Abstract: A reaction vessel suitable for automating of various measurements is provided. In a preferred embodiment, on the same side of plate-like substrate (10), sample injection part (12), typing reagent reservoir part (14) and mineral oil reservoir part (16) are provided as concaves, and further, multiple probe arrangement parts (18) are provided. The typing reagent reservoir part (14) and mineral oil reservoir part (16) are sealed with film (20). The surface of the substrate (10) is covered with detachable sealing material (22) with a size capable of covering the sample injection part (12), typing reagent reservoir part (14), mineral oil reservoir part (16) and multiple probe arrangement parts (18) in such a manner that the film (20) is covered by the sealing material (22). Liquid transfer is carried out through nozzles.

Abstract: Disclosed herein is a reaction container plate which prevents the entry of foreign matter from the outside and the pollution of an outside environment. A reaction container plate (1) includes a sealed reaction container (5), a reaction container channel (15) connected to the reaction container (5), a sealed container (17) provided separately from the reaction container (5), a sealed container channel (17a) connected to the sealed container (17), a syringe (33) for sending a liquid, a switching valve (47) for connecting the syringe (33) to the introduction channel (15) or the sealed container channel (17a), and a sealed container air vent channel (17b) of which one end is connected to the sealed container (17).

Abstract: In a preferred embodiment, a gene polymorphism diagnosing reaction vessel at least having a plurality of probe arrangement parts each individually holding a probe that emits fluorescence in accordance with each of a plurality of polymorphic sites is used. A controller (118) determines presence or absence of a gene polymorphism based on a fluorescence intensity value (a time-course gradient) per unit time of a fluorescence detection value obtained from a fluorescence detector (64).

Abstract: A surrounding environment and a solution to be dispensed are prevented from being polluted by aerosol inside a dispensation tip. A dispensation tip 20 is provided with a dispensation nozzle 19 attached to its distal end, a syringe having a hollow inner portion, that is connected to a proximal end of the dispensation nozzle 19, and a plunger 22 that is allowed to slide in the cylinder 21 of the syringe so that suction and discharge processes are carried out on the liquid by the dispensation nozzle 19. A separation member having airtightness for separating an inside of the nozzle 19 from the outside and flexibility for allowing the plunger 22 to freely slide is placed between the proximal end of the plunger 22 and the proximal end of the cylinder 21.

Abstract: The processing apparatus for microchips, each at least having a main flow path performing migration of a sample for analysis inside a sheet-like member, comprises a holding part holding microchips so that the multiple main flow paths are provided; a pretreatment part common to the multiple main flow paths for performing a pretreatment step prior to an analysis step in each of the multiple main flow paths; a processing part for performing analysis in each of the main flow paths independently of the others; and a control part controlling an operation in the pretreatment part so that when a pretreatment step for one main flow path ends, a pretreatment for a next main flow path starts and further controlling an operation in the processing part so that an analysis is subsequently performed for the main flow path where the pretreatment step has ended.

Abstract: A reaction container kit in which a judgment can be made easily whether a sample is not yet injected or injected already while a sample is prevented from being injected into an incorrect reaction container erroneously. Before a sample is injected, the bar code (132) of a first bar code label is read out by means of a bar code reader and a judgment is made automatically whether that reaction container is a reaction container for the inspection items requested for the sample to be injected or not. If that reaction container is a correct one, the first bar code label (130) is peeled off and a sample is injected into a sample container (32). Subsequently, a second bar code label (134) is stuck onto the sample container (32). Consequently, an opening (31) is sealed hermetically with the second bar code label (134) and the sample is isolated from the outside under a state where the sample is introduced into the space of the reaction container covered with a cover (24).

Abstract: Disclosed herein is a reactor plate including a sealed reaction well, a reaction well channel connected to the reaction well, and a reaction well air vent channel connected to the reaction well. The reaction well contains a reagent contained in the bottom thereof and a thermally fusible material which is solid at room temperature and is contained in the bottom thereof to encapsulate the reagent. When the reaction well is heated, the thermally fusible material is melted, and as a result, a sample liquid introduced into the reaction well through the reaction well channel reacts with the reagent in the reaction well.

Abstract: The reactor plate preferably includes a sealed reactor, a reactor flow channel connected to the reactor, a sample container constituted from a sealed container provided separately from the reactor, a sample container flow channel to be connected to the sample container, a syringe for sending a liquid, a switching valve for connecting the syringe to the reactor flow channel or the sample container flow channel, and a projecting flow channel connected to the end of the sample container flow channel located on the sample container side. The sample container has a penetrable portion through which the projecting flow channel can penetrate and which is provided to be opposed to the projecting flow channel and is located at a position such that the projecting flow channel penetrating the penetrable portion is brought into contact with a liquid contained in the sample container.

Abstract: A detection chip comprises a substrate and a well-shaped reaction portion formed in the substrate, in which the well-shaped reaction portion comprises a bottom portion having a planar shape and a side portion the width between which is widen from the bottom portion toward an opening portion.

Abstract: The object of the invention is to carry out typing for multiple SNP sites automatically from the stage of sample preparation. A mixture of sample (2) and PCR reaction solution (4) is subjected to PCR reaction according to a given temperature cycle. After the completion of PCR reaction, invader reagent (6) is added thereto. Subsequently, the reaction mixture having the invader reagent (6) added thereto is added to probe fixing part (8) of typing reaction zone to thereby effect reaction therebetween. Invader probes capable of emitting fluorescence in respective correspondence to multiple SNP sites are separately held on individual sites of the probe fixing part (8), so that the reaction mixture reacts with the invader probes and when SNPs corresponding to the invader probes exist, fluorescence is emitted.

Abstract: The present invention provides a simple cell-free protein synthesis method capable of affording synthesis of a protein in a high amount in a short time at a low cost. A method for cell-free protein synthesis using an extract derived from an insect cell, the method comprising removing a component which can pass through a semipermeable membrane through the semipermeable membrane while maintaining synthesis reaction, thereby to continuously synthesize a protein. Preferably, an mRNA is additionally supplied while said synthesis reaction is maintained. Further, said insect cell is preferably an established culture cell derived from Trichoplusia ni ovum cell.

Abstract: The analysis apparatus for microchips, each at least having a main flow path performing migration of a sample for analysis inside a sheet-like member, comprises a holding part holding microchips so that the multiple main flow paths are provided; a pretreatment part common to the multiple main flow paths for performing a pretreatment step prior to an analysis step in each of the multiple main flow paths; a processing part for performing analysis in each of the main flow paths independently of the others; and a control part controlling an operation in the pretreatment part so that when a pretreatment step for one main flow path ends, a pretreatment for a next main flow path starts and further controlling an operation in the processing part so that an analysis is subsequently performed for the main flow path where the pretreatment step has ended.

Abstract: In a separation buffer solution filling device, a microchip is arranged such reservoirs are opened on a surface on respective ends of channels including at least a main separation channel in which analysis is performed while a solution moves inside a plate-like member, and the reservoirs face upward. The filling device fills separation buffer solution into the channels by supplying air from an air supply port which is pushed while maintaining air-tightness onto a top of the reservoir filled with the separation buffer solution on either end of said channels. The air supply port is an opening on a front end of an air cylinder, and has a seal part on that opening. The filling device is pushed onto the reservoir while maintaining air-tightness by that seal part.

Abstract: A microchip processing apparatus processes a microchip with at least one main separation channel. The microchip processing apparatus includes a holding part configured to hold the microchip, a container containing a sample or a reagent, and a dispensing probe having a needle formed on a tip of the dispensing probe. The dispensing probe is actuated to be inserted into the container from above the container, to draw the sample or reagent, and to inject to a prescribed position on the held microchip. A dispensing probe driving mechanism moves the dispensing probe between prescribed positions of the microchip and the container.

Abstract: Methods and apparatuses for dispensing samples or reagents onto a target object are provided. A dispensing position designating section (62) designates the dispensing position on the target object (50), such as a membrane, based on an image of the target object (50) displayed on a monitoring section (60). A dispensing control section (64) positions the target object and the dispensing device relative to each other so that the designated dispensing position on the target object is placed beneath the dispensing device (10) and controls the dispensing operation of the dispensing device (10). A dispensing position information creating section (68) creates dispensing position information about the dispensing position on the target object (50) which is designated by the dispensing position designating section (62) and to which the dispensing operation has been performed and outputs it to the outside.

Abstract: The present invention provides a simple cell-free protein synthesis method capable of affording synthesis of a protein in a high amount in a short time at a low cost. A method for cell-free protein synthesis using an extract derived from an insect cell, the method comprising removing a component which can pass through a semipermeable membrane through the semipermeable membrane while maintaining synthesis reaction, thereby to continuously synthesize a protein. Preferably, an mRNA is additionally supplied while said synthesis reaction is maintained. Further, said insect cell is preferably an established culture cell derived from Trichoplusia ni ovum cell.

Abstract: The processing apparatus for microchips, each at least having a main flow path performing migration of a sample for analysis inside a sheet-like member, comprises a holding part holding microchips so that the multiple main flow paths are provided; a pretreatment part common to the multiple main flow paths for performing a pretreatment step prior to an analysis step in each of the multiple main flow paths; a processing part for performing analysis in each of the main flow paths independently of the others; and a control part controlling an operation in the pretreatment part so that when a pretreatment step for one main flow path ends, a pretreatment for a next main flow path starts and further controlling an operation in the processing part so that an analysis is subsequently performed for the main flow path where the pretreatment step has ended.

Abstract: A sample plate is provided with a mass-spectrometry-use measuring-sample preparation area which serves as an ionization area used for ionizing the sample through laser irradiation, and a membrane affixing area which serves as a plane area on which the membrane bearing the sample adsorbed thereon is fixedly held. The mass-spectrometry-use measuring-sample preparation area is provided with spots at which the sample, extracted from the membrane fixedly affixed to the membrane affixing area, is dropped together with a matrix solution, and placed, and the spots are preferably regularly arranged thereon. Each of the spots preferably has a round structure surrounded by a groove on the periphery thereof so as to be dried in a converged state without being diffused from a fixed area.

Abstract: A sample plate is provided with a mass-spectrometry-use measuring-sample preparation area which serves as an ionization area used for ionizing the sample through laser irradiation, and a membrane affixing area which serves as a plane area on which the membrane bearing the sample adsorbed thereon is fixedly held. The mass-spectrometry-use measuring-sample preparation area is provided with spots at which the sample, extracted from the membrane fixedly affixed to the membrane affixing area, is dropped together with a matrix solution, and placed, and the spots are preferably regularly arranged thereon. Each of the spots preferably has a round structure surrounded by a groove on the periphery thereof so as to be dried in a converged state without being diffused from a fixed area.

Abstract: The object of the present invention is to provide a method for separating components in a liquid specimen and an apparatus used in said method, by which it is possible to avoid carryover even when the specimen injector is not washed using washing solution and also to avoid the increase of inner pressure in column of the separation device or ineffective separating operation even when many liquid specimens are separated by many times using the same separation device. In the present invention, the liquid specimen is introduced into the separation device from a direction opposite to eluting direction of the separation device which retains an adsorbent, and by separating and eluting the specimen from eluting outlet together with the eluant, trace quantity of insoluble substances and the specimen remained on the specimen introducing passage can be removed by elution procedure.