Abstract: The present invention provides a method of detecting oral carcinoma cells with high accuracy, and a method of making possible early detection of oral carcinoma or discrimination between a precancerous lesion and an early cancer in diagnosis of oral carcinoma. The methods are achieved by screening cells for oral carcinoma or precancerous lesion by measuring a DNA copy number in whole chromosomes or a part thereof in a sample, wherein chromosomal regions for which said copy number is measured comprise at least one region selected from the group consisting of: a 22-23 region in the q arm of Chromosome 8, a 14-21 region in the p arm of Chromosome 3 and a 12-22 region in the p arm of Chromosome 5.

Abstract: Provided is a method for performing a hybridization reaction that comprises the steps of providing a sample containing a target single-stranded nucleic acid and a probe array; heat-denaturing the probe array in a solution containing the sample; and reducing temperature to the extent suitable for a double-strand formation reaction, wherein the probe array remains immersed in the sample solution during reducing the temperature. Also disclosed is a method for detecting a certain sequence in a sample.

Abstract: To provide a method of efficiently removing excitation light in a device for measuring fluorescence emitted from samples on a measuring surface of a substrate while illuminating the samples with excitation light. The method is a fluorometry characterized in that an excitation light illumination portion where the samples are illuminated with the excitation light and a light detecting portion where measurements are made of the fluorescence are placed in such a manner as to make it possible to prevent the excitation light from approaching the light detecting portion, and measurements of the fluorescence emitted from the samples on the measuring surface of the substrate are made in such a manner as to relatively move the samples from the excitation light illumination portion to the light detecting portion after illuminating the same with the excitation light.

Abstract: A method for dry detection/quantification of targeted nucleotide chains, comprising the steps of: (1) realizing a state in which a hybrid (C) of a certain amount of targeted nucleotide chain (A), which is derived from a sample solution and subjected to detection or quantification, and a probe nucleotide chain (B), which has a base sequence complementary to a specific site of the base sequence of the targeted nucleotide chain, is formed on a solid-phase substrate by mutually reacting the two types of nucleotide chains with each other, and in which there exists a fluorescence dye (D), which acts on the hybrid (C), thereby emits fluorescence or increases its fluorescence intensity, and is capable of continuing to emit fluorescence even in the dried state while acting on the hybrid; (2) drying the hybrid (C) and the fluorescence dye (D) on the substrate; and (3) measuring the fluorescence emitted from the fluorescence dye (D), as a measuring means, after the drying operation.

Abstract: A method for screening presence or absence of a variation in a region of a nucleic acid which comprises the steps of (a) preparing a sample containing a test nucleic acid corresponding to the region, (b) preparing a probe having a base sequence fully complementary to a normal sequence of the region, and a plurality of probes each having at least one base not complementary to the normal sequence, (c) fixing the probes in separate regions on a surface of a substrate to prepare a DNA array substrate, (d) reacting the test nucleic acid with the probes on the DNA array substrate, (e) measuring signals in each region where the signals are originated from respective hybrids formed between the test nucleic acid and one of the probes, and (f) calling variation in the test nucleic acid using a pattern of total signals of all regions.

Abstract: A method for screening presence or absence of a variation in a region of a nucleic acid which comprises the steps of (a) preparing a sample containing a test nucleic acid corresponding to the region, (b) preparing a probe having a base sequence fully complementary to a normal sequence of the region, and a plurality of probes each having at least one base not complementary to the normal sequence, (c) fixing the probes in separate regions on a surface of a substrate to prepare a DNA array substrate, (d) reacting the test nucleic acid with the probes on the DNA array substrate, (e) measuring signals in each region where the signals are originated from respective hybrids formed between the test nucleic acid and one of the probes, and (f) calling variation in the test nucleic acid using a pattern of total signals of all regions.

Abstract: A database capable of accurately analyzing the relation between physical constitution and diseases based on the amount of expression of MHC genes and disease related genes, and extracting useful medical information is efficiently constructed.

Abstract: An infectious etiologic agent detection probe set which detects an infectious etiologic agent gene, includes a plurality of kinds of probes including oligonucleotide having base sequences selected from each of a plurality of groups selected from a first group including base sequences of SEQ ID Nos. 1 to 14 and complementary sequences thereof, a second group including base sequences of SEQ ID Nos. 15 to 24 and complementary sequences thereof, a third group including base sequences of SEQ ID Nos. 25 to 36 and complementary sequences thereof, a fourth group including base sequences of SEQ ID Nos. 37 to 47 and complementary sequences thereof, a fifth group including base sequences of SEQ ID Nos. 48 to 57 and complementary sequences thereof, a sixth group including base sequences of SEQ ID Nos. 58 to 68 and complementary sequences thereof, a seventh group including base sequences of SEQ ID Nos. 69 to 77 and complementary sequences thereof, an eighth group including base sequences of SEQ ID Nos.

Abstract: To provide a method of making measurements for a sample on the measuring surfaces of a substrate which makes it possible to simplify the control and construction of a measuring device, shorten the measuring period, make the measuring conditions constant, and improve the positional accuracy. The method and a device for carrying out the method are characterized in that measurements for the sample is performed by forming a circular orbit of detection areas, where detection is performed with a detector, on the measuring surfaces of the substrate while moving the detection areas relative to the substrate.

Abstract: A probe array comprises a plurality of probes immobilized at a plurality of matrix sites on a substrate for capturing a target substance, wherein the probes are sequentially synthesized at the matrix sites on the substrate until a desired length, the probes are different from each other, and a labeling compound is coupled to each terminus of the probes in a final step of the synthesis. The probe array of the invention allows sensitive and reliable detection of the target substance. A method of evaluating the amount of the fully synthesized probes at respective matrix sites is also provided.

Abstract: Multiple specimens, typically biological samples having different properties and origins, are bound onto matrix substrates, and oligonucleotides, proteins and drugs are spotted on each matrix in an array to examine those specimens at a time for multiple items.

Abstract: A probe bound substrate allowing us to quickly detect or quantify a target substance or sequence a target nucleic acid at a lower cost is provided. Specifically, there is provided a probe bound substrate in which a probe capable of specifically attaching to a target substance is bound at the first site on its surface, characterized in that a marker is bound at the second site where the first site may be specified.

Abstract: To provide a method of making measurements for a sample on the measuring surfaces of a substrate which makes it possible to simplify the control and construction of a measuring device, shorten the measuring period, make the measuring conditions constant, and improve the positional accuracy. The method and a device for carrying out the method are characterized in that measurements for the sample is performed by forming a circular orbit of detection areas, where detection is performed with a detector, on the measuring surfaces of the substrate while moving the detection areas relative to the substrate.

Abstract: Multiple specimens, typically biological samples having different properties and origins, are bound onto matrix substrates, and oligonucleotides, proteins and drugs are spotted on each matrix in an array to examine those specimens at a time for multiple items.

Abstract: A reaction site array to be used in conducting a plurality of reactions between two or more kinds of substances in a liquid medium with a trace amount is provided. The reaction site array comprises a plurality of reaction sites separated from each other. Each reaction site is composed of a first region and a second region. The second region is raised from the first region to separate the first regions from each other. The second region has an affinity to a liquid medium lower than that of the first region. A preparation process of the reaction site and a reaction process using the reaction site array is also provided.

Abstract: This invention provides a pattern exposure method which may be used for effectively preparing, for example, a DNA or peptide array with a lower cost and a device therefor. Specifically, the method is a method for pattern exposure comprising the step of exposing a photosensitive material on a solid-phase substrate by irradiating it with a light as a pattern, wherein the photosensitive material is selectively exposed with beams selectively emitted from multiple vertical cavity surface emitting laser sources aligned as an array.

Abstract: A method for identifying an unknown base sequence present in a target single-stranded nucleic acid utilizing a probe array in which single-stranded nucleic acid probes are arranged as isolated spots on a substrate, where each probe has a base sequence complementary to one of plural base sequences expected to be the unknown base sequence, and fluorescence pattern of a sample on the probe array is compared with template patterns to know the base sequence of the sample.

Abstract: A reaction site array to be used in conducting a plurality of reactions between two or more kinds of substances in a liquid medium with a trace amount is provided. The reaction site array comprises a plurality of reaction sites separated each other, each of the reaction sites is composed of a first region and a second region, the second region is raised from the first region to separate the first regions each other, and the first region has an affinity to the liquid medium and the second region has an affinity to the liquid medium lower than that of the first region. A preparation process of the reaction site array and a reaction process using the reaction site array are also provided.

Abstract: Provided is a method of spotting a probe densely and efficiently on a surface of a solid support. A liquid containing a probe is attached to a solid support as droplets to form spots containing the probe on the solid support by an ink jet method.

Abstract: A probe array comprises a plurality of probes immobilized at a plurality of matrix sites on a substrate for capturing a target substance, wherein the probes are sequentially synthesized at the matrix sites on the substrate until a desired length, the probes are different from each other, and a labeling compound is coupled to each terminus of the probes in a final step of the synthesis. The probe array of the invention allows sensitive and reliable detection of the target substance. A method of evaluating the amount of the fully synthesized probes at respective matrix sites is also provided.