemia or to reduce symptoms thereof.

Abstract: Provided are a method and a system for measuring a transient time width of an ultrashort pulse in real time. The method includes: performing an interaction of a laser pulse to be measured with a linear chirped pulse in a second-order non-linear medium, to generate a sum-frequency beam, wherein an intensity sag occurs in the chirped pulse after the interaction; performing a time spreading by a time stretching system on the chirped pulse with the intensity sag; detecting the spread chirped pulse with the spread intensity sag by a photoelectric detector, and measuring and recording a time width ?? of the spread intensity sag by an oscilloscope; and obtaining the transient time width ? of the laser pulse to be measured according to a formula of ?=??/M, where M is an amplification coefficient of the time stretching system.

Abstract: Various implementations described herein are directed to multi-stage system. The system may include a first stage having a current bias generator that generates a biasing current. The system may include a second stage that is coupled to the first stage, and the second stage may include a load that utilizes the biasing current generated by the current bias generator.

Abstract: Various implementations described herein refer to an integrated circuit having a first stage and a second stage. The first stage has a step-down converter coupled to an oscillator between a first voltage supply and a second voltage supply. The second stage is coupled to the first stage, and the second stage has a current bias generator coupled to a diode-connected transistor between the first voltage supply and the second voltage supply. The second stage provides an intermediate voltage to the first stage.