Abstract: Disclosed is a method for producing hyaluronidase or a variant thereof. Specifically, the method is capable of changing the N-glycan levels under culture conditions including a controlled concentration of glucose in the culture medium and a decreased culture temperature for a specific culture time period, thereby increasing the specific activity by 10% or more and improving the quality and production yield.

Abstract: Disclosed is a method for producing hyaluronidase or a variant thereof. Specifically, the method is capable of changing the N-glycan levels under culture conditions including a controlled concentration of glucose in the culture medium and a decreased culture temperature for a specific culture time period, thereby increasing the specific activity by 10% or more and improving the quality and production yield.

Abstract: The present disclosure relates to a recombinant PH20 polypeptide in which 1 to 7 amino acid residues are deleted at the N-terminus of a mature animal wild-type PH20, and use thereof. The N-terminal and/or C-terminal cleaved PH20 polypeptide with improved enzymatic activity and productivity presented in the present disclosure may exhibit superior expression and enzymatic activity compared to conventional recombinant PH20 polypeptides, which may result in reduced production costs for industrial use and thus reduced treatment costs.

Abstract: Disclosed is a method for producing hyaluronidase or a variant thereof. Specifically, the method is capable of changing the N-glycan levels under culture conditions including a controlled concentration of glucose in the culture medium and a decreased culture temperature for a specific culture time period, thereby increasing the specific activity by 10% or more and improving the quality and production yield.

Abstract: Disclosed is a pharmaceutical composition containing (a) a drug, (b) a hyaluronidase PH20 or its variant, and (c) a poloxamer-based surfactant. A human PH20 variant contained in the pharmaceutical composition may includes substitution of one or more amino acids at one or more regions selected from an alpha helix 8 sequence (S347 to C381) and a linker (A333 to R346) between alpha helix 7 and alpha helix 8 in a wild-type human PH20 having a sequence of SEQ ID NO: 1 and optionally includes truncation of one or more amino acids at an N-terminus and/or C-terminus in the wild-type human PH20 having a sequence of SEQ ID NO: 1. In addition, the pharmaceutical composition further contains a pharmaceutically acceptable additive, particularly a stabilizer.

Abstract: Disclosed are novel PH20 variants or fragments thereof with improved thermal stability and enzymatic activity of human hyaluronidase, which is an enzyme that hydrolyzes hyaluronic acid, and more particularly novel PH20 variants or fragments thereof including one or more amino acid residue substitutions in the variant having the amino acid sequence of SEQ ID NO: 3, wherein one or more amino acid residues at the N-terminus and/or the C-terminus are optionally further deleted.

Abstract: Disclosed is a method for producing hyaluronidase or a variant thereof. Specifically, the method is capable of changing the N-glycan levels under culture conditions including a controlled concentration of glucose in the culture medium and a decreased culture temperature for a specific culture time period, thereby increasing the specific activity by 10% or more and improving the quality and production yield.

Abstract: Disclosed are an array substrate, a display panel and a method for repairing bad spots on the panel. The array substrate includes a substrate and a plurality of data lines and a plurality of scan lines provided on the substrate, the plurality of the data lines and a plurality of the scan lines are insulated and intersected mutually to define a plurality of sub-pixel areas provided in an array, each sub-pixel area is provided with a sub-pixel, the sub-pixel includes a plurality of pixel electrodes provided in a same layer and spaced to form a plurality of light-emitting areas, two adjacent pixel electrodes are electrically connected to each other via a connection electrode, and a pixel driving circuit is electrically connected to a corresponding data line, a corresponding scan line, and a corresponding pixel electrode.

Abstract: An array substrate, a method of manufacturing the same, and a display panel are disclosed. The method includes: providing a substrate; depositing a light-shielding material on the substrate; and etching the light-shielding material to form a light-shielding layer, where an included angle formed between a side surface of the formed light-shielding layer and a plane where the substrate is located lies in the range of 80 to 110 degrees.

Abstract: Provided herein are a suspension apparatus and a specialized vehicle including the same. The suspension apparatus includes a crankshaft fixed to a vehicle body, a housing rotatably connected to the crankshaft, a first damping portion arranged in the housing and having a damping fluid accommodated in the first damping portion, an amount of the damping fluid in the first damping portion being adjusted according to an external force applied to the vehicle body, a second damping portion arranged in the housing, connected to the first damping portion such that the damping fluid moves between the first damping portion and the second damping portion, and including: a first space accommodating a compressed gas; and a second space accommodating the damping fluid, the first space facing the second space; and a rotational force applier arranged in the housing and configured to apply a rotational force to the housing by adjusting an amount of a working fluid in the rotational force applier.

Abstract: The present invention is related to the field of protein engineering technology which increases the enzymatic activity and thermal stability of human hyaluronidase which is an enzyme that hydrolyzes hyaluronic acid; and more particularly to hyaluronidase PH20 variants or fragments thereof, which comprise one or more amino acid residue substitutions in the region corresponding to the alpha-helix region and its linker region in the amino acid sequence of wild-type PH20 of SEQ ID NO: 1 and in which one or more amino acid residues at the N-terminus and/or the C-terminus are selectively cleaved additionally.

Abstract: Provided herein are a suspension apparatus and a specialized vehicle including the same. The suspension apparatus includes a crankshaft fixed to a vehicle body, a housing rotatably connected to the crankshaft, a first damping portion arranged in the housing and having a damping fluid accommodated in the first damping portion, an amount of the damping fluid in the first damping portion being adjusted according to an external force applied to the vehicle body, a second damping portion arranged in the housing, connected to the first damping portion such that the damping fluid moves between the first damping portion and the second damping portion, and including: a first space accommodating a compressed gas; and a second space accommodating the damping fluid, the first space facing the second space; and a rotational force applier arranged in the housing and configured to apply a rotational force to the housing by adjusting an amount of a working fluid in the rotational force applier.

Abstract: The present invention provides a stable peptide-conjugated, ascorbic acid derivative, a method for preparing the same, and a cosmetic composition comprising the same as an active ingredient. The stable peptide-conjugated ascorbic acid derivative of the present invention has both the effect of whitening the skin by inhibiting melanin production and the effect of reducing skin wrinkles by activating collagen production, and may be used in a cosmetic composition.

Abstract: The present invention provides a stable peptide-conjugated, ascorbic acid derivative, a method for preparing the same, and a cosmetic composition comprising the same as an active ingredient. The stable peptide-conjugated ascorbic acid derivative of the present invention has both the effect of whitening the skin by inhibiting melanin production and the effect of reducing skin wrinkles by activating collagen production, and may be used in a cosmetic composition.

Abstract: The present invention relates to a novel compound or a pharmaceutically acceptable salt thereof inhibiting 11?-HSD1 enzyme activity, a preparation method of the same, and a pharmaceutical composition comprising the same as an active ingredient. Since the compound of the present invention selectively inhibits the activity of 11?-HSD1 (11?-Hydroxysteroid dehydrogenase type 1), the compound of the invention can be effectively used as a therapeutic agent for the treatment of diseases caused by the over-activation of 11?-HSD1 such as non-insulin dependent type II diabetes, insulin resistance, obesity, lipid disorder, metabolic syndrome, and other diseases or condition mediated by the excessive activity of glucocorticoid.

Abstract: The present invention relates to a novel compound or a pharmaceutically acceptable salt thereof inhibiting 11?-HSD1 enzyme activity, a preparation method of the same, and a pharmaceutical composition comprising the same as an active ingredient. Since the compound of the present invention selectively inhibits the activity of 11?-HSD1 (11?-Hydroxysteroid dehydrogenase type 1), the compound of the invention can be effectively used as a therapeutic agent for the treatment of diseases caused by the over-activation of 11?-HSD1 such as non-insulin dependent type II diabetes, insulin resistance, obesity, lipid disorder, metabolic syndrome, and other diseases or condition mediated by the excessive activity of glucocorticoid.

Abstract: Provided is a system for measuring bad breath or blood alcohol concentration. More specifically, the system includes: a portable terminal; a bad breath analyzer which has an alcohol sensor and a bad breath sensor mounted therein, and which is connected to an earphone connection terminal of the portable terminal so as to increase compatibility; and a bad breath processing unit which is provided in the portable terminal, and which processes signals measured from the alcohol sensor and the bad breath sensor to output one or more pieces of drinking information and bad breath information on a screen of the portable terminal.

Abstract: Provided herein include compositions, all related stereoisomers as well as pharmaceutically acceptable salts provided as simplified analogs of pateamine A, in which the analogs generally are devoid of the C3-amino and C5-methyl groups, also referred to as desmethyl, desamino-pateamine A. Suitable analogs provide anticancer and antiproliferative effects in vivo and in vitro by a novel drugs mechanism of action described herein for pateamine A, including inhibition of eIF4A-dependent translation initiation. As with pateamine A, as described herein, suitable analogs cause cell cycle arrest or induce apoptosis in transformed cells. However, toxicity of such compounds to slow growing normal cells is low. In addition, such analogs, like pateamine A, target translation initiation factors and are useful as anticancer and antiproliferative agents in subjects in need thereof.

Abstract: The present invention relates to piperazine dithioctate, a novel addition salt of thioctic acid with a base and a pharmaceutical composition comprising the same. The piperazine dithioctate according to the present invention has good thermal and moisture stability and high water-solubility as well as dosage increase lower than other addition salts, thereby being effectively used for preparing a pharmaceutical composition for antioxidation or for preventing or treating diabetic polyneuropathy, etc.

Abstract: Disclosed are a cleaning robot system and its method for controlling which make it possible to reliably and quickly clean with the aid of the cleaning robot. The cleaning robot system comprises at least one external device each formed of an indicator and a virtual wall setting function, with the aid of which cleaning work can be performed by the unit of blocks about a cleaning area reference point, not leaving non-cleaned area behind. Since the access of the cleaning robot can be restricted by means of a virtual wall set by an external device, any damages of furniture due to a collision of the cleaning robot can be prevented, and the cleaning robot can be barred from escaping from the set cleaning area.